BRIGHT OTF6000 Manual
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BRIGHT OTF6000 Manual

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  • Page 1 OTF6000 Cryostat Manual Instruments Instruments...

  • Page 2: Table Of Contents

    2.1.9 Settings: Admin Account Receipt of Product 2.1.10 Settings: UV Decontamination 1.1.1 Receipt and unpacking 2.1.11 Settings: Defrost Control 1.1.2 Receipt 2.1.12 Settings: User Management 1.1.3 Unpacking 2.1.13 Settings: Set Clock 1.1.4 Unpacking Procedure 2.1.14 Settings: Event Log Assembly and Installation Bright Instruments...
  • Page 3 Low Temperature Oil 57491 3.1.2 Carbon Dioxide (Gas) Decontamination REFERENCES & DRAWINGS 3.1.3 Carbon Dioxide (Solid) SPECIFICATIONS Figure 1. OTF6000 Cabinet 3.1.4 Freezer Plates Cryostat Figure 2. 5050 Microtome Solid Knife Fitting the Specimen 5050 Microtome Figure 3. 5050 Microtome Solid Knife...

  • Page 4: Safety Warnings

    SAFETY WARNING Low temperatures are present in this equipment. Extreme care should be taken. DO NOT let bare skin come into contact with metal surfaces. Bright Instruments...

  • Page 5: Knives / Blades

    SAFETY WARNING Extremely sharp knives/blades. Use knife/blade guards at all times. Use correct tools for removal and insertion of knives/blades. DO NOT leave knives/blades laying around. Place knives/blades not in use, into box/wallet provided. Bright Instruments...

  • Page 6: Safety Information

    Remember that even used knives are It is recommended the OTF6000 dangers of misuse and/or incorrect handling dangerous – they are still sharp and may is plugged in via an electrical surge of these products.

  • Page 7: Operation

    Operation Accessories When placing object holders in the microtome, Fluids supplied as accessories with Bright when orientating, manipulating or in any way instruments, such as Cryospray 134, placing fingers in a position above the knife Cryo-M-Bed and microtome oil, are strictly for edge - ensure that the handwheel is locked laboratory use only.

  • Page 8: Decontamination Certificates

    Should the instrument or any part of it be returned to Bright Instrument Company Limited, a decontamination certificate must received in a condition that Bright Instrument accompany it, and please note the following: Company Limited, consider to be a potential biological hazard, the instrument or part will a.

  • Page 9: Warranty

    Seller’s liability under this clause shall be in lieu of any warranty or condition implied by law as to the quality or fitness for any particular purpose of such goods. Bright Instruments...

  • Page 10: Introduction

    1. Introduction 1.1 RECEIPT OF PRODUCT Based on the long-established and reliable 1.1.3 UNPACKING OTF cryostat range, the new OTF6000 brings All packing must be carefully removed and 1.1.1 RECEIPT AND UNPACKING Bright cryostats completely up to date. New parts checked against the enclosed packing...

  • Page 11: Assembly And Installation

    1.2.3 FROST ELIMINATION COVER Place the provided Frost Elimination cover onto the Freezer Plate. The cover stops the build up of frost on the Freezer Plate. Freezer Plate Frost Elimination Cover NOTE: Instrument should be placed on a level floor. Bright Instruments...

  • Page 12: Installation And Removal Of

    The use of an industrial, personal lifting belt is Line up white dashes highly recommended. To remove the microtome and shelves repeat on socket and plug all these steps in reverse. Bright Instruments...

  • Page 13: Electrical

    1. Introduction Cont. 1.3 ELECTRICAL It is recommended the OTF6000 1.3.1 SETTLING is plugged in via an electrical surge protection unit. During transit the oil in the compressor will have been subject to movement, so it is important to let the cryostat settle before 1.3.3 ELECTRICAL SAFETY...

  • Page 14: Operating Instructions

    2. Operation Instructions Instruments 2.1 TOUCHSCREEN Chamber Temperature Relevant task / 14.3 warning messages °C 2.1.1 LOCK SCREEN. DECONTAMINATION DUE Cryostat OTF6000 Present Tag To Start Instruments Chamber Temperature Instruments 15.0 °C Chamber Temperature 12.6 °C DEFROSTING Cryostat OTF6000 Present Tag To Start...

  • Page 15: Home Screen Low Spec

    Advance position Rewind position indicator. Turns indicator. Turns red when furthest red when furthest forward. back. Settings Light Off End Session Sign out Turn light on/off Settings Menu Goes green when the microtome head is at start of retraction. Bright Instruments...

  • Page 16: Home Screen High Spec

    (Touch to set target) Live reading of Specimen Temperature. (Touch to set target) Defrost Schedule Defrost Scheduled Confirmed Settings Motor Drive Light Off End Session Sign out Turn light on/off Settings Menu Press to enter Cutting Zone controls Bright Instruments...

  • Page 17: Temperature Settings: Specimen

    Temperature Temperatures Increase (Selecting will Specimen Temperature Soft Target Specimen change both -19.5 Temperature specimen °C Target and chamber temperatures Harder to the preset parameters) Target Specimen Temperature Exit Fatty Return to Home Screen Decrease Target Specimen Temperature Bright Instruments...

  • Page 18: Temperature Settings: Chamber

    Temperature Temperatures Increase (Selecting Chamber Temperature Soft Target Chamber will change -23.0 Temperature both chamber °C Target and specimen temperatures Harder to the preset parameters) Target Chamber Temperature Exit Fatty Return to Home Screen Decrease Target Chamber Temperature Bright Instruments...

  • Page 19: Motor Drive

    (See next page) Rewind position Fast Slow Slow Fast Advance position indicator. Turns Speed Speed Speed Speed indicator. Turns Rewind Rewind Advance Advance Return to red when furthest red when furthest back. forward. Home Screen Bright Instruments...

  • Page 20: Motor Drive: Temps Screen

    Advance position Rewind position indicator. Turns indicator. Turns red when furthest red when furthest forward. back. Settings Light Off End Session Sign out Turn light on/off Settings Menu Goes green when the microtome head is at start of retraction. Bright Instruments...

  • Page 21: Settings: User Account

    Please note that there will be a pause and an extra 5ºC can be achieved in the of 40 to 70 seconds between the compressors cryostats freezing performance. starting in order to reduce current surges. Bright Instruments...

  • Page 22: Settings: Admin Account

    ‘log in’ data Defrost Control Defrost duration Single Dual and time settings Compressor Set Clock Set time and date Exit User Management Return to Home Screen HIGH SPEC ONLY Select machine to run in either single or dual compressor mode. Bright Instruments...

  • Page 23: Settings: Uv Decontamination

    10 hrs 11 hrs 12 hrs Return to Home Screen Once a duration is selected a ‘Decontamination Scheduled’ message will appear on Home Screen. It won’t begin until the Users session has ended and the window is closed. Bright Instruments...

  • Page 24: Settings: Defrost Control

    Triple Defrost Return to Once duration is set. Select single, double or triple defrost by Home Screen touching one of the fixed time buttons. Once selected a ‘Cancel’ button will appear in the top left corner of the screen. Bright Instruments...

  • Page 25: Settings: User Management

    RFID key fob scanner (to the right of the screen) to program the fob for this user. If you Skip Present RFID Tag to assign to Tony Atkins are not using a key fob simply press ‘skip’ . Bright Instruments...
  • Page 26 At this stage a blank RFID key fob can be placed on the RFID key fob scanner (to the right of the screen) to program the fob for this user. If you are not using a key fob simply press ‘skip’ . Bright Instruments...
  • Page 27 Create New User A6B1D7D818000000 18B1D7C618000000 3403401 3143149 363363 Diana R Michael K Tony A Edit User 169AD9D88D000000 Delete User Exit Exit Delete Return to Home Screen Once you have selected a user, a delete button will appear. Press to delete Bright Instruments...

  • Page 28: Settings Set Clock

    2. Operation Instructions Cont. 2.1 TOUCHSCREEN CONT. 2.1.13 SETTINGS Set Clock SET CLOCK Save changes to Save (ADMIN ONLY) date and time 14 : 38 Time 23 / Date Discard Return to Home Screen Bright Instruments...

  • Page 29: Settings: Event Log

    SP -19.3 CH -22.3 CTRL User IDLE Timeout Date Chamber Temperature User No. Quick Freeze Temperature Specimen Temperature Exit Graph Newer Return to Temperatures and time of Scrolls to Home Screen event log shown in a graph. more records Bright Instruments...
  • Page 30 >> 1125 min period >> 20/08/20 07:32:12 Time Exit Positive Negative Full Scale Reverts to full screen showing positive and Return to Zooms in. Zooms in. negative Home Screen Only shows Only shows temperatures temperatures temperatures above zero below zero Bright Instruments...

  • Page 31: Cabinet Controls

    2. Operation Instructions Cont. 2.2 CABINET CONTROLS 2.2.1 AROUND TOUCHSCREEN Refer to Figure 1. Emergency Stop (High Spec Models Only) RFID Key Fob Scanning: Hover key fob over area Bright Instruments...

  • Page 32: Mains Switch

    On the High spec unit, the handwheel has a locking pin to lock the handwheel position. Beginning End of of travel travel Bright Instruments...

  • Page 33: Foot Switch

    If the Advance/Rewind switch is pressed for more than 1.5 seconds, the specimen travels faster. b. To fully advance/rewind the head press and hold the switch. c. To move small rewind increments, quickly press the switch in the intended direction. Bright Instruments...

  • Page 34: Microtome Controls Solid Knife

    2.3.1 KNIFE GUARD Step 1: Preparation knife out through to the left. a. Install a sharp Bright microtome knife, The knife guard is fitted to the knife block See the table below for a list of recommended setting the appropriate clearance angle for with a retaining wire to ensure it cannot be knife angles.

  • Page 35: Microtome Controls Quick Rel

    Sectioning: A Problem Solving Guide, for b. Lightly tighten the Anti-Roll adjustment a blade is fitted, the guard should be in the further assistance. lock so that the Anti-Roll adjustment knob closed position for operator safety. can still be turned. Bright Instruments...

  • Page 36: Anti-Roll Assembly

    Step 1: Preparation will start to slide under the plate. surfaces using a clean, dry, soft paper towel. a. Install a sharp Bright microtome knife, setting the appropriate clearance angle for e. Tighten the Anti-roll adjustment lock then a. Place the Roll plate against the knife to that particular knife.

  • Page 37: Common Microtome Controls

    0.5 - 30µm are fitted, a conversion factor is to turn anticlockwise will move the knife away needed. from the specimen. When set in the desired position lower the knife block clamp lever to lock in position. Bright Instruments...

  • Page 38: Cutting And Collecting Sections

    Alternatively, if the quick freezer plate has frosted over too much then object holders may be stood on the top of it. Bright Instruments...
  • Page 39 Proceed as follows: your specimen. a. Follow steps 1 to 4 above. b. Watch the embedding compound and suddenly it will be seen to freeze as it turns from transparent to a dense white colour. Bright Instruments...

  • Page 40: Trimming The Specimen

    Keep the knife clear of debris and subsequent very thick section and may frost by brushing upwards with the knife damage the specimen. cleaning brush. Never brush along or down the knife, always brush upwards. Bright Instruments...

  • Page 41: Frozen Sectioning

    • Is the specimen holder tightly clamped? • Is the section thickness setting appropriate? at the right temperature, be patient and wait • Is the orientation lever locked? (Orientating for a while until the temperatures equalise. head microtomes only) • Has the specimen dehydrated? Bright Instruments...

  • Page 42: Specific Problems

    The main area of static Excessive compression of sections: electricity build-up is on the anti-roll plate. When removing frost and debris always • Knife not sharp enough brush up the knife, never down or along. • Knife tilt angle too high Bright Instruments...

  • Page 43: Cutting Temperatures

    Muscle after fixation. Cutting temperatures around (Add 5°C to 10°C for fixed tissue.) Connective Tissue -10ºC to -15ºC are recommended, but some experimenting may be required. Ensure Skin fixative is rinsed off tissue before freezing. Heart Pancreas Uterus Bright Instruments...

  • Page 44: Care & Maintenance

    Use a hair dryer if necessary. e. Place the microtome on the laboratory but is commonly once a month. Note that the microtome requires no other bench and clean it. Refit the rewind knob and routine maintenance. Bright Instruments...

  • Page 45: Microtome Knives

    Cont. 4.4 MICROTOME KNIVES on a conventional knife sharpening machine. If a service visit is required, the cryostat Alternatively, Bright Instrument Co Ltd offer a should be defrosted, decontaminated (refer Great care must be exercised when knife sharpening service. to section 4.8), cleaned thoroughly and left...

  • Page 46: Rest Of World

    4.6 SERVICING AND REPAIRS CONT. 4.6.1 UK • Cabinet Number (see ID plate on rear panel) For customers in the UK, Bright Instrument Co • Microtome Number (see ID plate on rear If the cryostat or any part of it is returned...
  • Page 47 Close the window. e. Leave for one hour. The cryostat is now decontaminated. For further information regarding alternative decontamination procedures please refer to ‘Safe Working and the Prevention of Infection in Clinical Laboratories’ , ISBN 0 11 885446 1. Bright Instruments...

  • Page 48: Cryostat

    Packing dimensions: H1540 x W900 x D1060mm Shipping volume: 1.632m Weights vary according to specification of instrument DO NOT SCALE! DO NOT SCALE! IF IN DOUBT ASK! IF IN DOUBT ASK! 06/09/2019 06/09/2019 1:20 1:20 Bright Instruments DECIMAL: DECIMAL: 0,10 0,10...

  • Page 49: Specifications

    • Requires RFID tag or password to access the touch screen controls. Remaining Feed Indicator Visual display. Dimensions H300 x W300 x D500mm Weight Net weight (Manual): 20kg Gross weight (Manual): 25kg Weights vary according to specification of instrument Bright Instruments...

  • Page 50: Accessories

    Accessories Bright Instruments...

  • Page 51: Product

    OTF6000/LS-002 - 110/115V AC, 50/60Hz Solid blade • Quick freezer - 9 position, running at 10°C below chamber OTF6000/LS-003 for 220V/240V AC, 50/60Hz Quick Release Feather blade OTF6000/LS-004 for 110/115V AC, 50/60Hz Quick Release Feather blade • Minimum chamber temperature: Single compressor -35°C •...

  • Page 52: Equipment Options

    OTF6000 Cryostat Order List 2021 EQUIPMENT OPTIONS OBJECT HOLDERS Code Description Low Spec High Spec Code Description Footswitch for motor drive Leaves hands free Optional Standard 50734-1* Object holder, 22mm diameter, solid. to collect sections. (P/Nbr: 138026). 50734-2 Object holder, 22mm diameter, solid, as 50734-1 but long type.

  • Page 53: Knives & Blades

    53581-1* Bright Cryo-M-Bed 120ml bottle. 57713-1* Bright Cryospray 134 300ml areosol can. 57713 Bright Cryospray 134 300ml areosol can, carton of 12 cans. Instruments Office: Telephone: +44 (0) 808 168 9697 *Included in standard order. **Additional Options. Bright Instruments LTD Web: brightinstruments.com...

  • Page 54: Material Safety Data Sheets

    Material Safety Data Sheets Bright Instruments...

  • Page 55: Cryo-M-Bed 53581

    Goggles should be worn. Instruments OES: Not assigned (long term, 8 hour TWA). Office: Telephone: +44 (0) 808 168 9697 Web: brightinstruments.com Bright Instruments LTD Burnett House, Lakeview Court Email: sales@brightinstruments.com Member: Instruments Ermnie Business Park, Huntingdon Cambridgeshire, PE29 6UA...

  • Page 56: Cryospray 1234Ze

    2. Hazards Identifi cation Classifi cation of the Classifi cation according to Gases under pressure, Liquefi ed gas H280 Contains gas Trade/Type: Bright Cryospray 1234ze substance or mixture: Regulation (EC) No under pressure; may explode if heated. Freezing Agent. 1272/2008/EC (CLP/GHS):...
  • Page 57 Further information In the event of fi re, cool tanks with water spray. Instruments Office: Telephone: +44 (0) 808 168 9697 Web: brightinstruments.com Bright Instruments LTD Burnett House, Lakeview Court Email: sales@brightinstruments.com Member: Instruments Ermnie Business Park, Huntingdon Cambridgeshire, PE29 6UA...
  • Page 58 Eye protection: Goggles. Skin and body protection: Wear suitable protective equipment. Protective footwear. Instruments Office: Telephone: +44 (0) 808 168 9697 Web: brightinstruments.com Bright Instruments LTD Burnett House, Lakeview Court Email: sales@brightinstruments.com Member: Instruments Ermnie Business Park, Huntingdon Cambridgeshire, PE29 6UA...
  • Page 59 MSDS are available for professional users on request. Instruments Office: Telephone: +44 (0) 808 168 9697 Web: brightinstruments.com Bright Instruments LTD Burnett House, Lakeview Court Email: sales@brightinstruments.com Member: Instruments Ermnie Business Park, Huntingdon Cambridgeshire, PE29 6UA...
  • Page 60 Not in compliance with Ems number F-C,S-V published by ERMA New Zealand. inventory. Marine pollutant Instruments Office: Telephone: +44 (0) 808 168 9697 Web: brightinstruments.com Bright Instruments LTD Burnett House, Lakeview Court Email: sales@brightinstruments.com Member: Instruments Ermnie Business Park, Huntingdon Cambridgeshire, PE29 6UA...
  • Page 61 An environmental hazard cannot be excluded in the event of unprofessional handling or disposal. Instruments Office: Telephone: +44 (0) 808 168 9697 Web: brightinstruments.com Bright Instruments LTD Burnett House, Lakeview Court Email: sales@brightinstruments.com Member: Instruments Ermnie Business Park, Huntingdon...

  • Page 62: Low Temperature Oil 57491

    Will not evaporate or dissolve Air: DO NOT allow to enter drainage systems, rivers or waterways. Instruments Office: Telephone: +44 (0) 808 168 9697 Web: brightinstruments.com Bright Instruments LTD Burnett House, Lakeview Court Email: sales@brightinstruments.com Member: Instruments Ermnie Business Park, Huntingdon Cambridgeshire, PE29 6UA...
  • Page 63 Regulatory information: This product is a preparation and is NOT classifi ed according to EEC Guideline. Instruments Office: Telephone: +44 (0) 808 168 9697 Web: brightinstruments.com Bright Instruments LTD Burnett House, Lakeview Court Email: sales@brightinstruments.com Member: Instruments Ermnie Business Park, Huntingdon...

  • Page 64: References & Drawings

    References & Drawings Bright Instruments...
  • Page 65 Figure 1. OTF6000 1. Advance/Rewind Switch 5. Touchscreen 8. Handwheel 2. Automatic Demist Window 6. RFID Key Fob Scanning 9. Waste Liquid Collection Cabinet 3. USB 7. Emergency Stop 10. Foot Switch (High Spec Models Only) (High Spec Models Only) 4.
  • Page 66 12. Knife Block Clamp Locking Lever Solid Knife 3. Anti-Roll Adjustment Lock 8. Manual Advance/Rewind Plug 13. Section Thickness Control 4. Anti-Roll Angle Adjustment Bracket 9. Anti-Roll plate 14. Coarse Advance Control 5. Anti-Roll Angle Adjustment Knob 10. Solid Knife Bright Instruments...

  • Page 67: Assembly

    Anti-Roll plate then tighten to hold in position Move Anti-Roll plate forwards Move Anti-Roll plate backwards Turn Dial Turn Dial DO NOT SCALE! IF IN DOUBT ASK! 10/09/2019 DECIMAL: 0,10 Bright Instruments 1 OF 1 WHOLE NUMBERS: 0,50 APP'D 3RD ANGLE...
  • Page 68 10. Blade Clamping Lever Quick Release Feather 3. Anti-Roll Adjustment Lock 7. Anti-Roll Plate 11. Knife Block Clamp Locking Lever Blade Holder (QRFBH) 4. Blade Clamping Plate 8. Blade Loading Plate 12. Section Thickness Control 13. Coarse Advance Control Bright Instruments...
  • Page 69 SURFACES TO BE CLEAN AND SCRATCH FREE. Blade Holder (QRFBH) Anti-Roll Assembly Tighten to lock Anti-Roll plate in position Move Anti-Roll plate forwards Move Anti-Roll plate backwards Turn Dial Turn Dial Bright Instruments DO NOT SCALE! IF IN DOUBT ASK!

  • Page 70: Decontamination Certificate

    CERTIFICATE Department: Postcode: Customer input Company: Telephone: Any product which is to be returned to Bright Instrument Company Limited or serviced on site, Serial No. Email: must be cleaned and decontaminated in the appropriate manner. This certifi cate, duly completed, Product No.

  • Page 71: Quality Survey Report

    MISCELLANEOUS: Any other aspect the QA Manager. you would like to comment on, e.g. appearance, fi rst impressions etc. Date: Name: Signed. Instruments Office: Bright Instruments LTD Telephone: +44 (0) 808 168 9697 Web: brightinstruments.com Email: sales@brightinstruments.com Member: Burnett House, Lakeview Court Instruments...
  • Page 72 Instruments Instruments Instruments Instruments Burnett House Lakeview Court Ermnie Business Park Huntingdon Cambridgeshire Instruments PE29 6UA +44 (0) 808 168 9697 brightinstruments.com Instruments Member of:...

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