Olympus BX-RLAA Instructions Manual
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Olympus BX-RLAA Instructions Manual

Olympus BX-RLAA Instructions Manual

Motorized reflected light brightfield/darkfield illuminator
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INSTRUCTIONS
BX-RLAA
MOTORIZED REFLECTED LIGHT
BRIGHTFIELD/DARKFIELD ILLUMINATOR
This instruction manual is for the Olympus Motorized Reflected Light Brightfield/Darkfield Illuminator
Model BX-RLAA. The BX-RLAA is capable of brightfield and darkfield observations under the reflected
light when it is installed on the BX61TRF system microscope frame. To ensure the safety, obtain opti-
mum performance and to familiarize yourself fully with the use of this unit, we recommend that you
study this manual thoroughly before operating the microscope. Retain this instruction manual in an
easily accessible place near the work desk for future reference.
A X 7 6 2 3

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  • Page 1 This instruction manual is for the Olympus Motorized Reflected Light Brightfield/Darkfield Illuminator Model BX-RLAA. The BX-RLAA is capable of brightfield and darkfield observations under the reflected light when it is installed on the BX61TRF system microscope frame. To ensure the safety, obtain opti- mum performance and to familiarize yourself fully with the use of this unit, we recommend that you study this manual thoroughly before operating the microscope.

  • Page 3: Table Of Contents

    — Be sure to read this chapter for safe use of the equipment. — 1 SYSTEM DIAGRAM 2 NOMENCLATURE OF BX-RLAA 3 MOTORIZED REFLECTED LIGHT BRIGHTFIELD/DARKFIELD OBSERVATION PROCEDURE 4 USING THE CONTROLS OF BX-RLAA 7-11 Selecting the Observation Path Centering the Field Iris Diaphragm (FS)

  • Page 4: Important - Be Sure To Read This Chapter For Safe Use Of The Equipment

    IMPORTANT The BX-RLAA motorized reflected light brightfield/darkfield illuminator can be controlled electrically by the BX-UCB control box and U-HSTR2 hand switch (the BX-RLAA can also be interlocked with other motorized modules by running the BX2 software for PC). Motorized features: ·...
  • Page 5 BX-RLAA Maintenance and Storage 1. To clean the lenses and other glass components, simply blow dirty away using a commercially available blower and wipe gently using a piece of cleaning paper (or clean gauze). If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available absolute alcohol.

  • Page 6: System Diagram

    SYSTEM DIAGRAM Motorized Reflected Light Reflected Brightfield/Darkfield Illuminator Light Lamp BX-RLAA Housing Module Motorized Revolving Nosepiece Connection cable Stage for Metallurgic Specimens or Stage Plate* Connection cable Microscope frame BX61TRF Control Box BX-UCB Hand Switch U-HSTR2 * To improve the ease of use of the stage, replace it with a stage for metallurgic specimens...

  • Page 7: Nomenclature Of Bx-Rlaa

    BX-RLAA NOMENCLATURE OF BX-RLAA Polarizer insertion slot (Page 15) Field iris diaphragm (FS) knob (Pages 7-8) Analyzer insertion slot (Page 15) Filter insertion slots (Page 11) AS centering screw (Pages 8-9) FS centering screws (Page 7)

  • Page 8: Motorized Reflected Light Brightfield/Darkfield Observation Procedure

    MOTORIZED REFLECTED LIGHT BRIGHTFIELD/DARKFIELD OBSERVATION PROCEDURE }The following flow shows the basic operating procedure for reflected light brightfield or darkfield observation. The operat- ing procedures for polarized light and Nomarski DC observations will be described separately in their descriptions. (Controls Used) (Page) (Controls Used) (Page)
  • Page 9 BX-RLAA … œ ™ Œ Motorized Reflected Light Brightfield/Darkfield ƒ Illuminator › BX-RLAA Control Box ³ BX-UCB Motorized Revolving Nosepiece ‰ † ‹ I (ON) š Microscope BX61 Š ‡ Hand Switch ² U-HSTR2 } Make a photocopy of the observation procedure pages and post it near your microscope.

  • Page 10: Using The Controls Of Bx-Rlaa

    USING THE CONTROLS OF BX-RLAA Selecting the Observation Path (Fig. 1) ² Select the mirror for the desired observation method by pressing the BF button @ or DF button ² on the hand switch. BF: Reflected light brightfield observation DF: Reflected light darkfield observation Fig.

  • Page 11: Centering The Aperture Iris Diaphragm (As)

    BX-RLAA 6. Open the field iris so that its image is almost the same size as (i.e. sub- scribes) the field of view. Field iris diaphragm image Using the Field Iris Diaphragm {In reflected light brightfield observation The field iris diaphragm adjusts the illuminated area to obtain an image with high contrast.
  • Page 12 3. Remove the eyepiece, look into the eyepiece sleeve and press the AS – button 7 on the hand switch to set the aperture at around 70%. 4. If the center of the iris diaphragm is deviated, center it by rotating the two AS centering screws (ƒ...

  • Page 13: Using The Interlocked Nd Filter

    BX-RLAA Using the Interlocked ND Filter (Fig. 5) }The ND filter is interlocked with the brightfield (BF) light path switching and makes it possible to reduce glare when the darkfield (DF) path is switched to the brightfield (BF) path. Removing the Interlocked ND Filter }The ND filter has been interlocked at the factory.

  • Page 14: Using The Filters

    Using the Filters (Fig. 6) }Engage the optimum filter sliders for the purpose of observation in the two filter insertion slots @ so that the filter surfaces with indication comes on the front. The first click position is the idle position and the second click engages the filter in the light path.

  • Page 15: Stage For Metallurgical Specimens

    BX-RLAA STAGE FOR METALLURGICAL SPECIMENS }To improve the ease of operation, it is recommended to use a stage for exclusive use with metallurgical specimens or replace the biological specimen holder with that for metallurgical use. Placing the Specimen }The maximum load capacities are as follows.
  • Page 16 [U-SIC4R2/SIC4L2 Stage Only] ² Observation with the U-WHP2 Wafer Holder Plate (Fig. 8) 1. Place the BH2-WHR43 rotary wafer holder (for 3- or 4-inch) ² on the U- ³ WHP2 wafer holder plate @. 2. Place a 3-inch or 4-inch wafer on the BH2-WHR43 and observe the wafer. The wafer holder plate can be rotated with the knob ³.

  • Page 17: Using The Y-Axis Movement Lock Lever

    BX-RLAA Using the Y-Axis Movement Lock Lever (Fig. 10) }This mechanism is provided only with the U-SIC4R2/SIC4L2 stage. 1. When the Y-axis movement lock lever @ is locked by setting the lever in the direction of the arrow, the Y-axis movement is locked, making it pos- sible to scan only in the X-axis direction.

  • Page 18: Observation Methods (Using Bx-Rlaa)

    OBSERVATION METHODS (Using BX-RLAA) 6-1 Reflected Light Brightfield/Darkfiled Observation See “MOTORIZED REFLECTED LIGHT BRIGHTFIELD/DARKFIELD OBSERVATION PROCEDURE” on page 5. 6-2 Reflected Light Nomarski DIC (Differential Light Contrast) Observation # The performance of polarizer may deteriorate when it has been exposed to light for a long period (about continu- ous 2000 hours).
  • Page 19 BX-RLAA Using the Joint Plate (Fig. 12) … When the U-AN360-3 analyzer ƒ and U-PO3 or U-POTP3 polarizer … are coupled by using the joint plate | provided with the polarizer and tighten- ing the clamping knobs on it, the analyzer and polarizer can be engaged ƒ...
  • Page 20 3. With the U-DICRH or U-DICRHC slider that does not have the slide lever, the applicable objectives are as follows. DIC Slider Applicable Objectives U-DICRH MPLFLN/MPLFLN-BD series UIS2 MPLAPON series UMPlanFl/UMPlanFl-BD series MPlanFl-BD series MPlanApo20X, 100X U-DICRHC LMPLFLN/LMPLFLN-BD series UIS2 LMPlanFl/LMPlanFl-BD series LMPlanApo/LMPlanApo-BD series...
  • Page 21 BX-RLAA Observation Method (Fig. 13) 1. Place the specimen on the stage and adjust the focus by moving the stage up or down. 2. Adjust the field iris diaphragm so that its image circumscribes the field of view. 3. The contrast may sometimes be enhanced by closing the aperture iris diaphragm slightly.
  • Page 22 U-DICRH 1. Adjust the background contrast by turning the prism movement knob | on the DIC slider as described below. (Fig. 13) 2. When the prism movement knob on the U-DICRH DIC slider is turned, the interference color in the background varies from -100 to 100 nm. Set the retardation which can provide best contrast.

  • Page 23: 6-3 Reflected Light Simplified Polarized Light Observation

    BX-RLAA Switching to Brightfield/Darkfield Observation (Fig. 13) 1. Loosen the mounting knob @ on the front of the DIC revolving nosepiece, gently pull out the DIC slider ² until it clicks, then clamp by tightening the mounting knob. 2. Slide the analyzer (U-AN360-3) and polarizer to disengage them from the light path.

  • Page 24: Specifications

    · Observation method switching: Motorized BF/DF switching Illuminator · Aperture iris diaphragm: Motorized opening/closing ( 0.4 to 11.1 mm), centering possible BX-RLAA · Field iris diaphragm: Manual knob opening/closing, centering possible Power Source: · Filter slider insertion slots: x 2 ·...

  • Page 25: Optical Characteristics "Uis2 Series

    (as shown in the diagram on the right). UIS marking NA (Number of Aperture) NOTE Brightfield/darkfield Refer to the latest catalogue or consult Olympus for the updated infor- application mation on the eyepieces and objectives that can be combined with Cover glass thickness this unit.
  • Page 26 Eyepieces Optical Cover characteristics Resolu- WHN10X (FN22) SWH10X (FN26.5) glass Magnifi- W.D. tion N.A. thick cation (mm) Depth Field Depth Field (μm) Total Total ness of focus of view of focus of view mag. mag. (mm) (μm) (mm) (μm) (mm) Series Marking 0.15...
  • Page 27 BX-RLAA Eyepieces Optical Cover characteristics Resolu- WHN10X (FN22) SWH10X (FN26.5) glass Magnifi- W.D. tion N.A. thick cation (mm) Depth Field Depth Field (μm) Total Total ness of focus of view of focus of view (mm) mag. mag. Series Marking (μm) (mm) (μm)
  • Page 28 Significance of Objective Name (Examples) (Plan) None : Brightfield : Brightfield/darkfield BDP : Brightfield/darkfield or polarized : IR light Figure : Magnification None : UIS : UIS 2 None : Achromat, or aberration correction with 2 wavelengths (red and bleu). : Semi-Apochromat, or color aberration correction with visual wave- lengths (bluish purple to red).
  • Page 29 BX-RLAA Glossary of Terms Used in the Optical Characteristics Table Working distance (WD) : The distance from the top of specimen and the front lens of objective. Number of aperture (NA) : Important figure determining the objective characteristics (resolution, focal depth and bright- ness).

  • Page 30: Assembly

    }For assembly of the BX-UCB control box and U-HSTR2 hand switch, refer to their instruction manual. # If the connector of the U-UCD8A motorized universal condenser is attached to the BX-UCB, the BX-RLAA motor- ized reflected light illuminator cannot work normally. Be sure to unplug the connector if it is connected.
  • Page 31 Preparation for Mounting the Motorized Revolving Nosepiece (Fig. 14) }With certain motorized revolving nosepiece series, the connector of the BX-RLAA reflected light illuminator should be unlocked before operation. U-D6REM/5BDREM The connector can be connected by mounting the revolving nosepiece in the same way as manual revolving nosepiece.
  • Page 32 Attaching the Illuminator (Fig. 15) ² }Attach the illuminator using the Allen wrench ( ) provided with it. }Remove the caps of the mounting screws ² using a pair of tweezers, etc. 1. Place the illuminator @ on the mounting surface by slightly deviating the 4 ³...
  • Page 33 MEMO...
  • Page 34 MEMO...
  • Page 36 Shinjuku Monolith, 3-1, Nishi Shinjuku 2-chome, Shinjuku-ku, Tokyo, Japan Wendenstraße 14-18, 20097 Hamburg, Germany 3500 Corporate Parkway, P.O. Box 610, Center Valley, PA 18034-0610, U.S.A. One Corporate Drive, Orangeburg, NY 10962, U.S.A. 491B River Valley Road, #12-01/04 Valley Point Office Tower, Singapore 248373 31 Gilby Road, Mount Waverley, VIC., 3149, Australia 5301 Blue Lagoon Drive, Suite 290 Miami, FL 33126, U.S.A.

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