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Hyperspectral Imaging System CM401 User Guide...
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The software may be used or copied only in accordance with the terms of those agreements. No part of this publication may be reproduced, stored in a retrieval system, or transmitted in any form or any means electronic or mechanical, including photocopying and recording for any purpose other than the purchaser's personal use without the written permission of ThorLabs, Inc.. ThorLabs, Inc.
3.1. Features at a Glance 3.2. Specifications Overview Chapter 4 Getting Started 4.1. Unpacking and Inspection 4.2. Setting up the CM401 4.2.1. General Setup 4.2.2. System Installation Chapter 5 Capturing an Image Chapter 6 Maintaining the CM401 6.1. Cleaning Chapter 7 Specifications 7.1.
Chapter 1: CM401 Warning Symbols and Definitions Chapter 1 Warning Symbols and Definitions Below is a list of warning symbols you may encounter in this manual or on your device. Symbol Description Direct Current Alternating Current Both Direct and Alternating Current...
Any modification or servicing of this system by unqualified personnel renders Thorlabs free of any liability. Only personnel authorized by Thorlabs and trained in the maintenance of this equipment should remove any covers or housings or attempt any repairs or adjustments.
Chapter 3: CM401 Description Chapter 3 Description Thorlabs' Hyperspectral Imaging System provides a platform to study a sample with co-located features. In hyperspectral imaging, a stack of spectrally separated, two-dimensional images is acquired. This allows quick sample identification and analysis. ®...
Chapter 3: CM401 Description 3.2. Specifications Overview The following table provides an overview of the CM401 specifications. For detailed specifications on individual devices, see Specifications Chapter on Page 17. Specification Value Microscope Cerna Series Modular Microscope Objective Nikon 10X Plan Fluorite Objective (N10X-PF) Condenser 0.9 NA Air Condenser (CSC1002)
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Chapter 3: CM401 Description Specification Value Light Source Specifications HPLS343 Item# High-Power Plasma Light Source and Ø3 mm LLG (Liquid Type Light Guide) 350–800 nm Wavelength Range 4.0 W (Typical) 3.5 W (Minimum) Optical Output Power Automated Stage Specifications 1" (25.4 mm) Motorized Travel Nosepiece and Condenser Holder 1"...
This section is provided for those interested in getting the Hyperspectral Imaging System up and running quickly. 4.1. Unpacking and Inspection Open the package, and carefully remove the CM401 and its accessories. The table lists the standard accessories shipped with the device. Name Quantity...
For optimal image acquisition, we strongly advise that the following standard practices are met: ● Acquire images using actively isolated or anti-vibrational table platforms. Failure to utilize appropriate platforms results in poor quality images. Thorlabs offers a wide array of optical tables and platforms that provide the stability for your imaging needs.
Chapter 4: CM401 Getting Started 4.2.2. System Installation CAUTION DO NOT install the Hyperspectral Imaging System by yourself. Only an authorized personnel should install this system. A. Mounting the System Mount the microscope body onto the optical table. Remove the shipping plates on the microscope body and rigid stand.
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Chapter 4: CM401 Getting Started Mount the rigid stand onto the optical table. Mount the MCM3000 Controller Boxes(2X) onto the optical table. B. Computer Please refer to the computer's user manual for installation and operating instructions. ThorImageLS 3.1 software comes preinstalled on the computer. Install the ThorCam camera software on the computer (see the relevant user guide for details).
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Chapter 4: CM401 Getting Started C. Cable Connections Auxiliary Cable Optical Head Cable Filter Controller (Back View) Liquid Light Guide CM401 TSI-IOBOB HPLS343 Computer MCM3000 MCM3000 USB Cable Gigabit Ethernet Cable Connect the 3 Axis Controllers to the MCM3000 Controller Boxes.
Chapter 5: CM401 Capturing an Image Chapter 5 Capturing an Image Make sure to install the required software before you start the experiment. Open ThorImageLS application on the computer. Select the desired devices from the Hardware Configuration window. The following image shows the standard devices required for Hyperspectral system. Wait till a green dot appears next to all the selected devices, and then click OK.
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Chapter 5: CM401 Capturing an Image In the Kurios Filter panel, set the value for Start, Stop, and Step Size. You can set the Start and Stop value between 420–730 nm. In the below image, the Start value is set to the minimum wavelength (420 nm) of the KURIOS filter.
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Chapter 5: CM401 Capturing an Image Enter the Stop value as the Wavelength value (for example, 730 nm). In the Camera Control panel, change the Exposure value until you see the desired brightness on the image. Record the desired Exposure value (Stop Exposure) on a paper.
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Chapter 5: CM401 Capturing an Image In the above example, 11 different wavelengths have been recorded. The hyperspectral acquisition uses the graph as reference to get the exposure corresponding to each wavelength. In the tab, select Hyperspectral for Capture Mode.
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Chapter 5: CM401 Capturing an Image Click to start the acquisition. Click tab to load the captured experiment. A new slider appears that shows the spectral wavelength of the current image and its index. For information about the hyperspectral acquisition and camera settings, expand the Info panel.
CM401 Maintaining the CM401 Chapter 6 Maintaining the CM401 The unit does not need regular maintenance. If you suspect a problem with your CM401, please contact our nearest office for assistance from an application engineer (see Thorlabs Worldwide for details).
Chapter 7: CM401 Specifications Specification Value Oblique Darkfield 1 Slot (Not Included) Phase 3 Slots for Phase Annulus (Not Included) a. N1 and N2 condenser prisms should be paired with a compatible dry objective. 7.6. Tunable Bandpass Filter Specifications Specification...
Chapter 7: CM401 Specifications d. The power density of your beam should be calculated in terms of W/cm. 7.7. Camera Port Specifications Specification Value Magnification Dovetail Male D1N Tube Lens Focal Length 200 mm Camera Threading C-Mount (1.00"–32) 7.8. Camera Specifications...
Chapter 7: CM401 Specifications Specification Value Full Sensor, Bin by 2 (1024 x 1024) 7.5 fps 13.1 fps 12.8 fps 20.5 fps Full Sensor, Bin by 10 (204 x 204) 21.6 fps 30.5 fps 30.0 fps 38.1 fps a. The specified performance is valid when using a computer with the specifications listed in the Recommended System Requirements table given below.
Chapter 7: CM401 Specifications Specification Value Maximum Stepping Velocity 4577 steps/s Positive Feedback Quadrature Encode (QEP) Input, 5 V Encoder Feedback Bandwidth 16 MHz Position Counter 32-Bit Operating Modes Position and Velocity Velocity Profile Trapezoid Motor Drive Connector Mechanical Specifications...
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Chapter 7: CM401 Specifications Specification Value Intensity Switch Time <3 s (10% to 90% Intensity) Shutter States Open, Closed Independent of Intensity Setting Shutter Switch Time 5 ms 1 Hz (Extended Duration) Shutter Repetition Rate (Max) 10 Hz (Burst) Bulb Module Life Time...
Waste Treatment is Your Own Responsibility If you do not return an “end of life” unit to Thorlabs, you must hand it to a company specialized in waste recovery. Do not dispose of the unit in a litter bin or at a public waste disposal site.
Chapter 10: CM401 Thorlabs Worldwide Contacts Chapter 10 Thorlabs Worldwide Contacts For technical support or sales inquiries, please visit us at www.thorlabs.com/contact for our most up-to- date contact information. USA, Canada, and South America UK and Ireland Thorlabs, Inc. Thorlabs Ltd.
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