palintest Wagtech Potatest + Manual
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palintest Wagtech Potatest + Manual

Rapid response portable water quality laboratory
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Wagtech
®
+
Potatest
®
Rapid Response Portable
Water Quality Laboratory
Palintest
®
ZI PTW 10005

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  • Page 1 Wagtech ® Potatest ® Rapid Response Portable Water Quality Laboratory Palintest ® ZI PTW 10005...
  • Page 2 ® ® Acquired by Palintest in 2011, the manufacture and support of the Wagtech portable water quality laboratory range has now been integrated into the Palintest ® product family. Further information regarding the Wagtech ® product range can be found at: www.palintest.com...

  • Page 3: Table Of Contents

    Contents Chapter Page Kit Layout Introduction Preparation Taking a Sample Sample Processing - Membrane Filtration Incubation and Incubator Operation Microbiological Results Water Safety Kit (WSK) Appendix 1 - Field Testing Checklist - Hints Appendix 2 - Troubleshooting Appendix 3 - Technical Specifications Appendix 4 - Reagents and Consumables...

  • Page 4: Kit Layout

    Kit Layout Potatest + Water Test Kit, Including Water Safety Kit ® ® Fig 1. Potatest + Water test kit with water safety kit removed from lid and opened to show contents. Coloured circles indicate the chapter colour in which their use is explained.
  • Page 5 Kit Layout Function Equipment Preparing the Membrane Lauryl Sulphate Broth (MLSB) Media/Petri Dishes Media Measuring Device (MMD) Absorbent Pads Pad Dispenser Petri Dishes (in incubator) Membrane Filtration Membrane Filtration Unit Hand Vacuum Pump Forceps 47mm Membrane Filters Incubation and Incubator Incubator Operation Mains Power Supply Battery...

  • Page 6: Introduction

    This instruction manual is also available in French, Spanish and Mandarin. Additional advice and training is available upon request. Contact us directly at support@palintest.com or via your local representative. 2.1 Before You Use Your Kit Thermotolerant Coliforms (sometimes called Faecal Coliforms).
  • Page 7 Introduction Total Coliforms refers to a large group of In simple terms the process is as follows: Gram-negative, rod-shaped bacteria that share A known volume of sample water (100ml several characteristics. The group includes or less for highly contaminated samples) is Thermotolerant Coliforms and bacteria of filtered using a specific piece of apparatus faecal origin, as well as some bacteria that...
  • Page 8 Introduction A vacuum hand pump attached to the MFU Useful to Know creates suction that pulls the sample water through a sterile membrane filter that sits • Always wash your hands before in place in the MFU. carrying out your microbiological analysis, and again after handling This membrane filter has small pores in it potentially contaminated materials...

  • Page 9: Preparation

    Preparation 3.0 Preparation Every effort should be made to keep the kit and all its components clean and free from contamination. At all times you should work in a manner that limits the chance of cross contamination of your samples. 3.1 Aseptic Procedure Useful to Know There are specific techniques and methods...
  • Page 10 Preparation 3.2 Sterilisation of the for sterilisation (see below), which will Membrane Filtration Unit leave a small gap between the bottom of the filter funnel and the silicone base. Upon receipt of your water test kit the Membrane Filtration Unit (MFU) must be washed thoroughly and then dried with a clean cloth or paper towel.
  • Page 11 Preparation 3.3 Sterilisation of the 3 Remove the lid Aluminium Petri Dishes of the petri dish and dispense a Your Wagtech test kit is supplied as standard pad into the dish with a set of aluminium petri dishes. These by sliding back are used during the microbiological analysis.
  • Page 12 Preparation 3.6 Preparing Culture Media 6 Remove the MMD, allow to cool. in the Field using the Media 7 Once cool, unscrew the blue lid/spoon of Measuring Device (MMD) the MMD. Take ten level spoonfuls of media Items Required: from the 38.1g container of the MLSB and add to the MMD.
  • Page 13 Preparation 3.7 Tyndallisation of MMDs 3.8 Storage of Liquid Media In the event an autoclave or pressure cooker The sterile MLSB should be stable for up is not available then the sterilisation of the to 6 months if stored in a refrigerator. If no MMD containing the liquid MLSB media can refrigerator is available the media can be be carried out using a pan of boiling water.
  • Page 14 Preparation Useful to Know • Growth or culture media is a substance designed to support the growth of microorganisms (bacteria). The media is a vital part of the microbiological water quality test • There are different types of media for growing different types of bacteria.

  • Page 15: Taking A Sample

    Taking a Sample 4.0 Taking A Sample The optimum volume of sample is that which will allow the most accurate enumeration of bacteria. The technique of membrane filtration is unsuitable for natural waters containing very high levels of suspended material, sludges and sediments, all of which could block the filter before an adequate volume of water has been filtered.

  • Page 16: Sample Processing - Membrane Filtration

    Sample Processing - Membrane Filtration 5.0 Membrane Filtration of the Sample Items Required: • Membrane Filtration Unit • MMD containing Liquid MLSB Media • Pistol Grip Hand Vacuum Pump • Petri Dishes containing Absorbent Pad • Forceps/Tweezers • Methanol (not supplied) •...
  • Page 17 Sample Processing - Membrane Filtration 6 Lock the membrane filter in place by 9 Discard the excess water from the sampling pushing the filter funnel firmly into cup then insert the filter funnel/base position in the blue rubber base. Take assembly into position in the sampling cup.
  • Page 18 Sample Processing - Membrane Filtration 12 When all of the sample has been filtered, 16 Lift the petri dish containing the detach the vacuum pump and remove absorbent pad and hold between the filter funnel from the rubber base. thumb and forefinger. The membrane filter is now ready to be removed and placed in the petri dish containing the absorbent pad and...
  • Page 19 Sample Processing - Membrane Filtration 19 Use the sterile forceps to remove the Environmental exposure can cause coliforms membrane filter from the filtration unit. to become physiologically stressed. The resuscitation period allows coliforms to recover before culturing. With this in mind it is essential to plan testing throughout the day, particularly if visiting multiple sample sites.

  • Page 20: Incubation And Incubator Operation

    Incubation and Incubator Operation 6.0 Operation of Incubator The Potatest ® + incubator is a high performance field incubator designed to deliver reliable Total and Thermotolerant Coliform results in even the most extreme circumstances. Providing at least 5 cycles of incubation under standard conditions when battery powered, the incubator is simple to operate and provides performance data throughout the incubation cycle.
  • Page 21 Incubation and Incubator Operation 6.2 Recharging the 12V DC Battery • A red LED in the right hand corner of the incubator lid indicates that power is supplied Note: For optimal recharging, and the incubator is on. The backlight will switch off the incubator.
  • Page 22 Incubation and Incubator Operation 6.3.2 Setup Menu • When the correct character is shown, release the key. The cursor moves to the • The Setup menu allows the user to set next character automatically, up to a the incubator preferences and validate maximum of 12 characters performance •...
  • Page 23 Incubation and Incubator Operation Set Date To set the Date: • Highlight ‘Set Date’ and press ‘OK’ • Use the UP/DOWN keys to set the day. When correct press ‘OK’ • Use the UP/DOWN keys to set the month. When correct press ‘OK’ The Check Calibration function provides a •...
  • Page 24 Incubation and Incubator Operation 6.3.3 Incubation Menu There are five standard prompt cards with corresponding audio, both indexed by letter The ‘Incubation’ menu is accessed from and number. the ‘MODE’ screen, confirms the incubator settings and starts the incubation cycle. The Prompt Cards are grouped as follows: •...
  • Page 25 Incubation and Incubator Operation 6.4 Incubating Samples • The incubator has a default warm-up period of 30 minutes at the start of the The incubation of samples can begin once incubation process. During this phase the the correct resuscitation period has been incubator will display ‘warming up’...
  • Page 26 • Before selecting function, click • For the latest information and more appropriate flag to change language, detailed guidance notes, see the if required Know section of the Palintest website...
  • Page 27 Incubation and Incubator Operation 6.6.2 Language Update New prompts in local languages and dialects can be uploaded using the Pota Pal app and To update languages: Audacity software functionality. The Android • Start the Pota Pal app app also has the ability to record prompts. •...
  • Page 28 The incubator temperature has exceeded the set-point for a significant period. Remove For service please contact your local from direct sunlight or high temperature Palintest representative or email to conditions and accept the error. support@palintest.com.

  • Page 29: Microbiological Results

    Microbiological Results 7.0 Microbiological Results Bacteria in water are generally not present individually but as clumps or in association with particulate matter. When enumerating bacteria in water it is not the number of individual bacteria present which are counted but the number of clumps of bacteria or the particles and their associated bacteria.

  • Page 30: Water Safety Kit (Wsk)

    Water Safety Plans (WSPs) and their role in assessing and managing risk throughout each step of the drinking water supply process. More information on Water Safety Plans and their part in the Water Safety Framework can be found at www.palintest.com...
  • Page 31 Water Safety Kit (WSK) 8.1 Jackson Turbidity Tubes 8.2.1 Taking pH Measurements The two-part Turbidity Tube provides a Remove the protective cap and press simple and intuitive method of determining ON/OFF to switch the meter on. turbidity using a visual approach. Higher Dip the electrode into 2-3cm of sample, rotate values of turbidity can be determined using gently and wait for the reading to stabilise.
  • Page 32 Water Safety Kit (WSK) 8.2.3 Calibrating Temperature Chlorine Testing Procedures ON/OFF to switch the meter on. 8.3.1 Free Chlorine Press HOLD/ENT Select the Chlorine disk with the desired Press and hold CAL for 3 seconds to range and place in the Contour body. enter temperature calibration mode.

  • Page 33: Appendix 1 - Field Testing Checklist - Hints

    Appendix 1 - Field Testing Check List - Hints Note: Field testing requires careful planning Ensure you have considered the following When you are in the field: additional items that may be required in • Find a flat area! Always place kit on a ®...

  • Page 34: Appendix 2 - Troubleshooting

    Should any condition arise requiring be displayed. Select ‘Accept’ to continue with maintenance or service, please contact the incubation cycle but find an additional your local Palintest representative or via power source to avoid losing power during support@palintest.com microbiological analysis. Select ‘Back’ to change The Wagtech incubator is designed to power source prior to starting incubation.

  • Page 35: Appendix 3 - Technical Specifications

    + incubator is designed for incubation of microbiological samples in petri dishes. Follow the instructions regarding connection of power cables carefully and only use genuine Palintest cables/chargers. Using the Potatest ® + incubator in a manner not specified by Palintest may affect safe performance and invalidate warranty.

  • Page 36: Appendix 4 - Reagents And Consumables

    Appendix 4 - Reagents and Consumables Membrane Filtration Consumables Part Number Description PTW 10452 Membrane Lauryl Sulphate Broth, 500g PTW 10454 Membrane Lauryl Sulphate Broth, 38.1g PTW 10456 1.92g Membrane Lauryl Sulphate Broth, pack of 25 PTW 10459 Membrane Filters, 47mm diameter, 200 pack PTW 10460 Absorbent Pads and Membranes, 200 pack PTW 10461...
  • Page 37 Appendix 4 - Reagents and Consumables Reagents Part Number Description AKW 011 Free Chlorine (DPD 1), 200 tests, Polypropylene Carton AKW 031/1 Total Chlorine (DPD 3), 200 tests, Polypropylene Carton AKW 031 Free and Total Chlorine (DPD 1 & 3), 200 tests, Polypropylene Carton AK 011 Free Chlorine (DPD 1), 250 tests, Refill Pack AK 031/1...
  • Page 38 Palintest Ltd Palintest House, Kingsway, Team Valley, Gateshead, NE11 0NS Tel: +44 (0)191 491 0808 Fax: +44 (0)191 482 5372 sales@palintest.com (UK) export@palintest.com (International) Palintest Australia and Asia Pacific 1/53 Lorraine Street, Peakhurst Business Centre, Peakhurst, NSW 2210, Australia Tel: +61 1300 131516 Fax: +61 1300 131986 palintest@palintest.com.au...

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