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4. Troubleshooting and FAQs
Error messages
Error Message
Buffer Error
No Tank
Pump Error
System Error
FAQ
Description
Strong background
Weak signal
Fluorescent detects
only strong signal
around spacer
Possible causes
Can't read cassette chip/no
cassette
Cassette not secured in
position
Pump malfunction
System malfunction
Possible causes
Insufficient wash
1. Antibody is too
diluted
2. Instrument needs
cleaning
3. Insufficient
antibody
incubation time
Weak target signal
contrasted out by
interference
Solutions
Replace cassette/ insert
cassette
Check Pins and Reinsert
cassette securely
Contact GenScript
Contact GenScript
Solutions
1. Increase number
2. Increase pump
1. Reduce antibody
2. Clean eZwest Lite
3. Increase antibody
1. Adjust detection
2. Cover spacer
20
of wash cycles (set
to 5 cycles for 3
minutes each)
flow per wash
cycles (set to
normal)
dilution factor
instrument
incubation time or
reduce incubation
pump flow
area to omit
spacer position
signal region with
paper or tape
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