Related Manuals for Leica EM KMR2
Summary of Contents for Leica EM KMR2
- Page 1 Leica EM KMR2 Leica EM KMR2 Leica EM KMR2 Leica EM KMR2 Leica EM KMR2 Operating Manual Operating Manual Operating Manual Operating Manual Operating Manual Order Number 177032 Leica EM KMR2 - GA-E-06/03...
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Page 3: Table Of Contents
2.4 Length of useful edge 3. Unpacking and installation 3.1 Unpacking 3.2 Packing list 3.3 Installation 4. Description of the LEICA EM KMR2 5. Knifemaking 5.1 Hints 5.2 Making squares 5.3 Making knives from a square 5.4 Evaluation of the knife edge 6. -
Page 5: Safety Instructions
1. Safety instructions Prior to the operation of the Leica EM KMR2 read the instruction manual carefully. Always use safety goggles during operation to avoid eye injury. Clean the instrument regularly with a brush to remove glass splinters. Store broken knives safely in the knife boxes provided. - Page 6 Fig.1 Fig.2 Breaking pins Fig.3 Counterpiece/ Knife shoulder Fig.3a Knife edge Knife edge Counterpiece/ Knife shoulder...
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Page 7: Principles Of Knifemaking
Producing good glass knives routinely depends on a supply of reproducible squares, an accurately positioned score and controlled pressure precisely applied to make the break. The LEICA EM KMR2 gives the user a choice of two different scores, each one factory set to produce the optimum break. - Page 8 Fig.4 Scoring angle Real knife angle Fig.5 Stress line...
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Page 9: The Real Knife Angle
When a glass knife edge is examined under darkfield illumination using a stereo microscope (or on LEICA Ultramicrotomes using the back light, chapter 5.4), it can be seen that the central part is most useful for ultrathin sectioning. The right side of the edge has visible marks (saw teeth) which reduce the quality of the knife, and the left corner is also unsuitable for sectioning because of the stress line (Fig.5). - Page 10 Fig.6 Fig.7 Fig.8 Side view Fig.8...
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Page 11: Unpacking And Installation
LEICA KMR2 3.3 Installation Place the Leica EM KMR2 on a sturdy laboratory bench or a table where it will not be affected by movement from other laboratory equipment, such as a centrifuge. The Leica EM KMR2 is completely assembled in the factory, so the instrument is ready to... - Page 12 Fig.9...
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Page 13: Description Of The Leica Em Kmr2
4. Description of the LEICA EM KMR2 The scoring and breaking mechanism for knife making are assembled on a heavy, vibration-absorbing base. The parts are shown in Fig.9: 1. Clamping lever: is used to lower and raise the scoring head and also to clamp the scoring head to the clamping head (No.2). - Page 14 Fig.10 Moveable stop at position 1. Fig.11 Fig.11a...
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Page 15: Knifemaking
5. Knifemaking 5.1 Hints For the best results from your Leica EM KMR 2 it is recommended that glass strips supplied by Leica are always used. Leica glass strips for ultramicrotomy are produced from specially selected glass, the thickness and quality of which is precisely controlled. - Page 16 Fig.12 Fig.13 Fig.14 Fig.15 Fig.16 Fig.17 Moveable stop at position 2.
- Page 17 Set the scoring mark selector (Fig.9 No.6) to its long mark. Score the glass by fast and evenly pulling the scoring shaft as shown in Fig.12. Rotate the breaking lever gently and slowly clockwise to initiate the break as shown in Fig.13. After the break, raise the scoring head completely by means of the clamping lever.
- Page 18 Fig.21 Freshly broken side! Freshly broken side! Fig.22 Notch of the rear stop. Notch of the front stop. Fig.23...
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Page 19: Making Knives From A Square
Note: As the left end of the left hand 200 mm glass strip is machine broken by the glass manufacturers and therefore geometrically not perfect do not use this square for breaking knives (Fig.19). Also, the outer square of the right hand 200 mm glass strip is not used to make knives (Fig.20) for the same reason. - Page 20 Fig.24 Fig.25 Fig.26 Fig.27...
- Page 21 After breaking raise the scoring head completely by means of the clamping lever. Support head with finger below the scoring shaft to prevent it droping onto the glass. Push back the scoring shaft and bring the breaking lever to its starting point by rotating anticlockwise.
- Page 22 Fig.28 Fig.29 Fig.30 Fig.31 Left hand Right hand knife knife Fig.32...
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Page 23: Evaluation Of The Knife Edge
5.4 Evaluation of the knife edge After making a pair of knives, evaluation of the quality can be carried out with the Ultramicrotome (Leica Ultracut UCT / R / S). Using the backlight illumination and setting the clearance angle to maximum a fine white line can be seen (Fig.28). -
Page 24: Knife Shoulder Fine Adjustment
As mentioned earlier in this manual the sharpest knife edge is found opposite a very small knife shoulder. Fine adjustments on the Leica EM KMR2 allow you to adjust the size of the knife shoulder for either resin or cryo sectioning. - Page 25 ‘B’ to 12 o´clock using the 2 mm Allen Key provided. This is then your starting point . If the Leica EM KMR2 is adjusted for resin sectioning and you wish to have smaller knife shoulders for cryosectioning then your starting point is the factory setting, To start with, follow the scoring and breaking procedure as described in chapter 5.2 and 5.3.
- Page 26 Fig.34 Fig.35 Sliding surfaces Fig.36...
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Page 27: Care And Maintainance
7. Care and maintenance The Leica EM KMR2 should always be kept clean.The breaking pins must be free of any glass particles. Use a brush or ‘Dust off’ for cleaning. The sliding surface of the scoring and clamping head (Fig. 34 and Fig.35) must also be kept clean and dry . - Page 28 Fig.39 Fig.40 Fig.41...
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Page 29: Adjusting The Scoring Pressure
7.2 Adjusting the scoring pressure Raise the scoring head clompletely as shown in Fig.36 and place it on the table upside down. By turning the screw (Fig.39) with the 2 mm Allen Key (provided) clockwise the pressure can be increased, counter clockwise the pressure can be decreased. After turning the screw in the required direction (a quater to a half turn) replace the scoring head and make a test score on a piece of glass. - Page 30 Fig.42 Hotplate Wax bath Fig.43 Fig.44 Fig.45...
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Page 31: Mounting Troughs ('Trufs') Onto The Knife
Fitting a Truf is simple if the Leica EM MP is used (Fig.42). Wax is melted in the bath of the Leica EM MP, glass knives are lightly warmed on the hotplate with the knife edge overhanging the edge of the hotplate as shown in Fig.43. -
Page 32: Accessories And Consumables
89 50 32 Dust cover 70 52 25 Knife boxes (3 pcs.) 70 52 27 Scoring wheels for Knifemaker1 (3 pcs.) 70 66 99 Scoring wheel cartridge for Leica EM KMR2, KMR and Knifemaker II Recommended accessories 70 54 01 LEICA EM MP 115/230VAC complete with metal spatula and 500g dental wax. -
Page 33: Technical Specifications
Special glass for Ultramicrotomy, Cryo-ultramicrotomy and Histology Length 400 mm Width 25 mm Thickness 6.4 /8 /10 mm Scoring Wheel: Tungsten carbide in cartridge Leica EM KMR 2 Dimensions and Weight: Length: 400 mm Depth: 280 mm Height: 280 mm... - Page 34 Leica Mikrosysteme GmbH Tel.: +43 1 48899 Hernalser Hauptstrasse 219 Fax.: +43 1 48899-350 A-1170 Wien, Austria www.em-preparation.com...