GE AKTA Valve INV-907 Instructions Manual page 13

Valve INV-907 Instructions 56-8101-01 Edition AE
Two techniques can be used for filling the sample loop; partial or complete
filling.
Type of filling
Partial filling
Complete filling
Partial Filling
Partial filling is used when high recovery is required. Partial filling allows
the injected volume to be changed without changing the loop and does not
waste sample.
Partial filling is achieved as follows:
1 Set injection valve to position LOAD.
2 Load the syringe with a large volume of buffer (5× the loop volume).
3 Fill the sample loop carefully with buffer.
4 Set the injection valve to position INJECT before taking out the syringe.
Note: If the syringe is taken out when the injection valve is in position
LOAD, self drainage will occur and air will enter the sample loop.
5 Load the syringe with the required volume of sample. No more than half
(50%) a loop volume of sample should be loaded into the loop.
6 Insert the syringe in position 3 on the injection valve. Set the injection
valve to position LOAD.
Note: Do not load the sample before the valve is in position LOAD.
7 Gently load the syringe contents into the loop.
8 Leave the syringe in position. The sample will be injected onto the
column when the valve is switched to INJECT in the method.
Volume to load
max 50% of the sample loop volume
2–5 times the sample loop volume
Operation 3
13