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Dynamica HALO XB-10/BIOmaster/VIS-20/ENVmaster Instruction Manual

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Accessories for Model HALO XB-10/VIS-
20/BIOmaster UV-VIS Spectrophotometer
HALO XB-10/VIS-20
/BIOmaster
Instruction manual
Revision : D01-201407

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Summary of Contents for Dynamica HALO XB-10/BIOmaster/VIS-20/ENVmaster

  • Page 1 HALO XB-10/VIS-20 /BIOmaster Instruction manual Accessories for Model HALO XB-10/VIS- 20/BIOmaster UV-VIS Spectrophotometer Revision : D01-201407...
  • Page 2 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual XB-10/BIOmaster/VIS-20/ENVmaster Spectrophotometer Thank you for your purchase of Dynamica XB-10/BIOmaster/VIS-20/ENVmaster spectrophotometer. In order to ensure proper use and your safety, please read this manual carefully and keep it well before using the instrument. Information contained in this manual is subject to change without notice for product appearance and...
  • Page 3 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual IMPORTANT Precautions on Electromagnetic Wave Interference （1）Possible Electromagnetic Wave Interference Caused by This Instrument When this instrument is used in a residential area or an adjacent area thereto, it may cause interference to radio and television reception. To prevent this, use the specified system connection cables in strict accordance with the instruction manual.
  • Page 4 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual possible sources of electromagnetic wave interference are connected.  Confirm that any other device connected with the instrument is not affected by electromagnetic wave interference. Warranty on Product Model XB-10/BIOmaster/VIS-20/ENVmaster spectrophotometer warranted to operate according to the specifications given in the instruction manual, provided it is used in accordance with the instructions described in the manual.
  • Page 5 Before installation of the instrument, the user shall make preparations for satisfying the installation requirements in accordance with the instruction manual. If relocation of the instrument becomes necessary after initial installation (delivery), please notify your local sales representative or nearest service office of Dynamica.
  • Page 6 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual Disposal this instrument When you discard equipment, please check and discard a related statute etc. or ask the service section of Dynamica. Other Precautions （1）Handling of Chemicals and Samples (a)The user is responsible for following relevant legal standards and regulations in handling, storage and discarding of chemicals and samples used in analytical operations of this instrument.
  • Page 7 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual SAFETY SUMMARY CAUTION For your safety please read the following precautions carefully before using the instrument. General Safety Guidelines  For safe handling of this product, please follow the instruction procedure in the manual for this product. ...
  • Page 8 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual △ Attention: It is used to present warnings, which are not directly related to personal injury hazards. It is used to indicate prevention against damage to the equipment. ◇ Note: This is used to indicate instructions that enable you to operate the equipment accurately and perform accurate measurements.
  • Page 9 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual SAFETY SUMMARY (Continued) General Safety Guidelines (Continued) Before using  Before using this product, please make sure you read and understand the instructions.  Please keep this manual in a safe and easily accessible place so that you can use it when necessary.
  • Page 10 Dynamica within regular working hours or workdays. Please do not perform any other operations that are not included in the ■ manual. If any problem occurs with the equipment, please contact the agent from whom you purchased it or the service department of Dynamica.
  • Page 11 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual SAFETY SUMMARY (Continued) WARNING: Poisoning from Organic Solvent Gas Handling Organic Solvents The organic solvent vapor may be harmful to your health. ■ WARNING: Eye Injury from Organic Solvents Handling Organic Solvents Please wear protective glasses when using organic solvents. If the ■...
  • Page 12 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual SAFETY SUMMARY (Continued) WARNING: Electrical Shocks from Improper Grounding When wiring the personal computer, power supply for the thermostatic cell ■ holder and the like, please make sure to use the 3-prong wire (with ground) provided. WARNING：Electrical Shock from Contact High voltage is used inside of the equipment, be sure to turn off the unit ■...
  • Page 13 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual SAFETY SUMMARY (Continued) Electricity (a) The voltage for the Spectrophotometer system and personal computer must be a single-phase AC 100V to 240V; Variations in the voltage and noise generated in the power line will cause adverse effects on the spectrophotometer and may also cause accidents.
  • Page 14 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual Functional characteristics This series instrument includes 4 model number spectrophotometers. Model VIS-20 is traditonal visible spectrophotometer, ENVmaster is enhancement type spectrophotometer with method invocation mode, XB-10 is traditonal UV-VIS spectrophotometer, BIOmaster is UV-VIS spectrophotometer only for nucleic acid and protein detection.
  • Page 15 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual Appearance Keypad Though the instrument uses touch display screen, all operation will be acheived by touch screen. The purpose of setting the two shortcut key is to make easy operation. BACK. Return to the previous menu. MEASURE. Begin to a new measurement.
  • Page 16 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual Operation environment Power Voltage: AC 100V～240V, Frequency :50/60Hz±1Hz, Capacity: more than 200W Grounding line resistance of 10Ω or less is required The input power mutations, without interference from other large electrical equipment Operating 5~35 Deg C temperature In order to perform a measurement under the most stable condition, we recommend that the instrument is used in an air conditioned room of 20 ~ 25 Deg C.
  • Page 17 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual Specification（I） VIS-20/ENVmaster Wavelength range 320～1100nm Wavelength controlled 0.2nm variable Wavelength accuracy ±1nm Wavelength repeatability ≤0.5nm Transmittance accuracy ±0.5％T（NIST 930 Filter） Transmittance repeatability 0.2％T Baseline flatness ±0.002Abs（330～1090nm） Noise level ≤0.0008Abs（500nm） Baseline stability ≤0.0008Abs/h（500nm, 2 hours after power on） Spectral bandwidth 6nm±1.2nm Stray light...
  • Page 18 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual Specification（II） XB-10/BIOmaster Wavelength range 190～1000nm Wavelength controlled 0.2nm variable Wavelength accuracy ±2nm Wavelength repeatability ≤1nm Transmittance accuracy ±1％T（NIST 930 Filter） Transmittance repeatability 0.5％T Baseline flatness ±0.005Abs（200～990nm） Noise level ≤0.005Abs（250nm 处） Baseline stability ≤0.005Abs/h（250nm, 2 hours after light on） Spectral bandwidth 5nm±1nm Stray light...
  • Page 19 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual The operation interface Function shortcut button These buttons appear on every interface and can quickly switch to different measurement functions. Measure button These buttons appear on every measure interface. Measure basic operation. Processing button These buttons appear on scan interface. For the results of the scan data processing and according to different scaning characteristic, automatically varycorresponding processing ways.
  • Page 20 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual Word input keypad This keypad appears at the area where can input word, for example, sample name, user and document name. BACK close word input keypad CLEAR clear incorrect word Word switchover majuscule, lowercase, digit and sign Enter ensure input word Digit input keypad...
  • Page 21 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual Main interface Click icon into the corresponding measure interface ABS/%T wavelength photometric WL SCAN wavelength scanning TIME SCAN time scanning CONC concentration regression MANAGER measure method manger including data-saving（except VIS-20） SETTING parameter setting...
  • Page 22 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual Large character measure This interface is used to measure single wavelength’s absorbance or transmission. In any interface （non-execution of measure in the interface）, click value in the status bar into the large character measure. %T button absorbance or transmission mode ZERO button zero absorbance（blank sample）...
  • Page 23 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual ABS/%T given wavelength photometric measure The interface is used to measure specified wavelength’s absorbance or transmission. The wavelength value is less than 6. Each measure document includes 200 (maximum) measure results. Measure buttons READ begin to measure parameter setting ZERO zero absorbance（blank sample）...
  • Page 24 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual ABS/%T given wavelength photometric parameter setting DATA MODE mode setting, including %T or ABS PATHLENGTH cuvette optical length, it can print, but cannot regulate absorbance value SAMPLE NAME sample name, less than 8 characters WL NUMBER wavelength quantity, less than 6 WL1~WL6 wavelength, wavelength value allow not sort finish parameter setting, back to measure interface automatically...
  • Page 25 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual Wavelength Scan The interface is used to do wavelength scan measure of sample. It can scan the sample transmission, absorbance and the change between energy and wavelength (the purpose of energy scan is to judge the instrument current status）. Functions: the peak value judge, smooth and constant computing, peak area calculation, spectrogram data point cursor tracking, spectrogram automatic and manual zoom of the scan results.
  • Page 26 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual Wavelength scan parameter setting DATA MODE mode setting, including %T or ABS SAMPLE NAME sample name, less than 8 characters PEAK THRESHOL sentenced peak threshold setting, the longitudinal axis is greater than the threshold value, the peak is displayed START WL(nm) scan initiative wavelength END WL(nm)
  • Page 27 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual Wavelength scan results and data processing Data processing buttons peak value list button, according to the parameter setting threshold, show peak value larger than threshold Smooth Smooth function. Smooth the spectrogram, less than 6 smoothness Calcu Calculation function. Addition, subtraction, multiplication and division Area Peak area calculation function.
  • Page 28 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual Time scan This interface is used to do time scan measure of sample under specified wavelength. It can scan the sample transmission, absorbance and the time. Functions: dynamics calculation, smooth and constant computing, peak area calculation, spectrogram data point cursor tracking, spectrogram automatic and manual zoom of the scan results.
  • Page 29 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual TIME scan parameter setting DATA MODE mode setting, including %T or ABS SAMPLE NAME sample name, less than 8 characters WL(nm) scanning wavelength SCAN TIME(s) scan time. (unit: s). more than 60s, max: 60000s INTERVAL(s) scan interval. AUTO, 0.5s, 1s, 2s, 5s, 10s, 100s. AUTO interval, when scan time is less than 1000s, scan interval is 1s.
  • Page 30 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual TIME scan results and data processing Data processing buttons Kinetics Dynamics calculation function. According to setting time range, make dynamics calculation Smooth Smooth function. Make of spectrogram smooth processing Calcu Calculation function. Addition, subtraction, multiplication and division Area Peak area calculation function.
  • Page 31 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual CONC concentration regression and measure This interface is used to do concentration measure of specified wavelength. The specified wavelength quantity is less than 3. The concentration regression way is first-order linear regression. The sample quantity is less than 9. The times of standard sample average is less than 5. Regression spectrogram and mathematical equation is displayed.
  • Page 32 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual CONC concentration regression and parameter setting ( I ) SAMPLE NAME Sample name, less than 8 characters WL NUMBER (nm) Measure wavelength number, less than 3 WL1~ WL3 (nm) Wavelength, wavelength value allow not sort UNIT Standard concentration CALIBRATION Concentration calculation method.
  • Page 33 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual CONC concentration regression and parameter setting（II） If using standard sample regression calculation method（CALIBRATION , select STD）, it will show this parameter setting interface. STD1~STD9: 9 standard sample concentration, concentration must be sorted (in general, sort ascending). If using regression equation parameters to enter calculation method（CALIBRATION, select K-Factory）,it will show this parameter setting interface.
  • Page 34 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual MANAGER interface（except VIS-20） This interface is used to manage sample measure methods. User can select and save measure methods. Preview and open previous measure methods. Use this function, user must load SD card. Measure methods and measure results document are saved in SD card. METHOD measure methods manger ABS/%T...
  • Page 35 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual Method document manager interface（except VIS-20） According to the selected method of the prior interface（method manager interface）, open method document. Each page will appear 6 method documents. Using this function, the user must load SD card. Method documents are saved in SD card. Method document display saved method document Preview...
  • Page 36 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual Data document manager interface This interface is used to mange all method data document. Open or delete the measure data document . Each page displays 10 data document. Using this function, the user must load SD card. Measure data is saved in SD card.
  • Page 37 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual System setting interface This interface is used to set up the system of the instrument. SYSTEM Enter into system parameter setting TIME Enter into time setting GLP/GMP Enter into performance self-check USER NAME Input operator name. Less than 20 characters COMPANY Input company name.
  • Page 38 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual Time setting interface This interface is used to set up time. The instrument has power-down protection capacity (in general, about 96 hours). Switching off the instrument for 96 hours, the user need to reset time, but the setting data will be not lost.
  • Page 39 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual SETTING GLP/GMP performance self-check interface This interface is used to check instrument’s performance. Finishing test, it will display measure result automatically, and judge results. It meets to routine application standards. NOISE noise level test STABILITY stability test, preheating for 2 hours BASELINE FLATNESS baseline flatness test HARDWARE...
  • Page 40 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual BIOmaster interface BIOmaster is only for analysis of nucleic acids and proteins. Besides the special funciton measure interface, it also own measure funcitions of traditonal spectrophotometer. BIOmaster mian interface Click the icon, enter into corresponding measure interface. DNA/RNA nucleic acids analysis PROTEIN...
  • Page 41 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual BIOmaster nucleic acids analysis interface There are 4 methods in the DNA/RNA nucleic acids analysis interface, dsDNA, ssDNA, RNA, OLIGO. Click button to select measure method. Measure buttons READ being to measure parameter setting ZERO zero absorbance ( blank sample) at all setting wavelength point LOAD load original measure results or measure method document print data...
  • Page 42 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual BIOmaster nucleic acids parameter setting interface FACTOR regulate parameters BACKGROUND background calibration PATHLENGTH optical length of cuvette（optical length is less than 10mm） DI-FACTOR dilution factor UNIT concentration units SAMPLE NAME sample name Finish parameter setting, back to measure interface automatically Save save parameter (SD card)
  • Page 43 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual BIOmaster protein analysis interface There are 5 analysis methods in protein determination interface, PROTEINUV, BRADFORD, BSA, LOWRY, BIURET. Click button to select measure methods. PROTEINUV functions Measure buttons READ begin to measure parameter setting ZERO zero absorbance ( blank sample) at all setting wavelength point LOAD load original measure results or measure method document print data...
  • Page 44 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual BIOmaster protein analysis interface PROTEINUV parameter setting interface A280 FACTOR correction factor at 280nm position A260 FACTOR correction factor at 260nm position PATHLENGTH optical length of cuvette（optical length is less than 10mm） BACKGROUND background calibration PROTEIN select measure methods DI-FACTOR dilution factor UNIT...
  • Page 45 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual BIOmaster protein analysis interface select parametre of MOLAR EXT C (self-defining) protein measure method in PROTEINUV parameter setting interface WL(nm） Wavelength value MOLAR EXT C Molar extinction conefficient correction factor MOLECULAR W Molecular weight molecular weight. According to the two correction factors, calculate correction factor at A280 FACTOR automatically Finish parameter setting, back to measure interface automatically Back...
  • Page 46 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual BIOmaster protein analysis interface select parametre of EXT COEFF (self-defining) protein measure method in PROTEINUV parameter setting interface WL(nm) Wavelength value EXT COEFF Extinction conefficient correction factor. According to the correction factors, calculate correction factor at A280 FACTOR automatically Finish parameter setting, back to measure interface automatically Back Back to the prior parameter setting interface...
  • Page 47 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual BIOmaster protein analysis interface BRADFORD measure interface Measure buttons READ begin to measure parameter setting ZERO zero absorbance ( blank sample) at all setting wavelength point LOAD load original measure results or measure method document print data...
  • Page 48 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual BIOmaster protein analysis interface BRADFORD parameter setting interface WL(nm) Wavelength value PATHLENGTH optical length of cuvette（optical length is less than 10mm） CALIBRATION concentration regression way STD NUMBER concentration standard sample number REPLICATES measure times DI-FACTOR: dilution factor UNIT concentration units SAMPLE NAME...
  • Page 49 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual BIOmaster protein analysis interface CALIBRATION（concentraton）standard sample setting interface in BRADFORD parameter setting interface STD1~3 standard sample concentration setting （According to the number of concentration in the upper layer interface, it will display corresponding setting） Finish parameter setting, back to measure interface automatically Save save parameter (SD card)
  • Page 50 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual BIOmaster bacterial cell culture measurement interface OD600 measure interface Measure buttons READ begin to measure parameter setting ZERO zero absorbance (blank sample) at all setting wavelength point LOAD load original measure results or measure method document print data...
  • Page 51 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual BIOmaster bacterial cell culture measurement interface OD600 parameter setting interface（I） WL(nm) Test wavelength PATHLENGTH optical length of cuvette (optical length is less than 10mm) UNIT concentration units SAMPLE NAME sample name Finish parameter setting, back to measure interface automatically Save save parameter (SD card)
  • Page 52 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual BIOmaster bacterial cell culture measurement interface OD600 parameter settinginterface（II） When select UNIT cells/ml, it will display above parameter setting interface WL(nm) Test wavelength PATHLENGTH Optical length of cuvette (optical length is less than 10mm) UNIT Concentration units SAMPLE NAME Sample name FACTOR Correction factor...
  • Page 53 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual BIOmaster spectrum interface ABS/%T given wavelength photometry direct-reading measure interface Wavelegnth scan interface...
  • Page 54 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual BIOmaster spectrum interface KINETICS dynamics measure interface CONC concentration regression and mesure interface...
  • Page 55 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual Accessories interface The instrument can automatically recognize three accessories with the automatic control functions. Auto sample sipper, auto 5-cell holder, electronic thermostat TC holder（only for XB-10/BIOmaster）. Remark: When installing accessories, the power of the instrument must keep off. Install and recognize auto 5-cuvette holder interface Click , display 5-cell holder setting interface.
  • Page 56 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual Install and recognize auto sample sipper interface Click , display auto sample sipper setting interface. SIP TIME (s) sample sipper time（UNIT: second） DELAY TIME (s) delay time between sample sipper and measure PURGE TIME (s) suck air after finishing measure, This feature is primarily used to separate the two samples in the pipeline.
  • Page 57 XB-10/BIOmaster/VIS-20/ENVmaster instruction manual Install and recognize electronic thermostat TC holder interface (only for XB-10/BIOmaster) Click , display temperature setting interface. TEMPERATURE (℃ ) setting control temperature TEMP CONTROL turn on or off temperature control function...
  • Page 58 The HALO Range UV-VIS Spectrophotometers Livingston Dynamica Scientific Limited Kirkton Campus, 4 Bain Square, Livingston, United Kingdom, EH54 7DQ P: +44(0)1908 211 900 F: +44(0)1908 211 909 Email: info@dynamica-eu.com Web: www.dynamica-eu.com Australasia Dynamica Pty Ltd ABN 16 126 043 052...

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