The information contained in this document is subject to change without notice. Bruker Daltonics GmbH & Co. KG makes no warranty of any kind with regard to this material, including, but not limited to, the implied warranties of merchantability and fitness for a particular purpose.
Bruker Document History Document History Title: Quick Guide EPSON Perfection V850 Revision: Revision A (July 2021) Document number: 5046693 First revision: July 2021 The following table describes important changes from the previous revision of this document. List of changes No changes: First revision...
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Bruker Table of Contents Table of Contents Legal and Regulatory Notices Document History Manufacturer Table of Contents Overview Site Specifications System Requirements Installation Create a Scan Troubleshooting Service Information Index Quick Guide EPSON Perfection V850 Revision A Page 5 of 23...
1 Overview Bruker Overview This document provides information on how to use the EPSON Perfection V850 Pro scanner and EPSON Scan software for creating pre-measurement scans that can be used for the automatic workflow and SCiLS™ autopilot in Bruker’s flexImaging software.
Bruker 2 Site Specifications Site Specifications EPSON Perfection V850 Pro (Bruker cat #1888343) EPSON Scanner Driver and EPSON Scan Utility v3.9.3.4 (download from EPSON). IntelliSlide (Bruker cat #1868957) with sample. Quick Guide EPSON Perfection V850 Revision A Page 7 of 23...
3 System Requirements Bruker System Requirements CPU: Pentium Core processor equivalent or better, clock frequency 1.6 GHz or more for satisfying data handling (Recommended: dual core workstation or better for acquisition). Hard disk: at least 12 GB of free disk space Main Memory: minimum 8 GB RAM.
Bruker 4 Installation Installation You need administrator privileges for all installation/uninstallation tasks. Please make sure that the scanner device is connected to the computer where this software will be installed. 1. Download the Scanner Driver and EPSON Scan Utility v3.9.3.4 from EPSON 2.
5 Create a Scan Bruker Create a Scan When using the EPSON Perfection V850 Pro to create high resolution scans of your slides, it is important to take note of the following: Create your optical pre-measurement scan before matrix coating.
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Bruker 5 Create a Scan Figure 1-1 EPSON Perfection V850 with sample film adapter on scanning surface Note Please note the alignment of the white triangles with each other at the right edge of the adapter and the height adjustment setting of the adapter (red boxes and arrows).
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5 Create a Scan Bruker 6. In the EPSON Scan software, make sure you have applied the following settings: Mode: Professional Mode Document type: Film (with Film Holder) Image Type: 24-bit Color Resolution: 3200 dpi Scale: 100% Figure 1-2 EPSON Scan software showing the recommended scan settings...
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Bruker 5 Create a Scan 7. Click the “Preview” button on the bottom left Creation of a low-resolution preview scan of your sample starts. Two new windows open: Progress window showing a green progress bar. Preview window showing the preview image.
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5 Create a Scan Bruker Figure 1-3 EPSON Scan software showing low resolution preview in Prescan Image tab, and dotted line box indicating scan area 10. Click “Zoom” in the Prescan window to check that the ROI defined for the high- resolution scan encompasses the entire IntelliSlide without cropping any of the inscribed features.
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Bruker 5 Create a Scan Figure 1-4 Zoomed view of the defined ROI for the high-resolution scan Note If the dotted box crops some of the inscribed features, hold down the left mouse button to move the dotted box so that the features are included in the area.
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5 Create a Scan Bruker Figure 1-5 Histogram adjustment settings Note The adjusted histogram settings are important for the automatic tissue recognition during the SCiLS™ autopilot. 14. Click the “Scan” button to create a high-resolution scan of the area defined in the Prescan tab.
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The folder in which your image is saved should open immediately after the scan is complete. The scan output from the EPSON Perfection V850 Pro will be a mirror-image of the slide 17. Rotate the image (horizontal flip) before use via Windows applications such as...
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5 Create a Scan Bruker Figure 1-7 Location of ‘flip’ function in Windows Photos Figure 1-8 Correct orientation of a scanned IntelliSlide (without sample) Page 18 of 23 Quick Guide EPSON Perfection V850 Revision A...
6 Troubleshooting Troubleshooting Incorrect definition of the high- resolution scan ROI can produce scans that are incompatible with the automated workflows in Bruker’s flexImaging software. The following are examples of scans resulting from incorrect ROI definition. Figure 1-9 Scan created using ROI larger than IntelliSlide...
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6 Troubleshooting Bruker Figure 1-10 Scan created using ROI smaller than IntelliSlide, resulting in cropping of barcode, serial number and Bruker logo Page 20 of 23 Quick Guide EPSON Perfection V850 Revision A...
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Bruker 6 Troubleshooting Figure 1-11 Scan created using ROI smaller than IntelliSlide, resulting in cropping of barcode and teach marks Quick Guide EPSON Perfection V850 Revision A Page 21 of 23...
7 Service Information Bruker Service Information For questions, please contact service.bdal.de@bruker.com Page 22 of 23 Quick Guide EPSON Perfection V850 Revision A...
Bruker Index Index Create a Scan 10 Installation 9 Overview 6 Service Information 22 Site Specifications 7 System Requirements 8 Troubleshooting 19 Quick Guide EPSON Perfection V850 Revision A Page 23 of 23...
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