Related Manuals for TSE Calorimetry PhenoMaster
Summary of Contents for TSE Calorimetry PhenoMaster
- Page 1 Sophisticated Life Science Research Instrumentation PhenoMaster Hardware Operating Instructions Calorimetry © www.TSE-Systems.com Info@TSE-Systems.com...
- Page 2 These operating instructions, including all parts thereof, are protected by copyright. Any infringement of the copyright laws without the written agreement of the TSE Systems GmbH is illegal and subject to due penalty at law. This applies in particular to their reproduction, processing, translation, microfilming as well as to their storage and processing in electronic systems.
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Page 3: Table Of Contents
PC Requirements ......................9 Installation Drivers, Interface, Software ................. 10 5.1. USB Interface ........................ 10 5.1.1. Installing *.inf file (TSE USB Adapter*) ................10 5.2. WireOne Driver......................12 Connecting Devices - General ..................16 Cage Rack ........................16 System Software ......................16 Performing a Measurement ................... - Page 4 Connections Protection Air Filter ................... 62 11.9. Air Drying Unit ....................... 64 11.9.1.1. 1x Air Drying Unit ......................65 11.9.1.2. 4x Air Drying Unit ......................66 11.9.2. Connections Air Drying Unit................... 67 Appendix A. Example configurations B. Example electrical requirements info@TSE-Systems.com www.TSE-Systems.com...
- Page 5 Fig. 33 Pressure control valve ......................61 Fig. 34 Connections protection air filter .................... 63 Fig. 35 Flow direction ........................63 Fig. 36 1x Air Drying Unit w/ separating bottle ................. 65 Fig. 37 4x Air Drying Unit ......................... 66 info@TSE-Systems.com www.TSE-Systems.com...
- Page 6 PhenoMaster | Hardware Operating Instructions info@TSE-Systems.com www.TSE-Systems.com...
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Page 7: Introduction
These operating instructions describe devices and features of the PhenoMaster Phenotyping Research Platform of TSE Systems. Some devices and features may not be available on your system. For your individual system configuration, please refer to your order confirmation. If you have questions or need assistance, please contact Service. -
Page 8: Instructions For Safe Operation
• After you have discarded condensed water of separating bottle, carefully reconnect bottle to tubing. • Select "Status/Calo" and check for proper "SampleFlow” on "Calo Current Values" dialog (see Software Operating Instructions – Calorimetry, Section "Status Menu, Calo"). info@TSE-Systems.com www.TSE-Systems.com... -
Page 9: Operating Principle
CaloSys Modules Operating Principle See Hardware Operating Instructions - Core Modules, Section "Operating Principle". PC Requirements See Hardware Operating Instructions - Core Modules, Section "PC Requirements". info@TSE-Systems.com www.TSE-Systems.com... -
Page 10: Installation Drivers, Interface, Software
USB interface If your Calibration Control Unit has an USB connection to the PC, no PCI interface is required. 5.1.1. Installing *.inf file (TSE USB Adapter*) It is assumed that… • …the Calibration Control Unit has already been connected to the PC. - Page 11 CaloSys Modules • Press "Install". • Confirmation dialog • Check "Device Manager" dialog. The list item with the attention symbol should have disappeared and under "Universal Serial Bus controllers", a new entry "USB Bulk driver" should have been created. info@TSE-Systems.com www.TSE-Systems.com...
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Page 12: Wireone Driver
PhenoMaster | Hardware Operating Instructions 5.2. WireOne Driver Follow default installation routine. • Double-click install_1_wire_drivers*.msi • Press "Next" • Check "I accept the terms in the License Agreement" and press "Next". info@TSE-Systems.com www.TSE-Systems.com... - Page 13 CaloSys Modules • Confirm default installation path by pressing "Next". info@TSE-Systems.com www.TSE-Systems.com...
- Page 14 PhenoMaster | Hardware Operating Instructions • Press "Install". • Press "Install". info@TSE-Systems.com www.TSE-Systems.com...
- Page 15 CaloSys Modules • Press "Finish". info@TSE-Systems.com www.TSE-Systems.com...
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Page 16: Connecting Devices - General
Cage Rack See Hardware Operating Instructions - Core Modules, Section "Cage Rack". System Software See Hardware Operating Instructions - Core Modules, Section "System Software". Performing a Measurement See Hardware Operating Instructions - Core Modules, Section "Performing a Measurement". info@TSE-Systems.com www.TSE-Systems.com... -
Page 17: Maintenance And Cleaning
In order to clean and decontaminate calorimetry cage lids, it is necessary to dismantle them completely. WARNING Eye Injuries When the quick-action coupling plug is loosened, it may pops out and injure your eyes. Hold the quick-action coupling plug tightly when loosening it and do NOT bend down over it. info@TSE-Systems.com www.TSE-Systems.com... - Page 18 PhenoMaster | Hardware Operating Instructions info@TSE-Systems.com www.TSE-Systems.com...
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Page 19: Fig. 2 Calorimetry Cage Lid
Lens head screw M3x18 cross recess 1.4031 steel Lens head screw M3x10 cross recess 1.4031 steel Frame swivel holder POM, black *2 options for lids: Either position 2 or 4 **2 options for adapters: Either position 8 or 9 info@TSE-Systems.com www.TSE-Systems.com... -
Page 20: Air Supply Pump
Improper use of the pump can cause electrical shock or start a fire. • Repairs and maintenance work must be carried out by suitably qualified personnel only. • Always unplug the device from the mains before carrying out repairs and maintenance. info@TSE-Systems.com www.TSE-Systems.com... -
Page 21: Exchanging Filter
Check the air filters of the air supply pumps every month and replace them when dirty but LATEST every three months. Failure to do so will void the warranty. Fig. 3 Air pump (Top view) • Remove filter cover screw Fig. 4 Filter cover removed • Remove filter cover info@TSE-Systems.com www.TSE-Systems.com... -
Page 22: Overpressure Valve
The connection tubing can be wiped off with a disinfectant approved for plastics. Decontamination filter Every filter should be decontaminated before use. To decontaminate the filter, immerse it in disinfectant alcohol for 15min, then remove all alcohol traces by drying it in a fume hood. info@TSE-Systems.com www.TSE-Systems.com... -
Page 23: Chamber Block Change
18.000 running hours or LATEST after 36 months (Diaphragm and chamber block are one set) Filter change Every 3 months Recommended working pressure 120mbar (1.74psi) 150mbar (2.18psi) 150mbar (2.18psi) Operating pressure range 100…230mbar (1.45…3.34psi) 100…300mbar (1.45…4.35psi) 120…300mbar (1.74…4.35psi) info@TSE-Systems.com www.TSE-Systems.com... -
Page 24: Troubleshooting
Check pump by trained service personnel. Armature hits coil/core due to defective Check pump by trained service personnel. diaphragm. Fig. 6 Diaphragm protective switch deactivated. Fig. 7 Diaphragm protective switch activated (Pump powered Fig. 8 Diaphragm protective switch deactivated. OFF) info@TSE-Systems.com www.TSE-Systems.com... -
Page 25: Vacuum Pump
Select new protection air filters. • Wrap threads of both protection filter ends with sealing tape. • Slightly screw down the screw nuts to both filter ends and pass a tubing end through each of the screw nuts. info@TSE-Systems.com www.TSE-Systems.com... -
Page 26: Exchanging Disk Filter
Power OFF the complete system and disconnect the air pumps from the mains supply. • Push the inner two blue connector parts that are located close to the disk filter towards black connector parts. Fig. 9 Disassembling discus filter (1) • Remove used disk filter from system. info@TSE-Systems.com www.TSE-Systems.com... -
Page 27: Air Drying Unit
H2O2. The working life of such tubing material depends on the material itself, so that all tubing treated with H2O2 should be checked for damage at regular intervals. In order to eliminate impairment of the animals from exposure to H2O2, the H2O2 should be allowed to desorb for >=2.5 days, e.g., over a weekend. info@TSE-Systems.com www.TSE-Systems.com... -
Page 28: Replacing Flowmeter Battery
…but this depends on use. With constantly changing flow rates, the useful life may be shorter. We advise you to use only original battery modules. Please dispose battery in accordance with your local environmental regulations. Fig. 11 Replacement of flowmeter battery info@TSE-Systems.com www.TSE-Systems.com... -
Page 29: Calorimetry Measurement
Individual CaloSys module devices… …are described in Section 11.2 and following sections. Detailed information, particularly about safety measures, is given in the description for the individual instrument. If you are at all uncertain, particularly about safety-relevant measures, please contact Service. info@TSE-Systems.com www.TSE-Systems.com... -
Page 30: Push Principle Respirometry (Overpressure Principle)
Fig. 12 Schematic diagram of CaloSys module – Push principle respirometry – w/o Calibration Unit – Analog read-out – 8-place system Black lines: Cable connections Blue lines: Tube connections Lower green frame: Device used in combination with high-speed sensors only. info@TSE-Systems.com www.TSE-Systems.com... - Page 31 Separate Sensor Unit for fast O2 and CO2 measurements. For further instructions, see Hardware Operating Instructions - High-Speed Sensor Unit. In systems with a multiple of eight measuring places, further Slave Units are included in the system to connect up the additional calorimetry cages. info@TSE-Systems.com www.TSE-Systems.com...
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Page 32: Calosys Module To Be Operated With Automatic Or Manual Calibration
Fig. 13 Schematic diagram of CaloSys module – Push principle respirometry – w/ Calibration Control Unit – 8-place system Black lines: Cable connections Blue lines: Tube connections Lower green frame: Device used in combination with high-speed sensors only. info@TSE-Systems.com www.TSE-Systems.com... -
Page 33: Calosys Module Including Multiple Calorimetry Control Units
In the example (Fig. 14), an 8-place system includes a Calorimetry Master Control Unit containing one O2/CO2 sensor pair (i.e. one device) and one Calorimetry Slave Control Unit containing three O2/CO2 sensor pairs (i.e. three devices). Calorimetry cages are replaced by CaloTreadmills. info@TSE-Systems.com www.TSE-Systems.com... -
Page 34: Fig. 14 Schematic Diagram Of Calosys Module - Push Principle Respirometry - W/ Multiple Calorimetry Control Units - Digital Read-Out - 8-Place System
Fig. 14 Schematic diagram of CaloSys module - Push principle respirometry - w/ multiple Calorimetry Control Units - Digital read-out - 8- place system Black lines: Cable connections Blue lines: Tube connections *Treadmill Process Control Unit, CaloTreadmill cable connections not shown. info@TSE-Systems.com www.TSE-Systems.com... - Page 35 Connection to Calorimetry Master Control Unit via USB-RS485 converter box. Digital interface for CaloSys standard sensors. • Allows to switch between MFC mouse (1l/min) and MFC rat (5l/min) - if available. • "Treadmill" 37-pin cable connection to Treadmill Control Unit. info@TSE-Systems.com www.TSE-Systems.com...
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Page 36: Pull Principle Respirometry (Underpressure Principle)
Fig. 15 Schematic diagram of CaloSys module - Pull principle respirometry - w/o Calibration Control Unit - Analog read-out - 8-place system Black lines: Cable connections Blue lines: Tube connections Lower green frame: Device used in combination with high-speed sensors only. info@TSE-Systems.com www.TSE-Systems.com... - Page 37 Separate Sensor Unit for fast O2 and CO2 measurements. For further instructions, see Hardware Operating Instructions - High-Speed Sensor Unit. In systems with a multiple of eight measuring places, further Slave Units are included in the system to connect up the additional calorimetry cages. info@TSE-Systems.com www.TSE-Systems.com...
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Page 38: Calosys Module To Be Operated With Automatic Or Manual Calibration
Fig. 16 Schematic diagram of CaloSys module - Pull principle respirometry - w/ Calibration Control Unit - 8-place system Black lines: Cable connections Blue lines: Tube connections Lower green frame: Device used in combination with high-speed sensors only. info@TSE-Systems.com www.TSE-Systems.com... - Page 39 Separate Sensor Unit for fast O2 and CO2 measurements. For further instructions, see Hardware Operating Instructions - High-Speed Sensor Unit. In systems with a multiple of eight measuring places, further Slave Units are included in the system to connect up the additional calorimetry cages. info@TSE-Systems.com www.TSE-Systems.com...
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Page 40: Calosys Module Including Multiple High-Speed Sensor Units
...2 x 1 Sample Switch Control Units. • …2 x 4 Air Drying Units. • …1 x Calibration Master Control Unit. • …2 x 2 Calibration Slave Control Unit. • …2 x 4 High-Speed Sensor Units. • …1 x PC. info@TSE-Systems.com www.TSE-Systems.com... -
Page 41: Fig. 17 Schematic Diagram Of Calosys Module - Pull Principle Respirometry - W/ Multiple High-Speed Sensor Units - Digital Read-Out - 32-Place System
PhenoMaster | Hardware Operating Instructions Fig. 17 Schematic diagram of CaloSys module - Pull principle respirometry - w/ multiple High-Speed Sensor Units - Digital read-out - 32-place system Black lines: Cable connections Blue lines: Tubing connections info@TSE-Systems.com www.TSE-Systems.com... - Page 42 "Gas" Tube connection to Calibration Master Control Unit. "Unit 1...4" 3-pin connection to Calibration Master Control Unit. "Sample IN 1...8" Tube connection to Air Drying Unit1...8 via separating bottle. "Sample OUT 1...8" Tube connection to Calorimetry Control Units. info@TSE-Systems.com www.TSE-Systems.com...
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Page 43: Calorimetry Process Control Unit
In the Slave Control Unit, if necessary, there are connections for the temperature sensors "9...N", the fresh air supply (Flow OUT) for cages "9...N", and, depending on the CaloSys module configuration, one connection for the reference cage as well as an air pump connection. info@TSE-Systems.com www.TSE-Systems.com... -
Page 44: Connections Calorimetry Process Control Unit
NOTICE - Instrument damage! Measurement errors! Pressing the CO2 zero-point reset button can cause sensor damage and measuring errors. Do NOT operate red CO2 zero-point reset button on rear panel of Calorimetry Control Unit without prior consulting Service. info@TSE-Systems.com www.TSE-Systems.com... -
Page 45: Connections Calorimetry Master Process Control Unit
The animal cages "1...8" have to be connected to the "Flow OUT" outlets "1...8" via tubes. The reference cage inlet is connected to the "Flow Ref." connection. Fresh air supply: connect the "Flow IN" at the rear of the Control Unit via the T-piece and the pressure control valve to the air pump. info@TSE-Systems.com www.TSE-Systems.com... -
Page 46: Connections Calorimetry Master Process Control And High-Speed Sensor Unit
"O2/CO2" port that can be used for connecting a cable for data communication between the two Units. The sample air, which is led into the Calorimetry Master Control Unit´s "Sample IN" inlet, is led into the separate High-Speed Sensor Unit via the "Sample OUT" outlet. info@TSE-Systems.com www.TSE-Systems.com... -
Page 47: Connections Calorimetry Slave Process Control Unit
If the air is very humid, dirty, oily, or contains particles, this will affect the measuring accuracy of the MFC. Only work with the air pump supplied. Do NOT use oil driven compressed air generators to supply the air flow. info@TSE-Systems.com www.TSE-Systems.com... -
Page 48: Fig. 22 Flowmeter
1bar (14.5psi) MFC calibration The calibration parameters of the MFCs are set in the factory and should be changed after consulting TSE Service only. For checking the flow produced by the MFCs, a flowmeter can be connected to the tubing leading, e.g., to the Flow IN inlet of the cage (push system). -
Page 49: Setting Sample Flow On Calorimetry Master Control Unit
Fig. 23 Rotary knob MFC sample flow at the Calorimetry Control Unit Locking of sample flow setting The MFC is adjusted by TSE Service during installation according to the CaloSys module configuration (rat/mouse). After a calibration, the setting should NOT be altered, as otherwise the calibration will no longer be accurate and a new calibration procedure should be carried out. -
Page 50: Co2 Sensor - Working Principle
• Do NOT immerse the sensor in water, cleaning liquids, etc. Cage temperature sensor precalibrated in the factory. 11.2.5.1. Analogous Temperature Sensor It is a precision integrated-circuit temperature sensor, whose output voltage is linear proportional to the temperature. info@TSE-Systems.com www.TSE-Systems.com... -
Page 51: Digital Temperature Sensor
Unit connect to the Calibration Slave Control Units (see Section 11.3.2). "XOext." connects to the Calorimetry Master Control Unit. CaloSys module retrofitted for automatic calibration (hardware upgrade): "X0ext." of Calibration Master Control Unit (Fig. 26) connects to the PC. Fig. 24 Calibration Master Control Unit - Front panel info@TSE-Systems.com www.TSE-Systems.com... -
Page 52: Fig. 25 Gas Pressure Display
Calibration gas2 pressure (Gas Inlet2) Calibration gas3 pressure (Gas Inlet3) Operating of gas pressure display buttons… …is reserved for TSE Service only! Fig. 26 Calibration Master Control Unit - Rear panel USB connection to PC. Tube connection to sample pump of the Air Drying Unit via separating bottle. -
Page 53: Connections Calibration Slave Process Control Unit
"Gas" connect to the Calibration Master Control Unit. "Sample IN" connects to the sample switch pump via Air Drying Unit. "Sample OUT" connects to the "Sample IN" of the Calorimetry Control Unit. Fig. 27 Calibration Slave Control Unit Tube connection to Calibration Master Control Unit. 3-pin connection to Calibration Master Control Unit. info@TSE-Systems.com www.TSE-Systems.com... -
Page 54: Sample Switch Unit
For drawing the reference air and samples from the animal cages, each of them has to be connected to the sample air inlets ("1...N") on the rear panel of the Sample Switch Unit via tubing. The 25-pin port should be linked to the "X04" port on the rear panel of the Calorimetry Master Control Unit. info@TSE-Systems.com www.TSE-Systems.com... -
Page 55: Information About Reference Air
• Always run system software when devices are powered ON. • Power OFF devices after use. The air supply pump allows to generate and maintain the set airflow within the CaloSys module. info@TSE-Systems.com www.TSE-Systems.com... -
Page 56: Fig. 30 Air Supply Pump
An integrated diaphragm protective switch is included in the following models: • AP-60 • AP-80 An integrative thermal protective switch (resettable) is included in the following models: • AP-30 (trigger temperature: 135°C/275°F) • AP-40 (trigger temperature: 135°C/275°F) • AP-60 (trigger temperature: 100°C/212°F) • AP-80 (trigger temperature: 100°C/212°F) info@TSE-Systems.com www.TSE-Systems.com... -
Page 57: General
Before commissioning, ensure that pressure control valves in the tubing are opened - if relevant. 11.6.3. Connections DANGER Hazardous voltage Improper installation can cause electrical shock or start a fire. If the mains cable is damaged, repairs and maintenance work must be carried out by suitably qualified personnel only. info@TSE-Systems.com www.TSE-Systems.com... -
Page 58: Operation
When cleaning, ensure that no water or any other liquids enter the valve. Tubing connections… …must be safeguarded with tubing clamps or other safety devices, etc. to prevent them from being accidentally loosened. 11.6.4. Operation • Ensure that the operating air temperature is within the range -10...+40°C (14…104°F) info@TSE-Systems.com www.TSE-Systems.com... -
Page 59: Vacuum Pump
Choking the air flow by reducing the outlet or closing it leads to premature wear of the pump. If the air flow fails, the windings overheat and burn. • Unusual noises may indicate that a diaphragm has broken. In this case, unplug device from mains supply and contact Service. Fig. 32 Vacuum pump info@TSE-Systems.com www.TSE-Systems.com... -
Page 60: Installation
Before commissioning, ensure… • …that pressure control valves in the tubing are open - if relevant. • …that you should not be able to notice loud noises or strong vibrations. info@TSE-Systems.com www.TSE-Systems.com... -
Page 61: Connections
The opening for the pressure release of the pressure control valve must not be allowed to become dirty, as this could limit or prevent its functioning. • Only operate a pressure control valve that isn´t dirty. • When cleaning, ensure that no water or any other liquids enter the valve. info@TSE-Systems.com www.TSE-Systems.com... -
Page 62: Operation
Observe the instructions about the flow direction of the filter units in the following description. Insert the filters into your CaloSys module by following these steps: • Power OFF the complete system and disconnect the air pump from the mains supply. info@TSE-Systems.com www.TSE-Systems.com... -
Page 63: Fig. 34 Connections Protection Air Filter
Push the tubing ends over the two filter connections and then screw down the screw nuts tightly so that the tubing cannot become loosened accidentally. Filter installation Ensure that the filter is inserted in the correct flow direction. There is a flow direction arrow on the filter label. Fig. 35 Flow direction info@TSE-Systems.com www.TSE-Systems.com... -
Page 64: Air Drying Unit
Air Drying Unit is operated at a temperature of 5°C (41°F). Do NOT alter the temperature setting. Control panel Reserved for TSE Service only. Do NOT operate the buttons below the display, as this could cause malfunction of the device. -
Page 65: Air Drying Unit
After you have discarded condensed water, carefully reconnect collection vessel to 1x Air Drying Unit and reconnect separating bottle to tubing. • Select "Status/Calo" and check for proper "SampleFlow” on "Calo Current Values" dialog (see Software Operating Instructions – Calorimetry, Section "Status Menu, Calo"). info@TSE-Systems.com www.TSE-Systems.com... -
Page 66: Air Drying Unit
If Calorimetry Process Control Units and Air Pumps are powered ON while there is a leakage in the sample line, this may reduce lifetime of devices. • Never remove collection vessels from the 4x Air Drying Unit. Drain off condensed water via collection vessel tubing only. info@TSE-Systems.com www.TSE-Systems.com... -
Page 67: Connections Air Drying Unit
The sensors in the Calorimetry Control Unit are sensitive measuring instruments that must be protected against humidity. There should be no condensed water in the tubings via the O2 and CO2 sensors. Check the tubing regularly and discard any condensed water. info@TSE-Systems.com www.TSE-Systems.com... -
Page 68: Appendix
CaloSys Control Unit CaloSys Control Unit for Valves Air Drying Unit CaloSys Calibration Unit Treadmill Control Unit Treadmill Total: 2565 *Under normal operation conditions. Power On peak is higher and can be calculated from max. current (fuse) and voltage. info@TSE-Systems.com www.TSE-Systems.com... - Page 69 CaloSys Modules info@TSE-Systems.com www.TSE-Systems.com...
- Page 70 Sophisticated Life Science Research Instrumentation https://www.tse-systems.com/support/technical-support-form/ Specifications subject to change without notice Copyright © 2017 TSE Systems International Group - All rights reserved www.TSE-Systems.com info@TSE-Systems.com...
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