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Summary of Contents for Turner BioSystems Picofluor
- Page 1 Picofluor™ Handheld Fluorometer Operating Manual Part Number: 998-0853 Version: 1.5...
- Page 2 Turner BioSystems, Inc. Picofluor™ is a trademark of Turner BioSystems, Inc. All of the trademarks, service marks, and logos used in these materials are the property of Turner BioSystems, Inc. or their respective owners.
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Page 3: Table Of Contents
Table of Contents Introduction ............................ 2 Description ............................. 2 General Information and Precautions ..............2 LCD ..............................2 Inspection ............................2 Setup ..............................2 Quick View Diagrams........................3 Instrument Workflow ........................4 Instrument Calibration and Operation ..................6 Instrument Power Up ........................6 LCD .............................. -
Page 4: Introduction
Use caution around solvents as they may corrode the plastic case of the Picofluor™. • If a liquid is accidentally spilled inside the Sample Compartment, invert the Picofluor™ to drain the excess liquid. Then wipe the inside area dry with a clean soft towel or tissue. -
Page 5: Quick View Diagrams
Quick View Diagrams Backlit Screen www.turnerbiosystems.com Version 1.5... -
Page 6: Instrument Workflow
Instrument Workflow Turner Designs TD HH1B 0500 5 5 second warm up XXX.X Indicates which channel Fluorescence reading is active Indicates activity of HOME Screen Press READ Press A/B to toggle Press CAL between Calib. READING XXX.X <ENT> to start Insert Blank WAIT XXX.X XXX.X... - Page 7 Press DATA Datalogger: <DATA> to toggle Press DATA 1X Press DATA 2X Press DATA 3X Status: Stop Download data: Erase Data <ENT> to toggle 5X<ENT> to start 5X<ENT> to start Press ENTER Status: Logging Download data: Erase Data <ENT> to toggle All downloaded All data erased If there is no data logged, this...
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Page 8: Instrument Calibration And Operation
Detection Channel Configuration The Picofluor™ has 2 fluorescence detection channels. Identify the configuration by looking at the label on the back side of the Picofluor™. See the table in Appendix A to confirm the correct configuration for your application. Select the appropriate channel by pressing the A/B button to toggle between the 2 channels. The display will show a label in the lower left corner of the HOME screen to identify which channel is activated. -
Page 9: Calibration
1. Press the CAL button. 2. Press the ENTER button to start the calibration. 3. Insert the blank sample and press the ENTER button. The Picofluor™ will average the reading for 10 seconds and set the blanking zero point. 4. Insert the standard sample and press the ENTER button. -
Page 10: Diagnostic Information
4. Instrument Calibration and Operation Diagnostic Information 1. Press the DIAG button to access the diagnostic screens. 2. The first screen shows the number of available data points for internal data logging. 3. Press the ENTER button to toggle to the RFU (Relative Fluorescence Unit) values from the calibration blank and standard. -
Page 11: Internal Data Logging (Idl)
Internal Data Logging (IDL) The Picofluor™ can log up to 1,000 data points. The DATA screens control logging, downloading, and erasing of data. To activate the IDL 1. Press the DATA button 2 times. 2. Press the ENTER button to toggle between logging and stopping. -
Page 12: General Considerations For Analysis
1. Take care not to spill liquid into the Sample Compartment. Promptly wipe any spills. 2. The Picofluor™ is very sensitive and even small amounts of material from a previous sample may result in errors. Use a clean cuvette for each reading. Thorough and proper cleaning of cuvettes between sample readings is essential, and is especially important if the same cuvette is used for both the sample and the blank. -
Page 13: Positioning Samples
Sample Compartment the same way each time to minimize error. Data Quality The Picofluor™ is only as accurate as the standards that are used to calibrate it. It is important to take care when preparing standards, samples, and blank. Follow the appropriate laboratory practices when preparing all solutions and reagents. -
Page 14: Warranty
Warranty Terms Turner BioSystems warrants the Picofluor™ Fluorometer and any accessories to be free from defects in materials and workmanship under normal use for a period of one year from the time of initial purchase under the following restrictions: •... -
Page 15: Out-Of-Warranty Service
Follow the same steps as for Warranty Service. Our Service Department is happy to assist you by telephone or correspondence at no charge. Repair service will be billed at a flat rate. Your invoice will include freight charges. Mailing Address: Turner BioSystems 645 North Mary Avenue Sunnyvale, CA 94085 Phone:... -
Page 16: Appendix A Instrument Specifications
APPENDIX A Instrument Specifications A.1 General Specifications Specification Description 1.75” x 3.5” x 7.25” Size (4.45 cm x 8.9 cm x 18.4 cm) Weight 14 oz (0.4 kg) Dynamic range 4 orders of magnitude Resolution 12 bits LCD Display 2 x 16 characters Case Meets IP 67 Standard;... -
Page 17: Appendix B Internal Datalogging
The boxes left of the words COM port and MS Excel should both be green. 6. Follow the directions from Section 5.2 for collecting and downloading data from the Picofluor™. Data will automatically appear in the Excel spreadsheet. Be sure to save this data before closing the software. - Page 18 Part Number: 998-0853 Version: 1.5 645 N. Mary Avenue • Sunnyvale, CA 94085 Phone: (408) 636-2400 •Toll Free: (888) 636.2401 • Fax: (408) 737-7919 www.turnerbiosystems.com.com...
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