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Please use this guide to troubleshoot any problems that may arise. For further assistance,
please contact the technical support staff, toll free, at 1-800-832-8896.
Possible Problems and Suggestions
Problem
Cause
Low PCR product yield
Smaller PCR product size
Ethanol residue
Low yield
Particle loss during the
procedure
DNA remains bound to
beads
Incomplete resuspension
of the beads during
elution
Problem
Cause
Primer
Insufficient wash of the
carryover
particles
Problem
Non-specific
The size of the non-
amplification
specific amplification
products were
products are larger than
not removed
100 bp
Problem
Cause
Salt carryover
Problems in
downstream
applications
Ethanol carryover
Troubleshooting Guide
Solution
Increase the number amplification cycles
for PCR
Small PCR fragments normally give lower
yield.
During the drying step, remove any
liquid from bottom of the well.
Increase magnetization time.
Aspirate slowly.
Increase elution volume.
Vortex or pipet up and down to fully
resuspend the beads.
Solution
Wash the beads one more time with 70%
ethanol.
Solution
Non-specific amplification products
larger than 100 bp are not efficiently
removed from PCR products.
Solution
70% ethanol must be stored at room
temperature.
Ensure the beads are completely dried
before elution.
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