Related Manuals for Turner BioSystems TBS-380
Summary of Contents for Turner BioSystems TBS-380
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Page 1: Operating Manual
User’s Manual TBS-380 Mini-Fluorometer Operating Manual Dated: 08/14/2007 Rev. E 645 N. Mary Avenue • Sunnyvale, CA 94085 Phone: (408) 636-2400 • Toll Free: (888) 636.2401 • Fax: (408) 737-7919 www.turnerbiosystems.com... - Page 2 For the most current version of this manual, visit our Customer Care Center at www.turnerbiosystems.com...
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Page 3: Table Of Contents
Table of Contents Introduction 1.1. Description ..............................3 1.2. Unpacking and Inspection .......................... 3 1.3. General Information and Precautions ......................3 Quick View Diagram ............................4 Instrument Parameters 3.1. Setup Flowchart ............................6 3.2. Instrument Power Up ..........................8 3.3. Fluorescence Channels ..........................8 3.4. - Page 4 User Notes and Information Date Notes/Information...
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Page 5: Introduction
2. Assemble the power supply: choose the proper AC clip for your outlet and slide it on to the AC adaptor. 3. Plug the power supply into the TBS-380 (see Section 2. Quick View Diagram). Be sure to use only the power supply provided with your instrument by Turner BioSystems. Powering the instrument with an unapproved power supply may damage the unit. -
Page 6: Quick View Diagram
2 Quick View Diagram Sample 10x10 mm Cuvette Holders Compartment LCD Screen Minicell Cuvette Holders ON/OFF button Power Supply Input Serial Port Positioning the Minicell Adaptor BLUE Position UV Position TBS-380 Mini-Fluorometer Operating Manual... - Page 7 UP ARROW button DOWN ARROW STD VAL button. button. Press to set numeric value for the standard solution. ENTER button. DATA button. Press to access IDL screens (Internal Data Logging.) DIAG button. Press to access DIAGNOSTICS screens. TBS-380 Mini-Fluorometer Operating Manual...
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Page 8: Instrument Parameters
Press ESC button to return 10X or examine the blank for high from previously recorded value by 5% or fluorescence background. to the HOME screen more. If it does, recalibrate the instrument. If it does not, record the reading. TBS-380 Mini-Fluorometer Operating Manual... - Page 9 XXX.X XXX.X Press READ button CONT: count = XX <ENT> int = X sec Press ENTER button Insert Sample Press <ENTER> Insert sample and press ENTER button READING #X WAITING #X XX.X XXX.X TBS-380 Mini-Fluorometer Operating Manual...
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Page 10: Instrument Power Up
Press the UP or DOWN ARROW button to abort or accept the calibration, respectively. If desired, press the ESC button at any time during steps 1-4 to stop the calibration. The TBS-380 will return to the HOME screen and default back to the previous calibration. -
Page 11: Sample Analysis
11. Insert your sample and Press the ENTER button again. 12. Once all counts are measured, the result will be displayed at the top center of the HOME screen. 13. To abort the continuous sample measuring, press the ESC button and then the ENTER button. TBS-380 Mini-Fluorometer Operating Manual... -
Page 12: Internal Data Logging (Idl)
Internal Data Logging (IDL) The TBS-380 can log up to 1000 data points to the internal memory. The DATA screen controls logging, downloading and erasing of data. 3.7.1 Activating Data Logging 1. Press the DATA button. 2. Press the DATA button. -
Page 13: General Considerations For Analysis
1. Be cautious not to spill samples into the sample chamber. Promptly wipe any spills. 2. The TBS-380 is very sensitive and even small amounts of material left behind from a previous sample may result in errors. Use a clean cuvette or disposable cuvette for each reading. -
Page 14: Temperature Considerations
Data Quality The TBS-380 is only as accurate as the standards that are used to calibrate it. It is important to follow correct laboratory practices when preparing all solutions and reagents including standards, samples, and blank. -
Page 15: Troubleshooting
The minimum volume is 2 mL for 10x10 mm cuvette, 50 µL for the Minicell. Check that the reagents and cuvette material are compatible. Check temperature (see 4.3). Confirm that the RFU-STD is greater than RFU-Blk by factor of ≥ 3. TBS-380 Mini-Fluorometer Operating Manual... -
Page 16: Terms
6 Warranty Terms Turner BioSystems warrants the TBS-380 Mini Fluorometer and any accessories to be free from defects in materials and workmanship under normal use for a period of one year from the time of initial purchase under the following restrictions: •... -
Page 17: Out-Of-Warranty Service
Repair service will be billed on a basis of time and materials. A complete statement of time spent and materials used will be supplied. Shipment to Turner BioSystems should be prepaid. Your bill will include return shipment freight charges. -
Page 18: Instrument Specifications
High blank Cuvette Type 10x10 mm plastic, 10x10 mm Minicell Adaptor Warm Up Time 5 seconds Optical Specifications Light Source UV LED Blue LED Excitation Optics 365–395 nm 465–485 nm Emission Optics 440–470 nm 515–575 nm TBS-380 Mini-Fluorometer Operating Manual... -
Page 19: Internal Data Logging
COM port and MS Excel should both be green. 6. Follow the directions from Section 3.7 for collecting and downloading data from the TBS-380. Data will automatically appear in the MS Excel spreadsheet. Be sure to save this data before closing the software. - Page 20 645 N. Mary Avenue • Sunnyvale, CA 94085 Phone: (408) 636-2400 • Toll Free: (888) 636.2401 • Fax: (408) 737-7919 www.turnerbiosystems.com...
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