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Related Manuals for SPEX SamplePrep 6875D
Summary of Contents for SPEX SamplePrep 6875D
- Page 1 65 Liberty St., Metuchen, NJ 08840 1-855-GET-SPEX (438-7739) or 732-623-0465 www.spexsampleprep.com ® 6875D LARGE FREEZER/MILL OPERATING MANUAL For 6875D-115 and 6875D-230 Freezer/Mills this manual is Part Number 87052 1300-87052-1A, REV 1...
- Page 2 1976 Copyright Act or in writing from the publisher. Requests for permission should be addressed in writing to SPEX SamplePrep LLC, 65 Liberty Street, Metuchen, NJ 08840.
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Page 3: Table Of Contents
6875D Freezer/Mill TABLE OF CONTENTS SECTION DESCRIPTION PAGE Introduction ........................4 Specifications ........................5 Unpacking ........................... 6 Setting Up ..........................7 Electrical Connection ......................7 Power Switch and Controller .................... 8 Liquid Nitrogen Connection………………………………………………………………………………………….9 Lid, Coil Assembly and Gate, LN Sensor ................10 Grinding Vial Sizes, Adapters, and Vial Openers ............ -
Page 4: Introduction
NOTE: Do not operate the 6875D Freezer/Mill until you have read the Operating Manual and are familiar with the controls and operation. OPERATING CONDITIONS: Do not set up the 6875D Freezer/Mill in an insulated or confined space. -
Page 5: Specifications
6875D Freezer/Mill 2.0 6875D FREEZER/MILL SPECIFICATIONS Type of Mill: Dual Chamber Cryogenic impact mill Controller: Touchscreen Data Transfer: USB Port (back of controller) Grinding Mechanism: Steel impactor driven by magnetic coil Coolant: Liquid nitrogen (LN) Dimensions: 20 in. (50.8 cm) x 21½ in. (53.34 cm) x 25½ in. (64.77 cm) Weight: (empty, without vial or coolant) approx. -
Page 6: Unpacking
An Accessory Package is available to match your choice of vials for each new 6875D Freezer/Mill. The 6870L for large vials, the 6870M for mid-size vials, the 6870S for small vials or 6757 for microvials. The 6870S also includes a vial holder for running up to four small vials per 6875D grinding chamber. -
Page 7: Setting Up
Figure 2 – 6875D Freezer/Mill, Rear View 4.0 SETTING UP The 6875D Freezer/Mill weighs 78 pounds (35.38 Kg) empty. The lid opens from the front with the lid latch in the center. The AC Input module and the ON/OFF switch (which also serves as a circuit breaker) are on the back of the cabinet. -
Page 8: Power Switch And Controller
The viewing angle of the controller on the 6875D Freezer/Mill can be adjusted to minimize glare. To adjust the viewing angle, simply grasp the back of the controller box and gently pull it forward until the desired angle is reached, as shown in Figure 3. -
Page 9: Liquid Nitrogen Connection
(Figure 4). Then attach the LN transfer hose to the safety valve, and the other end to the inlet on the 6875D Freezer/Mill. The safety valve should be higher than the LN inlet on the Freezer/Mill. The purpose of the safety valve is to provide an exit for liquid nitrogen vapor in case the mill and LN Tank valves are both closed, and pressure builds up inside the hose. -
Page 10: Lid, Coil Assembly And Gate, Ln Sensor
Chamber 1 or Chamber 2. Do not run the 6875D Freezer/Mill without a loaded sample vial in the grinding Chamber(s) that is active (programmed to grind). -
Page 11: Grinding Vial Sizes, Adapters, And Vial Openers
The liquid nitrogen (LN) sensor is located behind a shield on the back wall of the LN tub. While in Auto Fill mode, with the 6875D Freezer/Mill connected to the LN Tank, the unit will add liquid nitrogen automatically to maintain the proper LN level. In the optional Manual Fill mode, to prevent overfilling the tub, the unit will sound with a series of “Beeps”... -
Page 12: Touchscreen Display
When the power is switched ON at the back of the mill, “Please Wait” appears during start-up as the software loads. Then the SPEX SamplePrep logo is displayed,with a web address and a brief animated representation of the Freezer/Mill. After the animation has finished, the screen will switch to the Home Screen, as shown in Figure 5. -
Page 13: Control Panel
6875D Freezer/Mill 5.2 Control Panel The Control Panel displays the programmed run parameters, as shown in Figure 6. Changes to the run settings, and to activate Mono or Dual grinding modes are made from this screen. In Mono mode the programmed run parameters will Only operate Chamber 1. If Dual mode is selected the run parameters (Figure 6), will operate Chamber 1 and Chamber 2 simultaneously. - Page 14 In the CONTROL PANEL, Mono or Dual sample mode can be selected as well as to Auto Fill or to Manual Fill the 6875D Freezer/Mill with liquid nitrogen. The STATUS BAR (top of screen) counts down the time remaining for a run. Before a run starts, the STATUS BAR will display STANDBY, as shown in Figure 6.
- Page 15 6875D Freezer/Mill 5.2 CONTROL PANEL (Cont’d) If the LN Tank is empty and unable to fill the tub with liquid nitrogen change the LN Tank and touch RESUME to begin operation. If the lid is not completely closed and latched, the screen will display LID OPEN, as shown in Figure 8.
- Page 16 6875D Freezer/Mill 5.2 CONTROL PANEL (Cont’d) Following the PRECOOL stage is the first GRINDING cycle stage. The RUN TIME line will display the time remaining in that cycle of the grinding stage. At the end of each grinding period a green check mark is placed next to that field.
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Page 17: Changing Settings
6875D Freezer/Mill 5.2.2 Adjusting the Grinding Rate While in the CONTROL PANEL screen, the only parameter that can be modified during grinding is the RATE. It is adjusted by touching the right and left arrow buttons next to the Rate field. -
Page 18: Saved Protocols
6875D Freezer/Mill 5.4 Saved Protocols The SAVED PROTOCOLS screen is shown in Figure 12. In this example, three saved protocols have been stored and named (e.g. Hair, Bone, Soft Tissue). Up to 20 Protocols can be stored. Only 10 protocols at one-time are visible on the Saved Protocols screen. To access or make visible additional protocols, or vacant protocol spaces (e.g. - Page 19 6875D Freezer/Mill 5.4 Saved Protocols (Cont’d) The Up/Down yellow arrow key on KEYBOARD allows the user to shift back and forth from upper case to lower case (the default is upper case). Touch Down arrow to switch to lower case. Or touch Up arrow to revert to upper case.
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Page 20: Operation
6.1 Standard Auto Fill When the 6875D Freezer/Mill is set up for the first run of the day, with a loaded vial(s) in the grinding Chamber(s), make sure the unit is connected to a LN Tank or LN line and the LN valve is open. -
Page 21: Manually Adding Liquid Nitrogen
Liquid nitrogen (LN) should only be added to 6875D Freezer/Mill with the lid open. To open the lid, hold the lid down with one hand, and with the other hand pull the upper end of the latch toward you. - Page 22 6875D Freezer/Mill 6.2 Manually Adding Liquid Nitrogen (Cont’d) Once more, slowly lower the lid and secure the latch. Caution: When the lid is closed and latched, nitrogen vapor which vents from the Freezer/Mill can displace the oxygen in a closed room and cause asphyxiation.
- Page 23 6875D Freezer/Mill 6.2 Manually Adding Liquid Nitrogen (Cont’d) • Grind longer. (In Manual Fill mode, 30 minutes of actual grinding time is the maximum recommended for one run, due to the liquid nitrogen capacity of the tub, but you can top off the LN and repeat the run if needed.)
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Page 24: Loading Vials Into The Freezer/Mill
6875D Freezer/Mill 6.2.5 Loading a Very Small Sample into a Vial and Keeping it Cold A similar loading technique to Section 6.2.4 can be used to grind very small samples with an upright, open pre-chilled vial. Place the sample on the chilled end plug and add a small amount of fluid such as an extraction medium or water. - Page 25 6875D Freezer/Mill 6.5 Opening, Emptying, and Cleaning Freezer/Mill Vials (Cont’d) polycarbonate center section. The best way to prevent the Extractor from jamming is to make sure both the Extractor and the threaded end plugs are dry and clean before you use them.
- Page 26 6875D Freezer/Mill 6.5 Opening, Emptying, and Cleaning Freezer/Mill Vials (Cont’d) If it is necessary to recover 100% of the sample, let the vial warm up and add some water or other liquid that will not affect the polycarbonate (Section 6.5.3). Shake the vial, empty it onto filter paper, and rinse again if necessary to recover the entire sample.
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Page 27: Checking The Liquid Nitrogen Level
6875D Freezer/Mill 6.6 Checking the Liquid Nitrogen Level In Manual Fill mode only, every time a vial is inserted into or removed from the Freezer/Mill, note the level of liquid nitrogen in the tub. If the LN level is Low the mill will not run. Add LN as needed until the unit sounds a series of “Beeps”... -
Page 28: Accessories (Electronic Version)
6875D Freezer/Mill 7.1 Run History (Cont’d) Figure 16 – Run History screen 7.2 Accessories [Electronic Version] – (Available for purchase at additional cost) To enter the Accessories Screen touch the Accessories button. In the Accessories Screen items are grouped into separate categories (e.g. Grinding Vials, Vial Racks, Extractors), as shown in Figure 17. -
Page 29: Training
6875D Freezer/Mill 7.2 Accessories [Electronic Version] (Cont’d) Figure 18 – Extractor Accessories 7.3 Training To access the Training screen touch the Training button on the Resources screen. In the Training screen the operating manual, instructions and how-to-use videos are listed. To open any of the categories listed on the screen touch the button name, as displayed in Figure 19. -
Page 30: System Settings
6875D Freezer/Mill 7.4 System Settings To access the Settings screen touch the Gear Icon. The System can be upgraded, the time display changed from 12-hour to 24-hour, and Time and Date can be entered or changed, as shown in Figure 20. Touch window to change settings then touch the SET button to lock in the figure. -
Page 31: Maintenance
6875D Freezer/Mill 8.0 MAINTENANCE The 6875D Freezer/Mill has been designed to provide trouble-free operation over a long period of time. To assure proper performance it is very important to keep the unit clean. When the Freezer/Mill is at room temperature, any spilled powders or liquids should be wiped up immediately. -
Page 32: Trouble-Shooting Guide
6875D Freezer/Mill 9.0 TROUBLE-SHOOTING GUIDE PROBLEM POSSIBLE CAUSE POSSIBLE SOLUTION No Power Make sure power cord is plugged into outlet and inlet. No Power Check outlet for power and The unit does not turn on. correct as required. Circuit Breaker tripped Turn unit OFF then ON. - Page 33 6875D Freezer/Mill 9.0 TROUBLE-SHOOTING GUIDE (Cont’d) PROBLEM POSSIBLE CAUSE POSSIBLE SOLUTION Water in vial has frozen the Remove vial and replace vial impactor. and contents with a dry unit or tap ends of the vial on counter to free impactor.
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Page 34: Warranty
30 days or for the remaining original warranty period (whichever is greater) for the item that was repaired or replaced. Items not produced by SPEX SamplePrep LLC carry the manufacturer’s warranty only. The Freezer/Mill wear parts include the dual magnetic coils. -
Page 35: Instrument Disposal
6875D Freezer/Mill 11.0 INSTRUMENT DISPOSAL In accordance to the EU Directive 2012/19/EU covering Waste Electrical and Electronic Equipment, all equipment with the disposal symbol must not be disposed of with general waste. (See Figure 22) Disposal Label is located on the back of unit. -
Page 36: Contact Us
Fax: +44 (0) 208 204 6654 Website: www.spexeurope.com E-mail: spexeurope@spex.com Outside the United States and Europe, contact the SPEX SamplePrep representative from whom you purchased your equipment. A list of our current representatives is on our website, www.spexsampleprep.com. SPEX SamplePrep LLC Page 36 of 36...
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