MLT FS-9-25 Instructions For Use Manual
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MLT FS-9-25 Instructions For Use Manual

Automated slide stainer
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Automated Slide Stainer FS-9-25
Instruction for Use
Read carefully before working with the stainer
Always keep this Instructions for Use near the stainer
V1.1 English – 10.02.2021
Dubna 2021

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  • Page 1 Automated Slide Stainer FS-9-25 Instruction for Use Read carefully before working with the stainer Always keep this Instructions for Use near the stainer V1.1 English – 10.02.2021 Dubna 2021...
  • Page 2 Revision history Revision Effective date Change Written by 22.01.2021 Draft Bezrukov A.V. Kutepov M.V. 10.02.2021 For CE marking Bezrukov A.V.
  • Page 3 ALWAYS KEEP THIS INSTRUCTION FOR USE NEAR THE STAINER. MLT LLC reserves the right to change technical specifications as well as manufacturing processes without prior notice. Only this way is it possible to continuously improve the technology and manufacturing techniques. The information on important changes is added to the technical file of the device and is published on the manufacturer’s website www.mlt.ru.

  • Page 4: Table Of Contents

    Table of contents 1 Important information ......................5 1.1 Used symbols and indications ..................5 1.1.1 Warning words..................... 5 1.1.2 Symbols....................... 6 1.1.3 Stainer label ......................7 1.1.4 Designation of menu sections, touch screen buttons, and operational actions..8 1.2 Intended use .......................
  • Page 5 3 Safety measures when handling HFL and CL ............. 55 4 Actions in case of fire ....................56 APPENDIX B. LIST OF REAGENTS FS-9-25* ..............57 APPENDIX С. Example of developing the <DEMO> program ........... 58 Protocol of the <DEMO> program and its configuration ..........58 Listing of the <DEMO>...

  • Page 6: Important Information

    This instruction for use includes important information related to the operating safety and maintenance of the automated slide stainer FS-9-25 (further – Stainer). The instruction for use is an important part of the product. It must be read carefully and completely before setup and prior use of the Stainer and must be kept near the device at all times.

  • Page 7: Symbols

    1.1.2 Symbols. Symbol Name of the symbol Location, designation Lid of the Stainer. A warning about handling of Warning, danger toxic and corrosive reagents, about the necessity to use protective equipment. Lid of the Stainer. A warning about the handling of Fire hazard highly flammable liquids.

  • Page 8: Stainer Label

    Open here Operational documentation. Instruction for use, label on the rear panel of the Stainer, labels on the packing box of the device. A MLT LLC logo stylized image of the manufacturing company’s name. 1.1.3 Stainer Label The label containing the Stainer’s name, its serial number, the year of manufacture and other information is situated at the rear panel of the device.

  • Page 9: Designation Of Menu Sections, Touch Screen Buttons, And Operational Actions

    1.1.4 Designation of menu sections, touch screen buttons, and operational actions. Menu sections are designated with capital letters between double angle quotation marks, e.g., «PROCESS», «SERVICE», «CONFIGURATION», etc. Touch screen buttons are designated in the text as inscriptions in capital letters placed in a frame, e.g., ENTER, YES, STOP.

  • Page 10: Intended Use

    1.2 Intended use The automated slide stainer FS-9-25 is a device for IN VITRO diagnostics. The device is designated for staining of biological preparations on slides, and it can be used for morphological examinations in hematology; microbiology; parasitology; cytology (only with Romanowsky type staining).

  • Page 11: Safety

    2 Safety 2.1 General safety statements This Stainer has been developed, manufactured and tested under safety requirements for electrical laboratory devices. To maintain a safe technical state of the Stainer, the operator must observe all requirements of this instruction for use. If the instructions are not followed, then the protection provided by the device may be impaired.
  • Page 12 hood. FORBIDDEN TO OPERATE THE STAINER, IF IT IS NOT CONNECTED TO VENTILATION OR BEYOND EXHAUST HOOD. Highly flammable liquids, such as spirits, spirit-based dyes, xylene or xylene substitutes may be used during operation. FORBIDDEN TO INSTALL THE STAINER IN DIRECT VICINITY OF A FLAME OR IGNITABLE SPARKS.

  • Page 13: General Characteristics

    3. General characteristics 3.1 Description of Stainer 3.1.1 Construction of Stainer. The Stainer consists of a technological unit (5) with a working chamber and a lid (1) and a control unit (3) with a touch-screen (2). Figure 2. Front view of the Stainer. 1 –...
  • Page 14 Figure 3. Rearview of the Stainer. 1 – transparent lid; 8 – tap water inlet; 9 – screw-type foot; 10 – exhaust ventilation grid with an outlet*; 11 – Stainer label with a serial number, manufacturing year; 12 – protective cover for 10 A electric fuses; 13 – power supply inlet; 43 – tap water outlet. * An inflow ventilation inlet, protected by a removable lid and a filter, is situated on the bottom of the Stainer.
  • Page 15 Figure 4. Top view of the Stainer, working chamber of the technological unit. 14 – drying station; 15 – arm of manipulator; 16 – trough with a rack inside (parking station); 17 – rack with slides; 19 – manipulator housing; 20 – upper panel of Stainer; 21 – flow-through trough;...

  • Page 16: Accessories: Troughs And Racks

    3.1.2 Accessories: troughs and racks. The Stainer is supplied with RV-25 racks for 25 slides. The Stainer is supplied with two types of troughs: TV-25-PP – a polypropylene trough (color: either white or natural half- transparent) for RV-25 racks; recommended when handling aqueous solutions; spirits; and acetone.

  • Page 17: Micro Sd Card And Micro Sd Card Slot

    3.1.3 Micro SD card and micro SD card slot. The Stainer is supplied with micro SD card, and the external micro SD card slot (Figure 2, p 4). The memory card is used to store microprocessor firmware data. The firmware can be updated or edited using the software for PC "Fastainer Firmware Editor" (APPENDIX F).
  • Page 18 technological operation can be performed in a number of ways: immersion into the reagent, immersion with a programmed period of activation, repeated dipping of slides into the reagent (can accelerate the speed of procession). When processing of a rack is complete, it goes back to the initial station, where it has been loaded in the beginning*—...

  • Page 19: Technical Data

    3.2 Technical data Number of combined stations Number of drying stations Number of washing stations (with a flow-through trough) 76  26 х 1,0 mm Size of slides* Stained part of a slide ~ 26 × 55 mm Capacity of RV-25 racks Heating modes of the drying station strong/weak Ventilation of the working chamber...

  • Page 20: Delivery Set

    3.4 Delivery set A standard delivery set includes: Product code Quantity Device 266012.007.9 Automated slide stainer FS-9-25 Accessories RV-25 rack for slides (for 25 slides) 06.01.00 TV-25-PP trough for reagents with a lid 06.06.00 Kit for tap water connection, 05.70.00 including: - Нose for water connection...

  • Page 21: Unpacking And Installation Of Stainer

    4. Unpacking and installation of Stainer 4.1 Site requirements The Stainer must be placed on a solid horizontal surface with the dimensions min. 0,6 m long and 0,6 m wide. A grounded socket for connection to power must be situated not father from the installation site than the length of the cable (1,5 m).
  • Page 22 IT IS RECOMMENDED TO KEEP THE ORIGINAL PACKING OF THE STAINER AND ACCESSORIES. CAUTION! ANY DEVICE, ITS PART, OR ACCESSORY, WHICH IS RETURNED TO MLT LLC, OR WHICH IS IN NEED OF MAINTENANCE AT THE OPERATION SITE, MUST BE APPROPRIATELY CLEANED AND DISINFECTED. 5.4.2)

  • Page 23: Connection Of Stainer

    4.3 Connection of Stainer Place the Stainer on the installation site. If necessary, align the device in a horizontal plane with the help of screw-type feet (9) (Fig. 3) 4.3.1 Electric connection. The Stainer is connected to the electric socket with a power cord, which is supplied with the device.
  • Page 24 and dishwashers. When connecting to the sink siphon pipe stub, fix the hose with a clamp. CAUTION! BEFORE CONNECTION, CHECK FOR A BACK-FLOW VALVE AT THE PIPE STUB OF SIPHON; IF THERE IS ONE – REMOVE IT, BECAUSE THE WATER DRAIN, FLOWS NATURALLY. CAUTION! THE DRAIN HOSE MUST NOT BE KINKED, BENT, OR PINCHED CAUSING HOSE DAMAGE OR RESTRICTING WATER FLOW.
  • Page 25 CAUTION! MAKE SURE THERE ARE NO LEAKS IN THE WATER SUPPLY AND IN THE DRAIN SYSTEMS AND THAT THERE ARE NO BENDS AT THE DRAIN HOSE BEFORE STARTING THE OPERATION. CAUTION! WHEN THE WORK IS FINISHED, IT IS RECOMMENDED TO TURN OFF THE TAP.

  • Page 26: Operation And Control Of Functioning

    5. Operation and control of functioning 5.1 Main menu, menu structure Operation and functioning control of the Stainer is carried out with a 480 x 272-pixel color touch screen. When the Stainer is switched on, it starts self-testing, and a startup screen with a manufacturer’s (or dealer’s) logo appears on the touch screen (Fig.

  • Page 27: Menu Section "Programming". Programming Of New Techniques, Editing Of Parameters Of Previously Downloaded Techniques

    troughs with reagents in the working chamber following the protocol of technological program, and check the expiry date of reagents. The bottom part of the screen has the following buttons: TECHNIQUES START OF PROCESS CONFIGURATION TECHNIQUES – to switch to the menu section «PROGRAMMING» (choice of a technological program from the available ones, correction of parameters of a program and programing a new technological program).
  • Page 28 a respective name. Pressing the button DEMO starts a demonstration program for acquaintance with the Stainer and checks of its working capacity. The bottom part of the screen has the following buttons: MAIN MENU ◄► MAIN MENU – to switch back to the main screen. ◄►...
  • Page 29 Enter the name of the new program and press the button ENTER to switch to the screen of program editing. If the entered name is unique, i.e., it has not been entered before, a template of a new program (Fig. 14) with the already given number of operations appears on the screen.
  • Page 30 - Set an operational <MODE> for the manipulator (<IMMERSION> or <DIPPING>). To change the mode, touch the screen at the spot of the needed parameter. Mode <IMMERSION>. The mode, in which a rack with slides is immersed into the reagent and is kept in it until the end of a technological operation. Mode <DIPPING>.
  • Page 31 Figure 16. NEW PIN entering screen. PIN is useful to prevent unauthorized program editing. It is possible to choose any 3-digit PIN in the range 000-999. After entering a NEW PIN, press the button ENTER, and the Stainer will return to the main menu. CAUTION! A CONFIRMATION IS OBLIGATORY FOR SAVING A NEW PROGRAM AND FOR SAVING CHANGES.
  • Page 32 The Stainer lets you program and save up to 32 programs. All programs, including the preprogrammed ones (factory programs), can be edited. An edited program can be saved under a new name (as a new program), herewith a message will appear on the screen: <SAVE CHANGED PROGRAM UNDER A NEW NAME?>...

  • Page 33: Menu Section "Configuration". Programming And Control Of The Configuration, Performing Of Service Functions

    troughs with reagents. To develop a new program, use the special form, the template for which is given in Appendix C. An editable file with template can be uploaded at the website www.fastainer.com in the “support” section. There is also an opportunity to write a new program with special personal computer software «FASTAINER FIRMWARE EDITOR»...
  • Page 34 The format of buttons: NN RESOUR ХХХ STATION APPLICATION where: <NN> – number of station, <STATION APPLICATION> – application of station, <RESOUR ХХХ> – how many times it is left for a manipulator to revert to a station with reagent until it has to be changed (resource limit of a reagent), (the limit is set by the operator within the interval 0-255, default value is 255).
  • Page 35 < STATION APPLICATION > parameter < DRYING > – not editable. <REAGENT> parameter <AIR> – not editable. <MODE> parameter <HEATING++> – modes of heating of air in the drying station. Pressing the button switches between modes <HEATING+> (weak) and <HEATING++> (strong). At the mode <HEATING+>...
  • Page 36 Figure 22. Configuration of station No. 3 with reagent. <STATION APPLICATION> parameter <TECHNOLOGICAL> – editable parameter. The station is reserved for a trough with reagent. On pressing the button TECHNOLOGICAL, the value is switched to <PARKING>, which means that the station is now designated for placement of racks.

  • Page 37: Setting The Interval Of The Introduction Of Racks Into The Process

    <DELAY> – editable parameter: the duration of a rack's delay in the upper position after rising from a trough (0-99 seconds, the default value is 10). The set values are saved at returning to a previous screen after pressing the button CANCEL.

  • Page 38: Menu Section "Service". Performing Of Service Functions

    CAUTION! WHEN CALCULATING AN OPTIMAL INTERVAL, IT IS VITAL TO TAKE INTO CONSIDERATION THAT THE TIME, SPENT FOR TRANSFERRING A RACK AND FOR RACK’S DELAY UNDER A TROUGH FOR DRAINING OF LIQUID, IS NOT ADDED TO THE VALUE OF THE INTERVAL BY DEFAULT. Before starting a new program with real reagents, it is necessary to check that the interval has been calculated correctly by running the program with empty racks.
  • Page 39 accessories and manipulator. The test program is recommended for the commissioning of the Stainer (Item 6.3) and the identification of faulty accessories. WATER SUPPLY – PROGRAMMED  WATER SUPPLY – CONTINUOUS – is intended to set the mode of water supply into the flow-through trough. - <PROGRAMMED>...

  • Page 40: Menu Section "Process". Loading And Unloading Of Racks. Processing Of Racks With Slides According To A Given Technological Program

    5.4 Menu section «PROCESS». Loading and unloading of racks. Processing of racks with slides according to a given technological program To access the menu section «PROCESS», press the button START OF PROCESS in the Main menu. 5.4.1 Loading of racks. After pressing the button START OF PROCESS, the Stainer offers to load racks with slides, and a message appears on the screen: Figure 25.
  • Page 41 Figure 26. Loading and unloading of a rack. 15 – arm of the manipulator; 17 – rack; 18 – handle of rack; 25 – grab of the manipulator; 27 – index plate of stations numeration; 32 – loop of manipulator grab. Continuation of loading of racks.

  • Page 42: Loading Of Racks During The Performance Of A Technological Program

    When a technological program is in progress, stations and their state are shown on the screen. Figure 27. Status screen of the <DEMO> program. 5.4.2 Loading of racks during the performance of a technological program. To load more racks during an ongoing process, press the button UPLOAD (Fig. 26).

  • Page 43: Unloading Of Racks

    (technique), and returns to the loading position above station No. 3, herewith a message appears on the screen again: <PUT THE RACK IN THE MANIPULATOR AND PRESS THE BUTTON <CONTINUE> - TO UPLOAD ONE MORE RACK <FINISH> - TO FINISH THE UPLOADING> Racks can be loaded to unoccupied parking stations until the Stainer configuration for the used technological program allows them to do it.

  • Page 44: Status Screen During A Technological Process

    processing of all of the racks is complete, a message appears on the screen accompanied by a signal. <AFTER REMOVING OF THE RACK PRESS THE BUTTON <CONTINUE> When all of the racks have been unloaded, the Stainer switches back to the main menu.
  • Page 45 Display format of buttons for technological stations during standby: NN RESOUR ХХХ REAGENT where: <NN> – number of station, <REAGENT> – reagent, according to the programmed configuration, <RESOUR ХХХ> – how many times it is left for the manipulator to revert to a station with reagent until it has to be changed (resource limit of a reagent), (the limit is set by the operator within the interval 0-255, default value is 255).
  • Page 46 Buttons for parking stations. Display format of stations reserved for a rack (WHITE COLOUR) (Fig. 27, stations No. 9): PARKING Here and further <NN> – number of stations. Display format of stations with a loaded unprocessed rack (YELLOW COLOUR) (Fig. 28, station No. 9): RACK Display format of stations, the rack of which is being processed (ROSE COLOUR) (Fig.

  • Page 47: Preparing For Operation

    6. Preparing for operation FORBIDDEN TO SWITCH ON THE STAINER WITHOUT PRIOR READING THE INSTRUCTION FOR USE. CAUTION! BEFORE SWITCHING ON THE STAINER, MAKE SURE THAT THE WORKING CHAMBER DOES NOT CONTAIN ANY EXTRANEOUS ITEMS, THE TROUGHS ARE ARRANGED IN THEIR PLACES. 6.1 Switching on Switch on the Stainer with the power switch (6) at the right panel.
  • Page 48 A message will appear on the touch screen: PUT THE RACK HANDLE INTO THE GRAB OF MANIPULATOR AND TOUCH THE SCREEN TO EXIT, PRESS <CANCEL> CAUTION! POSITIONING TEST IS PERFORMED WITH AN OPEN LID. NO EXTRANEOUS OBJECTS (INCLUDING OPERATOR’S HANDS) MUST IMPEDE THE MOVING OF MANIPULATOR.
  • Page 49 6.2.2 Check of manipulator’s movement locking. Switch on the Stainer, consecutively press the following buttons, starting from the main menu: TECHNIQUES  DEMO  ENTER  START OF PROCESS The Stainer will start performing a demonstrational program <DEMO>. When the manipulator is moving, press the button STOP. The movement must stop.

  • Page 50: Work Sequence

    7. Work sequence 7.1 Before operation Comply with the recommended safety statements ( Chapter 2). Check the connection of Stainer to electrical, ventilation, water supply, and drain systems. Make sure that the Stainer is installed strictly horizontally. Check the arrangement of troughs in the Stainer and that the troughs are not covered with lids.

  • Page 51: Cleaning, Disinfection, And Maintenance

    If the Stainer has been used with materials that are toxic or contaminated with pathogenic micro-organisms, follow the cleaning instructions given in Chapter 8. The product return disinfection certificate Chapter 11 must be completed if the instrument is to be returned to MLT LLC.

  • Page 52: Cleaning And Disinfection Of Outer Surfaces And The Working Chamber

    8 Cleaning, disinfection, and maintenance 8.1 Cleaning and disinfection of outer surfaces and the working chamber Outer surfaces and the working chamber of the Stainer are cleaned and disinfected with a soft cloth and a cleansing disinfecting agent once a week or more often, depending on the pollution intensity.

  • Page 53: Mesh Water Filter

    8.5 Mesh water filter Mesh filter of the connection hose prevents the water supply system from gumming up. Mesh filter is cleaned once it gets dirty. Mesh filter is situated inside the screw part of the tap water inlet (8) (Fig. 3) and can be accessed from the outside of the Stainer for cleaning (a supplemental mesh filter might also be inside the connection hose).

  • Page 54: Troubleshooting

    9 Troubleshooting Error Possible cause Way of elimination The Stainer does not 1 The fuses have blown. 1 Change the fuses. The fuses can be switch on. changed only when the Stainer is switched off (plug of the power cord must be disconnected from the socket).

  • Page 55: Confirmation Of Performed Sanitary Treatment

    11 Confirmation of performed sanitary treatment CAUTION! ANY DEVICE, ITS PART, OR ACCESSORY, WHICH IS RETURNED TO MLT LLC, OR WHICH IS IN NEED OF MAINTENANCE AT THE OPERATION SITE, MUST BE APPROPRIATELY CLEANED AND DISINFECTED. template disinfection certificate available website www.fastainer.com/resources.

  • Page 56: Appendix A. Instruction On Fire Safety Measures

    1.1 The present instruction has been composed to ensure fire safety when operating the automated slide stainer according to TU 266012-007-23475651-2018 in the following models: FS-9-25, FS-9-25 (further – Stainer). Some technological operations, executed with the Stainer, require the usage of highly flammable liquids (HFL) and combustible liquids (CL): methanol, ethanol, xylene, etc.

  • Page 57: Actions In Case Of Fire

    3.5. It is forbidden to carry out any works in the exhaust hoods if there are substances, materials and equipment, not relating to the performed operations, as well as when an exhaust hood is defective, or the ventilation system is switched off. 3.6.

  • Page 58: Appendix B. List Of Reagents Fs-9-25

    APPENDIX B. LIST OF REAGENTS FS-9-25* REAGENT REAGENT REAGENT REAGENT ACETIC ACID AZURE FLUOROCHROME SAFRANIN ACETONE AZURE-EOSIN FUCHSIN SOLUTION-1 ACID SOLUTION BLOTTER GENTIANVIOLET SOLUTION-2 ALCOHOL BUFFER GIEMSA SOLN. SOLUTION-3 ALCOHOL-1 BUFFER РН<7 HCL ACID STAIN-1 ALCOHOL-2 BUFFER РН~7 HEMATOXYLIN STAIN-2 ALCOHOL-3 BUFFER РН>7...

  • Page 59: Appendix С. Example Of Developing The Program

    APPENDIX С. Example of developing the <DEMO> program Protocol of the <DEMO> program and its configuration DEVICE: FS-12-25 NAME OF PROGRAM: DEMO DATE: 15.01.2019 REAGENT CONFIGURATION FS-9-25 REAGENT PARKING TAP WATER PARKING BUFFER PH<7 PARKING AZURE LEISHMAN F-S EOSIN REGIME...

  • Page 60: Listing Of The Program And Its Configuration

    Listing of the <DEMO> program and its configuration The first screen of the program listing Second screen of the program listing Configuration screen...

  • Page 61: Template For Developing A New Technological Program

    Template for developing a new technological program DEVICE: FS-9-25 NAME OF PROGRAM: DEMO DATE: REAGENT CONFIGURATION FS-9-25 REAGENT TAP WATER REGIME AGITATION TIME DELAY RESOURCE № REAGENT (I, D) ММ:SS...

  • Page 62: Appendix E. List Of Adopted Terms And Abbreviations

    LIST OF ADOPTED TERMS AND ABBREVIATIONS Combined station – a station that can be used, both as a parking and as a technological one, depending on the configuration of the Stainer. The FS-9-25 has combined stations No. 3 to 9.
  • Page 63 Technological operation - a set of sequential actions with a rack with slides within the same station (lowering, processing slides in the reagent, taking out, time-exposure for reagent drain). Technological program - a set of sequential technological operations that ensure the preparation staining according to a given technique.

  • Page 64: Appendix F. "Fastainer Firmware Editor" Pc Software

    APPENDIX F. «FASTAINER FIRMWARE EDITOR» PC SOFTWARE User manual for the program «Fastainer Firmware Editor» The program «Fastainer Firmware Editor» is designed to edit the firmware file for the automated stainer Fastainer-12-25. The program allows to delete copy and edit technological programs available in the firmware file, create new technological programs, edit the list of user reagents, and select the optimal intervals for launching racks for processing.
  • Page 65 selected technology program (name, an interval for the launching of the new rack, and pin code). 1.1 Buttons of the Main Menu (2) Firmware. Load defaults – Resets the firmware to the factory state Open – Opens the firmware file Save –...
  • Page 66 1.3 «Operations» (4) – the list of technological operations in the selected technological program. Operations (1-30) – Button for adding of new technological operation. – Button for deleting technological operation. Operations can be swapped using the Drag and Drop method. 1.4 <Operation>...
  • Page 67 1.9 – «Program» (9) - setting of the selected technology program. The setting of the name, launching interval of the racks, and pin code of technological program are fulfilled in this section. 2 The window «Chart» of the software Figure 31. The window «Chart» of the software 1 –...
  • Page 68 3 Window for optional reagents setting Figure 32. The window «Reagents» of the software 1 – Logotype. 2 – Main Menu. 12 – List of default reagents. 13 – New reagent input bar. 14 - List of optional reagents. The reagent used in any of the technological programs is undeletable.
  • Page 69 4 Stainer firmware reloading with SD-card 4.1 and SD card requirements: - SD card capacity: 512 MB to 32 GB - SD card file system: FAT32; allocation unit size (cluster size): 32 KB 4.2 The procedure for reloading of the firmware using an SD card: Switch on the Stainer.

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