Table of Contents
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Summary of Contents for ibidi 10929
- Page 1 Version 2.0 Pl at e Gl as s Li d 36 .9 38 .0 I 42 .1 37 .0 38 .0 S[ 42 .0 ] Ru n Se tu p N. C. 10929 ibidi Heating System, Multi-Well Plates, K-Frame www.ibidi.com...
- Page 2 Heating System, Multi-Well Plates, K-Frame Contact ibidi GmbH Lochhamer Schlag 11 82166 Gr¨ a felfing Germany Phone: +49 89 / 520 46 17 - 0 Fax: +49 89 / 520 46 17 - 59 E-mail: info@ibidi.de Internet: ibidi.com Version 2.0 (March 22, 2019)
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Page 3: Table Of Contents
2 Intended Use 3 Principle 4 Equipment 4.1 Components of the ibidi Heating System, Multi-Well Plates, K-Frame ..4.2 Combination Options ........ - Page 4 ......5.2 Start the ibidi Heating System, Multi-Well Plates, K-Frame ....
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Page 5: Preamble
1 Preamble 1.1 Introduction This manual is your guide to using the ibidi Heating System, Multi-Well Plates, K-Frame for cell culture experiments on an optical microscope. It instructs first-time users how to use the instrument, and serves as a reference for experienced users. -
Page 6: Nomenclature
1.3 Nomenclature Temperature Controller Heated Lid Heated Plate with Heated Glass Bottom 1.4 Specifications Table 1 – Specifications of the ibidi Heating System, Multi-Well Plates, K-Frame Electrical Specifications Power Supply Protection class International protection marking (IEC IP 20 60529) Overvoltage category External power supply AC 100 –... - Page 7 1.95 Requirements for Objective Lenses Minimum 1 mm working distance for coverslip bottom plates, e.g., ibidi µ-Plate 96 Well Minimum 2 mm working distance for thick bottom plates The sample focal plane in the K-Frame lies up to 3.5 mm above the normal sample focal plane.
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Page 8: Disclaimer
Heating System, Multi-Well Plates, K-Frame 1.5 Disclaimer • ibidi shall not be held liable, either directly or indirectly, for any damage incurred as a result of product use. • The contents of this manual are subject to change without notice for product improvement. -
Page 9: Regulatory Statement
• Immediately replace damaged cords, plugs, or cables to avoid risk of personal injury or damage to the instrument. • Only ibidi technical staff and technical staff instructed by ibidi are permitted to open and ser- vice the ibidi Heating System, Multi-Well Plates, K-Frame. -
Page 10: Limited Warranty
Products manufactured by ibidi, unless otherwise specified, are warrantied for a period of one year from the date of shipment to be free of defects in materials and workmanship. If any defects in the product are found during this warranty period, ibidi will repair or replace the defective part(s) or product free of charge. -
Page 11: Repairing The Ibidi Heating System, Multi-Well Plates, K-Frame
Do not try to repair the ibidi Heating System, Multi-Well Plates, K-Frame by yourself. Disassem- bly of the ibidi Heating System, Multi-Well Plates, K-Frame is not allowed. Disassembly poses a risk of personal injury or damage to the devices. Contact ibidi service personnel if there is a need to disassemble a device. -
Page 12: Eu Directive Rohs
Category 9, monitoring and control instruments. All of our products fall into either Category 8 or 9, and are currently exempt from the RoHS Directive. Nevertheless, the ibidi Heating System, Multi-Well Plates, K-Frame meets the requirements set forth in the RoHS Directive 2011/65/EC. -
Page 13: Intended Use
Heating System, Multi-Well Plates, K-Frame 2 Intended Use The ibidi Heating System, Multi-Well Plates, K-Frame is a stage top incubator for live cell imaging, that fits onto microscope stages with a mounting frame K opening (110 × 160 mm). The geometry of the chamber with a heated glass plate underneath and also above the sample, provides a platform for keeping multi-well plates at a constant temperature during the experiment on the microscope. -
Page 14: Equipment
Heating System, Multi-Well Plates, K-Frame 4 Equipment The ibidi Heating System, Multi-Well Plates, K-Frame consists of the Heated Lid, Heated Plate with Heated Glass Bottom, and a Temperature Controller. This section gives an overview of the components and their functionality and characteristics. -
Page 15: Combination Options
(optional) control and a defined humidified atmosphere. Detailed information on the Gas mixer unit is given in the Gas Incubation System instructions. Figure 3 – Overview of the parts of the ibidi Heating System, Multi-Well Plates, K-Frame with com- bination options Instruction Manual Version 2.0 (March 22, 2019) -
Page 16: Temperature Controller
Heating System, Multi-Well Plates, K-Frame 4.3 Temperature Controller The Temperature Controller is designed to control the different heated components (four channels available). The front display shows the set values (S) and the current values (I). The settings can be adjusted via the control buttons (Section 5.3). -
Page 17: Heated Lid
Heating System, Multi-Well Plates, K-Frame On/Off switch Tem p ibidi GmbH Am Klopferspitz 19 Sensor D-82152 Martinsried Germany +49(0)89520 46 17 0 www.ibidi.com ibidi Temperature Controller Type: External ibiTC2-001 Serial number: Production date: 11/2018 themperature sensor Pow er 24 V m ax. -
Page 18: Heated Plate
Heating System, Multi-Well Plates, K-Frame 4.5 Heated Plate The Heated Plate provides the base for the ibidi Heating System, Multi-Well Plates, K-Frame. The lower part of the Heated Plate fits into a mechanical or scanning stage with opening for mounting frames K (160 mm ×... -
Page 19: Operation
Heating System, Multi-Well Plates, K-Frame 5 Operation Before starting an experiment, check that the ibidi Heating System, Multi-Well Plates, K-Frame fits on your microscope stage, and that your multi-well plates are compatible to the Heated Plate. Connect all parts (Section 5.1) and perform a temperature adjustment as explained in Section 5.4. -
Page 20: Start The Ibidi Heating System, Multi-Well Plates, K-Frame
Optional: To setup communication with the IncuControl Software, the USB cable must be connected between the Temperature Controller and the computer. 5.2 Start the ibidi Heating System, Multi-Well Plates, K-Frame The Temperature Controller is switched on by the dip–switch on the back. Make sure the display shows ”RUN”... -
Page 21: Run/Stop Mode
Heating System, Multi-Well Plates, K-Frame The display shows the measured (’I’ = instantaneous) and set (’S’ = set) temperatures of all the chan- nels. The cursor position is indicated with square brackets (” ”). You can move the cursor using the ”left”... - Page 22 Heating System, Multi-Well Plates, K-Frame Mode Each channel of the Temperature Controller can be set to an ”On” or ”Off” state. For standard operation all channels must be turned on. <Mode> Setup Channel = 1 Mode = On Return Figure 12 –...
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Page 23: Temperature Adjustment In The Sample
Heated Plate. To ensure that the sample reaches the correct temperature, ibidi recommends a tem- perature adjustment step before starting the first experiment. The temperature adjustment should also be done separately for each chamber type used. -
Page 24: Sample Preparation
Note! Working with high aperture objectives might limit the observation area due to their large diam- eter. In this case ibidi recommends leaving out the outermost rows of wells to avoid the risk of objective collision with the Heated Plate. -
Page 25: Incucontrol Software
Heating System, Multi-Well Plates, K-Frame 6 IncuControl Software The Temperature Controller has a USB interface for computer control and data logging. For this pur- pose, ibidi provides the IncuControl software that comes with the controller or can be downloaded from the ibidi website. -
Page 26: Maintenance
(e.g. Barrycidal 36 or Pharmacidal). All parts of the ibidi Heating System, Multi-Well Plates, K-Frame can be cleaned from the outside. We recommend using ultrapure water for cleaning. Fingerprints on the Heated Lid can be removed using isopropanol (70%) or lens/eyeglass cleaning wipes. -
Page 27: Troubleshooting
Depending on the incubating conditions, small volumes might evaporate quickly, especially during long-term experiments. If you have an actively controlled humidifying device (e.g. ibidi Gas Incuba- tion System), increase the set value for relative humidity. Additionally, we suggest using silicone oil (e.g. - Page 28 Certified ISO 9001:2008, EN ISO 13485:2012 ibidi GmbH Lochhamer Schlag 11 82166 Gräfelfing (München) Germany Toll free within Germany: Phone: 0800 / 00 11 11 28 Fax: 0800 / 00 11 11 29 International calls: Phone: +49 89 / 520 46 17 - 0 Fax: +49 89 / 520 46 17 - 59 E-Mail: info@ibidi.com...
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