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- Page 1 Te mp era tur Li d Pl at e 42 .1 I 38 .0 42 .0 S[ 38 .0 ] Ru n Se tu p N. C. 12130 ibidi Heating System 4 Slides – Silver Line .com Version 1.0...
- Page 2 Heating System 4 Slides – Silver Line Version 1.0 (August 1, 2022) Instruction Manual...
- Page 3 Heating System 4 Slides – Silver Line Contact ibidi GmbH Lochhamer Schlag 11 82166 Gr¨ a felfing Germany Phone: +49 89 / 520 46 17 - 0 Fax: +49 89 / 520 46 17 - 59 E-mail: info@ibidi.de Internet: ibidi.com...
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Page 4: Table Of Contents
3 Principle 4 Equipment 4.1 Components of the ibidi Heating System 4 Slides ..... . 4.2 Stage Top Incubator Overview . - Page 5 ..... . 5.2 Start Operation of the ibidi Heating System 4 Slides ....
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Page 6: Preamble
1 Preamble 1.1 Introduction This manual is your guide to using the ibidi Heating System 4 Slides – Silver Line for cell culture experiments on an optical microscope. It instructs first-time users on how to use the instrument, and serves as a reference for experienced users. -
Page 7: Nomenclature
Where adequate, the ibidi Heating System 4 Slides – Silver Line is hereafter abbreviated and referred to as ibidi Heating System 4 Slides in body text and figure captions. The ibidi Temperature Controller – Silver Line is hereafter abbreviated and referred to as Temperature Controller, where adequate. - Page 8 Heating System 4 Slides – Silver Line Table 1: (continued) Operating altitude max. 2000 m (atmospheric pressure 800–1060 hPa/11.6–15.4 psi) Storage conditions -5–50°C/23–122°F, humidity 60% relative humidity (RH) Outer Dimensions and Characteristics of the Components ibidi Temperaure Controller –...
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Page 9: Disclaimer
Holder for standard multiwell plates 1.5 Disclaimer • ibidi shall not be held liable, either directly or indirectly, for any damage incurred as a result of product use. • The contents of this manual are subject to change without notice for product improvement. - Page 10 • Avoid vibrations from vacuum pumps, centrifuges, electric motors, processing equipment, and machine tools. • Avoid dust and corrosive gas. Do not install the ibidi Heating System 4 Slides where it could be exposed to high levels of dust or to outside air or ventilation outlets.
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Page 11: Limited Warranty
1.8 Transporting the ibidi Heating System 4 Slides The weight of the ibidi Temperature Controller – Silver Line is approx. 1.75 kg/3.86 lbs. Moving the ibidi Temperature Controller – Silver Line during operation can pose a risk of personal injury or damage to the instrument. -
Page 12: Repairing The Ibidi Heating System 4 Slides
– Do not try to repair the ibidi Heating System 4 Slides by yourself. Disas- sembly of the ibidi Heating System 4 Slides is not allowed. Disassembly poses a risk of personal injury or damage to the devices. Contact ibidi technical support if there is a need to disassemble a device. -
Page 13: Regulatory Statement
Heating System 4 Slides – Silver Line 1.11 Regulatory Statement EG-Konformitätserklärung EC Declaration of Conformity Wir / We ibidi GmbH Lochhamer Schlag 11 D-82166 Gräfelfing erklären hiermit die Übereinstimmung des genannten Produktes mit der Richtlinie 2014/35/EU - Niederspannungsrichtlinie und mit der Richtlinie 2014/30/EU über die Elektromagnetische Verträglichkeit. -
Page 14: Intended Use
Heating System 4 Slides – Silver Line 2 Intended Use The ibidi Heating System 4 Slides – Silver Line is a stage top incubator for live cell imaging that fits onto inverted microscope stages with a mounting frame for multiwell plates. The ibidi Heating System 4 Slides, consisting of an Incubation Chamber with Heated Plate and Heated Lid and a Tem- perature Controller, provides a platform for keeping ibidi µ-Slides at a constant temperature during... - Page 15 Feedback control Figure 2: Schematic view of the ibidi Stage Top Incubation System, combining the Heating System with the Gas Incubation System. RH: relative humidity. In combination with the Heating System, it supplies complete incubator conditions for your live cell imaging directly on the microscope stage.
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Page 16: Equipment
Heating System 4 Slides – Silver Line 4 Equipment This section provides a brief overview over all parts of the ibidi Heating System 4 Slides including a description of the characteristics. 4.1 Components of the ibidi Heating System 4 Slides The components of the ibidi Heating System 4 Slides are listed below. -
Page 17: Stage Top Incubator Overview
4.2 Stage Top Incubator Overview The parts of the ibidi Heating System 4 Slides are set up as a stage top incubator as shown in Figure 4. The ibidi Heating System 4 Slides can be combined with the ibidi Gas Incubation System – Silver... -
Page 18: Ibidi Temperature Controller - Silver Line
4.3 ibidi Temperature Controller – Silver Line The ibidi Temperature Controller – Silver Line is designed to precisely operate the different ibidi Silver Line heating devices, including the Incubation Chamber 4 Slides – Silver Line. In total, 6 channels are available, each channel accommodating one heated component (e.g., the Heated Lid and Heated Plate of the Incubation Chamber 4 Slides). -
Page 19: Heated Plate And Magnetic Holder
4.4 Heated Plate and Magnetic Holder The Heated Plate provides the base for the ibidi Heating System 4 Slides. It fits into any microscope stage with a mounting frame for multiwell plates. The Heated Plate offers four cavities to mount µ- Slides for parallel observation on the microscope. -
Page 20: Heated Lid
26 mm. The upper glass part of the Heated Lid is heated. The electrical cable is connected to the ibidi Tem- perature Controller – Silver Line. The Heated Lid fits exactly and securely onto the Heated Plate. -
Page 21: Cable Clip For Optional Use
Heating System 4 Slides – Silver Line 4.6 Cable Clip for Optional Use CAUTION – ibidi GmbH does not accept any liability for damages resulting from the use of the cable clip on your microscope. Please check with your microscope provider and/or your institution’s technical support if using the cable clip on the microscope stage... -
Page 22: Operation
Heating System 4 Slides – Silver Line 5 Operation Before starting an experiment, check that the ibidi Heating System 4 Slides fits on your microscope stage, and that your cell culture vessels are compatible with the Holder. Connect all parts (Section 5.1) and perform a temperature adjustment as explained in Section 5.4. -
Page 23: Start Operation Of The Ibidi Heating System 4 Slides
5.2 Start Operation of the ibidi Heating System 4 Slides The ibidi Temperature Controller – Silver Line is switched on by the dip switch at the back. Make sure the display shows “RUN” (see Section 5.3.1). The system immediately starts heating up the heated devices (Heated Plate, Heated Lid). -
Page 24: Run/Stop Mode
Depending on the heated components used, different connector ports (output channels) at the back of the ibidi Temperature Controller – Silver Line can be used (Table 4). Accordingly, the information of one, two, or three heated components with the designations Plate, Lid, Glass or Obj is shown per display. -
Page 25: Select Temperature Sensor
“Setup” entry and pressing the round button. The mode dialog will open. Mode Each channel of the ibidi Temperature Controller – Silver Line can be set to an “On” or “Off” state. For standard operation all channels must be turned on. -
Page 26: Temperature Adjustment In The Sample
Figure 19: Info display. 5.4 Temperature Adjustment in the Sample The ibidi Temperature Controller – Silver Line controls the temperature of the actively heated com- ponents (Heated Lid and Heated Plate). The temperature in the sample results from the interaction of all heated parts with the supporting microscopy stage and the sample. - Page 27 2. Connect the external temperature sensor (thermocouple type K) to the plug at the back of the ibidi Temperature Controller – Silver Line. The connected external temperature sensor will appear in the display. 3. Fill the provided culture vessel (µ-Slide 8 Well high) with water or medium. Volumes should be the same amount you need for your experiments.
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Page 28: Sample Preparation
Heating System 4 Slides – Silver Line 5.5 Sample Preparation Check that the cell cultureware you intend to use fits into Heated Plate of the ibidi Heating System 4 Slides. Prepare the cells according to your protocol and place the multiwell plate into the Heated Plate mounted on the microscope stage. -
Page 29: Incucontrol Software
Heating System 4 Slides – Silver Line 6 IncuControl Software The ibidi Temperature Controller – Silver Line has a USB interface for computer control and data logging. For this purpose, ibidi provides the IncuControl software that comes with the controller or... -
Page 30: Maintenance
(e.g., Barrycidal 36 or Pharmaci- dal). All parts of the ibidi Heating System 4 Slides can be cleaned from the outside. We recommend using ultrapure water for cleaning. Fingerprints on the Heated Lid can be removed using isopropanol (70%) or lens/eyeglass cleaning wipes. -
Page 31: Troubleshooting
Depending on the incubating conditions, small volumes might evaporate quickly, especially during long-term experiments. If you have an actively controlled humidifying device (e.g., ibidi Gas Incu- bation System), increase the set value for relative humidity. Additionally, we suggest using silicone oil (e.g., Anti-Evaporation Oil, ibidi, 50051) to decrease evaporation. - Page 32 GmbH Lochhamer Schlag 11 82166 Gräfelfing Germany Toll free within Germany: Phone: 0800 / 00 11 11 28 Fax: 0800 / 00 11 11 29 International calls: Phone: +49 89 / 520 46 17 - 0 Fax: +49 89 / 520 46 17 - 59 E-Mail: info@ibidi.com...
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