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Mag-Bind® Total Pure NGS - 384-well Plate Protocol
5.
Pipet up and down 5-10 times or vortex for 30 seconds.
6.
Let sit at room temperature for 5 minutes.
7.
Place the plate on a magnetic separation device to magnetize the Mag-Bind®
Total Pure NGS. Let sit at room temperature until the Mag-Bind® Total Pure NGS is
completely cleared from solution.
8.
Aspirate and discard the cleared supernatant. Do not disturb the Mag-Bind® Total
Pure NGS.
9.
Add 30 μL 70% ethanol to each well.
10. Let sit at room temperature for 1 minute. It is not necessary to resuspend the Mag-
Bind® Total Pure NGS.
11. Aspirate and discard the cleared supernatant. Do not disturb the Mag-Bind® Total
Pure NGS.
12. Repeat Steps 9-11 for a second 70% ethanol wash step.
13. Leave the plate on the magnetic separation device for 5 minutes to air dry the Mag-
Bind® Total Pure NGS. Remove any residual liquid with a pipettor.
Note: It is important to dry the Mag-Bind® Total Pure NGS before elution. Residual
ethanol may interfere with downstream applications.
Optional: Incubating the plate at 37°C can speed up evaporation.
14. Remove the plate from magnetic separation device.
15. Add 30 µL Elution Buffer (not provided) to each well.
16. Pipet up and down 20 times or vortex for 30 seconds.
9
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