SynOptics SYNBIOSIS aCOLyte3 User Manual
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SynOptics SYNBIOSIS aCOLyte3 User Manual

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aCOLyte3
User Manual
All possible care has been taken in the preparation of this publication, but Synoptics Limited accepts no liability for any
inaccuracies that may be found.
Synoptics Limited reserves the right to make changes without notice both to this publication and to the product that it
describes.
No part of this publication may be reproduced, stored in a retrieval system or transmitted in any form or by any means
electronic, mechanical, photocopying, recording or otherwise without the prior permission of the copyright owner.
Version 13.p.1
Published by Synoptics Limited
© Synoptics Limited 2013

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Summary of Contents for SynOptics SYNBIOSIS aCOLyte3

  • Page 1 User Manual All possible care has been taken in the preparation of this publication, but Synoptics Limited accepts no liability for any inaccuracies that may be found. Synoptics Limited reserves the right to make changes without notice both to this publication and to the product that it describes.

  • Page 3: Table Of Contents

    Contents Getting started Introduction Structure of the Manual Using aCOLyte3 – overview Creating a batch Accepting a batch Taking measurements Starting aCOLyte3 Logging out and closing aCOLyte3 Capturing images The Image tab Loading plates into the instrument Capturing an image Setting the exposure Zooming the image Exporting the image to a file...
  • Page 4 Contents Dilution Pour Plate batches Spiral Plate batches Setting count restrictions Test Measure Plate The Results tab Accepting the batch design Managing batches Exporting batch details Deleting batches Using Measurement Mode to count colonies Opening and selecting batches Adjusting frame settings in Measurement mode Entering plate identifiers and dilutions Auto-incrementing plate identifiers Manually entered plate identifiers...
  • Page 5 Contents System settings Display properties Managing aCOLyte3 users Windows password security aCOLyte3 user groups Local and Domain/Security groups Database location Calibration Camera measurements Contents–iii aCOLyte3 User Manual...

  • Page 7: Getting Started

    Getting started Introduction aCOLyte3 is a combined hardware and software system for automatically counting colonies on plates. Once the system has been set up, all you have to do to count the colonies is to put each plate on the plate holder and press a button. The counting of each plate takes no more than a few seconds and the results are shown instantly in a table.

  • Page 8: Using Acolyte3 - Overview

    Getting started The rest of this Manual is structured as follows: The next section, Using aCOLyte3 – overview in this Getting Started chapter provides a brief overview of the way you use aCOLyte3 to count colonies. This involves two main phases: using the Batch Designer to set up the system for detecting and counting colonies on a series of plates;...

  • Page 9: Creating A Batch

    Starting aCOLyte3 Creating a batch In many cases, all you need to do to set up the colony detection procedure for a batch is specify whether the colonies are lighter or darker than the background, but if required, you can also fine tune the colony detection by adjusting the sensitivity, setting a minimum size for colonies and choosing whether to use the colony splitting algorithm.
  • Page 10 Getting started 4. Plug in the software dongle. 5. Press the black button on the front of the instrument to switch the light on – the lighting can be cycled between using the lower lights, the upper light or both the upper and lower lights by repeatedly pressing this button.

  • Page 11: Logging Out And Closing Acolyte3

    Logging out and closing aCOLyte3 Logging out and closing aCOLyte3 To log out from aCOLyte3 (this only applies if user authentication is enabled – see Managing aCOLyte3 users , page 95): Press the Log out button near the top right-hand corner of the aCOLyte3 application window: The Please log on to aCOLyte3 dialog box will be displayed.
  • Page 12 Getting started aCOLyte3 User Manual...

  • Page 13: Capturing Images

    Capturing images This chapter shows you how to capture and work with images. You will find out about: The Image tab , below Loading plates into the instrument , on the next page Capturing an image , page 9 Setting the exposure , page 9 Zooming the image , page 10 Exporting the image to a file , page 11 Importing an image from a file , page 11...

  • Page 14: Loading Plates Into The Instrument

    Capturing images You use the controls on the Image tab to capture and work with images: The View Batch Images button is enabled when you are working in Measurement mode (see Using aCOLyte3 – overview , page 2, for more information about the Batch Designer and Measurement mode).

  • Page 15: Capturing An Image

    Capturing an image 4. Re-attach the curtain around the sample stage. Capturing an image To capture an image in aCOLyte3: 1. Press the Image tab to display the image controls. 2. Press The image will be captured and displayed in the aCOLyte3 window. Note In fact, three images will be captured, with red, green and blue illumination respectively, and the results combined to form a color image –...

  • Page 16: Zooming The Image

    Capturing images 2. The current exposure time is shown between the arrow buttons. To change the exposure time, press: to decrease the exposure by a large step to decrease the exposure by a small step to increase the exposure by small step to increase the exposure by a large step.

  • Page 17: Exporting The Image To A File

    Exporting the image to a file Exporting the image to a file To export the current captured image in the aCOLyte3 window to a file: 1. Press the Image tab to display the image controls. 2. Press to display the Export Image dialog box – this is a standard Windows ‘Save As’ dialog box.

  • Page 18: Calibrating The Image

    Capturing images A warning dialog box will be displayed asking you to calibrate the image – see the next section, Calibrating the image , for details. Calibrating the image When you import an image from a file (see Importing an image from a file , on the previous page) or try to perform a measurement from an uncalibrated imported image, a warning dialog box will be displayed asking if you want to calibrate the image, for example: Note...
  • Page 19 Importing an image from a file 3. Move the pointer to the other end of the feature – as you do this, a line will be drawn back to the starting point, for example: 4. Click to mark the other end of the feature. The calibration edit box and units button will be displayed: 5.

  • Page 20: Viewing Batch Images

    Capturing images 6. Type the length of the known feature into the calibration edit box: 7. Press to set the calibration and return to the main aCOLyte3 window. Viewing batch images By default (see System settings , page 92), when you create a new batch, the image used to set it up (see Setting up batches , page 24) is saved with the batch, and then, after you have accepted the batch design, each time you take a measurement in Measurement mode, the captured image is also saved with the result in the batch.
  • Page 21 Viewing batch images 3. To export all the batch images: a. Press to display the Browse for Folder dialog box. b. Select the folder you want to export the images to – if required, press Make New Folder to add a new folder to the selected folder. c.
  • Page 22 Capturing images aCOLyte3 User Manual...

  • Page 23: Creating A New Batch

    Creating a new batch This chapter shows you how to create and set up a new batch for counting colonies on plates. It also describes a number of batch management procedures for use once you have created the batch. The process of creating a new batch is divided into two main stages, which are covered in the following main sections: Creating the batch –...

  • Page 24: Creating A Completely New Batch

    Creating a new batch You can create: A completely new batch from scratch – see Creating a completely new batch , below. A new batch based on an existing batch or an exported batch file – see Creating a new batch based on an existing batch or an exported batch file , page 21.
  • Page 25 Creating the batch to display the Plate Configuration dialog box: If you are going to use the batch for spiral plates, go to Step c; otherwise, go to Step b. b. For pour plates: i. Click on the Standard Plates tab if it is not already selected – see picture in Step a.
  • Page 26 Creating a new batch vi. To choose the units for the Sample volume, press the units button to display the units menu and select the required unit. vii. Press OK to confirm the settings, close the dialog box and return to the Launch Batch Designer dialog box –...

  • Page 27: Creating A New Batch Based On An Existing Batch Or An Exported Batch File

    Creating the batch The Batch Designer will be launched for the selected plate type so that you can complete the process of setting up the new batch – see Setting up batches , page 24, for details. Creating a new batch based on an existing batch or an exported batch file See Creating a completely new batch , page 18, for how to create a new batch from scratch.
  • Page 28 Creating a new batch to display the Base on Existing Batch dialog box: Note Any batches that are currently open will be marked with in the Available column. You will not be prevented from choosing to base the new batch on one of these, but you will be warned when you confirm the selection –...
  • Page 29 Creating the batch f. If you chose an existing batch that is currently in use, you will be warned: Press Yes to create the new batch anyway; press No to close the warning dialog box and return to Step c to choose another batch. The new batch will be created;...

  • Page 30: Setting Up Batches

    Creating a new batch Note You will not be able to edit the settings made in the Plate Configuration dialog box when the original batch was created – see Creating a completely new batch , page 18, for details of the settings made in the Plate Configuration dialog box.

  • Page 31: Giving The Batch A Name

    The Classification tab The Measure tab , page 47 The Configuration tab , page 35 The Results tab , page 54. Giving the batch a name After you have created a new batch (see Creating the batch , page 17), you must give it a name before you can accept the design (see Accepting the batch design , page 56).

  • Page 32: Frame

    Creating a new batch In order to use the Classification tab, you will need a sample image to work with: 1. Insert a typical plate from the batch into aCOLyte3 – see Loading plates into the instrument , page 8. 2.
  • Page 33 The Classification tab To change the type of frame used to define the region for counting colonies: 1. Press the frame button to display the frame menu. 2. Choose the required option – the contents of the menu depend on the type of plate and are described in the following sections: Pour Plate batches , below Spiral Plate batches , below.

  • Page 34: Adjusting The Position And Size Of Frames

    Creating a new batch Adjusting the position and size of frames The following sections describe how to adjust the position and size of frames: Moving frames , below Resizing frames , below Rotating frames , on the facing page. Note You should load a plate from the batch into the instrument and capture an image before adjusting the frame –...
  • Page 35 The Classification tab The frame boundary will turn orange – showing that you can adjust its size. For example: 2. Drag the frame boundary to resize/reshape the frame. For rectangular frames, drag the top or bottom side to adjust the height; drag the left or right side to adjust the width;...

  • Page 36: Total Plate Count

    Creating a new batch An orange line will appear joining the pointer to the center. For example: 2. Drag the line around the center to rotate the frame. Total Plate Count You can use the Total Plate Count button to set simple criteria for detecting and counting the total number of colonies on a plate.
  • Page 37 The Classification tab Note If there is no current image, an image will be automatically captured when you press the Total Plate Count button. If the image shown in the viewer is too light or too dark, press the Image tab and adjust the exposure as required (see Setting the exposure , page 9), then go back to Step 1.
  • Page 38 Creating a new batch If required, you can change the symbol and color used to mark the colonies – for details, see the next section, The Classification panel . 3. To adjust the settings used when counting colonies (if the button below the image is labeled All Colony Markers, All Colony Outlines or All Colony Regions the detected colonies will be shown on the image when you make the adjustments): a.

  • Page 39: The Classification Panel

    The Classification tab d. To set a lower limit to the size colonies must be in order to be counted (you can use this, for example, to prevent small particles of debris being counted as colonies): Drag the Reject Small Particles slider; click the arrow buttons at the ends of the slider to adjust the slider by a single step;...
  • Page 40 Creating a new batch To change the colony marker or color: 1. Move the pointer over the colony item to display the colony button: 2. Press the colony button to display the marker controls: 3. Either: Select the shape of colony marker you want to use from the marker controls. a.

  • Page 41: The Configuration Tab

    The Configuration tab The Configuration tab Note The Configuration tab does not appear in Measurement mode. Note The Use Barcodes check box is disabled if there is no barcode reader attached to the PC. To display the configuration controls: Press the Configuration tab: The Configuration tab in the Batch Designer allows you to specify how plate identifiers and, where relevant, dilutions should be assigned to plates in measurement mode.
  • Page 42 Creating a new batch If the batch you are creating is based on another batch, the Configuration tab shows the settings selected in the parent batch: The green check marks show the settings selected in the parent batch – if the parent batch uses manually entered parent plate identifiers, the Parent is using auto-increment will have a red cross next to it, but its check box will be enabled.

  • Page 43: Manually Entered Plate Identifiers

    The Configuration tab A plate list of plate identifiers and, optionally, dilutions Each identifier (and dilution) in the list is assigned to each of the plates in turn as they are measured. You can enter a plate list by hand or import it from a file. See Using an external source for plate identifiers , page 42, for details.
  • Page 44 Creating a new batch 3. If the icon in the Plate List box is go straight to Step 4. Otherwise, an external source of plate identifiers has been selected for the batch and the Auto-Increment control will be disabled. To enable the Auto-Increment control and deselect the external source: a.

  • Page 45: Selecting Auto-Incrementing Plate Identifiers

    The Configuration tab Selecting auto-incrementing plate identifiers Note This option is selected by default when you create a new batch from scratch. To use auto-incrementing plate identifiers: 1. Press to display the Configuration tab: 2. If the new batch is based on an existing batch, there will be a set of Parent Batch controls below the Plate List controls.
  • Page 46 Creating a new batch To enable the Auto-Increment control and deselect the external source: a. Press to display the Use External Source for Plate ID dialog box: b. Press 4. If Auto-increment is not selected in the Plate ID box, click the check box to select it. 5.

  • Page 47: Using A Barcode Reader For Plate Identifiers

    The Configuration tab Using a barcode reader for plate identifiers Note Depending on how the barcode reader is programmed, the barcode reader may or may not be able to read dilution information. When you are working in Measurement mode, you can use a barcode reader to read the identifier/dilution from the plate in combination with an external source of plate identifiers (see Using an external source for plate identifiers , on the next page) or to input manual identifiers (see Manually entered plate identifiers , page 64).

  • Page 48: Using An External Source For Plate Identifiers

    Creating a new batch Using an external source for plate identifiers You can use the following external sources for plate identifiers: a CSV file a list created in aCOLyte3. To specify an external source for plate identifiers: 1. Press to display the Configuration tab: 2.

  • Page 49: Using A Csv List Of Identifiers

    The Configuration tab to display the Use External Source for Plate ID dialog box: 3. Press the button for the required option. The following sections describe each of the options in turn: Using a CSV list of identifiers , below Creating a plate list , page 45.
  • Page 50 Creating a new batch in the Use External Source for Plate ID dialog box (see Using an external source for plate identifiers , page 42) to display controls for opening the CSV file: 2. Press to open a standard Windows Open dialog box. 3.

  • Page 51: Creating A Plate List

    The Configuration tab Check the preview box to make sure the contents are correct (see the subsection following these instructions for the format to use for the CSV file). 6. If the contents are correct, press OK to close the Use External Source for Plate ID dialog box and load the plate list into the batch.
  • Page 52 Creating a new batch in the Use External Source for Plate ID dialog box (see Using an external source for plate identifiers , page 42) to display controls for creating a plate list: 2. Type the identifier for the first plate into the edit box at the top of the dialog box. 3.

  • Page 53: The Measure Tab

    The Measure tab Note When you are working in Measurement mode, you can use a barcode reader in conjunction with a custom plate list to match each plate you are measuring to its identifier/dilution in the plate list – see Using a barcode reader for plate identifiers , page 41. The Measure tab Notes The picture shows the Measure tab for a Pour Plate batch: for Spiral batches, the dilution...

  • Page 54: Plate Id

    Creating a new batch carry out a test count of the current plate (see Test Measure Plate , page 53) so that you can check the batch settings. Plate ID The Plate ID control on the Measure tab in the Batch Designer allows you to specify a template for auto-incrementing plate identifiers –...

  • Page 55: Pour Plate Batches

    The Measure tab Pour Plate batches See Spiral Plate batches , on the next page, for how to set the dilutions for Spiral Plate batches. Note To set the dilution for a Pour Plate batch: 1. Press (the button shows the current dilution) to open the dilution editor: 2.

  • Page 56: Spiral Plate Batches

    Creating a new batch Spiral Plate batches See Pour Plate batches , on the previous page, for how to set the dilutions for Pour Plate Note batches. To set the dilutions for a Spiral Plate batch: 1. Press the dilutions button to display the dilutions menu (the button shows the current dilution): Note The menu contains the dilutions in the current dilution list for the batch.
  • Page 57 The Measure tab The dilution list in the file will be added to the Dilution List Editor, replacing any existing entries. 4. To add a new dilution to the list: a. Click on the dilution at the point you want to add the new dilution. b.

  • Page 58: Setting Count Restrictions

    Creating a new batch Note When you carry out a test measurement for a Spiral Plate batch in the Batch Designer, the dilution selected in the dilutions menu affects the results, which show a count/ml value – see Test Measure Plate , on the facing page. Dilutions file format Each line of the file should consist of a string representing the dilution (if the string is not recognized or no dilution is given, ‘No Dilution’...

  • Page 59: Test Measure Plate

    The Measure tab 5. Check Exclude from mean if you want any results violating the count restrictions to be ignored when calculating the mean results; leave it unchecked to include all results in mean calculations. When count restrictions have been set and you carry out a test measurement in the Batch Designer (see Test Measure Plate , below) or perform a measurement in Measurement mode, the Measurement test results or Last measurement results box, respectively, will show whether the limit has been breached, for example:...

  • Page 60: The Results Tab

    Creating a new batch All Colony Regions: the area of each detected colony is marked in the color of the symbol representing the type of colony. If required, you can zoom the image to check the colony detection – see Zooming the image , page 10.
  • Page 61 The Results tab To display the results controls: Press the Results tab: You can set a default selection of results in the Batch Designer, but you can also make a different selection at any time in Measurement mode as required. Results with a selected check box will be included in the Results table;...

  • Page 62: Accepting The Batch Design

    Creating a new batch Accepting the batch design Once you have accepted a batch for use in Measurement mode (see Accepting the batch design , above), you will not be able to change whether aCOLyte3 detects light or dark colonies. However, you will be able to carry out a test measurement and change the settings for the Reject Small Particles and Sensitivity sliders and the colony splitter before recording the result (see Test measurement and adjusting settings , page 72).

  • Page 63: Managing Batches

    Managing batches Managing batches Exporting batch details To export the details of a batch to an XML file: 1. Press the aCOLyte3 button at the top left-hand corner of the window to display the aCOLyte3 menu: 2. Press: to display the Export Data dialog box – this is a standard Windows Save As dialog box. 3.
  • Page 64 Creating a new batch 3. Press: to display the Batch Management submenu. 4. Press: to display the Delete Batch dialog box listing The 10 most recent batches: 5. If the required batch is not in the list of most recent batches: a.
  • Page 65 Managing batches You will be asked to confirm that you want to delete the batch: 8. Press Yes to delete the batch. You will be asked if you want to delete the image files: 9. Press Yes to delete the image files saved with the batch. Note Although the batch can no longer be viewed in aCOLyte3, it is not actually removed from the aCOLyte3 database and can be viewed there if required for audit purposes.
  • Page 66 Creating a new batch aCOLyte3 User Manual...

  • Page 67: Using Measurement Mode To Count Colonies

    Using Measurement Mode to count colonies Once you have created and accepted a new batch (see Creating a new batch , page 17, and Accepting the batch design , page 56), the general procedure for counting colonies is: 1. Open or select the batch – see the next section, Opening and selecting batches . 2.
  • Page 68 Using Measurement Mode to count colonies to display the Open Batch dialog box listing The 10 most recent batches: Open batches are marked with a in the Available column. 2. If the required batch is not in the list of most recent batches: a.

  • Page 69: Adjusting Frame Settings In Measurement Mode

    Adjusting frame settings in Measurement mode to display the Review Open Batches dialog box: The currently selected batch is listed with a check box in the Current Batch box, and there are buttons and check boxes for the Other Open Batches in the box below it. 2.

  • Page 70: Auto-Incrementing Plate Identifiers

    Using Measurement Mode to count colonies Note If several plates are given the same identifier (for example, with different dilutions of the same sample), they will be grouped together under the same item in the Results table – see Working with results , page 77, for details.

  • Page 71: Changing The Dilution Set For A Plate

    Entering plate identifiers and dilutions Changing the dilution set for a plate If required, you can change the dilution set for the current plate. The procedure required depends on the type of batch. Pour Plate batches To change the dilution for the current plate: 1.

  • Page 72: External Source - Csv File Or Custom Plate List

    Using Measurement Mode to count colonies 1. Press the dilution button, for example: 2. Select the required dilution from the Dilution List. To edit the dilution list and choose a dilution from the new list: 1. Press the dilution button, for example: 2.
  • Page 73 Entering plate identifiers and dilutions When you first open the batch, the Next Plate ID box shows the first plate identifier on the list and, where relevant, the dilution control shows the dilution, for example: Note The Next Plate ID text box is disabled for a batch using an external source for its plate identifiers –...

  • Page 74: Using A Barcode Reader For Identifiers And Dilutions

    Using Measurement Mode to count colonies 2. Click in the row containing the required plate identifier/dilution to select it. 3. Press OK to close the dialog box – the selected identifier will appear in the Next Plate Id box and the dilution will appear on the dilution button. Using a barcode reader for identifiers and dilutions You can use a barcode reader to enter a manual plate identifier (and dilution if the barcode reader is programmed to read dilutions) or pick a plate identifier and dilution from the plate...

  • Page 75: Counting The Colonies On A Plate Automatically

    Counting colonies Note The picture shows the Measure tab in Measurement Mode for a Pour Plate batch – the Manual Count, Exclude Region and Remove Last Exclusion buttons do not appear for Spiral Plate batches. The Adjust button is only enabled after you have pressed Test Measure Plate and the Remove Last Exclusion is only enabled if you have placed an exclusion region on the image.
  • Page 76 Using Measurement Mode to count colonies ignored when aCOLyte3 counts the colonies – the area of the exclude regions will be deducted from the area of the frame to ensure that results such as count/ml are correct. Note An alternative for Pour Plate batches might be to choose a Partial Plate frame and/or to rotate the frame so that the problem area is not counted.
  • Page 77 Counting colonies 4. Move the pointer to the position you want to place the next vertex of the exclude region – a line will be dragged out from the first vertex: 5. Click to add the new vertex for the exclude region. 6.

  • Page 78: Test Measurement And Adjusting Settings

    Using Measurement Mode to count colonies 8. Double-click to add the vertex and complete the region. To remove the last exclude region you added to the image: Press You can repeat these procedures to add and remove as many exclude regions as required. Test measurement and adjusting settings If required, you can carry out a test measurement to check the colony detection for an individual plate without recording the result, and, if required, you will be able to modify the...
  • Page 79 Counting colonies The result of the test measurement will appear in the Last Measurement Results panel and the Adjust button will be enabled: The result of the test measurement will not be added to the Results table (see The Results table , page 77). If the button below the image is labeled All Colony Markers, the detected colonies will also be marked on the image using the colors and markers selected for each colony type (if the button is labeled No Colony Markers, press the button and select All Colony...

  • Page 80: Manual Colony Counting

    Using Measurement Mode to count colonies 4. To carry out the measurement and record the results in the Results table, press Note If you make any changes to the settings using the procedures described in this section, the new settings will be applied to the batch until you close the batch. If you wish to revert to the settings made when the batch was created, either make a note of the values and re-enter them using the above procedure, or close the batch (see Closing batches , on the facing page) and reopen it again (see Opening and selecting batches , page 61).

  • Page 81: Entering A Zero Count

    Closing batches Entering a zero count To enter a zero result for a plate: Press A new result will be added to the Results table with a 0 count. Note The image will not be saved with the result, even if Save images to file is selected in the Settings dialog box –...
  • Page 82 Using Measurement Mode to count colonies to display the Review Open Batches dialog box: The currently selected batch is listed with a check box in the Current Batch box, and there are buttons and check boxes for the Other Open Batches in the box below it. 2.

  • Page 83: Working With Results

    Working with results This chapter shows you how to view and process the results produced by aCOLyte3, and then compile a report based on them (see Using Measurement Mode to count colonies , page 61, for how to carry out the measurements). This chapter has three main subsections: The Results table , below Editing plate measurements , on the next page Compiling reports , page 88.

  • Page 84: Editing Plate Measurements

    Working with results If there is more than one result for a Plate Name, you can choose how much information to show by opening or closing the Plate Name, the Mean result or the Plates details with in it, for example: To open of close a plate name or some of the details within it: Press the or button next to the item you want to open or close.
  • Page 85 Editing plate measurements To edit a measurement: 1. Select the measurement you want to edit by clicking in the plate's result in the Results table – see The Results table , page 77. 2. Press the Edit Result tab to display the Editing Plate panel at the bottom of the aCOLyte3 window: and the Edit Result controls in the Edit Result tab.
  • Page 86 Working with results The controls included on the Edit Result tab depend on the type of batch. For example, for a Pour Plate batch, you get the following controls: Notes The Exclude Region, Clear Exclusion and Adjust Frame controls are hidden for Spiral Plate batches.

  • Page 87: Editing The Plate Identifier, Comments And Dilution

    Editing plate measurements Adjusting the frame for a result , page 84 Rejecting small particles in a result , page 84 Changing the Sensitivity setting for a result , page 85 Splitting colonies in a result , page 85 Adding exclude regions to a result , page 86 Zeroing the count , page 87.

  • Page 88: Saving Changes To Results

    Working with results The identifier, comment and/or dilution will be updated for the result and if you have changed the dilution, values depending on the dilution will also be updated. An E flag will appear in the Flags column showing the result has been edited. Saving changes to results To save any changes you have made to a result on the Edit Result tab and/or the Editing Plate panel (see Editing plate measurements , page 78):...

  • Page 89: Viewing The Audit History Of Changes

    Editing plate measurements Note Although the result is deleted from the Results table and can no longer be viewed in aCOLyte3, it is not removed from the aCOLyte3 database and can be viewed there if required for audit purposes. Viewing the audit history of changes When you make any changes to a result, aCOLyte3 makes an entry in the audit history for that plate.

  • Page 90: Adjusting The Frame For A Result

    Working with results If you want to remove colonies, press 4. If you have chosen to add colonies, click on the image at the point you want to add the colony – you cannot add colonies outside the frame. If you have chosen to remove colonies, click on the colony markers you want to remove.

  • Page 91: Changing The Sensitivity Setting For A Result

    Editing plate measurements To change the Reject Small Particles filter setting for an individual plate result: 1. Select the Edit Result tab for the plate result – see Editing plate measurements , page 78. 2. Drag the slider; click the arrow buttons at the ends of the slider to adjust the slider by a single step;...

  • Page 92: Adding Exclude Regions To A Result

    Working with results This section shows you how to change the splitter setting for an individual plate after it has been measured. To change the colony splitter setting(s) for an individual plate result: 1. Select the Edit Result tab for the plate result – see Editing plate measurements , page 78. 2.

  • Page 93: Zeroing The Count

    Editing plate measurements 3. You define the exclude region in the same way as when you add exclude regions before making a measurement – see Exclude regions , page 69. The plate will be remeasured with the new exclude region in place. 4.

  • Page 94: Compiling Reports

    Working with results Compiling reports To compile a results report: 1. Press in the Results tab to display the aCOLyte3 Reports dialog box: 2. Click on the template for: CSV Report to save a report on the current batch including system settings to a CSV file the current batch (labeled by the batch type) to create a detailed report for the current batch...
  • Page 95 Compiling reports To include all the data for each result, including columns not selected for the Results table (see The Results tab , page 54): Click Restrict report by batch headings so that it is not checked. Note The Restrict report by batch headings setting has no effect on summary or audit trail reports. 4.
  • Page 96 Working with results aCOLyte3 User Manual...

  • Page 97: Configuring Acolyte3

    Configuring aCOLyte3 This chapter shows aCOLyte3 administrators how to configure aCOLyte3 in a variety of ways. You will find out about: Displaying information about the program version you are using – see About aCOLyte3 , below Using the Settings dialog box to configure the program – see System settings , on the next page Changing the color of the frame –...

  • Page 98: System Settings

    Configuring aCOLyte3 to display the About aCOLyte3 dialog box: 3. Press Save Details to display a standard Windows Save As dialog box if you want to save the program version details in a text file. 4. Press Close to close the dialog box. System settings To view or change the system settings: 1.
  • Page 99 System settings 2. Choose System Settings from the Settings submenu to display the Settings dialog box: 3. Enter the name of your organization under Company Name. The Company Name is included in reports – see Compiling reports , page 88. 4.

  • Page 100: Display Properties

    Configuring aCOLyte3 6. Set the Export Path for the folder used to save exported batch details (see Exporting batch details , page 57) by typing the path directly into the box, or press to display a standard Windows Browse For Folder dialog box so that you can select the required folder.

  • Page 101: Managing Acolyte3 Users

    Managing aCOLyte3 users 2. Choose Frame Color or Frame Color (border), from the Settings®Display Properties submenu to display the Color palette, for example: 3. Either click on one of the Standard Colors in the palette to select it or press More Colors to display a color picker allowing you to choose from a wider range of Standard colors or to create your own Custom color.

  • Page 102: Windows Password Security

    Configuring aCOLyte3 to log on to that PC) audit trails will record which aCOLyte3 user made any changes. If you choose Local User Groups, you will need to: add Windows user names and passwords for all the users who are going to use the aCOLyte3 installation –...

  • Page 103: Acolyte3 User Groups

    Managing aCOLyte3 users aCOLyte3 user groups If the Authentication Method is set to Local User Groups or Network User Groups in the Settings dialog box (see System settings , page 92), in order to gain access to aCOLyte3, a user must: a.
  • Page 104 Configuring aCOLyte3 3. Open Computer Management, expand Local Users and Groups and select Groups: 4. To add users to the Acolyte Users group: a. Right-click on the required group in the right-hand pane to display a context menu. b. Choose Add to Group to display the Group Properties dialog box: The example shows the default aCOLyte Users group, which contains the A3Admin user by default.
  • Page 105 Managing aCOLyte3 users c. Press Add to display the Select Users dialog box: Either: i. Type the user name into the box; if required, you can enter multiple user names by typing them on the same line but separated by semi-colons. ii.

  • Page 106: Database Location

    Configuring aCOLyte3 d. Press OK to close the Select Users dialog box and add the users to the group in the Properties dialog box. Note If required, you can also use the Properties dialog box to remove users from the group by selecting them and pressing Remove.

  • Page 107: Calibration

    Calibration A dialog box will be displayed warning you that aCOLyte3 will restart: 5. Press OK. aCOLyte3 will close down and restart automatically connected to the new database location. Calibration aCOLyte3 is fully calibrated in the factory and in normal circumstances will require no further calibration after delivery.
  • Page 108 Configuring aCOLyte3 aCOLyte3 User Manual...
  • Page 109 Index Configuration tab 35-47 Count restrictions 52-53 About aCOLyte3 91-92 Counting colonies 61, 68-75 Accept New Batch 56 automatically 69 aCOLyte3 outline procedure 61 icon 4 Creating a new batch 17-24 logging out/closing 5 from existing Batch 21-24 overview 2-3 from exported batch 21-24 starting 3-4 aCOLyte3 user groups 97-100...
  • Page 110 Index Results 54-55, 77-89 adjusting the frame 84 Image files 93 changing order 55 Image tab 7-8 deleting 82-83 Images editing 78-87 calibrating 12-14 including/excluding 55 capturing 7, 9 resetting changes 82 exporting 11 saving changes 82 importing 11-12 undoing changes 82 zooming 10 zeroing 87 Importing images 11-12...

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