Table of Contents
Advertisement
Quick Links
Advertisement
Chapters
Table of Contents
Related Manuals for Amersham Biosciences AKTAexplorer
Summary of Contents for Amersham Biosciences AKTAexplorer
- Page 1 ÄKTA explorer Installation Guide 18-1139-59...
- Page 3 Unless otherwise agreed in writing, all goods and services are sold subject to the terms and conditions of sale of the company within the Amersham Biosciences group which supplies them. A copy of these terms and conditions is available on request.
-
Page 5: Table Of Contents
Contents Contents 1 About this installation guide ......7 2 Safety ............8 3 Pre-requisites ..........10 4 Installation overview ........11 5 Installation of ÄKTAexplorer ......12 5.1 Unpacking ................. 12 5.2 Installing the pH flow cell ............13 5.3 Installing column holders and tubing holders ...... - Page 6 Contents ÄKTAexplorer Installation Guide 18-1139-59 Edition AC...
-
Page 7: About This Installation Guide
About this installation guide 1 About this installation guide ™ ÄKTAexplorer is assembled and fully tested before shipping. For safe transportation, however, some components have been detached and thus need to be remounted. Cables, capillaries, accessories, column holder, etc. are enclosed in paper boxes or in Box-900 located at the top of the instrument pile. -
Page 8: Safety
Safety 2 Safety • The system is designed for indoor use only. • Do not use in a dusty atmosphere or close to spraying water. Refer to Technical Specifications in the System Manual for detailed environmental pre-requisites. WARNING! The individual instruments must not be opened by the user. - Page 9 Safety WARNING! If the system is turned around or the fraction collector removed, the external capillaries and other tubings may become entangled in nearby objects and be pulled from their connections causing leakage. WARNING! Never place waste containers on the top of the system. If they become full and overflow, liquid may penetrate the system causing a short-circuit.
-
Page 10: Pre-Requisites
Pre-requisites 3 Pre-requisites WARNING! ÄKTAexplorer must be connected to a grounded mains socket. • Two people are required to lift ÄKTAexplorer onto the working bench. • To install ÄKTAexplorer, a working area of about 200 x 80 cm is required. •... -
Page 11: Installation Overview
Installation overview 4 Installation overview • Unpack ÄKTAexplorer ............12 • Detach packing material, and install items enclosed....12 • Unpack and install the computer..........16 • Connect mains power cabling ..........17 • Connect UniNet-1 data communication chain cabling .....16 • Complete the first two sections of the installation record..30 •... -
Page 12: Installation Of Äktaexplorer
Installation of ÄKTAexplorer 5 Installation of ÄKTAexplorer Begin by creating a clean and dry working area of 200 x 80 cm that allows easy access. Then follow the step-by-step instructions below and fill in the installation record as you go along, see page 30. Note: Some components are packed in Box-900, located at the top of the system. -
Page 13: Installing The Ph Flow Cell
Installation of ÄKTAexplorer 5.2 Installing the pH flow cell Install the pH flow cell holder (packed in Box-900) in the groove directly below the outlet valve (V4) on the outside of the valve door. Push the slide clamp upwards to secure the pH flow cell holder. Mo nit or P9 M on... -
Page 14: Installing Column Holders And Tubing Holders
Installation of ÄKTAexplorer 5.3 Installing column holders and tubing holders Mo nito r P9 10 M on ito r P9 10 n ito Install the column holders (17a, 17b packed in Box-900) onto the valve door. They should be placed close to the column selection valves (V2 and V3). -
Page 15: Connecting Tubings
Installation of ÄKTAexplorer Mo nito r P9 10 M on ito r P9 10 n ito Locate the sample pump waste tubing (23) penetrating the valve door. Remove the wire clips (22) from the tubing and place the outlet in an E-flask (24) placed on the sample tray. Note: For calibration of the sample pump, refer to the Pump P-960 User Manual. -
Page 16: Installing The Computer And The Mains Cable
Installation of ÄKTAexplorer Snap the inlet tubings A11, A2, B1 and B2 into the tubing holder (26) located at the upper left. Mo nit or P9 M on ito r P 91 n it Turn ÄKTAexplorer on its swivel platform to access the valve door. Remove the wire clips from the waste tubing connected to port 5 (29) on the injection valve (V1). -
Page 17: Connect Mains Power Cabling
Installation of ÄKTAexplorer CAUTION! The UniNet connection to the computer MUST be made to the board with four LEDs (37). If using a fraction collector, connect UniNet 1 cable (35) between Pump P-900 and the fraction collector, and UniNet 1 cable (36) between the fraction collector and the computer. -
Page 18: Installation Test
Installation test 6 Installation test The installation test is designed to check the function of the liquid delivery and the UV monitoring system of ÄKTAexplorer. The installation test can also be used at any time to check the condition of the system, e.g. -
Page 19: Priming And Purging Pump P-900
Installation test Click the button in the taskbar. System Control System Control button 6.2 Priming and purging Pump P-900 Prime the piston seal rinsing system of pump P-900 as follows: Immerse the rinsing tubing in a flask (1) containing 20% ethanol in distilled water. - Page 20 Installation test 6.2.1 Testing pressure stability Perform a pressure test to establish that all air has disappeared from the pump heads. Perform as follows: Connect a column bypass capillary between the injection valve, port 1, and the top of the UV optical unit. ÄKTAexplorer 10 (to check pump module A): Run 0.2 ml/min at 0%B (distilled water).
-
Page 21: Preparing The Installation Test
Installation test 6.3 Preparing the installation test Move inlet tubing B1 to a flask containing 500 ml of 0.4% acetone in distilled water. Make sure that a bypass capillary is installed between port 1 of the V2 and V3 column selection valves. In the module, select menu . -
Page 22: Running The Installation Test Method
Installation test 6.4 Running the installation test method , select . Select the UNICORN Main menu File:Printer Setup... appropriate printer from the list and select . Then click Landscape to acknowledge the printer chosen. Click the button . The window opens. Instant Run Instant Run Select the appropriate system and click... - Page 23 Installation test Select in the Method Wizard. Click Installation_Test In the window, select the procedure for your Evaluation Procedures system, for example, InstTest_Explorer100 Note: Explorer 10 systems have a specfic evaluation procedure that includes the autosampler peaks. The name of the procedure has the extension _A90X.
- Page 24 Installation test Click the tab. Curves Select the following curves to be displayed: • UV1_265nm • UV2_254nm • UV3_280nm • Conc. Clear all other highlighted curves. Click ÄKTAexplorer Installation Guide 18-1139-59 Edition AC...
-
Page 25: Evaluating The Installation Test Results
Installation test 10 When the test is finished, the printer automatically prints the chromatogram and the test result. 100% Step response result Gradient result 6.5 Evaluating the installation test results 6.5.1 Automatic evaluation The system automatically prints the test result when the test is finished. The print-out consists of a chromatogram and an evaluation of the test result. - Page 26 Installation test 6.5.2 Manual evaluation If your chromatography system deviate from the standard configuration, e.g. if optional components have been installed, the automatic evaluation will not give a reliable result. If so, perform a manual evaluation as described below. Select the module.
-
Page 27: Evaluate The Gradient
Installation test Evaluating the gradient Place a ruler along the gradient part of curve in the printed report. Wizard Generated001:1_UV1_265nm@01,SMTH The curve should be linear between 10% B and 90% B and void of discontinuities. Evaluating the step response Calculate the relative adsorption plateau heights for curve as follows: Wizard Generated001:1_UV1_265nm@01,SMTH Subtract the base line value (0%B) from each of the values in... -
Page 28: Correcting Faulty Evaluation Results
Installation test 6.6 Correcting faulty evaluation results Should any of the evaluated values fall outside the specified range, proceed as follows: • If the system differs from the standard configuration, evaluate the result manually. If the faulty evaluation result remains, continue below. 6.6.1 Faulty gradient •... -
Page 29: Test Record
Test record 7 Test record Date: ................ÄKTAexplorer serial no.: ..........7.1 Gradient test result Gradient linear from ....%B to ....%B. (10 - 90%) 7.2 Step response test result Step response table: Programmed Value Baseline cor- Normalised Allowed Conc.%B read rected value value... -
Page 30: Installation Record
Installation record 8 Installation record Check Sign Remarks 1 Unpacking • Contents according to packing lists. • All packing material removed. • No visible damage. 2 Installation • Injection valve waste tubings (port 4 and 5, marked W1 and W2) to waste reservoir. -
Page 31: Registration Form
Registration form 9 Registration form IMPORTANT! WARRANTY REGISTRATION INFORMATION Please ensure that this form is completed and returned to Service Administration to register the users' equipment under warranty. Name: ..........................Institute/company: ......................Address: ........................... Department/location: ...................... Post Code: ........................Phone Number: ..........Fax Number: ........End Users:............ -
Page 32: Components
Registration form 9.1 Components ÄKTAexplorer system serial numbers: ..........Part Number Description Serial Number System rack Mixer M-925 Monitor UV-900 Pump P-900 pH/C-900 INV-907 PV-908 SV-903 (A) SV-903 (B) Computer Computer display ÄKTAexplorer Installation Guide 18-1139-59 Edition AC... - Page 33 Registration form ÄKTAexplorer Installation Guide 18-1139-59 Edition AC...
- Page 34 Registration form ÄKTAexplorer Installation Guide 18-1139-59 Edition AC...
Need help?
Do you have a question about the AKTAexplorer and is the answer not in the manual?
Questions and answers