FEI Tecnai G2 F20 Operating Procedures Manual

FEI Tecnai G

1. Startup

1. Sign-up in the microscope log-book
2. Startup the software if not already running: Start the software in this
sequence:
1. Tecnai User Interface
2. Digital Micrograph
3. TIA (TEM Imaging and Analysis)
3. Check the vacuum: Look at the
GUN, COLUMN and CAMERA values should look like the ones below and
highlighted in green. Gun will always be 1 Log.
4. Fill the cold-trap dewar with LN
until it is full. If you are the first user of the day, refill it again after 30 minutes.
Refill the LN
5. Inserting the specimen holder
Room temperature sample
There are two things to check prior to inserting the specimen holder:
(a) Under the Search
Control then Holder. This will reset X, Y, Z, and α on the stage and avoid damaging the
2
F20 Operating Procedures
(6/01/2017)
Vacuum
2
at least every 3 hrs to keep it full
2
2
tab, in the Stage menu, click on the flap-out menu and click
panel under the
: Cover the screen window first. Fill the dewar
Setup menu. The