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Introduction and Principle
Omega Bio-tek's Mag-Bind® Total Pure NGS Kit allows rapid and reliable isolation of PCR
products with high recovery rates. The system combines Omega Bio-tek's proprietary
chemistries with the reversible nucleic acid-binding properties of magnetic beads to
selectively bind DNA fragments 100 bp and larger and eliminate excess nucleotides,
primers, and small, non-targeted amplification products, such as primer dimers. This kit
is designed for both manual and fully automated purification of PCR samples. Purified
PCR fragments can be used for microarrays, automated fluorescent DNA sequencing,
restriction enzyme digestion, and other applications.
The Mag-Bind® Total Pure NGS magnetic particles technology provides a better solution
for nucleic acid purification compared to centrifugation and vacuum-based technologies.
The product can be easily scaled up while providing very user-friendly handling
procedures. If using Mag-Bind® Total Pure NGS for the first time, please read this booklet
to become familiar with the procedures. DNA products are first mixed and bind to Mag-
Bind® Total Pure NGS particles. With two rapid wash steps, trace contaminants such as
nucleotides, primers and small, non-targeted amplification products are removed. Pure
DNA is eluted in Elution Buffer or water. Purified DNA can be directly used in downstream
applications without the need for further purification. The volume of Mag-Bind® Total Pure
NGS can be varied to selectivly bind difference size fragments. Mag-Bind® Total Pure NGS
can be used with next generation sequencing library preparations protocols for reaction
clean-ups at amounts specificed by the library construction manufacturer.
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