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Main power switch: Turns the power to the unit On/Off. Illuminates green when On.
Temperature controller and temperature set: The controller has a 3-digit display for displaying chamber temperature or setpoint
information. Up and Down arrows are used to change the setpoint and controller mode of operation. To enter the setpoint mode
of operation, press either the Up or Down arrows one time. The display will start to blink, going from bright to dim and will show
the setpoint. To change the setpoint, use the Up and Down arrows. If the arrows are not pressed for five (5) seconds, the display
will stop blinking and will read the chamber temperature. After setting the temperature, allow at least one hour for the chamber
temperature to stabilize and 24 hours for optimum stabilization.
Calibration: The Labnet ProBlot hybridization oven is calibrated at 65°C at the factory. The unit can be recalibrated after the
chamber temperature has stabilized at the setpoint for several hours. Suspend a certified reference thermometer in a hybridization
bottle such that it does not touch the glass bottle sides, or (less accurate) tape the reference thermometer to the outside wall of a
hybridization bottle. Mount the bottle on the rotisserie (and a second bottle for balance), and run the unit at the desired temperature
for 2 hours. Compare the units display to the reference thermometer. If there is an unacceptable difference, put the controller into
calibration mode by pressing both the Up and Down arrows at the same time until the two outside decimal points begin to flash. While
the decimal points are flashing, the display can be calibrated to match the reference thermometer by pressing the Up or Down arrows
until the display reads the correct value. Allow the oven to stabilize again, and recalibrate if necessary.
Heating Indicator: Illuminates green when the controller is calling for heat from the heater. This indicator will be On continuously
while the oven heats up to the set temperature and will then cycle On and Off at the set temperature.
Rotisserie power switch: Turns On the power to the rotisserie, and is used to start and stop the rotisserie.
Rotisserie speed knob: Controls the rotisserie speed. Clockwise rotation increases speed. The oven has a nominal speed range of
4 to 20 rpm.
8.0 Rotisserie
The rotisserie is stainless steel and has clips to hold hybridization bottles. Always load the rotisserie with an even number of bottles
in a balanced pattern. The standard rotisserie will hold either six or twelve large (300 mm) bottles and double the number of small
(150 mm) bottles. The clips may be squeezed inward to also hold 50 mL tubes. Optional rotisseries that hold other styles of bottles or
vertical 50 mL tubes are available.
8.1 Setting Rotisserie Offset Angle
The rotisserie can be adjusted to hold bottles either horizontally or at a slight angle for more vigorous wetting action. To adjust the
angle, first loosen the screws on one hub then rotate the hub to create an angle offset between the two hubs, then retighten the screws.
Additional angle can be obtained by loosening the screws and rotating the second hub in the opposite direction from the first hub.
8.2 Rotisserie Removal and Installation
CAUTION: Rotisserie and oven sides may be hot.
Bottles may need to be removed before attempting to remove or install the rotisserie. The rotisserie is held and driven by a two-prong
fork drive on one side and locked in a cradle on the other side by a brass locking pin. To remove the rotisserie, first remove the brass
locking pin, then lift the rotisserie up from the cradle while simultaneously moving the rotisserie away from and off the two-prong
fork drive. Rotisserie installation is the reverse.
9.0 Rocking Platform Installation
An optional rocker platform is available for the Labnet ProBlot™ hybridization oven. Before installing the rocker platform, the rotisserie
must first be removed (see Section 8.2). To install the rocking platform, first install the fork adapter (rectangular bar with holes) on
the rotisserie drive fork by pressing the adapter over the two prongs and tighten the Allen screw in the end of the adapter with the
wrench provided. Do not overtighten.
Next, use the rotisserie speed control to bring the fork drive and adapter to a horizontal position.
Place the rocking platform in the oven and connect the platform drive arm to the fork drive adapter using the thumbscrew. Two
positions are available on the adapter. Select the position that allows the platform to sit horizontal (or close to horizontal) when the
fork drive and adapter are horizontal.
The platform drive arm can be adjusted and the second hole in the adapter used to obtain more or less rocking angle or to achieve
a perfectly horizontal platform at mid-rock. If the length of the arm is adjusted, be sure to retighten the locking nuts on the arm. To
operate the rocking platform, turn the rotisserie power switch to the On position and adjust the speed using the rotisserie speed knob.
Instruction Manual
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