MO BIO Laboratories PowerLyzer 12155 Instruction Manual

MO BIO Laboratories PowerLyzer 12155 Instruction Manual

24 bench top bead-based homogenizer

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I N S T R U C T I O N
M A N U A L
PowerLyzer
24 Bench Top Bead-Based
Homogenizer
MO BIO Laboratories, Inc.
2746 Loker Avenue West
Carlsbad, CA 92010
P: (760) 929-9911
F: (760) 929-0109
www.mobio.com
13155 rev. 070110

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  • Page 1 I N S T R U C T I O N M A N U A L ™ PowerLyzer 24 Bench Top Bead-Based Homogenizer MO BIO Laboratories, Inc. 2746 Loker Avenue West Carlsbad, CA 92010 P: (760) 929-9911 F: (760) 929-0109 www.mobio.com...
  • Page 2: Warranty Information

    This MO BIO Laboratories, Inc. product is warranted to be free from defects in material and workmanship for a period of ONE YEAR from the date of delivery. MO BIO Laboratories, Inc. will repair or replace and return free of charge any part which is returned to its factory within said period, transportation prepaid by user, and which is found upon inspection to have been defective in materials or workmanship.
  • Page 3 24 Homogenizer. If you ™ experience problems with or have questions about your PowerLyzer 24 Homogenizer, contact ™ Technical Support at MO BIO Laboratories, Inc. at 1-800-606-6246 or 760-929-9911. DANGER  DO NOT allow the machine to be submerged in any liquid. ...
  • Page 4: Table Of Contents

    5.1 TRANSPORTING THE POWERLYZER™ 24 HOMOGENIZER............24  5.2 STORAGE ................................25  SECTION 6. SUPPORT AND SERVICE ................... 25  6.1 ASSISTANCE ................................. 25  6.2 DECONTAMINATION ............................. 25  6.3 DECONTAMINATION CERTIFICATION FORM ..................26  MO BIO Laboratories, Inc. | 1-800-606-6246 | www.mobio.com                    13155 rev. 070110  ...
  • Page 5: Section 1. Powerlyzer ™ 24 Bench Top Bead-Based Homogenizer

      USA: 115V Power Cord European: 230V Power Cord WARNING: Check that the voltage indicated on the rear panel of the unit matches the local power supply. MO BIO Laboratories, Inc. | 1-800-606-6246 | www.mobio.com                    13155 rev. 070110...
  • Page 6: Specifications

    CE certified Approval/Compliance: CAUTION: This is a Class A apparatus. The equipment may cause radio-electric interference in a residential environment. In this case, it is recommended that the user takes appropriate measures. MO BIO Laboratories, Inc. 1-800-606-6246 www.mobio.com             13155 rev. 070110...
  • Page 7: Parts

    1.3 PARTS Prior to operation, please remove all parts from the shipping container and inspect for damaged or missing parts. If any parts are found to be damaged or missing, please contact MO BIO Laboratories, Inc. at 1-800-606-6246. The PowerLyzer 24 Homogenizer consists of the following: ™...
  • Page 8 Hub Lock Switch to lock Finger Plate and secure tubes Tube Holder Finger Plate Air Vent ON/OFF Switch Cooling Fan Main Power Supply Plug Shipping Screws Fuses 115V/230V Voltage Selection Switch MO BIO Laboratories, Inc. 1-800-606-6246 www.mobio.com             13155 rev. 070110...
  • Page 9: Section 2. Set Up And Operation

    Packaging crates may be purchased for a minimal charge. For shipping inquiries, contact MO BIO at 1-800-606-6246.  MO BIO Laboratories, Inc. 1-800-606-6246 www.mobio.com            ...
  • Page 10 WARNING: DO NOT modify the plug or cords that are provided. Damaged or worn power cords should be repaired or replaced immediately by a qualified electrician. MO BIO Laboratories, Inc. 1-800-606-6246 www.mobio.com          ...
  • Page 11: Tube Motion

    Number of Cycles 01 to 10 Dwell/Pause between Cycles Dwell minimum* up to 9:59 Storable Programs 1 to 99 *Dwell minimum varies depending upon the speed and length of cycle. MO BIO Laboratories, Inc. 1-800-606-6246 www.mobio.com             13155 rev. 070110...
  • Page 12: Keypad Layout

    The user can adjust four different homogenization settings with this interface. The UP and DOWN arrow buttons are used to navigate through the menu and the LEFT and RIGHT arrow buttons are used to adjust parameters. MO BIO Laboratories, Inc. 1-800-606-6246 www.mobio.com          ...
  • Page 13: Start Up Of The Equipment

    “D=” denotes the time interval in seconds of dwell/pause between each cycle. The variable can range from “Dmin” (Dwell minimum* expressed as min:sec) to 9:59, in increments of 1 second. *Dwell minimum varies depending upon the speed and length of cycle. MO BIO Laboratories, Inc. 1-800-606-6246 www.mobio.com          ...
  • Page 14: Loading Sample Tubes

    Secure the finger plate in place over the Finger Plate   carousel and screw about 1/8 of a turn past where the fingers contact the top of the tubes. Do not over tighten! MO BIO Laboratories, Inc. 1-800-606-6246 www.mobio.com             13155 rev. 070110...
  • Page 15 4. Move the hub lock switch on the finger Hub  Lock  plate to the ‘Lock’ position. Switch  Close the door until the locking latch engages and locks. Tube Holder Finger Plate MO BIO Laboratories, Inc. 1-800-606-6246 www.mobio.com             13155 rev. 070110...
  • Page 16: Program Settings

    If the run is stopped or paused, the system status will read “RUN PAUSED.”  At the end of the program, “REMOVE SAMPLES” will be displayed followed by “PROGRAM DONE.” MO BIO Laboratories, Inc. 1-800-606-6246 www.mobio.com             13155 rev. 070110...
  • Page 17: Adjusting Parameters

    5,000 RPM in increments of 100 RPM. When the desired value is reached, the user can go to the next desired variable, or return to program ("PROG") to begin the run. CAUTION: To prevent tube breakage at high speeds, specialized bead tubes are required with speeds higher than 4,200 RPM. MO BIO Laboratories, Inc. 1-800-606-6246 www.mobio.com            ...
  • Page 18 The user can change the number of cycles using the  and  arrow keys. The number of cycles ranges from 1 to 10. When the desired value is reached, the user can go to the last variable, or return to program ("PROG") to begin the run. MO BIO Laboratories, Inc. 1-800-606-6246 www.mobio.com          ...
  • Page 19 24 Homogenizer immediately after the run has ended. ™ WARNING: The carousel can become very hot during operation. Use caution when handling the tube holder. *Dwell minimum varies depending upon the speed and length of cycle. MO BIO Laboratories, Inc. 1-800-606-6246 www.mobio.com             13155 rev. 070110...
  • Page 20: Recommended Settings

    2,000 – 1 x 5 min DNA isolation SOIL Soil (clay) 4,000 – 1 x 45 sec. – 0s Glass 0.1mm DNA isolation (loose, high humic) 2,500 – 1 x 45 sec. – 0s MO BIO Laboratories, Inc. 1-800-606-6246 www.mobio.com             13155 rev. 070110...
  • Page 21: Homogenizer Speed Conversion Table

    NOTE: Equivalent settings slower than 2,500 RPM or faster than 4,000 RPM on the PowerLyzer ™ 24 Homogenizer cannot be obtained when using the FastPrep or Precellys homogenizers. ® ® MO BIO Laboratories, Inc. 1-800-606-6246 www.mobio.com             13155 rev. 070110...
  • Page 22: Section 3. Troubleshooting

    24 Homogenizer in a manner other than that discussed ™ in this manual. For any issue that is unsuccessfully corrected using this guide, please contact Technical Support at MO BIO Laboratories, Inc. at 1-800-606-6246 or 760-929-9911. 3.1 ERROR MESSAGES Error Message...
  • Page 23: Section 4. Maintenance

    Pull out the fuse holder and inspect the fuse. If the core wire is burnt or broken, replace the fuse. Return each fuse holder to its original location. ON/OFF Switch Main Power Supply Plug Fuses 115V/230V Voltage Selection Switch MO BIO Laboratories, Inc. 1-800-606-6246 www.mobio.com             13155 rev. 070110...
  • Page 24: Cleaning And Decontamination

    10% bleach solution. Contact technical support if additional or alternative decontamination procedures are required to ensure the compatibility of the new procedure with the instrument. MO BIO Laboratories, Inc. 1-800-606-6246 www.mobio.com            ...
  • Page 25: Section 5. Transport And Storage

    ™   avoid damage which may invalidate the warranty. Packaging crates may be purchased for a minimal   charge. For additional repair and shipping inquiries, contact MO BIO at 1-800-606-6246.  MO BIO Laboratories, Inc. 1-800-606-6246 www.mobio.com             13155 rev. 070110...
  • Page 26: Storage

    WARNING: It is a violation of federal law to transport biologically hazardous or radioactive materials without proper packaging, labeling, and appropriate warnings. PowerLyzer is a trademark of MO BIO Laboratories, Inc. FastPrep is a registered trademark of MP Biomedicals, LLC. Precellys is a registered trademark of Bertin Technologies.
  • Page 27: Decontamination Certification Form

    BIO Laboratories decontamination testing it is found that the product has been tampered with and/ or contaminated, I understand that I will not receive a refund or a credit to my account. Version 07012010 MO BIO Laboratories, Inc. 1-800-606-6246 www.mobio.com          ...

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