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Tro uble shooting
12
Troubleshooting
Gel or cellophane cracks
All gels
•
Handle the gel and cellophane with care. Fully hydrate the cellophane with
distilled water just before use and avoid stretching it during assembly.
•
If the cellophane is drying too quickly, instead of hydrating it in distilled
water, soak it in the same glycerol solution used for the gel.
•
Always remove all air bubbles before drying.
•
Lower the heat setting.
•
Load frame assemblies into the dryer from the top down. Avoid dripping
onto a partially dried gel.
Gradient gels or gels
•
Dehydrate the gel in 35% ethanol and 2% glycerol before drying. If gels still
crack, increase the percentage of glycerol up to 5%.
Note: With a higher percentage of glycerol, a longer drying period is
required. The gels may also be flexible and feel tacky when dry.
Fluor-treated gel
•
Use cellophane instead of mylar.
•
Use a thinner gel ( 0.75 mm
Gel dries too slowly or not at all
Gel dries unevenly
•
Rotate each frame 180 ° (front-to-back) every half hour.
Check the gel or stack
•
Reduce the amount of glycerol to 0.5 to 2%
•
Check that you are not using two sheets of Mylar. At least one sheet must
be cellophane.
Check the heater
•
Make sure the indicator lamp lights at the low hear or high hear settings. If it
doesn't light or the heater does not heat, the safety switch may be tripped.
Check that the front and back of the dryer are unobstructed and wait a few
minutes for the dryer to cool, then resume heating.
Gel turns cloudy or white
Gels
mm thick with acrylamide
•
Reduce the percentage of ethanol to 30%.
•
Do not soak the gel for more than 2 hours in the ethanol/glycerol
dehydration solution.
•
After Coomasie Blue staining, be sure to use final destain of 7% acetic acid
and 5% methanol.
Dryer does not function
•
Check that the power switch is on.
•
Check that the power receptacle is properly grounded and in working
condition.
•
Check the fuses and replace, if necessary
•
If the dryer overheats, make sure air can flow freely by checking that both
the front and back are not obstructed. If the dryer overheats more than
once in the same day, call the GE Healthcare Technical Service
Department.
mm thick with acrylamide
T
Hoefer SE 1200 Easy Breez e Air Gel Dryer
T
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