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BlueLine
Instruments for Electrophoresis
I
M
NS T RUCT I O N
ANU AL
___________________________________________________________________
HPE™ BlueHorizon
Flatbed Electrophoresis System
(Cat. No.: HPE-BH)
SERVA Electrophoresis GmbH
Carl-Benz-Str. 7
D-69115 Heidelberg
Phone +49-6221-138400, Fax +49-6221-1384010
e-mail: info@serva.de
http://www.serva.de
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Table of Contents
Related Manuals for serva Blue Series
Summary of Contents for serva Blue Series
- Page 1 BlueLine Instruments for Electrophoresis NS T RUCT I O N ANU AL ___________________________________________________________________ HPE™ BlueHorizon Flatbed Electrophoresis System (Cat. No.: HPE-BH) SERVA Electrophoresis GmbH Carl-Benz-Str. 7 D-69115 Heidelberg Phone +49-6221-138400, Fax +49-6221-1384010 e-mail: info@serva.de http://www.serva.de...
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Page 3: Table Of Contents
Content Safety ........................3 Introduction ......................4 Installation ......................5 Operation ......................6 Adjust the electrodes ..................6 Apply a gel ..................... 6 Prepare Electrode Wicks ................7 4.3.1 SDS Gel Kit, CleanGel, HPE Gel ............7 4.3.2 PreCotes, PreNets, CleanGel IEF ............8 4.3.3 FocusGel.................... -
Page 4: Safety
1 Safety The SERVA HPE BlueHorizon comply with the standards and directives mentioned in the applicable CE declaration. Please take note of the following safety measures: Warning: Operation of this instrument requires high voltage. Disconnect the high voltage external power supply before opening any drawer. -
Page 5: Introduction
Instead, fabric wicks are soaked with concentrated electrophoresis buffers. The integrated drawer holds the cooling plate that is connected to the SERVA HPE™ Cooling Unit (cat. no. HPE-CU1). The cooling plate is made from a special ceramic material (maximum gel size 260 x 205 mm) for efficient heat conductance down to 4 °C resulting in rapid and straight migration and therefore highly focused spots and... -
Page 6: Installation
Place the base unit on the bench. Unevenness of the lab bench can be corrected by using a spirit level and adjusting the levelling feet. Connecting the SERVA HPE™ Cooling Unit (Chiller) Important note: Warm air should not be exhausted towards the BlueHorizon! Connect the BlueHorizon to the chiller using the provided tubing and fix them with the hose clamps. -
Page 7: Operation
In this section, general instructions for loading and running gels on the HPE BlueHorizon are described. Running conditions for specific gel types, videos, and other useful information are provided on the enclosed DVD and on www.serva.de. Important Information: Always wear powder free disposable gloves when handling gels or stripes. -
Page 8: Prepare Electrode Wicks
4.3 Prepare Electrode Wicks 4.3.1 SDS Gel Kit, CleanGel, HPE Gel All buffers needed are provided in the SERVA Gel and Buffer Kits. We do not recommend different or self-made components. Electrode wicks, soaked in an appropriate buffer, provide a convenient alternative to buffer tanks. -
Page 9: Precotes, Prenets, Cleangel Ief
4.4.3 HPE Gel 1) Equilibrate the IPG strip SERVA IPG strip equilibrator (Cat. no. HPE-A04) provides a convenient way to equilibrate IPG strips. After equilibration, the strip can be easily transferred from the slots holding the first equilibration solution (e.g. DTT) to the slots holding the... - Page 10 Prepare the two equilibration solutions from the SERVA IPG Strip equilibration buffer: DTT solution: Weigh urea and Dithiothreitol (DTT), add the equilibration buffer and dissolve completely. IAA solution: Weigh urea and Iodoacetamide (IAA), add the equilibration buffer and dissolve completely.
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Page 11: Precotes, Prenets
4.4.4 PreCotes, PreNets 1) Unpack the gels and remove the protective cover-film from the gel surface. Keep the cover film as it can serve as a protective sheet later. 2) Adjust sample concentration to about 1 - 10 mg protein/ml and desalt by dialysis; by dilution with dest. -
Page 12: Start The Run
4.5 Start the run 1) Close the lid while lowering the electrodes on the wicks. Plug in the leads. 2) Switch the thermostatic circulator on, set to HPE Gels: 15 °C SDS Gels: 15 °C Clean Gels: dependent on the application (see gel manual) PreCotes, PreNets: 10°C CleanGel IEF: 10°C Focus Gel: dependent on the application (see gel manual) -
Page 13: After The Run
After electrophoresis, clean the device as described and follow with staining and detection. We recommend: Staining Application Cat. No. SERVA HPE™ Silver Staining Kit (MS compatible) 2D 43395 SERVA CSF Silver Staining Kit CSF IEF 43398 SERVA Silver Staining Kit Native PAGE... -
Page 14: Maintenance
5 Maintenance 5.1 Cleaning Regularly clean the housing of the SERVA HPE BlueHorizon. Precautions for avoiding electric shock Electronic devices can cause electric shocks in case of an operating error. Never try to repair electric parts. Never open the housing. -
Page 15: Technical Data
5. Wipe off all outer parts of the instrument with the disinfectant and a lint-free cloth. Decontamination before shipment If you need to send the instrument back to us, decontaminate all parts. Document this in our Decontamination Certificate (Download on www.serva.de) and include it within the shipment. 6 Technical data SERVA BlueHorizon Max. -
Page 16: Trouble Shooting
8 Trouble Shooting Symptom Cause Remedy 7.1 Effects during electrophoresis No water flow after Straighten tubing. Check tubing: Wrongly connected chiller is switched Chiller-“Out“ connected to chamber- tubing or kinked tube. “In“. Air bubbles between film- Insufficient volume of Lift up gel on one side and apply a backing and cooling cool contact fluid higher volume... - Page 17 The gel gets hot during electrophoresis See above because of insufficient heat dissipation. BlueHorizon is subject to hot exhaust from Front is curved Relocate chiller or other apparatus. chiller or other instead of straight. apparatus. The gel gets hot If you run less than four gels at a time, during electrophoresis reduce the mA and W settings in the because too much...
- Page 18 Strong 2D Gels: Run stops, electroendosmosis front does not effect at the IPG Remove IPG strip(s) from gel after the continue to migrate, strip(s), because it first 70 minutes and then continue the sparking at the IPG has not been removed run.
- Page 19 SERVAPurple Wrong protocol (for staining: non-backed gels) has Follow the protocol of this HPE Unsufficient been applied, or the Horizon manual which has been sensitivity of solution volumes were optimized for the HPE Flatbed gels. staining too small...
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Page 20: Addendum: Example Applications
SDS Gel Kit 10% 25S; Cat. No. 43359 Marker, muscle, E. coli extracts ® Coomassie staining SERVA Blue R Staining Kit; Cat No. 42531 1D native PAGE 52 slots CleanGel 10% 52S; Cat. No. 43340 Native Buffer; Cat. No. 43352 Tomato seeds ®... -
Page 21: Pack List And Order Information
43371 Optional: BluePower™ Supply Control Kit (Cat. no. BP-PCSV01) The BluePower™ Supply Control Kit provides all needed to linkany SERVA BlueLine Power Supply via an USB - serial converter to a personal computer with documentation software. It has basically two functions: 1. - Page 22 For electrophoretic transfer of proteins on a blotting membrane the film-backing must be removed from the gel. The easiest and safest way to remove the film-backing without damaging the gel layer is cutting it with a thin wire using the SERVA Gel Remover.
- Page 23 Dr. Barbara Müller Geschäftsführerin Chief executive officer Présidente-directrice générale __________________________________________________________________________________________ SERVA Electrophoresis GmbH – D-69115 Heidelberg – Carl-Benz-Str. 7 – Tel: +49 6221 13840-0 – Fax: -10 Geschäftsführer: Dr. Barbara Müller, Dr. Manfred Kurfürst – HRB 336136 AG Mannheim – E-Mail: Info@serva.de...
- Page 26 To place orders Carl-Benz-Str. 7 Phone: 06221 13840-0 Please contact your local D-69115 Heidelberg Fax: 06221 13840-10 Distributor Germany (please visit www.serva.de) SERVA Electrophoresis Customer Care Customer Care GmbH Phone: 06221 13840-46 Phone: +49 6221 13840-47 Pinnauallee 4 Fax: 06221 13840-10...
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