Table of Contents
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Table of Contents
Related Manuals for DeNovix CellDrop
Summary of Contents for DeNovix CellDrop
- Page 1 rev MAR-27-2019...
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Page 2: Table Of Contents
1. Introduction Product Technology Key Features Optics 2. Set-up and Safety 3. Quick Guides Basic Procedure Best Practices Software Icons and Buttons Top Gray Action Bar Chamber Height Options Data Storage 4. Count Apps Brightfield App Trypan Blue App AO App PI App AO/PI App GFP App... -
Page 3: Introduction
The pre-configured app settings are factory optimized for ease of use. The software allows the user to customize settings when needed to meet lab specific workflow preferences. The CellDrop uses three chamber heights to enable cell counts over a wide dynamic concentration range according to specifications. This innovative and patent-pending technology along with user-defined protocols allows users the flexibility required for today’s cell culture workflows. -
Page 4: Set-Up And Safety
Instrument Connectivity The CellDrop comes equipped with one Ethernet, one HDMI and three USB ports. The USB ports are used to facilitate data transfer to a FAT32 formatted USB storage device, and support the use of an Opticon OPI 3601 USB barcode reader. USB mouse and keyboard devices may also be used with CellDrop Series... - Page 5 Cautions DO NOT REMOVE COVER: No operator serviceable components inside. Refer servicing to qualified personnel. CellDrop cell counters are designed for indoor use under the following conditions: Temperature: 15˚ to 35˚ C Humidity: 35 to 65 % Use only the power supply provided with the instrument. Use of the instrument in any manner not specified by the manufacturer may impair the protection provided by the supplied power cord and power supply.
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Page 6: Quick Guides
Avoid introducing air bubbles into the sample solution when mixing or dispensing samples. • Refer to DeNovix assay protocols or other manufacturer guidelines for cell dyes such as Acridine Orange, Propidium Iodide or Fluorescein Diacetate for assay specific instructions. •... -
Page 7: Software Icons And Buttons
Software Icons and Buttons Home Select All Overflow Menu Edit Increase / Decrease Count Copy Export Exposure Delete Clear Selected Focus Expand / Collapse Information Calculator Notifications avorite Channel View Selectors Brightfield/ Green Brightfield Overlay Green Fluorescence Brightfield/ Red Overlay Red Fluorescence Green/ Red Overlay Brightfield/ Green/Red... -
Page 8: Chamber Height Options
Should the memory approach capacity the user will be alerted. Data may be deleted from the CellDrop at any time from the Data App. If the limits are exceeded, old data will be automatically deleted on a first-in, first-out basis. -
Page 9: Count Apps
Trypan Blue Concentration and Exposure Setting Any brand of trypan dye can be used on the CellDrop but the concentration must be either 0.4%, which is the common concentration of the dye sold by many vendors, or 0.2% trypan dye if the user chooses to dilute the dye. -
Page 10: Ao/Pi App
preference. In aqueous solution, the dye has excitation/emission maxima of 493/636 nm. When bound, the excitation/emission maxima shifts to 535/617 nm respectively. Calculation: % Viability =(Brightfield count / (Brightfield count+ PI count)) *100. AO/PI App The AO/PI App uses green fluorescent images to capture live nucleated cell counts and red fluorescent images for counting dead cells. -
Page 11: Count Tab Screen
• Trypan Blue Threshold: The brightfield channel on the CellDrop captures a monochrome image so the darker cells stained with Trypan Blue have a lower intensity. Therefore, the trypan blue threshold setting is the intensity value (scale of 1-100) at which anything below is counted as a dead cell. -
Page 12: Image Controls
CellDrop BF units only include the brightfield channel. • Zoom Controls: All preview and result images on the CellDrop support zoom and pan capabilities. Use two fingers to zoom in or out. Tap and drag the image to move it around. -
Page 13: Results Tab Views
6. Results Tab Views The Results tab includes 6 views, which enable the user to review all sample count data made in the current measurement session. Blue Action Bar • View Drop-Down Menu: This feature is used to navigate between the various Results tab views. The Default selection is Single Sample view. -
Page 14: Single Sample Results View
Single Sample Results View • Sample Number: The arrows on either side of the sample number are used to switch between samples measured in the current session. • Sample Name: This is an editable field. Changes will overwrite the information stored in the database and will be applied to all lists referencing the sample. - Page 15 • GFP+ Cell Count: Available for the GFP app only. Without cell size distribution • Cluster #: Available for all apps. Displays count of cell size clusters. • % Viability: Available for Trypan Blue, PI, AO/PI and Yeast apps.
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Page 16: Export And Print Options
Note: To open an exported .csv file on a computer, the DeNovix instrument and the computer must both be set to use either period or comma number formats. The CellDrop number format is determined by the language selected using the Settings app. -
Page 17: Utility Apps
8. Utility Apps Accounts App User accounts manage user-defined protocols and default settings within specific apps. Accounts are also used as a means of segregating data when performing searches in the Data app. App Structure Accounts are listed in the left pane of the screen. A menu of app function options is presented in the action bar, where the selected option is highlighted with a blue background. -
Page 18: Data App
Features and functions previously described for the Results tab screens apply to the corresponding Data app screens. Export • All data is automatically saved to the CellDrop onboard computer database at the time of measurement. • The Export Selected function available from any Results tab screen Overflow menu enables sample data to be emailed, sent to a network folder or saved to a FAT32 formatted USB flash drive. -
Page 19: Count Codes App
• The storage capability of the CellDrop is a minimum of 120 GB of data. When the storage capacity has been reached the software uses a first in/first out paradigm for remaining sample data. The Delete function should be used as an infrequent method of removing a large amount of data from the CellDrop database. -
Page 20: Timer App
Timer App This feature is not yet implemented and will be included in a future software release. Settings App The Settings app may be found on the second Home screen page. Only administrator accounts may access and change settings. The app provides access to the following functions: System Info •... -
Page 21: Updater App
Refer to your local IT support group if additional assistance is required Updater App This app is used to update CellDrop software and firmware to the most current available versions. Updates may be downloaded using the Updater app when connected to Ethernet or Wifi. An Ethernet connection is recommended for software updates. -
Page 22: Troubleshooting And Maintenance
Pop-up messages will provide guidance for software-based errors or invalid user actions. In most cases, simply re-launching the app will allow the user to continue to make measurements. Update the software using the Updater app if experiencing a reoccurring software error. Contact DeNovix Customer Support at 302-442-6911 or info@denovix.com if additional help is required. - Page 23 Tap the home button to exit. Solvent Compatibility The CellDrop are compatible with most solvents typically used in life science laboratories. Exception: Do not use Hydrofluoric Acid (HF) as the fluoride ion will etch the surfaces of the...
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Page 24: Customer Support
Please include the serial number of your instrument when contacting Customer Support by email. If sending Customer Support a screen capture or a report file using the CellDrop email feature include your name, institute/company, phone number and return email address. Please note responses will not be sent directly to the email account used by the instrument.
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