Quick Guide; Cell Count Procedure - DeNovix CellDrop FL User Manual

3. Quick Guide

Cell Count Procedure

1. Launch a Count app.
2. Lower arm to start live preview. Check to ensure that the field of view is clear.
3. Select a protocol.
Optional: Enter sample name and additional sample info.
Optional: Edit the Protocol Count Settings for the sample being measured. Check that the
protocol includes the correct Dilution Factor.
4. Mix the sample well. Load the sample volume indicated on the Count button. Slide the pipette
tip along the alignment groove until it rests against the top surface. Dispense the sample into
the counting chamber formed between the upper and lower sample surfaces.
5. Allow the cells to settle.
6. Touch the focus icon and then adjust while viewing the Brightfield channel. Live cells should
have a bright center and a dark outline. The image below on the left is correctly focused. The
image on the right is out of focus.
7. Adjust Trypan Exposure: Touch the exposure icon and select Normal or Low exposure. Live
cells should have a white center with a dark ring like the image below on the left. The middle
image is overexposed and the right image is underexposed.
8. Fluorescence Exposure: Select the green or red fluorescence channel. Touch the exposure icon
and then adjust the setting. The fluorescence signal should not extend outside of the
boundaries of the cell when viewed in brightfield. The image on the left is correct. The middle
image is overexposed and the right image is underexposed. Adjust the exposure for each
channel independently.
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