TANBead Maelstrom 4800 LH User Manual

TANBead Maelstrom 4800 LH User Manual

Nucleic acid extractor (non-sterile)

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"TANBead" Nucleic Acid Extractor
(Non-Sterile)
Maelstrom 4800 LH
User Manual

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Summary of Contents for TANBead Maelstrom 4800 LH

  • Page 1 "TANBead” Nucleic Acid Extractor (Non-Sterile) Maelstrom 4800 LH User Manual...
  • Page 2: Table Of Contents

    Table of Contents 1. Introduction................3 2. Overview ................7 3. Installation ................10 4. Get started ................10 5. Edit program ............... 13 6. Communication ..............20 7. Product Performance…………………………………………….23 8. Cleaning and maintenance ..........25 9. Disposal ................25 10.
  • Page 3 About this manual The label on the instrument, the User Manual, and other packaging material may contain the following symbols: Catalog number Non-Sterile Serial number Temp Limit (Operating) Manufacturer Humidity Limit (Operating) The date of manufacture Biologic Risks Keep Away From Sunlight Keep Dry Consult Instructions for Use The CE mark...
  • Page 4: Introduction

    1. Introduction Maelstrom 4800 LH is an automated nucleic acid platform designed for high- throughput applications. Specialized spin tips enable super mixing efficiency of magnetic beads and larger processing volume. With intuitive interface and flexible program, Maelstrom 4800 LH can boost laboratory productivity by transforming routine operations into a walk-away solution.
  • Page 5 Environmental Requirements To avoid shortening of the lifespan of the instrument, use Maelstrom 4800 LH in a location that meets the following criteria: Choose a location that has good air circulation.  Place Maelstrom 4800 LH on the table that can bear the weight of ...
  • Page 6 (gloves, safety goggles, lab coat, etc.) for handling and disposing of biohazardous materials. Under normal condition, sound pressure level from Maelstrom 4800 LH  does not exceed 80 dB and cause a hazard. Please contact technical support in case of higher sound pressure level.
  • Page 7 Equipment list Item Power cord A plate of spin tips Magnetic separator User Manual...
  • Page 8: Overview

    2. Overview  Specifications Model: Maelstrom 4800 LH Weight: 26 kg Dimensions: 29 x 55 x 28.5 cm Controller dimensions: 32 x 30 x 14 cm Power supply: 100-240 V Capacity: 48 samples per run Process. volume: 50 μl – 1600 μl...
  • Page 9  Side view of extraction platform:  Shield plate:...
  • Page 10  Regulators of the sensors for detecting falling spin tip:  Control panel:  Buttons on control panel:...
  • Page 11: Installation

    3. Installation 1. Controller and extraction platform are to be connected by 25-pin power cable and 25-pin signal line connectors. 2. Connect electrical inlet of controller to an electrical outlet. 4. Get started Turn on the main power switch (located on the back of controller) to the (on) position.
  • Page 12 Temperature UV LAMP Confirm the Selected Program Mount the Spin Tips...
  • Page 13 Eject Spin Tips STATUS: RUNNING STATUS: PAUSE STATUS: PAUSE ? STATUS:...
  • Page 14: Edit Program

    AutoMag program. 5.1 Connection (1) Switch on Maelstrom 4800 LH. (2) Connect (A) of transmission cable to the Maelstrom 4800 LH controller. (Fig 2) (3) Connect (B) to the USB port of PC. (Fig 3) Fig 1: RS232 transmission cable...
  • Page 15 5.2 To start AutoMag software (A) Enter letters as User name. (maximum characters: 10) (B) Enter “0” as a default Password. (maximum characters: 8) (C) COM port: In drop-down menu, select the used USB port. NOTE: The used USB port can be checked in window system control /device manage/port.
  • Page 16 5.3 Edit a program by AutoMag software Parameter Name Function Program No File name (00~99) Program Name Program name (maximum characters: 15) Plate Plate position (1-8, 0 is program end.) Mount/Eject spin tips (ON=Mount, OFF=Eject) Warm up temperature (0-150℃) Pre-Heat Heating Execute program temperature (0-150℃) Mixing(M)
  • Page 17  NOTE 1:  NOTE 2: Definition of plate number...
  • Page 18 Sample programs: SHORTTEST STEP1: The extraction head will mount spin tips on the left half part of a spin tip rack. After mounting the spin tips, the user can replace the spin tip rack with a reagent deep well plate. After placement of reagent deep well plate, press “Pause”...
  • Page 19 5.4 Instructions of keys File: New: Create a new file Open: Open an old file Save: Save a file End: Close the file Edit: Cut: You can cut a whole row of data when you select a certain row. You can also cut data of selected cells.
  • Page 20 5.5 Transmission of programs between the PC and M4800 LH controller During program transmission, please do not switch off the machine and  the PC.  Import: Transfer the program from the machine to the PC (A).   Export: Transfer the programs from the PC to the machine (B). ...
  • Page 21: Communication

    (B) Example of program transmission (export): Programs saved in the same folder can be exported to the controller: Use export to transfer the program from the PC to the controller.   Set 1 ~ 1 and press “Export” button to transfer program#1 to the ...
  • Page 22 6.2 Communication between M4800 LH and Liquid Handling System...
  • Page 23 6.4 Communication-CRC (Cyclical Redundancy Check)
  • Page 24: Product Performance

    7. Product Performance Consistency The consistency of extracted lysates on the same machine and different machines (within-run precision) is the variability of the results from well to well. The coefficient of variation of nucleic acid extraction concentration is less than 5%. It is in the range of acceptable statistical analysis. ▓...
  • Page 25 Reproducibility The reproducibility of extracted lysates on the same machine (run-to-run precision) is the variability of the results from repeated measurements. The coefficient of variation of nucleic acid extraction concentration is less than 5%. It is in the range of acceptable statistical analysis.
  • Page 26: Cleaning And Maintenance

    If this happens, the magnetic rod needs to be cleaned. To clean the magnetic rods, wipe with a soft cloth dampened with 70%  ethanol. If the magnetic rods cannot be cleaned, please contact TANBead (Tel:  +886-3-3167568) for technical assistance. 9. Disposal In case the product is to be disposed of, the relevant legal regulations are to be observed.
  • Page 27: Patent

    Patent Patents USA: US09616398B2 EP2937136 Canada: CA2862946 Japan: JP6151735 Korea: 10-1696517 WIPO: WO2016127292 China: CN104971638B Taiwan: I526245 11. Appendix Transport ■ To avoid damaging the linear side through the transport, please use EPE foam buffers to fix the mechanism before transporting.
  • Page 28  Manufacturer:Taiwan Advanced Nanotech Inc.  Manufacturer Add:4F., No. 188, Wenhe Rd., Guishan Dist., Taoyuan City 333, Taiwan (R. O. C.)  Manufacturer Tel: +886-3-3167568 http://www.tanbead.com/en  Contact details : mdi Europa GmbH  Address : Langenhagener Street 71, D-30855 Langenhagen, Germany...

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