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Dynamica HALO DNAmaster Operation Manual
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Dynamica HALO DNAmaster Operation Manual

Bio-spectrophotometer
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HALO DNAmaster
Bio-spectrophotometer
Operation Manual
Model HALO DNAmaster Bio-spectrophotometer
Revision : D01-201203

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Summary of Contents for Dynamica HALO DNAmaster

  • Page 1 HALO DNAmaster Bio-spectrophotometer Operation Manual Model HALO DNAmaster Bio-spectrophotometer Revision : D01-201203...
  • Page 2 IMPORTANT Precautions on Electromagnetic Wave Interference 1. Possible Electromagnetic Wave Interference Caused by This Instrument When this instrument is used in a residential area or an adjacent area thereto, it may cause interference to radio and television reception. To prevent this, use the specified system connection cables in strict accordance with the instruction manual.
  • Page 3 • Confirm that any other device connected with the instrument is not affected by electromagnetic wave interference. Warranty on Product The Model HALO DNAmaster Series Spectrophotometer is warranted to operate according to the specifications given in the instruction manual, provided it is used in accordance with the instructions described in the manual.
  • Page 4 Limitations and Exclusions on Warranty Note that this warranty is void in the following cases, even if they occur within the warranty period. (a) Failure due to operation at a place not meeting the installation requirements specified by us (b) Failure due to power supply voltage/frequency other than specified by us or due to abnormality in power supply (c) Corrosion or deterioration of the tubing due to impurities contained in reagent, gas, air or cooling water supplied by the...
  • Page 5 Technical Service Installation of this instrument shall be carried out by or under supervision of qualified service personnel of Dynamica or its authorized service agent. Before installation of the instrument, the user shall make preparations for satisfying the installation requirements in accordance with the instruction manual.
  • Page 6 This manual is copyrighted by Dynamica with all rights reserved. No part of this manual may be reproduced or transmitted in any form or by any means without our express written permission.

  • Page 7: Safety Summary

    SAFETY SUMMARY For your safety please read the following precautions carefully before using the DNAmaster Spectrophotometer. General Safety Guidelines For safe handling of this product, please follow the instruction procedure in the manual for this product. Pay special attention to follow all the hazard warnings on the product and in the manual.
  • Page 8 SAFETY SUMMARY (Continued) General Safety Guidelines (Continued) In addition, the following “Attention” and “Note” are not directly related to the safety of a person: Attention: It is used to present warnings, which are not directly related to personal injury hazards. It is used to indicate prevention...

  • Page 9: Before Using

    SAFETY SUMMARY (Continued) General Safety Guidelines (Continued) Before Using Before using this product, please make sure you read and understand the instructions. Please keep this manual in a safe and easily accessible place so that you can use it when necessary.
  • Page 10 (delivery), please notify your local Dynamica sales representative or nearest Dynamica service office. Technical support service for this instrument is available from s service agent approved or authorized by Dynamica within regular working hours or workdays. Please do not perform any other operations that are not included in the manual.
  • Page 11 SAFETY SUMMARY (Continued) WARNING: Poisoning from Organic Solvent Gas Handling Organic Solvents The organic solvent vapor may be harmful to your health. WARNING: Eye Injury from Organic Solvents Handling Organic Solvents Please wear protective glasses when using organic solvents. If the organic solvent should get into your eye, flush your eye immediately under running water for at least 5 minutes while keeping your eyelids open.
  • Page 12 SAFETY SUMMARY (Continued) WARNING: Electrical Shocks from Improper Grounding Electrical Shock from Improper Grounding When wiring the personal computer, power supply for the thermostatic cell holder and the like, please make sure to use the 3-prong wire (with ground) provided. WARNING: Electrical Shock Electrical Shock from Contact High voltage is used inside of the equipment.
  • Page 13 SAFETY SUMMARY (Continued) CAUTION: Injury from incidental contact with the unit. Handling the sample compartment cover When you open and close the sample compartment cover, please be careful not to get your hand or hair caught. Please assure your safety before operation. CAUTION: Fatigue due to Prolonged Work Data Processing Viewing the display in your work can cause eye and physical fatigue if you continue...
  • Page 14 SAFETY SUMMARY (Continued) NOTE Precautions regarding Accuracy and Precision of Measurements Do a routine check prior to taking measurements. In order to obtain accurate measuring results, please perform tests as instructed, following the instructions in Chapter 3. SAFETY-8...

  • Page 15: Fire Extinguishers

    Electricity The voltage for the Spectrophotometer system and personal computer must be a single-phase AC 100V to 200V; Variations in the voltage and noise generated in the power line will cause adverse effects on the spectrophotometer and may also cause accidents.

  • Page 16: Installation Environment

    Installation conditions Power requirements Power supply voltage: AC100, 115, 220, 230 or 240V Allowable fluctuation is within ±10% of rated voltage. Frequency: 50 or 60 Hz Allowable fluctuation is within ±4% of rated frequency. Power capacity: 200VA or more. A capacity of more than 500VA may be required for use with other accessory devices.
  • Page 17 DNAmaster Quick Manual The Instrument Touch screen display Take on/off button Measurement On/Off button Blue backlight- Standby mode Green backlight- Operating Read button The Read button on the unit can use as an alternative way to take the data during measurement. Touch screen DNAmaster’s LCD display is pressure sensing which can be used even with gloves on.

  • Page 18: Specification

    DNAmaster Quick Manual Specification DNAmaster Specifications Long life Xenon Flash Lamp source Lamp Detector Device Wavelength range: 200-900nm Measuring range: 0-4.0 OD Wavelength accuracy: +/-1nm Slit width: Noise: ~0.005 OD (RMS) Drift: ~0.005 OD Photometric Accuracy: +/-0.01 OD Photometric Repeatability: +/-0.005 OD Stray light: 0.5%T...

  • Page 19: The Display Screen

    DNAmaster Quick Manual The Display Screen Main menu Touch the icon for go to next screen DNA/RNA: Nuclei acid analysis DNA/RNA DYE: Dye incorporation PROTEIN: Protein determination PROTEIN UV: Protein UV & UV dye method OD600: Bacterial cell culture measurement SPECTRO: Traditional spectrophotometer function FILE: Saved method and data...
  • Page 20 DNAmaster Quick Manual DNA/RNA measurement DNA/RNA Select measurement from dsDNA, ssDNA, RNA, Oligonucleotides Press SET for detail setting LOAD for reload saved parameter ZERO for blank or reference sample before measurement READ for measurement PRN for result printing Select between Press ZERO key after new setting, this Note: different sample...
  • Page 21 DNAmaster Quick Manual Dye incorporation Select dye incorporation method from dsDNA, ssDNA, RNA, Oligonucleotides Press 1. SET for detail setting 2. LOAD for reload saved parameter 3. ZERO for blank before measurement 4. READ for measurement 5. PRN for result printing Select between different sample number...
  • Page 22 DNAmaster Quick Manual Dye type selection For selected preset/ custom dye type Input 1. Dye Type For custom dye Input 1. Dye Abs Max for maximum absorbance wavelength of the custom dye 2. Dye Ext Coefficient for dye-dependent extinction coefficient 3.
  • Page 23 DNAmaster Quick Manual Protein measurement PROTEIN Select measurement from Bradford, Lowry, BCA and Biuret Standard curve setup Press SET for detail setting LOAD for reload saved parameter ZERO for blank or reference sample before measurement READ for measurement PRN for result printing Press ZERO key after new setting, Note: this will be used for all subsequent...
  • Page 24 DNAmaster Quick Manual For standard concentration setting Input the concentration of standard used Press OK to next step or Back to previous screen For standard measurement For Standard curve setup Press ZERO for blank before measurement READ for measurement PRN for result printing Press ZERO key after new setting, Note: this will be used for all subsequent...
  • Page 25 DNAmaster Quick Manual For K-factor setting Input the K-factor Press OK to next step or Back to return to previous screen PROTEIN SAMPLE MEASUREMENT Press ZERO for blank or reference sample before measurement READ for measurement PRN for result printing Press ZERO key after new setting, this Note: will be used for all subsequent samples...
  • Page 26 DNAmaster Quick Manual PROTEIN UV Select measurement from Protein UV and Protein UV Dye Press SET for detail setting LOAD for reload saved parameter ZERO for blank or reference sample before measurement READ for measurement PRN for result printing Select between Press ZERO key after new Note: different sample...
  • Page 27 DNAmaster Quick Manual PROTEIN UV DYE Select measurement from Protein UV and Protein UV Dye Press SET for detail setting LOAD for reload saved parameter ZERO for blank or reference sample before measurement READ for measurement PRN for result printing Select between Press ZERO key after new setting, Note:...
  • Page 28 DNAmaster Quick Manual Dye type selection Input PRO EXT COEFFICIENT DYE TYPE for dye selection Press OK to next step or BACK to return to previous screen Custom dye type selection For selected custom dye type Input 1. PRO EXT COEFFICIENT 2.
  • Page 29 DNAmaster Quick Manual Analysis of Bacterial Growth OD600 Press 1. SET for detail setting 2. LOAD for reload saved parameter 3. ZERO for blank or reference sample before measurement 4. READ for measurement 5. PRN for result printing Press ZERO key after new setting, this Note: will be used for all subsequent samples Select between...
  • Page 30 DNAmaster Quick Manual Spectrophotometer function Photometry Press SET for detail setting LOAD for reload saved parameter ZERO for blank or reference sample before measurement READ for measurement PRN for result printing Press ZERO key after new setting, this Note: will be used for all subsequent samples Select between different sample number...
  • Page 31 DNAmaster Quick Manual Wavelength scan Press 1. SET for detail setting 2. LOAD for reload saved parameter 3. ZERO for blank or reference sample before measurement 4. READ for measurement 5. PRN for result printing 540nm Press ZERO key after new setting, this Note: will be used for all subsequent samples Parameter setting...
  • Page 32 DNAmaster Quick Manual Wavelength scan Press ZERO for blank or reference sample before measurement READ for measurement PRN for result printing Press ZERO key after new setting, this Note: will be used for all subsequent samples Data Process 1. P-V- Peak- Valley function display the peak and valley 2.
  • Page 33 DNAmaster Quick Manual Kinetics Press 1. SET for detail setting 2. LOAD for reload saved parameter 3. ZERO for blank or reference sample before measurement 4. READ for measurement 5. PRN for result printing Press ZERO key after new setting, this Note: will be used for all subsequent samples Parameter setting...
  • Page 34 DNAmaster Quick Manual Kinetics Press 1. ZERO for blank or reference sample before measurement 2. READ for measurement 3. PRN for result printing Press ZERO key after new setting, this Note: will be used for all subsequent samples Data Process 5.
  • Page 35 DNAmaster Quick Manual Concentration Press 1. SET for detail setting 2. LOAD for reload saved parameter 3. ZERO for blank or reference sample before measurement 4. READ for measurement 5. PRN for result printing Press ZERO key after new setting, this Note: will be used for all subsequent samples Parameter setting...
  • Page 36 DNAmaster Quick Manual For standard concentration setting Input the concentration of standard used Press OK to next step or Back to previous screen Concentration measurement After setting calibration curve Press 1. ZERO for blank or reference sample before measurement 2. READ for measurement 3.
  • Page 37 DNAmaster Quick Manual File Data (IN/SD) 1. Select between data stored in internal memory or SD card 2. Open/Delete the method or data stored Page 21 Version 1.0...
  • Page 38 DNAmaster Quick Manual System setting System Home screen- Select the function show on startup Memory Storage- Select storage by SD-card or internal memory Peak Threshold- Select the threshold for automatic peak picking function Factory reset- reset back to factory default setting Time Time and Date setting Page 22...
  • Page 39 DNAmaster Quick Manual GLP/GMP Perform checking self diagnostic function Additional service tools may required (please contact your local distributor for further assistance) User 1. User Name input 2. Company name input 3. Display contrast of the screen 4. Energy saving mode for screen turn off after 10 minutes without using 5.
  • Page 40 DNAmaster Quick Manual Appendix 1 How to use ultramicro cell The ultramicro cell is a 10mm x 10mm cuvette that fits into the DNAmaster. A 8.5 mm beam height was setup for DNAmaster. The cell has a system of fiber optics and prisms that will reflect the light beam up to the sampling window and then back to the detector on the instrument.
  • Page 41 DNAmaster Quick Manual Quantification of Nucleic Acids For the different types of nucleic acid solutions the average dynamic range of the absorbance relating to the concentration (ng/μl) results and its required sample volume as follows (depending on the lightpath): Factor 2 mm Lid 1 mm Lid 0.2 mm Lid...
  • Page 42 DNAmaster Quick Manual Appendix 2 Temperature control model The below instruction only applicable for temperature control model. Ultramicro cell is not applicable for temperature control function. TM function For analyzing DNA melting temperature at 260nm, select the OD600/TM function in the main menu. Press 1.
  • Page 43 DNAmaster Quick Manual Parameter setting Input Pathlength Sample name Y-MAX/ Y-MIN for Y-axis limit can be shown in the graph HEAT MODE for different heating profile, selection from 1℃/min, 2℃ /min, Fast heating to 50deg.C then 1 ℃/min or Fast heating to 50deg.C then 2℃/min Starting temperature (minimum 20 ℃)
  • Page 44 P: +41 44 744 2828 F: +41 44 744 2864 Email: info@dynamica-eu.com Web: www.dynamica-eu.com Australasia Dynamica Pty Ltd Unit C3 Hallmarc Business Park Corner Centre and Westall Roads Clayton, Victoria, 3168, Australia P: +61 3 8540 5988 F: +61 3 8540 5981 Email: info@dynamica.com,au...