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Systec VX 95 Series Standard Operating Procedure
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Systec VX 95 Series Standard Operating Procedure

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Standard Operating Procedure
Title: USE AND MAINTENANCE OF THE SYSTEC VX 95 AUTOCLAVES
Location:
Autoclave Room, H31, CBE
1. PURPOSE
To describe the procedure for the use and maintenance of the Systec VX 95 Series Autoclaves; No.
CBE045 (Don Whitley Serial No. 3099) and CBE044 ( Don Whitley Serial No. 3098) in the CBE
Autoclave Room (H31).
2. SCOPE
This SOP describes operational procedures for using the Systec VX95 Series (No CBE045/CBE044)
autoclaves for :
1)steam sterilization and safe disposal of solid biological waste that may contain biological agents
or Genetically Modified Organisms, as defined in the CBE Code of Practice, where solid waste may
include solid culture and stocks of infectious material, culture dishes, flasks and related devices,
sharps bins, contaminated solid items such as paper towels, plastic pipettes, pipette tips, vials and
gloves.
2) steam sterilization of aqueous solutions and media not exceeding 5L,
3)sterilization of reusable equipment (not waste) that may include bottles, beakers, pipettes, forceps;
tubing etc. All procedures described in this SOP for solid or liquid waste treatment have been
validated. Typical waste batches are described in Annexes at the end of the SOP.
SPECIAL NOTES: HEALTH & SAFETY
Risks to the operator
The autoclaves are state-of-the-art and built according to standard safety regulations. Nevertheless, the
danger of death or injury to the user or a third party, or damage to the autoclave or other material assets,
can arise when using the appliance. For this reason, the autoclave is only to be used as it is intended to
be used and in a faultless condition with regard to safety. NOTE: Faults that could impact on safety
must be resolved immediately. The following residual risks result from the function of the autoclave,
and must be considered at all times:
(i)
Crushing and or amputation hazards - There is a danger of crushing or amputation of hands or
fingers between the edge of the door and the edge of the container if closing of the door is no
longer damped by spring or hydraulics.
(ii)
Burn and scald hazards when unloading the autoclave (for some designated cycles) from (1)
hot vessels or liquid escape from vessels leaking (2) hot surfaces inside the container or near the
sterilisation substance. Hot surfaces of the door and chamber (3) escape of hot clouds of steam
Version 004
Written by: A. Chandra
CONTROLLED COPY
Centre for Biological Engineering
th
Effective Date: 28
June 2020
Reviewed by: P.Hourd
Page 1 of 26
SOP025
Review 28
Approved by: R.I.Temple
th
June 2022

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Summary of Contents for Systec VX 95 Series

  • Page 1 Autoclave Room, H31, CBE 1. PURPOSE To describe the procedure for the use and maintenance of the Systec VX 95 Series Autoclaves; No. CBE045 (Don Whitley Serial No. 3099) and CBE044 ( Don Whitley Serial No. 3098) in the CBE Autoclave Room (H31).
  • Page 2 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP025 Title: USE AND MAINTENANCE OF THE SYSTEC VX 95 AUTOCLAVES Location: Autoclave Room, H31, CBE on opening the door (4) unathorised or improper programing of parameters (5) unrecognised errors and defects or poor maintenance procedures.
  • Page 3 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP025 Title: USE AND MAINTENANCE OF THE SYSTEC VX 95 AUTOCLAVES Location: Autoclave Room, H31, CBE (iv) Materials contaminated with Virkon are NOT to be autoclaved. Any strong oxidizing material i.e. dry hypo chlorite’s, MUST NOT be autoclaved with organic materials such as paper, cloth or oil.
  • Page 4 It is the Laboratory Managers or designated Responsible Person duty to ensure that the Insurance inspection is performed with the Systec engineer present and the inspection certificate is lodged with Technical Resources Manager in the Wolfson School.

  • Page 5: Equipment And Materials

    CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP025 Title: USE AND MAINTENANCE OF THE SYSTEC VX 95 AUTOCLAVES Location: Autoclave Room, H31, CBE Principal investigators/supervisor’s MUST ensure that his/her employees are trained before (vi) Mastercopy operating any autoclave unit. All operators (regardless of experience) must have successfully completed an authorized training session on the safe operating procedures of autoclaves.
  • Page 6 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP025 Title: USE AND MAINTENANCE OF THE SYSTEC VX 95 AUTOCLAVES Location: Autoclave Room, H31, CBE 5.1 Opening the Autoclave Door Turn on the power for the autoclave (ii) CAUTION: Before opening the door check the log to see if the autoclave has recently performed a sterilisation cycle.
  • Page 7 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP025 Title: USE AND MAINTENANCE OF THE SYSTEC VX 95 AUTOCLAVES Location: Autoclave Room, H31, CBE ALL waste must be sterilised using validate cycles. Non validated cycles should only be used for sterilising equipment which will remain in the laboratory.
  • Page 8 (described in Annex 3). This is as per the validation Certificate provided by Don Whitley Scientific Limited. A copy is available in the Systec VX- 95 equipment folder. The load-probe must be placed in the blank bottle.
  • Page 9 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP025 Title: USE AND MAINTENANCE OF THE SYSTEC VX 95 AUTOCLAVES Location: Autoclave Room, H31, CBE (iii) Label the bags to identify the laboratory area from which it came (iv) Ensure that the solid form waste is free from excess liquids and sharp objects that could puncture the waste bag.
  • Page 10 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP025 Title: USE AND MAINTENANCE OF THE SYSTEC VX 95 AUTOCLAVES Location: Autoclave Room, H31, CBE (ii) Place the waste in CBE approved transparent autoclave bags. DO NOT use any other...
  • Page 11 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP025 Title: USE AND MAINTENANCE OF THE SYSTEC VX 95 AUTOCLAVES Location: Autoclave Room, H31, CBE (iii) Seal the sharps bin following the instructions on the side of the container (iv) Place sharps boxes in the steel bucket described in the Equipment and Materials List .
  • Page 12 CONTROLLED COPY Centre for Biological Engineering Mastercopy Standard Operating Procedure SOP025 Title: USE AND MAINTENANCE OF THE SYSTEC VX 95 AUTOCLAVES Location: Autoclave Room, H31, CBE (iii) Prepare a second container with distilled water (the Blank). This must equal the volume of the liquid to be sterilised to a maximum of 2L.
  • Page 13 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP025 Mastercopy Title: USE AND MAINTENANCE OF THE SYSTEC VX 95 AUTOCLAVES Location: Autoclave Room, H31, CBE (iii) Ensure that all solid items are suitable for steam sterilization at temperatures >121 °C or >134 °C and ensure that any packaging used is permeable to steam.
  • Page 14 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP025 Title: USE AND MAINTENANCE OF THE SYSTEC VX 95 AUTOCLAVES Location: Autoclave Room, H31, CBE Mastercopy Only 5.3.6.1. Cycle Selection use these cycles for liquids! WARNING: The sterilization of liquids in solids cycles is highly dangerous through boiling delays or exploding vessels and can result in serious injuries.
  • Page 15 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP025 Title: USE AND MAINTENANCE OF THE SYSTEC VX 95 AUTOCLAVES Location: Autoclave Room, H31, CBE reference vessel. The reference vessel should have the same size and fill volume as the vessel containing the liquid to be sterilized.
  • Page 16 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP025 Title: USE AND MAINTENANCE OF THE SYSTEC VX 95 AUTOCLAVES Location: Autoclave Room, H31, CBE (ix) Place the flexible temperature sensor in the liquid or in a reference vessel. The reference vessel should have the same size and fill volume as the vessel containing the liquid to be sterilized.
  • Page 17 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP025 Title: USE AND MAINTENANCE OF THE SYSTEC VX 95 AUTOCLAVES Location: Autoclave Room, H31, CBE out and used in accordance with the prescribed procedure, as described in the sections “Opening the door”...
  • Page 18 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP025 Title: USE AND MAINTENANCE OF THE SYSTEC VX 95 AUTOCLAVES Location: Autoclave Room, H31, CBE 5.7. Opening the door When the sterilization process is completely finished and the specified unloading temperature and atmospheric conditions have been reached, the message ‘Opening Door’...
  • Page 19 Centre for Biological Engineering Standard Operating Procedure SOP025 Title: USE AND MAINTENANCE OF THE SYSTEC VX 95 AUTOCLAVES Location: Autoclave Room, H31, CBE CAUTION: There is danger of crushing and or amputation between the door and the edge of the autoclave chamber if the door has not been completely opened.
  • Page 20 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP025 Title: USE AND MAINTENANCE OF THE SYSTEC VX 95 AUTOCLAVES Location: Autoclave Room, H31, CBE 5.10. Autoclave Load Testing and Recording Autoclave runs used for waste management must be checked on each occasion by the use of recording strips, data logging, etc.
  • Page 21 Location: Autoclave Room, H31, CBE The Systec 95 Series autoclave is used for decontamination of solid form waste loads containing hazardous or potentially hazardous biological agents or genetically modified organisms. Validation is required to demonstrate that the process is effective in achieving a 100% kill i.e. made safe.
  • Page 22 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP025 Title: USE AND MAINTENANCE OF THE SYSTEC VX 95 AUTOCLAVES Location: Autoclave Room, H31, CBE • Remove the baskets or other vessels from the autoclave. Clean the interior of the autoclave and the baskets with a mild cleaning agent and water.
  • Page 23 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP025 Title: USE AND MAINTENANCE OF THE SYSTEC VX 95 AUTOCLAVES Location: Autoclave Room, H31, CBE 5.13. Spill Response Spills may occur from a boil over or breakage of containers. All spills MUST be cleaned up immediately.
  • Page 24 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP025 Title: USE AND MAINTENANCE OF THE SYSTEC VX 95 AUTOCLAVES Location: Autoclave Room, H31, CBE (iv) External maintenance and servicing of the equipment can only be performed after it or...
  • Page 25 2 x small bottle • 1 pair of small gloves • Annex 3 . The load for validation of waste cycle No 6 (liquids) for the Autoclave by Systec included • 1 x 5L bottle with water • 2 x 2L bottles with water (one with probe) •...
  • Page 26 CONTROLLED COPY Centre for Biological Engineering Standard Operating Procedure SOP025 Title: USE AND MAINTENANCE OF THE SYSTEC VX 95 AUTOCLAVES Location: Autoclave Room, H31, CBE SOP History Version Date Revised/ Revision Summary Reviewed Reviewed Version Number 21 October 2009 1. Added the validation of two more cycles after validation on 1 October 2009 which are: 5 –...

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