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PowerWater
(For isolation of genomic DNA from membrane filtered water samples)
Inhibitor Removal Technology
protection as well as international patents pending.
Please recycle
Technical Information: Toll free 1-800-606-6246, or 1-760-929-9911 Email:
®
Catalog No.
14900-50-NF
14900-100-NF
Instruction Manual
®
(IRT) is a registered trademark of MO BIO Laboratories, Inc. and is covered by US patent
DNA Isolation Kit
Quantity
Filters
50 Preps
No filters
100 Preps
No filters
technical@mobio.com
1
Version: 01052012
Website:
www.mobio.com
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Table of Contents
Related Manuals for MO BIO Laboratories PowerWater DNA
Summary of Contents for MO BIO Laboratories PowerWater DNA
- Page 1 No filters Instruction Manual ® Inhibitor Removal Technology (IRT) is a registered trademark of MO BIO Laboratories, Inc. and is covered by US patent protection as well as international patents pending. Please recycle Version: 01052012 Technical Information: Toll free 1-800-606-6246, or 1-760-929-9911 Email: technical@mobio.com...
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Page 2: Table Of Contents
Table of Contents Introduction ............................ 3 Protocol Overview........................... 3 Flow Chart ………........................... 4 Equipment Required ........................5 Kit Contents & Storage ........................5 Precautions & Warnings ........................ 5 Important Notes Before Starting...................... 5 Protocols: Experienced User Protocol....................6 Detailed Protocol (Describes what is happening at each step) ...........8 Vacuum Manifold Protocol ....................11 Hints &... -
Page 3: Introduction
After the protein and inhibitor removal steps, total genomic DNA is captured on the MO BIO Laboratories silica spin column. High quality DNA is then washed and eluted from the spin column membrane for use in downstream applications including PCR and qPCR. -
Page 4: Flow Chart
Technical Information: Toll free 1-800-606-6246, or 1-760-929-9911 Email: technical@mobio.com Website: www.mobio.com... -
Page 5: Equipment Required
Equipment Required Centrifuge for 15 ml tubes (≤4000 x g) Disposable/reusable filter funnels Filter membranes (if using a reusable filter funnel) Microcentrifuge (13,000 x g) Pipettors Vortex Vortex Adapter Vacuum Filtration System Kit Contents Kit Catalog# Kit Catalog# 14900-50-NF 14900-100-NF Component Amount Amount... -
Page 6: Experienced User Protocol
Disposable filter funnels, containing 0.22 µm or 0.45 µm filter membranes, can be ordered from MO BIO Laboratories (see page 3). The volume of water filtered will depend on the microbial load and turbidity of the water sample. (Please see Types of Water Samples in the Hints and Troubleshooting Guide section of the Instruction Manual). - Page 7 18. Shake to mix Solution PW4 before use. Add 650 µl of Solution PW4 and centrifuge at 13,000 x g for 1 minute. 19. Discard the flow through and add 650 µl of Solution PW5 and centrifuge at 13,000 x g for 1 minute.
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Page 8: Detailed Protocol (Describes What Is Happening At Each Step)
Disposable filter funnels, containing 0.22 µm or 0.45 µm filter membranes, can be ordered from MO BIO Laboratories (see page 3). The volume of water filtered will depend on the microbial load and turbidity of the water sample. (Please see Types of Water Samples in the “Hints and Troubleshooting Guide”... - Page 9 homogenization by holding the tubes equal distance and angle from the center of rotation. Avoid using tape, which can become loose and result in reduced homogenization efficiency. 8. Centrifuge the tubes ≤ 4000 x g for 1 minute at room temperature. The speed will depend on the capability of your centrifuge.
- Page 10 What’s happening: The DNA is selectively bound to the silica membrane in the Spin Filter basket and the flow through containing non-DNA components is discarded. 17. Place the Spin Filter basket into a clean 2 ml Collection Tube (provided). What’s happening: Due to the high concentration of salt in solution PW3, it is important to place the Spin Filter basket into a clean 2 ml Collection Tube to aid in the subsequent wash steps and improve the DNA purity and yield.
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Page 11: Vacuum Manifold Protocol
Vacuum Protocol using the PowerVac™ Manifold Please wear gloves at all times For each sample lysate, use one Spin Filter column. Keep the Spin Filter in the attached 2 ml Collection Tube and continue using the Collection Tube as a Spin Filter holder until needed for the Vacuum Manifold Protocol. - Page 12 9. Transfer the Spin Filter column to a new 2 ml Collection Tube and add 100 l of Solution PW6 to the center of the white filter membrane. Alternatively, sterile DNA-Free PCR Grade Water may be used for elution from the silica Spin Filter membrane at this step (MO BIO Catalog# 17000-10). 10.
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Page 13: Hints & Troubleshooting Guide
(0.22 micron). Use of 0.45 micron filters is recommended for theses types of samples. MO BIO Laboratories offers disposable filter funnels containing membranes of either 0.22 micron or 0.45 micron pore sizes (See page 3 for ordering information). - Page 14 Hints and Troubleshooting Guide cont. DNA Yield (ng/μl) Type of Water Sample Sample Volume Saltwater Bay 100 ml 40 - 72 Freshwater Lake 100 ml 15 - 25 Lagoon 20-100 ml 3 - 38 Ocean (coastal) 100 ml 3 - 11 Sewage influent 50 ml Treated effluent...
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Page 15: Contact Information
Phone MO BIO Laboratories, Inc. Toll Free 800-606-6246, or 760-929-9911 Email: technical@mobio.com Fax: 760-929-0109 Mail: MO BIO Laboratories, Inc, 2746 Loker Ave West, Carlsbad, CA 92010 Ordering Information: Direct: Phone MO BIO Laboratories, Inc. Toll Free 800-606-6246, or 760-929-9911 orders@mobio.com... -
Page 16: Other Quality Products Available
Other Quality Products Available from MO BIO Laboratories, Inc. For more product and detailed information go to www.mobio.com/catalog-request to request a catalog. RNA Isolation …Continued DNA Purification and Gel Extraction Catalog No. Quantity Catalog No. Quantity ® 12877-50 50 preps ®... - Page 17 Other Quality Products Available from MO BIO Laboratories, Inc. For more product and detailed information go to www.mobio.com/catalog-request to request a catalog. Genomic DNA Isolation Other Reagents and Lab …Continued Catalog No. Quantity Accessories Catalog No. Quantity ® 13096-4 4 x 96 preps...
- Page 18 Other Quality Products Available from MO BIO Laboratories, Inc. For more product and detailed information go to www.mobio.com/catalog-request to request a catalog. Other Reagents and Lab Instrumentation and Accessories…Continued Accessories…Continued Catalog No. Quantity Catalog No. Quantity KAPA2G FAST HotStart ReadyMix...
- Page 19 Other Quality Products Available from MO BIO Laboratories, Inc. For more product and detailed information go to www.mobio.com/catalog-request to request a catalog. Instrumentation and Accessories… Continued Catalog No. Quantity ® 13111-V 1 unit Vortex-Genie 2 Vortex (120V) 13111-V-220 1 unit Vortex-Genie ®...
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