BRIGHT OTF 5000 Instruction Manual
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BRIGHT OTF 5000 Instruction Manual

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Serial No:
St Margarets Way Stukeley Meadows Industrial Estate Huntingdon Cambridgeshire England PE29 6EU
Tel: +44 (0) 1480 454528/451980/451499 . Fax: +44 (0) 1480 456031
Email: sales@brightinstruments.com . Website: www.brightinstruments.com
INSTRUCTION MANUAL
OTF 5000 All Models
Model shown HS
BS EN ISO 9001:2008
Certificate No. Q 05456

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  • Page 1 INSTRUCTION MANUAL OTF 5000 All Models Model shown HS Serial No: St Margarets Way Stukeley Meadows Industrial Estate Huntingdon Cambridgeshire England PE29 6EU Tel: +44 (0) 1480 454528/451980/451499 . Fax: +44 (0) 1480 456031 Email: sales@brightinstruments.com . Website: www.brightinstruments.com BS EN ISO 9001:2008...

  • Page 3: Table Of Contents

    OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 CONTENTS SAFETY INFORMATION CONSUMER PROTECTION ELECTRICAL MICROTOMES KNIVES/BLADES OPERATION LOW TEMPERATURES ACCESSORIES PRODUCT SAFETY SUGGESTIONS DECONTAMINATION CERTIFICATES WARNING WARRANTY 1.1 RECEIPT AND UNPACKING 1.1.1 RECEIPT 1.1.2 UNPACKING 1.2 ASSEMBLY AND INSTALLATION 1.2.1 FITTING THE HANDWHEEL 1.2.2...
  • Page 4 OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 2.1.3 DEFROST SWITCH (Located on the side panel) 2.1.4 DEFROST CLOCK (Located on side panel) 2.1.5 CHAMBER TEMPERATURE 2.1.6 /D - CRYOMATIC SPECIMEN TEMPERATURE CONTROL (where applicable) 2.1.7 QUICK FREEZER PLATE 2.1.8 /QF - SPECIMEN QUICK FREEZER (where applicable) 2.1.9...
  • Page 5 OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 3.7.2 FIXED TISSUE 4.1 DAILY CARE 4.2 AUTOMATIC DEFROST CYCLE 4.3 TOTAL DEFROSTING 4.4 MICROTOME KNIVES 4.5 OPERATING IN EXTREME CONDITIONS 4.6 SERVICING AND REPAIRS 4.6.1 4.6.2 REST OF WORLD 4.7 SIMPLE SERVICING/REPAIR PROCEDURES 4.7.1...
  • Page 6 OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 PRODUCT INFORMATION SPECIAL FEATURES: Configurations Basic Specification LS Specification HS Specification Appendices 5040 MICROTOME DRAWINGS (relevant to Model) 5040 MICROTOME PART LIST (relevant to Model) ELECTRICAL CIRCUIT DIAGRAMS (relevant to Model) Material Safety Sheet: CRYOSPRAY 57713...

  • Page 7: Safety Information

    EC Directive to all member states and has been in force with effect from 1st March 1988. Bright Instrument Co Ltd, ever mindful of the need to ensure that their products are not subject to misuse and/or incorrect handling, have made it their aim to communicate any possible dangers to their customers.

  • Page 8: Low Temperatures

    DECONTAMINATION CERTIFICATES IMPORTANT If the instrument or any part of it is to be returned to Bright Instrument Co Ltd, please note the following: 1 If the instrument or any part of it has been exposed to or been in contact with potential pathogenic or radioactive material, it is essential that it be decontaminated.

  • Page 9: Warranty

    1.1.1 RECEIPT Immediately upon receipt of the instrument, make a careful examination for evidence of damage encountered in transit. If any damage is found or suspected, notify both the carrier and Bright Instrument Co Ltd immediately. 1.1.2 UNPACKING All packing must be carefully removed and parts checked against the enclosed packing list.

  • Page 10: Assembly And Installation

    OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 ASSEMBLY AND INSTALLATION 1.2.1 FITTING THE HANDWHEEL Push the handwheel over the shaft on the right side of the cabinet. Ensure the pin on the shaft engages the slot in the centre of the handwheel. Press firmly home, then fit and tighten the centre screw.

  • Page 11: Frost Elimination Cover - Fig

    OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 1.2.4 FROST ELIMINATION COVER – FIG 3 Frost Elimination Cover Place on the quick freezer plate 1.2.5 INSTALLATION OF THE MICROTOME (and REMOVAL) WARNING - The microtome weighs approximately 15kg. Ensure that adequate precautions are taken to prevent injury, i.e.

  • Page 12: Insulated Night Plug

    OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 7 For /MAR - Motorised Advance Rewind (where applicable): When fitting the microtome, the electrical socket and plug must be connected and screwed together. Before removing the microtome from the cryostat, the electrical socket and plug must be unscrewed and disconnected.

  • Page 13: Positioning

    OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 1.2.11 POSITIONING Fit the two spacer brackets to the rear of the cabinet and ensure that nothing will block the vents on this rear panel. The instrument should be positioned on a level floor so that an unrestricted air flow through the cooling vents is obtained.

  • Page 14: Operating Instructions

    FIG 1 SWITCHING ON On receipt of your new Bright instrument, please refer to the Receipt and Unpacking section and the Assembly and Installation section of this Handbook As part of its policy of continual improvement, Bright Instrument Company Limited reserves the right to incorporate changes, or make additions to, without prior notice.

  • Page 15: Cabinet Controls

    OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 CABINET CONTROLS Temperature Alarm Fig 5 FIG 6 2.1.1 MAINS SWITCH (Located on side panel) This switch is used to connect the power supply to the cryostat. Operate this switch to switch the instrument on and off.

  • Page 16: Chamber Temperature

    If Up or Down have not been pressed (i.e. no new parameters have been entered), simply leave for 20 seconds. Display will revert to normal. b If new parameters have been selected contact Bright Instrument Co Ltd or your local representative for advice.

  • Page 17: D - Cryomatic Specimen Temperature Control (Where Applicable)

    OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 2.1.6 /D - CRYOMATIC SPECIMEN TEMPERATURE CONTROL (where applicable) FIG 8 To set the specimen temperature, press and release the Set button and then press the Up or Down buttons until the desired temperature is set. After 5 seconds the actual temperature will show.

  • Page 18: Ec Or /Lt Or /Lowec - Dual Refrigeration System (Where Applicable)

    OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 3 Place the /QF cover over the /QF, this will reduce the rate that frost forms. 4 Switch the /QF on at least 30 minutes before it is required for use. Please note that in certain conditions the /QF compressor will not operate immediately.

  • Page 19: Motorised Advance/Rewind (Where Applicable)

    OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 2.1.10 MOTORISED ADVANCE/REWIND (where applicable) FIG 12 Warning: Do not operate the rewind motor when the cutting arm is in motion. Only operate the rewind motor with the cutting arm at the rest position. The advance motor may be operated when the cutting arm is in motion.

  • Page 20: Mr Or /Mr(S) - Motor Drive (Where Applicable)

    OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 2.1.12 /MR or /MR(S) - MOTOR DRIVE (where applicable) To operate the motor drive: 1 Rotate the handwheel while pushing the small connecting rod knob. The rod will engage through to the motor drive, and the handwheel is now locked into the motor drive.

  • Page 21: U - Ultra Violet Light Decontamination Unit (Where Applicable)

    OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 2.1.15 /U - ULTRA VIOLET LIGHT DECONTAMINATION UNIT (where applicable) Following is the recommended use of the /U optional feature. The information given is, to the best of the Company’s knowledge and belief, accurate and reliable. However, no warranty, guarantee or representation is made to its accuracy, reliability or completeness.

  • Page 22: Microtome Controls - Standard Knife Block

    OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 MICROTOME CONTROLS - STANDARD KNIFE BLOCK Refer FIG 16 Section thickness control Knife guard height adjustment screw Knife holder block Knife guard release knob Knife block clamp locking lever Knife guard slider knobs Knife carriage feed control...

  • Page 23: Knife Holder

    OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 2.2.2 KNIFE HOLDER To ensure optimum sectioning performance the knife must be kept clean from grease and dirt. To clean the knife, carefully apply a small amount of methylated spirits or ethanol to the surface of the knife using a clean, dry, soft paper towel.

  • Page 24: Anti-Roll Assembly

    Step 1 – Preparation 1 Install a sharp Bright microtome knife (6), setting the appropriate clearance angle for that particular knife. 2 Clamp a frozen specimen (or embedding compound alone) into the microtome (refer to section 3.2)
  • Page 25 OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 1 Slacken the anti-roll T piece clamp screw (16) and slide the angle adjustment shaft backwards and forwards until approximately 2/3 of the anti-roll plate stem is below the anti- roll T piece clamp screw. Then tighten the anti-roll piece clamp screw.

  • Page 26: Microtome Controls - Quick Release Feather Blade Holder

    OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 MICROTOME CONTROLS - Quick Release Feather Blade Holder FIG 17 Knife Block Clamp Lever Stop Pin Blade Angle Locking Lever Anti-Roll Plate Adjuster Blade Angle Indicator Stem Clamp Knob Wire Knife Guard Blade Blade Loading Plate...

  • Page 27: Blade Guards

    OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 2.3.1 BLADE GUARDS Refer to Figure 17 A pair of fixed blade guards (6) is fitted. These must never be removed under any circumstances. A wire blade guard (4) is also fitted. When access to the blade is required, the wire blade guard can be opened. Turn the wire blade guard knob (15) anticlockwise to open and turn the wire blade guard knob clockwise to close.
  • Page 28 OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 Clamp a frozen specimen (or embedding compound alone) into the microtome (refer to section 3.2). Release the knife block clamp lever (1) and turn the coarse advance control (18) clockwise to bring the blade towards the specimen.

  • Page 29: Common Microtome Controls

    OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 Do not drag the anti-roll plate downwards across the edge of the blade as this will result in possible damage to both the anti-roll plate and the blade. Your anti-roll plate may vary from the design shown, but the set-up procedure remains the same.

  • Page 30: Specimen Holder

    OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 2.4.3.1 /LHS - LEFT HAND SIDE ADVANCE/REWIND CONTROL (where applicable) LHS Advance/Rewind control The operation is exactly the same as for the Advance/Rewind control on the top of the microtome. Please note that this option is not available with Motorised Advance/Rewind 2.4.4 SPECIMEN HOLDER...

  • Page 31: Cutting & Collecting Sections

    OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 Use your left hand to press down on the top of the feed screw (where the rewind knob fits on) and pull towards you. Use upwards counter pressure on the hinge segment with your right hand at the same time.

  • Page 32: Bright Clini-Rf

    3.1.5 BRIGHT CLINI-RF The Bright Clini-RF is a small, free standing, top opening, ultra low temperature freezer. Its function is to rapidly freeze tissue specimens ready for cryotomy or for storage. Inside the Clini-RF are two cylindrical chambers, the upper chamber is held at minus 40ºC and the lower at...

  • Page 33: Fitting The Specimen

    OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 FITTING THE SPECIMEN For setting up tissue, proceed as follows: 1 Remove the frost elimination cover from the quick freezer plate. 2 Make sure that the object holder is above 0ºC, if it is at room temperature that is fine and it just takes a little longer.

  • Page 34: Trimming The Specimen

    OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 TRIMMING THE SPECIMEN 1. Fit a sharp knife in position. Allow the knife to equilibrate to chamber temperature. 2. Lock the handwheel with the specimen holder in the top position. FIG 24 3. Fit the specimen into the microtome.

  • Page 35: Frozen Sectioning - A Problem Solving Guide

    OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 there is not an accumulation of rime present on the underside and leading edge of the anti-roll plate. Care must be taken when using Cryospray 134. The risk of creating an aerosol of potentially infectious tissue particles must be borne in mind.

  • Page 36: Cutting Temperatures

    OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 • freezing technique too slow • cutting temperatures too warm/cold • specimen not equilibrated to chamber temperature • specimen dehydrated • knife not sharp enough • cutting motion too fast or uneven • knife tilt angle too high/low Excessive compression of sections: •...

  • Page 37: Fixed Tissue

    OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 3.7.2 FIXED TISSUE Differences between tissues are much reduced after fixation. Cutting temperatures around - 10ºC to -15ºC are recommended, but some experimenting may be required. Ensure fixative is rinsed off tissue before freezing.

  • Page 38: Operating In Extreme Conditions

    Should no decontamination certificate be received, or the cryostat or any part of it be received in a condition that Bright Instrument Co Ltd consider to be a potential biological hazard, the cryostat or part will be retuned, unrepaired, at the expense of the customer.

  • Page 39: Rest Of World

    User servicing is restricted to changing of fuses and fluorescent light tubes. All other servicing must be carried out by a qualified engineer. Any replacement parts must be ordered from Bright Instrument Co Ltd - no liability can be accepted if non-Bright supplied parts are installed.

  • Page 40: Accessories

    50744 50721 50741 50743 50727 Standard tubular holder, 22mm dia. Fits Bright 5030 microtome only. 50734-1 Solid object holder, 22mm dia. Suitable for most applications 50734-2 Solid object holder 22mm long type, gives greater clearance between specimen clamp and knife holder. Ideal for when multi-position glass slides are used.
  • Page 41 OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 Quick-release type 50203 50690 50204 50203 Quick release object holder. Fits into a standard vice clamp and takes 50690 metal discs 50222-1 Quick release object holder. Complete with 50 off 50690 metal discs 50690 Metal object holder discs, 22mm dia.

  • Page 42: Knives

    OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 KNIVES The following knives are available to fit the 5030 and 5040 microtomes: 50230 Standard knife supplied in box, C profile, 189 x 27 x 10mm, 22º angle, tapped to accept holder for Shandon knife sharpener, safety cut outs at each end.

  • Page 43: Lever Release Feather Blade Holder

    OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 LEVER RELEASE FEATHER BLADE HOLDER 5.3.1.1 Cat. No. 57903 Description The Lever Release Feather Blade Holder has been designed to fit standard knife holders on most microtomes. It accepts low profile S35 blades or similar. It’s shape is similar to a solid knife and has stainless steel pressure pads (not shown) that act as cushions to spread the pressure of the fixing screws as well as blade guides to make it easier to change and insert a new blade.

  • Page 44: Easi-Set, Anti-Roll Plates

    OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 50140 shown in-situ on a 5040 microtome. 50140 Easi-Set, ANTI-ROLL PLATES These anti-roll plates are of unique design having “side wings” that facilitate extremely easy adjustment and give a wide leeway when positioning against the edge of the blade or knife.

  • Page 45: Cryospray

    SPARE PARTS Parts can be obtained through your local Bright representative, or from Bright Instrument Co Ltd. When ordering parts, please provide the following details: Model type and serial number of your instrument...

  • Page 46: Accessories And Consumables

    Adaptor, to accept Ames cryostat, saddle type object holders. 52658 Vertical vice clamp. 57713-1 Bright Cryospray 134, 300ml aerosol can. 57713 Bright Cryospray 134, 300ml aerosol can, carton of 12 cans. 54791 Low temperature oil, 200ml bottle. 54791-1 Low temperature oil, 4.54 litre drum. 57344 Knife cleaning brush.
  • Page 47 PRODUCT INFORMATION Introduction Based on the long-established and reliable OTF/AS cryostat, the new OTF5000 brings Bright cryostats completely up to date. New styling with improved user ergonomics, the latest blade systems in the ever-reliable and powerful 5040 microtome, a huge choice of options plus money-saving package deals make these cryostats absolutely unique.
  • Page 48 OTF 5000/HS/LS-001/2/3/4 Iss 4 June 2012 Retraction on return stroke Approx. 50µm Section range 0.5 to 30µm in 0.5µm increments Maximum head advance 5.6mm Maximum knife block adjustment 44mm (Coarse control) Knife angle adjustment 25º Window surround Heated Window demist system...

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