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Table of Contents
Related Manuals for DeNovix CellDrop FL
Summary of Contents for DeNovix CellDrop FL
- Page 1 December 2019...
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Page 2: Table Of Contents
1. Introduction 3 Product Technology 3 Key Features 3 Optics 3 2. Set-up and Safety 4 3. Quick Guide 6 Cell Count Procedure 6 Best Practices 7 4. General Operation and Software Information 8 Top Action Bar 8 Software Icons and Buttons 9 Chamber Height Options 9 Data Storage 9 5. Count Apps 10... -
Page 3: Introduction
This innovative, patented technology (US 10,302,568) along with user-defined protocols allows users the flexibility required for today’s cell culture workflows. This User Guide is designed for both CellDrop BF (Brightfield only) and CellDrop FL (Dual Fluorescence plus Brightfield) owners. Several applications and software features are only applicable for instruments with Fluorescence mode (CellDrop FL) and do not apply to the CellDrop BF. -
Page 4: Set-Up And Safety
2. Set-up and Safety Remove all packing materials including materials protecting the arm, sample surfaces and the protective cover on the screen. Place the instrument on a flat bench and avoid vibrations to ensure optimal performance. Plug the unit into a 100-240 VAC, 50-60/Hz receptacle using the grounded power supply (GlobTek, Inc. - Page 5 Cautions DO NOT REMOVE COVER: No operator serviceable components inside. Refer servicing to qualified personnel. CellDrop Cell Counters are designed for indoor use under the following conditions: Temperature: 15˚ to 35˚ C Humidity: 35 to 65 % Use only the power supply provided with the instrument. Use of the instrument in any manner not specified by the manufacturer may impair the protection provided by the supplied power cord and power supply.
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Page 6: Quick Guide
3. Quick Guide Cell Count Procedure Launch a Count app. Lower arm to start live preview. Check to ensure that the field of view is clear. Select a protocol. Optional: Enter sample name and additional sample info. Optional: Edit the Protocol Count Settings for the sample being measured. Check that the protocol includes the correct Dilution Factor. -
Page 7: Best Practices
• Avoid introducing air bubbles into the sample solution when mixing or dispensing samples. • Avoid drawing sample back into pipette tip after dispensing. • Refer to DeNovix Assay protocols or other manufacturer guidelines for assay specific instructions. -
Page 8: General Operation And Software Information
Print Options chapter for more information. o Count Code Log (PAYG models only): Displays a log of count code use. o Tech Support: Sends an email with diagnostic information to DeNovix. Email must be preconfigured from the Settings app for this function to work correctly. -
Page 9: Software Icons And Buttons
Software Icons and Buttons CellDrop applications include a set of standard icons and buttons used throughout the software. The following table defines each button. Home Select All Overflow Increase / Edit Menu Decrease Count Copy Export Exposure Delete Clear Selected Expand / Focus Information... -
Page 10: Count Apps
Optimize Settings button.. Channels Count apps use an application specific set of optical channels. The CellDrop FL model includes Brightfield, Green Fluorescence and Red Fluorescence channels. The CellDrop BF model includes Brightfield only. -
Page 11: Ao/Pi
The % cell viability is not provided when using this app. The PI app uses brightfield images to capture live cell counts and red fluorescence images for counting dead cells. Propidium iodide (PI) is a red-fluorescent nuclear and chromosome stain that only enters dead cells. Upon entry, the PI stain binds to DNA by intercalating between the bases with little or no sequence preference. -
Page 12: Custom Methods
Note: Yeast cells are smaller than typical mammalian cells and may take a little longer to settle into position. % Viability =(FDA count / (FDA count+ PI count)) *100 Custom Methods The Custom Methods app allows users to count cells using brightfield, green fluorescence or red fluorescence techniques. -
Page 13: Count Tab View
Image Controls • Channel Selector: For the CellDrop FL model, use the Channel Selector to switch between brightfield and fluorescence preview channels. Within fluorescence apps, the icon indicates the active channel. Tap the icon to select a channel. CellDrop BF units and brightfield applications (Brightfield, Trypan Blue) only include the brightfield channel. -
Page 14: Dilution Calculator
• Exposure : Touch the exposure icon to display the exposure control in the Trypan app or for fluorescence channels. The control is not required for brightfield counts which use a preset value. o Trypan Blue app: Choose the correct exposure based on the concentration of trypan dye used. -
Page 15: Count Button
• When applied, the inputs are used to generate the dilution guidance presented under the Single Sample view as well as the Results tab Results List view when the Show Dilution Guidance box is checked. Count Button • The Count button initiates a measurement. •... -
Page 16: Count Settings
7. Count Settings Each Count app includes an applicable group of count settings saved within protocols. Count settings may be adjusted prior to a count. A subset of the parameters can be also adjusted after a count to optimize a result. •... - Page 17 setting when counting larger cells if they are being counted as multiple smaller cells. Decrease if small cells are not being counted. o Maximum Diameter - Objects above the maximum diameter will not be counted. Adjust the maximum diameter to provide a realistic size band to analyze during counts. If clusters of cells are being counted as a large individual cell, reducing the maximum diameter to reflect the true cell size will improve the results.
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Page 18: Results Tab View
8. Results Tab View Optimize Settings • Once a sample has been counted, a subset of the protocol parameters can be further adjusted to optimize the result. Press the Optimize Settings button, adjust settings and then press Count with Current Settings to reanalyze the existing image(s) using the new settings. Users have the option to save the adjusted settings to a protocol if desired. -
Page 19: Cell Size Gating
Cell Size Gating • Use the on-screen sliders to adjust the size range of interest for the current sample. Cell outlines and count results are updated in real time. Gate to exclude debris or alternative cell populations from analysis. Press the Histogram button to graphically view cell size distribution and apply gating. -
Page 20: Results View
• Edit : Highlight a single sample and then tap the edit icon to add or modify sample information. Changes will overwrite the information stored in the database and will be applied in all lists referencing the sample. • Delete: Tapping this icon will delete the highlighted samples from all results lists. Results View •... - Page 21 o A color legend is included for the various histograms. Select up to 5 samples at a time to chart. o Graph Type Menu: Use the drop down to select additional graph types. Graph Types • The graph types available are app dependent. Graphs may be expanded to full screen for easier viewing using the expand/collapse button.
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Page 22: Export And Print Options
Data Export for results and protocol settings, images, and histograms Note: To open an exported .csv file on a computer, the DeNovix instrument and the computer must both be set to use either period or comma number formats. The CellDrop number format is determined by the language selected using the Settings app. -
Page 23: Utility Apps
Utility Apps User Accounts The User Accounts app allows users to create and manage individual accounts on CellDrop. Users can create user-specific protocols and default settings. Accounts are also used as a means of segregating data when performing searches in the Data app. For Pay-As-You-Go models, users may also define how count codes are shared amongst user groups. -
Page 24: Data
Contact the holder of an administrator account if a standard user account password is forgotten. • Contact DeNovix customer support or your local distributor if the Primary Administrator password is forgotten. Please have the serial number of the instrument available when requesting help for the Primary Administrator password reset. -
Page 25: Count Codes
• Enter New Code • Review Code History Please include the serial number of your instrument when contacting DeNovix by email to purchase additional count codes. Outside of the US, please contact your local distributor for assistance. Calculator This app is not yet implemented and will be included in a future software release. - Page 26 The Settings app may be found on the second Home screen page. Only administrator accounts may access and change settings. The app provides access to the following functions: System Info • View software and firmware versions, IP addresses, current language and hostname. Date/Time •...
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Page 27: Updater
Email Password Examplegmail123 Test Email To info@denovix.com Refer to your local IT support group if additional assistance is required Updater This app is used to update CellDrop software and firmware to the most current available versions. Updates may be downloaded using the Updater app when connected to Ethernet or Wifi. An Ethernet connection is recommended for software updates. -
Page 28: Software Errors
Pop-up messages will provide guidance for software-based errors or invalid user actions. In most cases, simply re-launching the app will allow the user to continue to make measurements. Update the software using the Updater app if experiencing a reoccurring software error. Contact DeNovix Customer Support at 302-442-6911 or info@denovix.com if additional help is required. - Page 29 Disclaimer All information in this document is for reference purposes only. DeNovix Inc. makes no claims that this document is complete or error-free and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might result from any use of this document.
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