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Related Manuals for Turner BioSystems GloRunner
Summary of Contents for Turner BioSystems GloRunner
- Page 1 GloRunner™ Microplate Luminometer Operating Manual Dated: 09/18/2006 Version: 1.2 Part Number: 998-9005 645 N. Mary Avenue Ÿ Sunnyvale CA 94085 Phone: 408.636.2400 Ÿ Toll Free: 888.636.2401 Fax: 408.737.7919 Ÿ www.turnerbiosystems.com www.turnerbiosystems.com...
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Page 2: Table Of Contents
Table of Contents I. Introduction Description Inspection General Precautions II. Hardware Overview Functions and Features III. Setting up the GloRunner™ Microplate Luminometer Procedure IV. GloRunner™ Microplate Luminometer DXL Software Overview Software Operation Communications Settings Procedure GloRunner™ User Interface Features Setting the Options V. - Page 3 APPENDIX C - Maintenance, Warranty, & Service Maintenance Warranty Obtaining Service Warranty Service Out-of-Warranty Service Address for Shipment: Error! Bookmark not defined. APPENDIX D – Specifications Specifications for the GloRunner™ Microplate Luminometer www.turnerbiosystems.com...
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Page 4: Introduction
Introduction Description The GloRunner™ is an easy-to-use, sensitive luminometer with a broad dynamic range designed to read glow luminescent reactions in 96-well plates. The instrument’s small size and affordable price make it an ideal laboratory instrument. This manual is designed to guide you through the installation, setup, calibration, and operation of your instrument. -
Page 5: Hardware Overview
Hardware Overview Functions and Features This section describes the various hardware features of the GloRunner™, with Figures 1-3 as references. v LED Status Lights: These lights report the status of the instrument. The green LED indicates the unit is on and ready. A solid yellow LED indicates the instrument is not communicating with the host computer. - Page 6 Figure 1 LED Status Lights Drawer Sample Tray Figure 2 9-pin Male Connector 9-pin Female Connector (To GloRunner™) (To Computer) RS-232 Serial Port Connection Power Power Cord Switch Connection Figure 3 www.turnerbiosystems.com...
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Page 7: Setting Up The Glorunner™ Microplate Luminometer
For added help while using the GloRunner™ DXL software, a tutorial is available. You will see the following dialog box after clicking on the GloRunner™ icon from your desktop (an alternate way of accessing the GloRunner™ DXL software is through the program manager): Figure 4 www.turnerbiosystems.com... - Page 8 If you elect to review the tutorial, you will see the following tutorial window. The tutorial can be printed by clicking the “Print” button. Figure 5 When you are done reviewing the tutorial, click the “Close” button to close the window.
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Page 9: Software Operation
Figure 6 Software Operation The GloRunner™ DXL software is designed to run in conjunction with Excel. When using the GloRunner™ Microplate Luminometer, you have two workbook options. The first option is to create a new workbook for data collection. This option should be selected when first using the GloRunner™. - Page 10 Note: These are two separate windows. Figure 8 The top window is the GloRunner™ dialog box. The bottom window is the active Excel spreadsheet in which data from the run will appear. The dialog box is used for starting runs, changing or selecting options, toggling the tray position, and establishing communication between the GloRunner™...
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Page 11: Communications Settings Procedure
Communications Settings Procedure 1. Select Communications from “Settings” on the GloRunner™ dialog box pull-down menu, and the screen in Figure 9 will be displayed. Figure 9 2. Select the proper COM port and click on “OK”. The COM port selection depends upon the computer that is connected to the instrument. - Page 12 3. Click on the “Tray In/Out” option, see Figure 11. If an error message appears, such as the one displayed in Figure 12, go back to step 2, and select the next COM port. For most computers, the COM port selection will need to be either COM 1 or COM 2.
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Page 13: Glorunner™ User Interface Features
1 second. An example can be seen in Figure 13. Figure 13 The status bar is another useful GloRunner™ feature. It indicates the status of the GloRunner™ during operation. For example, the following message is used to indicate that the GloRunner™ is getting ready to take readings: Figure 14 The GloRunner™... -
Page 14: Setting The Options
999-second delay between the end of one run and the start of the next run. 4. The GloRunner™ will automatically eject the microwell plate at the end of the run. If you prefer to temporarily store the plate within the instrument after the run, select the “Delay, before microplate ejection”... - Page 15 6. Select the wells you want to read. If the well is highlighted in blue, the well will be measured. Select or de-select all the wells by clicking the “All” button in the top left corner of the grid. Alternatively, individual rows and columns can be selected or de-selected by pressing the letter or number at the head of the row or column.
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Page 16: Reading Samples
5. Click on the “Start” button in the software to close the sample tray and start the instrument. 6. The GloRunner™ will read the whole plate in approximately 30 seconds if the measurement duration is set at 1 second. Once the run is complete, results will appear in the Excel spreadsheet. -
Page 17: Calibration Of The Glorunner
VI. Calibration of the GloRunner™ Overview Calibration of the GloRunner™ is possible if you purchased the GloRunner Standardization Light Plate. The calibration procedure is password protected and should only be done after contacting Turner BioSystems for the password. This calibration is a process that normalizes the detectors and is not usually required. - Page 18 3. Once “OK” is clicked the plate will be drawn into the GloRunner™ and the detectors will be standardized. 4. If you see a message similar to Figure 23, and your light plate is powered on, please contact Turner BioSystems for support: Figure 23 www.turnerbiosystems.com...
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Page 19: Relative Light Units And Common Questions
A: For weakly luminescent samples, white plates give better results because they reflect more light to the instrument’s detector. Q: What luminescent assays can I run on the GloRunner™ Microplate Luminometer? A: The instrument is designed for glow luminescent reactions, so any chemistry that has a half-life of 3 minutes or greater is appropriate. - Page 20 Q: What is the purpose of the Dark Current Log? A: The Dark Current is a measurement the detectors make in a position on the instrument that represents total darkness. These measurements are logged for technical support purposes. Logging these values can be valuable in diagnosing instrument related performance issues.
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Page 21: Appendix A - Installing The Software
95 or later. To install the software, follow the instructions below: Procedure 1. Place the CD disk of the Turner BioSystems GloRunner™ Microplate Luminometer software in the D: drive of your computer. Most computers will automatically start the installation process once the disk is placed in the CD-ROM. - Page 22 3. Follow the instructions given during the installation process. The default directory for installation is C:\Program Files\Turner BioSystems\GloRunner. To select an alternative directory, click on the “Change” button shown in Figure 25 and choose your directory option as seen in Figure 26. Click the “OK” button when you are ready to continue with the software installation.
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Page 23: Appendix B - Led Messages And Firmware Upgrades
APPENDIX B - LED Messages and Firmware Upgrades LED Lights There are 3 LED status lights on the front panel of the GloRunner™. v The green LED is the power indicator. When it is illuminated the instrument is powered and ready. -
Page 24: Appendix C - Maintenance, Warranty, & Service
Warranty Turner BioSystems warrants the GloRunner™ Microplate Luminometer and accessories to be free from defects in materials and workmanship under normal use and service for a period of one year from the time of initial purchase, with... -
Page 25: Obtaining Service
1. To obtain service during the warranty period, please take the following steps: Write or call the Turner BioSystems Service Department and describe as precisely as possible the nature of the problem. 2. Carry out minor adjustments or tests as suggested by the Service Department. -
Page 26: Out-Of-Warranty Service
Repair service will be billed on a flat rate basis. Your invoice will include freight charges. Address for Shipment: Turner BioSystems 645 N. Mary Ave. Sunnyvale, CA 94085 Telephone: 408-636-2400 Toll-Free: 888-636-2401 (US & Canada) Fax: 408-737-7919 Email: techsupport@turnerbiosystems.com... -
Page 27: Appendix D - Specifications
APPENDIX D – Specifications Specifications for the GloRunner™ Microplate Luminometer 2x10 moles horseradish peroxidase Sensitivity 2x10 moles alkaline phosphatase 5x10 moles luciferase Instrument dynamic range 10 Linear Dynamic Range Better than 5x10 at 1.5x10 Relative Light Units using Cross-talk a white Costar plate...
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