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GelSpin™ DNA Purification Kit
UltraClean™ GelSpin Agarose Gel DNA Purification Kit
Introduction
Use this spin filter format kit for isolating DNA from agarose gels and cleaning up or purifying DNA from
reactions or solutions. You can isolate DNA from all types of agarose gels and buffer systems in 5
minutes. It is not necessary to use low melt agarose to get high yields. This kit will remove salts, buffers,
enzymes, ethidium bromide, label, and dNTPs from reactions. The resulting DNA can be used for any
downstream application.
Precautions
Please wear gloves when using this product. Avoid all skin contact with reagents in this kit. In case of
contact wash thoroughly with water. Do not ingest. See Material Safety Data Sheets for emergency
procedures in case of accidental ingestion or contact. All MSDS information is available upon request
(760-929-9911) or on our web site at www.mobio.com. Reagents labeled flammable should be kept away
from open flames and fire
This kit is for research purposes only. Not for diagnostic use.
Equipment required:
Microcentrifuge (13,000 x g)
Kit Contents: 250 purifications
Component
GelBind Buffer
GelWash
Elution Buffer
Spin Filter units
Collection tubes
Specifications:
DNA Size range: 100 bp - 50 kb
Types of agarose: All
Types of gel buffers: All
Spin Filter binding capacity: 20 µg
Final volume of DNA: 50 µl
Recovery rates: 80-100%
Kit Storage
Room temperature for 1 year.
Technical information: Toll free 1-800-606-6246, or 1-760-929-9911 email:
UltraClean™
Catalog # 12400-250
Instruction Manual
.
Amt.
Description
158 ml
NaClO
3 x 30 ml
Tris/ Ethanol solution
15 ml
10 mM Tris pH 8.0
250
Spin filter in centrifuge tube
250
2.0 ml centrifuge tubes
250 preps
solution
4
Page
1
006MB12400-250T
technical@mobio.com
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Related Manuals for MO BIO Laboratories UltraClean GelSpin DNA
Summary of Contents for MO BIO Laboratories UltraClean GelSpin DNA
- Page 1 Page 006MB12400-250T UltraClean™ GelSpin™ DNA Purification Kit Catalog # 12400-250 250 preps Instruction Manual UltraClean™ GelSpin Agarose Gel DNA Purification Kit Introduction Use this spin filter format kit for isolating DNA from agarose gels and cleaning up or purifying DNA from reactions or solutions.
- Page 2 Page 006MB12400-250T Detailed Protocol: (Explains what is occurring at each step) To isolate DNA from an Agarose gel 1. Cut desired DNA band from a TAE or TBE agarose gel. What’s happening: The agarose gel slice containing the desired DNA is removed from the rest of the gel. 2.
- Page 3 Page 006MB12400-250T To clean up DNA from a reaction solution. Introduction This process will clean up or purify DNA. It removes salts from reaction buffers, enzymes, label, dNTPs, ethidium bromide, and all other unwanted reaction components. 1. Add 3 volumes of GelBind to the DNA solution. For example, 0.1 ml (100µl) reaction requires 0.3 ml (300µl) of GelBind.
- Page 4 Page 006MB12400-250T Short Protocols: Please wear gloves All of the centrifugation steps are 10,000 x g. To isolate DNA from an Agarose gel 1. Cut desired DNA band from a TAE or TBE agarose gel. 2. Determine gel band weight. The following procedures should be carried out directly in the spin filter (for up to 0.2 gm of gel).
- Page 5 Page 006MB12400-250T Hints and Troubleshooting Guide Concentrating the DNA Your final volume will be 50µl. If this is too dilute for your purposes, add 2 µl of 5M NaCl and mix. Then add 100 µl of 100% cold ethanol. Mix. Centrifuge at 10,000 x g for 5 minutes. Decant all liquid.
- Page 6 Page 006MB12400-250T Other UltraClean™ Kits available from Mo Bio Laboratories, Inc. Kit description Cat. number Plasmid Prep Kits 6 minute Mini Plasmid Prep Kit (100 preps) 12300-100 6 minute Mini Plasmid Prep Kit (250 preps) 12300-250 25-50 ml Plasmid Prep Kit (20 preps)
- Page 7 Technical information: Call Mo Bio Laboratories, Inc. Toll free 1-800-606-6246, or 1-760-929-9911email technical@mobio.com Fax: 760-929-0109 Mail: Mo Bio Laboratories, Inc., 2746 Loker Avenue West, Carlsbad, CA 92008 Ordering Information Direct: Call Mo Bio Laboratories, Inc. Toll free 1-800-606-6246, or 1-760-929-9911 orders@mobio.com...
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