TANBead Maelstrom 8 Autostage User Manual
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TANBead Maelstrom 8 Autostage User Manual

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Magnetic Rotary Mixer
Maelstrom 8-channel Handler

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  • Page 1 Magnetic Rotary Mixer Maelstrom 8-channel Handler...

  • Page 2: Table Of Contents

    Table of Contents About This Manual ......................1 Introduction ......................2 Equipment List ....................4 Overview ......................5 Environmental Requirements ................7 Safety Instructions and Guidelines ..............7 Before Usage ....................... 8 Switch on or off ....................9 Main Page ......................10 Functional Keys ....................

  • Page 3: About This Manual

    About This Manual The label on the instrument, the User manual, and other packaging material may contain the following symbols:...

  • Page 4: Introduction

    1 Introduction Maelstrom 8-channel Handler (the M8-H) is a magnetic bead handling equipment with high speed stirring function, which can spin up to 3000 rpm. With eight 2500 gauss magnetic rods, intuitive interface and simple operation, the M8-H can accomplish any application of magnetics beads. Please read this manual carefully before usage.
  • Page 5 Maelstrom 8 Autostage (the M8-H Autostage). This model offers a fully automated nucleic acid platform. ▪ The M8-H can be used in combination with TANBead Nucleic Acid Extraction Kit to extract DNA/RNA from specimen. ▪ The M8-H can handle magnetic beads for purification of nucleic acid.

  • Page 6: Equipment List

    2 Equipment List...

  • Page 7: Overview

    3 Overview...

  • Page 9: Environmental Requirements

    TANBead and its agents are not responsible for the consequences caused by the reasons above.

  • Page 10: Before Usage

    The battery level and the requiring time for a full charge may vary under different circumstances. The battery level will decrease slowly even when the M8-H is not being used. Do not disassemble the M8-H. For assistance, please contact your local distributor or manufacturer (TANBead). Fig.1 Fig.2 Fig.4...

  • Page 11: Switch On Or Off

    7 Switch on or off Press menu button to switch on the M8-H. To turn off, press menu button for 3 seconds. Press menu button (M) Press menu button (M) for 3 seconds...

  • Page 12: Main Page

    8 Main Page From Main page, user can choose to operate the M8-H in Guide mode or Manual mode. To pre-set or edit program, please enter edit page. To set up system beep sound, auto-off, time and date, please enter the setting page. Further system information can be found in setting page.

  • Page 13: Functional Keys

    9 Functional Keys Menu Button Press menu button to turn on the M8-H. To turn it off, press the menu button for 3 seconds. User can return to main page without saving changes by pressing menu button twice. In Guide mode, user can exit program by pressing menu button twice. RETURN Press menu button (M) Press twice...
  • Page 14 The Click Wheel In manual mode, use up or down key to increase or reduce the spin speed. Use left or right key to change the spin direction. In Edit page, use left and right keys to move to each factor. Use up and down keys to adjust the factor.

  • Page 15: Guide Mode

    10 Guide Mode Follow the instructions on the screen, and press enter/mixing trigger to proceed. There are pre-installed programs in the M8-H. Use click wheel and enter/mixing trigger to select. About editing the program Please refer to the instructions of edit page for further editing.
  • Page 16 Mixing Mount the spin tips to the M8-H, press enter/mixing trigger to start mixing. Press enter/mixing trigger again to stop mixing. Collection When mixing stopped, raise the M8-H off the wells, and press rod button to extend magnetic rods. After the rods are completely extended, slowly insert the M8-H with the spin tips into the well.

  • Page 17: Manual Mode

    11 Manual Mode User can perform each operation separately in manual mode. Mixing 1. Mount the spin tips to the M8-H. 2. Insert the M8-H to the bottom of wells and press enter/mixing trigger to start mixing. 3. Press enter/mixing trigger to stop mixing. 4.

  • Page 18: Edit Page

    12 Edit Page User can edit, copy, rename or delete programs in edit page. In the absence of the M8-H Autostage, the program serves as a guide to lead user through the operation in guide mode. NOTE: The status of “Stand Connection” should be set as “Disable” (The path of Stand Connection: Setting →...
  • Page 19 Program name:  Maximum 14 characters.  Press rod button (R) to switch between input keys (abc, ABC, 123, @, DEL, INS)  abc: a-z  ABC: A-Z  123: 0-9  - ( )  DEL: press enter/mixing trigger to delete a character ...
  • Page 20 Stir: Frequency of rotation change.  Input value from 0-990.  Number “20” means the spin direction changes every 20 seconds. Procedure: Press enter/mixing trigger to select the target program In Cap. & Temp. Edit page, user can change the volume of each well and set a temperature for whole program by entering a value and ticking in enable box.

  • Page 21: To Edit The Protocol In Computer

    To edit the protocols in your computer User can edit the protocols in an Excel file. Procedure: Connect the M8-H with a computer by using transmission cable. The program files (“01.csv”) are located in the folder of the M8-H. Open the program file in Excel. Edit and save the file in CSV format.
  • Page 22 Well:  User can choose a well to set the specific action.  When it is connected with the M8-H Autostage, the M8-H can process maximum 12 columns of wells. Mode: Performable actions.  C: Collecting  M: Mixing  V: Vaporing ...

  • Page 23: Setting Page

    13 Setting Page User can obtain the system information and adjust parameters in setting page. System Info This page shows the version of hardware and software and S/N number of the M8-H. If the detailed setting information before shipping is needed, please contact manufacturer. Time Setting User can use left and right keys of click wheel to move the cursor, and use up and down keys of click wheel to adjust the...
  • Page 24 ) will be shown on the screen. NOTE 2: About Rod offset This value was set by manufacturer (TANBead) before shipping. This value affects the performance of nucleic acid extraction. We DO NOT recommend user to change this value without authorization. TANBead and our distributors are not responsible for any...

  • Page 25: Error Messages

    14 Error Messages Error messages Description Solution Display Alarm 1. Reboot. System image file is 2. If it happens again, please not found during booting. contact technical support. Uncharged, battery Please charge the M8-H with power remains less cable or mini USB. than 10% In the boot state, the voltage is less than...
  • Page 26 Error messages Description Solution Display Alarm 1. Reboot. Motor module is not 2. If it happens again, please detected. contact technical support. 1. Reboot. 2. If it happens again, please DC motor is blocked. contact technical support. 1. Reboot. Step motor is over 2.

  • Page 27: Cleaning And Maintenance

    To clean the magnetic rods, wipe with a soft cloth dampened with 70% ethanol.  If the magnetic rods cannot be cleaned, please contact your local distributor or manufacturer (TANBead) for technical assistance. 16 Disposal In case the product is to be disposed of, the relevant legal regulations are to be observed.

  • Page 28: Patents

    17 Patents Patents USA: US09616398B2 EP2937136 Canada: CA2862946 Japan: JP6151735 Korea: 10-1696517 WIPO: WO2016127292 China: CN104971638B Taiwan: I526245...

  • Page 29: Accessories

    TANBead Nucleic Acid Extraction System Maelstrom 8 Autostage (non-sterile) Introduction TANBead Nucleic Acid Extraction System Maelstrom 8 Autostage (the M8-H Autostage) provides an efficient and comfortable operating platform for the Maelstrom 8-channel Handler (the M8-H). The M8-H Autostage is a firm stand and a precise arm for the M8-H operations.

  • Page 30: Appendix

    19 Appendix TANBead Nucleic Acid Extraction System Maelstrom 8 Autostage (non-sterile) 19.1 Intended Use: ▪ The M8-H Autostage can be used in combination with the Maelstrom 8 and TANBead’s Nucleic Acid Extraction Kits to extract DNA/RNA from samples. ▪ The M8-H Autostage can hold the Maelstrom 8 for a stable, precise, and automated operation.
  • Page 31 19.2 Equipment List NOTE: The function of the transmission cable combines with charging for the M8-H and software communication between the M8-H and the M8-H Autostage.
  • Page 32 19.3 Overview  Main operation interface  Power input...
  • Page 33 19.4 Environmental Requirements  Operation condition: Temperature: 4-40℃ Humidity: 40-80%  Storage and transportation condition: Temperature: 8-40℃ Humidity: 0-85%  Maximum operating altitude: 2000 m  To avoid shortening the lifespan of the instrument, use the M8-H Autostage in a location that meets the following criteria: Choose a location that has good air circulation.
  • Page 34 Place the 96-well plate on the slider, and checking the relative position. If further assistance is required, please contact your local distributor or manufacturer (TANBead). 19.7 Operating Procedure NOTE: Always to turn on the M8-H first, following by the M8-H Autostage.
  • Page 35 19.8 Powering It is feasible to use AC 100~240 (volts) for the adapter. DC 19 volts is used on the Autostage. Notice: The heater button is the switch for the heated carriage plate; the power button is the switch for the M8-H Autostage (excluding the heating function). 19.9 Standby Mode After resetting or finishing the program, the Autostage will stay on the standby...
  • Page 36 19.10 Heating Mode The temperature of the heated carriage plate can be to set up in the M8-H. The maximum temperature is 70℃. 19.11 Emergency Mode Pushing the emergency stop can stop running the M8-H Autostage immediately and alert with a warning tone. Please follow the steps below for exiting the emergency mode.
  • Page 37 19.12 Elution Setting The M8-H Autostage provides an Elute function to perform elution directly in PCR tubes. With the adaptor of the 8-strip- PCR tube, the elution can be performed in the 8-strip-PCR tube directly. User can switch the height setting for elution in deep well plate (L) or 8-strip-PCR tube (H) by pressing the ELUTE button.
  • Page 38 19.13 Error Messages How to exit Error Messages: Cleaning and Maintenance  If samples or reagents have been spilled, it is required to clean the device immediately with alcohol to avoid damage or contamination of the samples.  Wear gloves and appropriate personal protective equipment. If the device is used with biohazardous materials, dispose of any cleaning materials used in accordance with your institutional guidelines.
  • Page 39 Please follow your institutional and country-specific requirements to handle the disposal of accessories. Device must be decontaminated prior to disposal. Manufacturer Taiwan Advanced Nanotech Inc. No.2, Aly. 12, Ln. 81, Longshou St., Taoyuan District, Taoyuan City 330, Taiwan (R.O. C.) TEL: +886-3-3167568 http://www.tanbead.com/en...
  • Page 40 10F., No.95, Xinpu 6th St., Taoyuan Dist., Taoyuan City 330, Taiwan (R.O.C.) Tel: +886-3-3167568 Fax: +886-3-3173369 V2.0...

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M8-h

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