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Summary of Contents for Amersham Biosciences MegaBACE 500
- Page 1 Artisan Technology Group is your source for quality new and certified-used/pre-owned equipment SERVICE CENTER REPAIRS WE BUY USED EQUIPMENT • FAST SHIPPING AND DELIVERY Experienced engineers and technicians on staff Sell your excess, underutilized, and idle used equipment at our full-service, in-house repair center We also offer credit for buy-backs and trade-ins •...
- Page 2 MegaBACE 500 and 1000 Planning Guide Revision 6 December 2002 256-003 63004918 (SV# 399-326) Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
- Page 3 All goods and services are sold subject to the terms and conditions of sale of the company within the Amersham Biosciences group that supplies them. A copy of these terms and conditions is available on request.
- Page 4 PE Applied Biosystems, 850 Lincoln Center Drive, Foster City, California 94404. PE Applied Biosystems does not guarantee the performance of this instrument. Amersham Biosciences Limited is a licensed vendor for authorized reagents. The MegaBACE SNuPe genotyping kit is supplied under license from Orchid BioSciences Inc and is covered by US patent numbers 5,888,819;...
- Page 5 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
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Page 6: Table Of Contents
Installing a nitrogen cylinder ......2-10 MegaBACE 500 and 1000 Planning Guide Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com... - Page 7 Preinstallation checklist ........A-2 Appendix B Training checklist Appendix C Summary of laboratory space requirements MegaBACE 500 and 1000 Planning Guide Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
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Page 8: Preface
Preface About this guide The MegaBACE 500 and 1000 Planning Guide describes how to plan and prepare your laboratory before you receive the MegaBACE™ DNA Analysis System. This guide describes the requirements for the MegaBACE 500, flexible MegaBACE 1000, and MegaBACE 1000 instruments. -
Page 9: Safety Standards
Tel: +81 3 5331 9336 Tel: +1 800 526 3593 Fax: +81 3 5331 9370 Fax: +1 877 295 8102 Web site http://www.amershambiosciences.com viii MegaBACE 500 and 1000 Planning Guide Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com... -
Page 10: Chapter 1 Introduction
Uninterruptible fan module power supply* Laboratory bench* Nitrogen cylinders* * Not provided with the system. Figure 1-1. The MegaBACE system. MegaBACE 500 and 1000 Planning Guide p1-1 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com... -
Page 11: Preinstallation Tasks
Other components (user documents and software CD) • Installation and training • One-year warranty • Liability statement for the MegaBACE system • (Optional) Service contract p1-2 MegaBACE 500 and 1000 Planning Guide Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com... -
Page 12: Additional Recommended Components
See chapter 4 for recommended configurations. Contact MegaBACE System Technical Support for specifications. See Assistance in the preface for contact information. MegaBACE 500 and 1000 Planning Guide p1-3 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com... - Page 13 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
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Page 14: Chapter 2 Site Requirements
Space for an uninterruptible power supply (UPS) if you plan to use one See figure 2-1 for details on space requirements. MegaBACE 500 and 1000 Planning Guide p2-1 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com... - Page 15 2. In addition to the components shown, the use of a UPS is recommended. 3. Computer and monitor dimensions can vary depending on the model provided. Figure 2-1. The MegaBACE system space requirements. p2-2 MegaBACE 500 and 1000 Planning Guide Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
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Page 16: Laboratory Bench Requirements
Installation category: Place the MegaBACE instrument away from heat and air conditioning ducts. Do not place the instrument in direct sunlight. MegaBACE 500 and 1000 Planning Guide p2-3 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com... -
Page 17: Heat Production
Important You must locate the right side of the MegaBACE instrument within 2.5 m (8 ft) of the electrical outlets. p2-4 MegaBACE 500 and 1000 Planning Guide Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com... -
Page 18: Uninterruptible Power Supply (Ups)
finish a run that is in process and allow the capillaries to be stored properly. Amersham Biosciences recommends the use of a UPS rated for at least 4 kVA. Using a UPS protects the instrument, the capillaries, and your data from damage or loss caused by unexpected power failures, surges, or AC line fluctuations. -
Page 19: Instrument And Power Supply Fan Module: Europe
, using the standard country-accepted outlet. Alternatively, you can use a single grounded circuit capable of delivering at least 20 A at 230 V p2-6 MegaBACE 500 and 1000 Planning Guide Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com... -
Page 20: Computer And Monitor
Use only hose types with ratings that exceed the required operating pressures. Do not use a frayed or damaged hose, which can rupture and can cause injury. MegaBACE 500 and 1000 Planning Guide p2-7 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com... -
Page 21: Ordering Information For The High- And Low-Pressure Nitrogen Systems
(408) 617-2800 (Rev. F) (or check with your local nitrogen supplier) Note: The female adapter comes in the instrument accessory kit. p2-8 MegaBACE 500 and 1000 Planning Guide Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com... -
Page 22: Requirements For The High-Pressure Nitrogen System
Low setpoint—6.55 × 10 kPa (950 psi) • Volume used (estimated)— Single run—0.0006 m (0.02 ft Five runs per day—0.003 m (0.1 ft MegaBACE 500 and 1000 Planning Guide p2-9 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com... -
Page 23: Requirements For The Low-Pressure Nitrogen System
(1 000 psi) • The low-pressure regulator gauge is set to the proper pressure: 6.89 × 10 kPa (100 psi) p2-10 MegaBACE 500 and 1000 Planning Guide Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com... -
Page 24: Chapter 3 Emission Filters, Chemistry, And Laboratory Materials
3-1 and 3-2. The same set of beamsplitters and filters can be used to detect the SNuPe™ dye set (table 3-3) for SNP genotyping. MegaBACE 500 and 1000 Planning Guide p3-1 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com... - Page 25 Beamsplitters and filters for the SNuPe dye set SNuPe Spectral SNuPe dye base order channel Filter Beamsplitter 555DF20 A: 540DRLP R110 520DF20 610LP B: 595DRLP TAMRA 585DF20 p3-2 MegaBACE 500 and 1000 Planning Guide Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
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Page 26: Filter Sets For Str Genotyping
Dye set 1 Color channel Filter Beamsplitter ET-ROX 610DF20 A: 540DRLP Blue 520DF20 Yellow or black 3 565DF20 B: 555DRLP Green 545DF20 MegaBACE 500 and 1000 Planning Guide p3-3 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com... -
Page 27: Chemistry
25 µg 27-4951-01 * You can order the reagents from your local Amersham Biosciences sales office. For a complete listing, see the Web site: http://www.amershambiosciences.com. † Choose the blend that is appropriate for your application and conditions. p3-4 MegaBACE 500 and 1000 Planning Guide... - Page 28 2 plates Amersham Biosciences 25-0207-01 test plate kit http://www.amershambiosciences.com * You can order the reagents from your local Amersham Biosciences sales office. For a complete listing, see the Web site: http://www.amershambiosciences.com. Table 3-8. Chemistry requirements for SNP genotyping Item Storage conditions...
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Page 29: Laboratory Equipment
Pyrex™ or equivalent glass dish measuring 20.32 cm by 20.32 cm by 5.08 cm (8 in by 8 in by 2 in) (recommended for dialysis procedure) p3-6 MegaBACE 500 and 1000 Planning Guide Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com... -
Page 30: Consumables
(lubricant for anode plug O-ring) http://www.coleparmer.com Within US: (1) (800) 323-4340 Outside US: (847) 549-7600 or refer to Web site MegaBACE 500 and 1000 Planning Guide p3-7 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com... - Page 31 Web site • Tape pads Qiagen 19570 (for 4 °C storage and vortexing) http://www.qiagen.com or within US: (1) (800) 426-8157 p3-8 MegaBACE 500 and 1000 Planning Guide Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
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Page 32: Additional Consumables Required For Str Genotyping
Various methods exist for desalting the samples, including dialysis, ethanol precipitation, and the use of Sephadex™ separation resins or magnetic beads. Amersham Biosciences recommends dialysis, but alternative methods can also produce good results. Table 3-10 lists the consumables required for dialysis. -
Page 33: Additional Consumables Required For Snp Genotyping
27-5340-96 for site listing) AutoSeq96 adapter plate Amersham Biosciences 27-5341-01 (for use with non-skirted plates) (see http://www.amershambiosciences.com for site listing) p3-10 MegaBACE 500 and 1000 Planning Guide Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com... -
Page 34: Chapter 4 Planning For Computer Network And Data Storage
Fast Ethernet Adapter so that it can be connected to a supported network. Call MegaBACE System Technical Support for information on the exact computer configuration. MegaBACE 500 and 1000 Planning Guide p4-1 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com... -
Page 35: Configuration For A High-Throughput Laboratory
150 MB of disk space. You should plan to archive or back up the large files to meet your requirements. p4-2 MegaBACE 500 and 1000 Planning Guide Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com... -
Page 36: Chapter 5 Moving And Reinstalling The Instrument
Required parts for reinstalling the instrument (section 5.2) • Connecting a new computer (section 5.3) An Amersham Biosciences representative installs your MegaBACE instrument after delivery. If you need to move the instrument, contact your Amersham Biosciences representative before you move it. -
Page 37: Major Components And Parts
See chapter 4 for networking configurations. Install the MegaBACE Instrument Control Manager and analysis software using the instructions provided with the software. p5-2 MegaBACE 500 and 1000 Planning Guide Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com... -
Page 38: Appendix A Preinstallation Forms
Preinstallation checklist (section A.2) A.1 Site information form Fill in the site and system information in the spaces provided below. You and the Amersham Biosciences MegaBACE representative will need this information to schedule your MegaBACE system installation. Organization name: User/manager name(s):... -
Page 39: Preinstallation Checklist
(STR genotyping only) Provide 4 °C storage for the size standard and spectral matrix standards. pA-2 MegaBACE 500 and 1000 Planning Guide Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com... - Page 40 Sealing tape for the filtration plate Pyrex or equivalent glass dish SNP genotyping only Consumables for PCR cleanup Consumables for cleanup of SNuPE reaction MegaBACE 500 and 1000 Planning Guide pA-3 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
- Page 41 SNuPe multiple injection marker kit SNuPe control kit * Choose the blend that is appropriate for your application and conditions. pA-4 MegaBACE 500 and 1000 Planning Guide Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
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Page 42: Appendix B Training Checklist
Appendix B Training checklist The checklist below provides a list of items that should be covered when training the MegaBACE system users. You and the Amersham Biosciences MegaBACE representative will determine which tasks to assign to each MegaBACE system user. - Page 43 Maintenance and troubleshooting System specifications Cleaning the cathode and anode Cleaning the air filter Cleaning the water tanks and bottles pB-2 MegaBACE 500 and 1000 Planning Guide Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
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Page 44: Appendix C Summary Of Laboratory Space Requirements
* The power supply fan module and the computer tower can be located below the laboratory bench. The UPS, if used, can be located on the floor near the laboratory bench. † Computer and monitor dimensions can vary depending on the model provided. MegaBACE 500 and 1000 Planning Guide pC-1... - Page 45 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
- Page 46 STR emission filter configurations ordering information sequencing emission filter configurations ordering information SNP genotyping emission filter configurations ordering information MegaBACE 500 and 1000 Planning Guide Index-1 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
- Page 47 2-10 humidity requirements leak testing 2-10 regulator configuration specifications installation system ordering information cautions warning nitrogen cylinder 2-10 noise reduction Index-2 MegaBACE 500 and 1000 Planning Guide Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
- Page 48 2-10 SNP genotyping. see genotyping, SNP space requirements details dimensions summary temperature requirements terminators, ET Thermo Sequenase training checklist MegaBACE 500 and 1000 Planning Guide Index-3 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
- Page 49 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
- Page 50 Artisan Technology Group is your source for quality new and certified-used/pre-owned equipment SERVICE CENTER REPAIRS WE BUY USED EQUIPMENT • FAST SHIPPING AND DELIVERY Experienced engineers and technicians on staff Sell your excess, underutilized, and idle used equipment at our full-service, in-house repair center We also offer credit for buy-backs and trade-ins •...
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