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BRIGHT M3500 MICROTOME Operating, Maintenance And Spare Parts Manual

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Operating, Maintenance and

Spare Parts Manual for the

Bright

M3500 MICROTOME

Serial Number: ...........................

M3500 Issue 2 - Feb 2006
1
Last reviewed: Oct 2010 / Next review Oct 2013

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Summary of Contents for BRIGHT M3500 MICROTOME

  • Page 1: Operating, Maintenance And

    Operating, Maintenance and Spare Parts Manual for the Bright M3500 MICROTOME Serial Number: ……………………… M3500 Issue 2 - Feb 2006 Last reviewed: Oct 2010 / Next review Oct 2013...

  • Page 2: Table Of Contents

    CONTENTS OPERATING, MAINTENANCE AND .............. 1 SPARE PARTS MANUAL FOR THE .............. 1 BRIGHT ......................1 M3500 MICROTOME ..................1 Serial Number: ……………………… ....................1 CONTENTS ................... 2,3 & 4 SAFETY AND GENERAL INFORMATION ............. 6 Consumer Protection .......................... 6 ELECTRICAL ............................
  • Page 3 MOTORISED ADVANCE/REWIND (WHERE FITTED) ......12 2.5.1 General .......................... 12 2.5.2 Motorised Specimen Cassette Rewind ................ 12 2.5.3 Motorised Specimen Cassette Advance ..............12 OTHER FEATURES ................12 2.6.1 Debris Trays ........................12 2.6.2 Visor ..........................13 2.6.3 Hand wheel ........................13 CUTTING AND COLLECTING SECTIONS ..........

  • Page 4: Contents

    DECONTAMINATION PROCEDURES ............28 Microtomes ............................28 Microtome Knives ..........................28 QUALITY SURVEY REPORT................ 29 Annexe: Drawing 212-040 M3500 Microtome Assembly 1 of 3 30, 31 & 32 Part List 212-040-PL 33 & 34 Drawing 51937 Cutting Arm Assembly Part List 51937-PL...
  • Page 5 Section Counter Reset Power Indicator Visor Section Counter Thickness Manual Selector Knob Adv/Rwd Knob Cassette Release Locking Lever Lever Knife Rear Cover Clamps Cutting Handwheel Debris Tray RH Knife Guard Knife Guard RH Advance/Rewind 50230 Standard Height Knob Switch Knife M3500 Issue 2 - Feb 2006 Last reviewed: Oct 2010 / Next review Oct 2013...

  • Page 6: Safety And General Information

    EC Directive to all member states and has been in force with effect from 1 March 1988. The Bright Instrument Company Limited, ever mindful of the need to ensure that their products are not subject to misuse and/or incorrect handling, have made it their aim to communicate any possible dangers to their customers.

  • Page 7: Operation

    DECONTAMINATION CERTIFICATES IMPORTANT If the instrument or any part of it is to be returned to Bright Instrument Company Limited, a decontamination certificate must accompany it, and please note the following: 1. If the instrument or any part of it has been exposed to or been in contact with potential pathogenic or radioactive material, it is essential that it be decontaminated.

  • Page 8: Warranty

    WARRANTY The Warranty relating to the instrument is outlined in our Terms and Conditions of Sale paragraph 6. The Seller‘s products are carefully inspected and submitted to its standard tests. The Seller warrants all its products to be free from defects in workmanship and materials under normal conditions of use and service provided always: that if any of the goods so manufactured is alleged to be defective in workmanship and material and is returned carriage paid, and protected against damage in transit to...

  • Page 9: Introduction

    For instruments with a section counter only, this is operated by 2 AA batteries located on the rear panel at the bottom of the plinth. OPERATING INSTRUCTIONS On receipt of your new Bright instrument, please refer to section 1.1 (Receipt and Unpacking) and section 1.2 (Assembly and Installation). M3500 Issue 2 - Feb 2006...

  • Page 10: Front Control Panel

    As part of its policy of continual improvement, Bright Instrument Company Limited, reserves the right to incorporate changes, or make additions to, without prior notice. There may, therefore, be minor details differences between the information in this manual and your instrument.

  • Page 11: 2.3 Pre-Use Checks

    25º). The correct setting of the knife tilt angle is essential for obtaining good results. With a Bright 50230 knife, set an angle of 15º. With a Bright Lever Release Feather Blade Holder cat no. 57903 the angle should be set to 5º.

  • Page 12: Rewind Control (Manual)

    IMPORTANT: Knife guards must be used whenever a knife or disposable blades are in position. Take care to guard the central portion of the knife whenever sections are not being cut. 2.4.4 Rewind Control (Manual) The rewind control is used to reset the microtome advance system and for trimming specimens.

  • Page 13: Visor

    2.7 CUTTING AND COLLECTING SECTIONS We recommend the following cutting angles when using: leaver release Feather blade - 5º solid knife [Bright 50230] 15º 2.7.1 Set Up fit a sharp knife in position, ensuring knife guards are in central position...

  • Page 14: General Maintenance

    once sections are seen to be cut as required proceed to cut sections NB: For best results the wax cassettes should be cooled to their optimum sectioning temperature Keep the knife clear of debris by brushing upwards with the knife cleaning brush. NEVER brush along the knife, always brush upwards.

  • Page 15: Conventional Knives

    3.2.1 Conventional Knives Conventional microtome knives are usually made from carbon steel and will corrode in moist conditions Bright Instrument Company Limited offer a knife sharpening service, please contact Sales for further information. 3.2.2 Disposable Blades Take particular care to clean the contact surfaces of disposable blade holders. A build up of debris can prevent the blade from seating properly and cuases instability during section cutting.

  • Page 16: Decontamination

    DECONTAMINATION It is the responsibility of the user to ensure that a decontamination procedure is employed which is appropriate to the nature of the work carried out. Hypochlorite (bleach) solutions are corrosive to many metals and should be avoided. Do not expose the debris trays to temperatures in excess of 60ºC. 3.4.1 Disinfectant There are many wide-spectrum biocidal disinfectants on the market that are used in...

  • Page 17: Thick / Thin Sections

    has the microtome reached the end of its travel ? is the section thickness setting appropriate ? THICK / THIN SECTIONS INTERMITTENT FAILURE TO CUT knife/blade not sharp enough knife/blade tilt angle too high/low clamping screws too loose cassette loose on holder SECTIONS CRUMBLING OR NOT FORMING specimen dehydrated knife/blade not sharp enough...

  • Page 18: Sections Fail To Flatten And Pass Down Knife

    SECTIONS FAIL TO FLATTEN AND PASS DOWN KNIFE knife/blade surface has build up debris MISCELLANEOUS SERVICING INSTRUCTIONS Advance Pawl Replacement Remove rear cover by removing the 4 securing screws (rear 2 screws under visor stoppers) and raise and support Drive Arm. Using a thin bladed screwdriver, lever the spring from the hole in the bottom of the pawl.
  • Page 19 Exploded Diagram M3500 Issue 2 - Feb 2006 Last reviewed: Oct 2010 / Next review Oct 2013...
  • Page 20 Internal Construction - Front Internal Construction – Rear M3500 Issue 2 - Feb 2006 Last reviewed: Oct 2010 / Next review Oct 2013...
  • Page 21 9. SPECIFICATIONS M3500 MICROTOME Movement ● Radial, flat cutting with retraction, manual hand wheel operation Cutting Stroke ● 57mm ● Section thickness range 2µm to 30µm in 2µm increments (standard) ● 1µm to 15µm in 1µm increments (Special) ● Total feed range 25mm at 0º...
  • Page 22 Vertical vice clamp 52632 Vertical vice clamp 45° 57713-1 Bright Cryospray 134, 300ml aerosol can 57713 Bright Cryospray 134, 300ml aerosol can, carton of 12 cans 57355 Low temperature grease, 5gms net 57344 Knife cleaning brush M3500 Issue 2 - Feb 2006...
  • Page 23 MATERIAL SAFETY DATA SHEET Name: CRYO-M-BED Part Number: 53581 Product Information Bright Instrument Company Limited Address: St Margaret’s Way, Huntingdon, Cambs, PE29 6EU, England Telephone: 01480 454 528 / 451 499 / 451 980 Emergency: 999 Fax: 01480 456 031 Email: sales@brightinstruments.com...
  • Page 24 01480 454 528 / 451 499 / 451 980 Emergency: 999 Fax: 01480 456 031 Email: sales@brightinstruments.com Trade/Type: Bright Cryospray 134 Aerosol Freezing Agent Container: Aerosol Uses: Rapid Freezing of tissue specimens to –52ºC Data Sheet: Description: Colourless viscous liquid...
  • Page 25 MATERIAL SAFETY DATA SHEET Name: Low temperature Grease Part Number: 57355 Product Information Bright Instrument Company Limited Address: St Margaret’s Way, Huntingdon, Cambs, PE29 6EU, England Telephone: 01480 454 528 / 451 499 / 451 980 Emergency: 999 Email: Fax: 01480 456 031 sales@brightinstruments.com...
  • Page 26 Knives / Blades SAFETY WARNING EXTREMELY SHARP KNIVES / BLADES USE KNIFE / BLADE GUARDS AT ALL TIMES USE CORRECT TOOLS FOR REMOVAL AND INSERTION OF KNIVES / BLADES DO NOT LEAVE KNIVES / BLADES LAYING AROUND PLACE KNIVES / BLADES NOT IN USE, INTO BOX / WALLET PROVIDED M3500 Issue 2 - Feb 2006 Last reviewed: Oct 2010 / Next review Oct 2013...
  • Page 27 HEALTH AND SAFETY AT WORK ACT DECONTAMINATION CERTIFICATE Any product which is to be returned to Bright Instrument Company Limited or serviced on site, must be cleaned and decontaminated in the appropriate manner. This certificate, duly completed, must be either sent in advance (fixed to the outer packing containing the product), or handed to the service engineer.
  • Page 28 DECONTAMINATION PROCEDURES Cryostats & Ultra Low Temperature Freezing Units If decontamination is required carry out the standard procedures as practised in your laboratory. It is the responsibility of the customer to use a decontamination procedure appropriate to his/her work. The following decontamination method is as recommended in the ‘Code of Practise for the Prevention of Infection in Clinical Laboratories and Post-mortem Rooms’, ISBN 0 11 320464 7.
  • Page 29 QA023/ISSUE 4/AUG99(F018) QUALITY SURVEY REPORT Our watchword is QUALITY. In our continuing endeavour to improve the quality and performance of our processes and products, we would welcome any initial comments on the following aspects of our service and products. As you have only just received the product we do not feel that you could asses the actual workings of the instrument accurately, so we will follow up in approximately six months with a Customer Feedback –...
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  • Page 33 52119 M3 X 12 SKT CAP HD ST/ST 55120 ISSUE CHECKED APPROVED AUTHORISED DATE CN 2371 07/03/2006 Page 1 of 2 BRIGHT INSTRUMENT Co Ltd - COPYRIGHT PROTECTED - ALL RIGHTS RESERVED M3500 Issue 2 - Feb 2006 Page 33...

  • Page 34: M3500 Issue 2 - Feb 2006

    ** M3500 KNIFE BLOCK RETRACT ASSY 212-054 ** M3500, INPUT DRIVESHAFT 212-061 (HANDWHEEL) ASSEMBLY ISSUE CHECKED APPROVED AUTHORISED DATE CN 2371 07/03/2006 Page 2 of 2 BRIGHT INSTRUMENT Co Ltd - COPYRIGHT PROTECTED - ALL RIGHTS RESERVED M3500 Issue 2 - Feb 2006 Page 34...
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  • Page 36 M3 PLAIN WASHER ST/ST MAX OD 7mm 55610 M5 X 5 GRUB SCREW BZP 50958 ISSUE CHECKED APPROVED AUTHORISED DATE CN 2371 06/03/2006 Page 1 of 1 BRIGHT INSTRUMENT Co Ltd - COPYRIGHT PROTECTED - ALL RIGHTS RESERVED M3500 Issue 2 - Feb 2006 Page 36...
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  • Page 38 55033 M4 PLAIN WASHER ST/ST 55616 M4 NYLON WASHER 55633 ISSUE CHECKED APPROVED AUTHORISED DATE CN2371 17/02/2006 Page 1 of 1 BRIGHT INSTRUMENT Co Ltd - COPYRIGHT PROTECTED - ALL RIGHTS RESERVED M3500 Issue 2 - Feb 2006 Page 38...
  • Page 40 M4 x 4 SOCKET GRUB SCREW BZP 55000 M6 X 6 GRUB SCREW BZP 55008 ISSUE CHECKED APPROVED AUTHORISED DATE CN2371 07/03/2006 Page 1 of 1 BRIGHT INSTRUMENT Co Ltd - COPYRIGHT PROTECTED - ALL RIGHTS RESERVED M3500 Issue 2 - Feb 2006 Page 40...
  • Page 42 M3 X 5 GRUB SCREW BZP 50947 1/16 X 1/4 SPRINGPIN ST/ST 55904 ISSUE CHECKED APPROVED AUTHORISED DATE CN2371 07/03/2006 Page 1 of 1 BRIGHT INSTRUMENT Co Ltd - COPYRIGHT PROTECTED - ALL RIGHTS RESERVED M3500 Issue 2 - Feb 2006 Page 42...
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  • Page 44 M3 x 8 SKT CAP HD ST/ST 55101 M3 FULL NUT ST/ST 50942 ISSUE CHECKED APPROVED AUTHORISED DATE CN2371 20/02/2006 Page 1 of 1 BRIGHT INSTRUMENT Co Ltd - COPYRIGHT PROTECTED - ALL RIGHTS RESERVED m3500 Issue 2 - Feb 2006 Page 44...
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  • Page 46 M3 X 6 CSK SLTD HEAD ST/ST 51550 M3 X 6 SOCKET CAP HEAD ST/ST 50952 ISSUE CHECKED APPROVED AUTHORISED DATE CN2378 08/05/2006 Page 1 of 2 BRIGHT INSTRUMENT Co Ltd - COPYRIGHT PROTECTED - ALL RIGHTS RESERVED M3500 Issue 2 - Feb 2006 Page 46...
  • Page 47 212-427 5040 KNIFE BLOCK POINTER 50526 PLUNGER BALL 6MM 51341 ISSUE CHECKED APPROVED AUTHORISED DATE CN2378 08/05/2006 Page 2 of 2 BRIGHT INSTRUMENT Co Ltd - COPYRIGHT PROTECTED - ALL RIGHTS RESERVED M3500 Issue 2 - Feb 2006 Page 47...
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  • Page 49 M4 x 4 SOCKET GRUB SCREW BZP 55000 M4 X 6 GRUB SCREW BZP 55001 ISSUE CHECKED APPROVED AUTHORISED DATE CN2371 20/02/2006 Page 1 of 1 BRIGHT INSTRUMENT Co Ltd - COPYRIGHT PROTECTED - ALL RIGHTS RESERVED M3500 issue 2 - Feb 2006 Page 49...

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