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Summary of Contents for ibidi Universal Fit 10918
- Page 1 Version 2.2 Pl at e Gl as s Li d N. C. 37 .0 I 42 .1 37 .0 37 .0 S[ 42 .0 ] Ru n Se tu p N. C. 10918 ibidi Heating System, Universal Fit for 1 Chamber...
- Page 2 Heating System, Universal Fit Version 2.2 (May 23, 2019) Instruction Manual...
- Page 3 Heating System, Universal Fit Contact ibidi GmbH Lochhamer Schlag 11 82166 Gr¨ a felfing Germany Phone: +49 89 / 520 46 17 - 0 Fax: +49 89 / 520 46 17 - 59 E-mail: info@ibidi.de Internet: ibidi.com Instruction Manual...
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Page 4: Table Of Contents
2 Intended Use 3 Principle 4 Equipment 4.1 Components of the ibidi Heating System, Universal Fit ....4.2 Combination Options ........ - Page 5 ......5.2 Start the ibidi Heating System, Universal Fit ......
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Page 6: Preamble
1 Preamble 1.1 Introduction This manual is your guide to using the ibidi Heating System, Universal Fit for cell culture experi- ments on an optical microscope. It instructs first-time users how to use the instrument, and serves as a reference for experienced users. -
Page 7: Nomenclature
Heated Lid Heated Plate 1.4 Specifications Only operate the Heating System in the range of the specifications given below: Table 1 – Specifications of the ibidi Heating System, Universal Fit Electrical Specifications Power Supply Protection class International protection marking IP 20... - Page 8 Heating System, Universal Fit Table 1 – (continued) Connector to Gas Incubation: Female Luer Lock Heated Plate 12 mm × 85.5 mm × 127.5 mm Observation area: 40 mm × 82 mm Length of cable: 1.5 m Heated Plate...
- Page 9 Heating System, Universal Fit Table 1 – (continued) Microscope Requirements Working distance condenser 26 mm Stage holder Holder for standard multi-well plates Optical Properties Glass Lid Glass Selected HQ Floatglass Thickness of the glass plate 1.1 mm Refractive index glass = 1.520 (588 nm)
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Page 10: Disclaimer
Heating System, Universal Fit 1.5 Disclaimer • ibidi shall not be held liable, either directly or indirectly, for any damage incurred as a result of product use. • The contents of this manual are subject to change without notice for product improvement. -
Page 11: Regulatory Statement
Heating System, Universal Fit • Only ibidi technical staff and technical staff instructed by ibidi are permitted to open and ser- vice the ibidi Heating System, Universal Fit. • The external power supply should not be brought into contact with moisture. If the housing is damaged, the external power supply should not be used. -
Page 12: Limited Warranty
Products manufactured by ibidi, unless otherwise specified, are warrantied for a period of one year from the date of shipment to be free of defects in materials and workmanship. If any defects in the product are found during this warranty period, ibidi will repair or replace the defective part(s) or product free of charge. -
Page 13: Repairing The Ibidi Heating System, Universal Fit
CAUTION Do not try to repair the ibidi Heating System, Universal Fit by yourself. Disassembly of the ibidi Heating System, Universal Fit is not allowed. Disassembly poses a risk of personal injury or damage to the devices. Contact ibidi service personnel if there is a need to disassemble a device. -
Page 14: Intended Use
Heating System, Universal Fit 2 Intended Use The ibidi Heating System, Universal Fit is a stage top incubator for live cell imaging that fits onto inverted microscope stages with a universal mounting frame for multiwell plates. The geometry of the chamber, with a heated glass plate above the sample, provides a platform for keeping microscopy slides and dishes at a constant temperature during an experiment on a microscope. -
Page 15: Equipment
Heating System, Universal Fit 4 Equipment This section provides a brief overview over all parts of the ibidi Heating System, Universal Fit in- cluding a description of the characteristics. 4.1 Components of the ibidi Heating System, Universal Fit The components of the ibidi Heating System, Universal Fit are listed below. -
Page 16: Combination Options
Heating Inserts can be ordered separately. 4.2 Combination Options The parts of the ibidi Heating System, Universal Fit are combined as shown in Figure 3. The Heated Lid and Heated Plate fit on an inverted microscope stage equipped with a universal mounting frame for multiwell plates. -
Page 17: Temperature Controller
Heating System, Universal Fit Figure 3 – Overview of the parts of the ibidi Heating System, Universal Fit with options to combine 4.3 Temperature Controller The Temperature Controller is designed to control the different heated components (four channels available). -
Page 18: Heated Lid
Heating System, Universal Fit Figure 4 – Front view of the Temperature Controller. The LEDs on the front indicate the status of the channels, connection to the power supply, and USB connection (Figure 4). Control LEDs Channel 1-4 LED off... -
Page 19: Heated Plate
Figure 6 – Heated Lid 4.5 Heated Plate The Heated Plate provides the base for the ibidi Heating System, Universal Fit. The lower part of the Heated Plate fits into any microscope stage with a universal mounting frame for multiwell plates. -
Page 20: Heating Inserts
fields are detrimental to your ex- periment. 4.6.1 Heating Insert µ–Slide The Heating Insert µ–Slide fits 75 mm × 25 mm µ–Slides (e.g. all ibidi µ–Slides). Figure 8 – Heating Insert µ–Slide 35mm, high 35mm, low 4.6.2 Heating Insert µ–Dish... - Page 21 Heating System, Universal Fit Figure 9 – Heating Insert µ–Dish To make sure that the µ–Dishes are properly positioned, the holder of the insert must apply a diagonal force in order to snap the dishes into a fixed position. This will leave an inclined gap between the holder and the insert, as shown in Figure 10, but this is intentional and helps to keep the dishes in place.
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Page 22: Operation
Heating System, Universal Fit 5 Operation Before starting an experiment, check that the ibidi Heating System, Universal Fit fits on your micro- scope stage, and that your cell culture vessels are compatible with the Heating Insert. Connect all parts (Section 5.1) and perform a temperature adjustment as explained in Section 5.4. -
Page 23: Start The Ibidi Heating System, Universal Fit
Temperature Controller. The other end is placed in the sample (Section 5.4). 5.2 Start the ibidi Heating System, Universal Fit The Temperature Controller is switched on by the dip–switch on the back. Make sure the display shows ”RUN”... -
Page 24: Run/Stop Mode
Heating System, Universal Fit The display shows the measured (’I’ = instantaneous) and set (’S’ = set) temperatures of all the chan- nels. The cursor position is indicated with square brackets (” ”). You can move the cursor using the ”left”... - Page 25 Heating System, Universal Fit Mode Each channel of the Temperature Controller can be set to an ”On” or ”Off” state. For standard operation all channels must be turned on. <Mode> Setup Channel = 1 Mode = On Return Figure 14 – Mode dialog 1.
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Page 26: Temperature Adjustment In The Sample
5. Place the µ–Dish/µ–Slide in the Insert and put the holder on. 6. Place the Insert in the ibidi Heating System, Universal Fit and close the Heated Lid. 7. After 30 minutes, check the temperature of the external sensor on the display or in the Incu- Control software. -
Page 27: Sample Preparation
• Use of an Objective Heater 5.5 Sample Preparation Check that the cell cultureware you intend to use fits into the Insert of the ibidi Heating System, Universal Fit. Prepare the cells according to your protocol and place the culture vessel in the Heating Insert. The Heating Insert can be removed from the ibidi Heating System, Universal Fit even with the holder in place. -
Page 28: Incucontrol Software
Heating System, Universal Fit 6 IncuControl Software The Temperature Controller has a USB interface for computer control and data logging. For this pur- pose, ibidi provides the IncuControl software that comes with the controller or can be downloaded from the ibidi website. -
Page 29: Maintenance
(e.g. Barrycidal 36 or Pharmacidal). All parts of the ibidi Heating System, Universal Fit can be cleaned from the outside. We recommend using ultrapure water for cleaning. Fingerprints on the Heated Lid can be removed using isopropanol (70%) or lens/eyeglass cleaning wipes. -
Page 30: Troubleshooting
Depending on the incubating conditions, small volumes might evaporate quickly, especially during long-term experiments. If you have an actively controlled humidifying device (e.g. ibidi Gas Incuba- tion System), increase the set value for relative humidity. Additionally, we suggest using silicone oil (e.g. - Page 31 Heating System, Universal Fit Instruction Manual Version 2.2 (May 23, 2019)
- Page 32 GmbH Lochhamer Schlag 11 82166 Gräfelfing Germany Toll free within Germany: Phone: 0800 / 00 11 11 28 Fax: 0800 / 00 11 11 29 International calls: Phone: +49 89 / 520 46 17 - 0 Fax: +49 89 / 520 46 17 - 59 E-Mail: info@ibidi.com...
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