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Pictured on Cover: BACTRON900 (BACTRONIV-900) BACTRON600 (BACTRONIV) BACTRON300 (BACTRONII) These units are TÜV CUE listed as anaerobic chambers for professional, industrial, or educational use where the preparation or testing of materials is done at an ambient air pressure range of 22.14 –...
TABLE OF CONTENTS INTRODUCTION ............................6 General Safety Considerations ......................... 6 Engineering Improvements ........................7 Contacting Assistance ..........................7 RECEIVING YOUR BACTRON ........................8 Inspecting the Shipment ..........................8 Orientation Photos............................. 9 Record Data Plate Information ........................ 12 Temperature Reference Sensor Device ....................12 Catalyst Oven ............................
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Volatile Compound Scrubber and Rejuvenation Cycle ................55 Attaching Equipment to the Chamber Accessory Outlets ............... 55 Deionized and Distilled Water ......................... 56 Humidifying Incubators ..........................56 Pressure Unit Conversion ........................56 USER MAINTENANCE ..........................58 Daily Maintenance ........................... 59 Normal Gas Consumption ........................
INTRODUCTION Thank you for purchasing a Shel Lab BACTRON® Anaerobic Chamber Workstation. We know that in today’s competitive marketplace, customers have many choices when it comes to constant temperature equipment. We appreciate you choosing ours. Our continued reputation as a leading laboratory product manufacturer rests with your satisfaction.
Therefore, some changes, modifications, and improvements may not be covered in this manual. If your unit’s operating characteristics or appearance differs from those described in this manual, please contact your Shel Lab dealer or distributor for assistance.
The unit should come with an Installation and Operation Manual and a Certificate of Compliance. Verify that the correct number of items are included with the unit: Anaerobic Monitoring Arm Port Door Arm Port Door BACTRON300 / 900 BACTRON600 Shelf Spacers (3) Arm Port Door Strips (5 packets) Left...
RECEIVING YOUR BACTRON (CONTINUED) RIENTATION HOTOS Figure 1: BACTRON900 Catalyst Cartridge LED Lamp Workspace Chamber Side Storage Incubator Workspace Incubator Control Panel Incubator Gas and Vacuum Ports Main Control Panel (Side Storage Incubator) Pass Box Manometer Left Arm Port Right Arm Port Figure 2: BACTRON600 Catalyst Cartridge Manometer...
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Incubator Chamber Gas and Vacuum Ports Control Panel Pass Box Manometer Left Arm Port Right Arm Port Figure 4: BACTRON300 Power Outlet Power Cord Inlet Vacuum Pump with Fuse Figure 3: Power Supply Panel, All Units (located on back) Fuse Holder Vacuum...
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RECEIVING YOUR BACTRON (CONTINUED) Workspace Chamber Orientation (All Units) Catalyst Cartridge Data Plate Figure 5: Workspace Chamber Layout Pass Box Orientation Pass Box Inner Door Gasket Figure 6: Pass Box Inner Door and Sliding Shelf The pass box-style airlock is provided with an integral sliding shelf to facilitate material transfers. Small items may be introduced into the chamber via the Arm Port Doors and Sleeve Assemblies using proper sleeve technique.
RECEIVING YOUR BACTRON (CONTINUED) ECORD LATE NFORMATION Locate the data plate in the workspace chamber above the inner pass box door. The data plate contains the BACTRON model number and serial number. Enter this information below for future reference. Date Plate Information Model Number Serial Number EMPERATURE...
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Place 5 unopened anaerobic monitoring packets in the workspace chamber, page 23 Install shelf spacers in the workspace incubator (BACTRON300 and BACTRON900), page 23 Prepare the incubators by leaving the incubator doors slightly ajar (0.5 inch / 1cm), page 23 ...
Operating the BACTRON outside of these conditions may adversely affect its incubator temperature range and stability. For conditions outside of those listed above, please contact your distributor or Shel Lab Sales to explore other options suited to your laboratory or production environment.
The wall power source for the BACTRON must match the voltage and ampere requirements listed on the unit data plate. These units are intended for 110 - 120V 50/60 Hz applications at the following amperages: Model Amperage 9 Amps BACTRON300 11 Amps BACTRON600 14 Amps BACTRON900 ...
INSTALLATION (CONTINUED) IFTING AND ANDLING The BACTRON is heavy. Use appropriate powered lifting devices. Follow these guidelines when lifting and handling the BACTRON workstation: Lift the BACTRON only from its bottom surface. Doors, handles, and knobs are not adequate for lifting or stabilization. ...
INSTALLATION (CONTINUED) UPPLY EQUIREMENTS Warning : Never exceed a 5% hydrogen concentration inside the anaerobic workspace chamber. La concentration d’hydrogène ne doit pas dépasser 5% dans la chambre anaérobie. Avertissement: AMG (Anaerobic Mixed Gas) - Required The BACTRON maintains an anaerobic environment in the workspace chamber in part through a catalytic oxygen-capturing scrubbing process.
INSTALLATION (CONTINUED) ONNECT THE UPPLY Warning : Never exceed a 5% hydrogen concentration in the unit chamber. La concentration d’hydrogène ne doit pas dépasser 5% dans Avertissement: la chambre anaérobie. Single Gas Configuration T-Fitting Figure 8: T-Fitting Filtered Gas Tubing Kit Carry out the following steps to connect a single cylinder Figure 9: Single Gas Configuration of anaerobic mixed gas.
INSTALLATION (CONTINUED) ONNECT TO A ACUUM UPPLY OURCE The BACTRON requires a supply of vacuum to perform pass box and arm port cycles. The BACTRON may be connected to either a building vacuum supply system, or the vacuum pump provided with the unit. Connect to a Building Vacuum Supply System An in-house system must be capable of evacuating a minimum of 25 inches (63.5 cm) of mercury.
INSTALLATION (CONTINUED) ONNECT THE EDAL ONTROLS The foot pedal controls allow the user to apply vacuum and AMG to the arm ports in order to cycle the ports and attached sleeves. Cycling is done prior to entering the chamber workspace. Connect the foot pedal supply line to the arm port door assemblies 1.
Clean, disinfect, and place the following items in the workspace chamber: The arm port doors The incubator bottom shelf spacers (BACTRON300 and BACTRON900) Arm port door stands (BACTRON600 only) The petri dish racks. These can be placed on the top shelf of the workspace chamber incubator or the BACTRON600 workspace shelves during the setup.
HELF PACERS BACTRON300 and BACTRON900 Install the three (3) metal spacers included with the BACTRON300 and 900 in the workspace chamber incubator. These spacers ensure even heat distribution and uniformity. Set the spacers on the bottom shelf of the workspace chamber incubator, side by side, with the “SPACER”...
INSTALLATION (CONTINUED) NSTALL THE OORS Install the arm port doors in the arm ports to seal the chamber. The arm port doors should already be inside the workspace chamber. 1. Turn each silver locking bar to a roughly 45° position. 2.
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GRAPHIC SYMBOLS The BACTRON is provided with multiple graphic symbols located on its exterior and interior surfaces. The symbols identify hazards and the functions of the adjustable components, as well as important notes found in the user manual. Symbol Definition Indicates that you should consult your user manual for further instructions.
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GRAPHIC SYMBOLS (CONTINUED) Symbol Definition Indicates Potential Shock Hazard Signale danger électrique Indicates the unit should be recycled (Not disposed of in land-fill) Indique l’appareil doit être recyclé (Ne pas jeter dans une décharge) Indicates an anaerobic environment in the pass box Indique un environnement anaérobie dans le sas Indicates an aerobic environment in the pass box Indique un environnement aérobie dans le sas...
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The main panel on all BACTRON models controls power to the workstation and the operation of pass box cycles. It also indicates injections of AMG into the workspace chamber. Main Panel Incubator Controls BACTRON300 The Incubator controls on the main panel control the operations of the workspace incubator. BACTRON600 The Incubator controls on the main panel control the operations of the side storage incubator.
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CONTROL PANEL OVERVIEW (CONTINUED) Main Control Panel Continued Power Switch The power switch controls all power to each workstation and its systems. Power is supplied when the switch is in the ( I ) on position and illuminated. Chamber Gas Light This light illuminates when anaerobic mixed gas is being injected into the workspace chamber.
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CONTROL PANEL OVERVIEW (CONTINUED) Main Control Panel Continued Automatic Start / Reset Switch The Automatic Start / Reset switch initiates or aborts the pass box auto cycle used to purge aerobic atmosphere from the pass box. Pressing then releasing on the start side (left) initiates the auto cycle.
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CONTROL PANEL OVERVIEW (CONTINUED) Figure 24: BACTORN900 Workspace Incubator Control Panel The workspace incubator control panel on the BACTRON900 controls the operations of the workspace chamber incubator and Over Temperature Limit system. Workspace Incubator Display and Control Labeled Set Temperature, the display shows the current air temperature in the workspace incubator accurate to within 0.1C.
OPERATION HEORY OF PERATION AND AJOR OMPONENT UNCTIONS Achieving Anaerobic Conditions Hydrogen Oxygen Positive Active Pressure Catalyst Figure 6: Atmosphere Control Measures The BACTRON workstation is designed to establish and maintain an anaerobic atmosphere suitable for clinical cultivation of anaerobic bacteria. This atmosphere is initially achieved through a purge of the workspace chamber using a flow of anaerobic mixed gas (AMG).
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Incubators BACTRON300 and BACTRON900 are each provided with a cabinet style incubator in the workspace chamber. The BACTRON600 and BACTRON900 come with two rotating shelves (Lazy Susans) in a side storage incubator.
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OPERATION (CONTINUED) The Over Temperature Limit System The OTL is a mechanical heating cutoff included with each incubator, and which operates independently of the incubator microprocessor. It is intended to be set by the user to approximately 1°C above the current operating temperature set point. The OTL prevents runaway heating in the event that the main controller fails while in its heating mode.
8 hours at 200C will be needed to reactivate the catalyst. The BACTRON requires a continual supply of AMG to set up and operate. The BACTRON300 needs approximately 200 psi of AMG for its purge of the workspace chamber atmosphere. The BACTRON600 and BACTRON900 require around 400 psi of AMG for the setup purge of the chamber and side storage incubator.
GAS 1 IN port 2. Note the reading on the AMG gas regulator. a. The BACTRON300 requires approximately 200 psi of AMG for the initial setup purge. b. The BACTRON600 and BACTRON900 need around 400 psi of AMG for the initial setup purge.
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OPERATION (CONTINUED) Establish an Anaerobic Atmosphere (Continued) 6. Verify the interior and exterior pass box doors are closed and securely latched. 7. Check that the arm port foot pedal control assembly is connected to both arm ports and the VAC and GAS out ports on the BACTRON. ...
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OPERATION (CONTINUED) Establish an Anaerobic Atmosphere (Continued) 12. Reinstall the right arm port door and secure it, as per the Installing the Arm Port Doors procedure on page 24. a. Once secured, loosen the knob on the right arm port door by about half a turn.
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Setting the Incubator Temperature procedure on page 47. 17. End state BACTRON300. When the purge has gone through 200 psi of AMG or BACTRON300 gone on for 2 hours (whichever happens first), tighten and secure the 200 psi AMG right arm port door. Then carry out the...
OPERATION (CONTINUED) TTACH THE LEEVE SSEMBLIES Prior to verifying the achievement of an anaerobic atmosphere, attach and secure both sleeve assemblies to the arm ports. This allows access to the chamber through the ports without introducing oxygen from the outside atmosphere. Figure 26: Sleeve Assembly Installation Start with either arm port.
4. Stow the arm ports doors, one at a time. a. BACTRON300 and BACTRON900 – place the arm port doors one at a time in the hanging door shelves on the bottom of the workspace chamber incubator.
OPERATION (CONTINUED) OVING IN THE RESSURIZED HAMBER There is a minor, but noticeable feeling of resistance while working in the overpressure environment of the workspace chamber. Moving in the chamber will temporarily increase the chamber pressure. Large and fast movements can cause the manometer to bubble as anaerobic atmosphere is forced out of the BACTRON.
OPERATION (CONTINUED) ERIFYING AN NAEROBIC TMOSPHERE This procedure verifies that an anaerobic atmosphere has been established prior to placing anaerobe samples in the workstation. 1. After entering the workspace chamber, open one of the Oxoid brand anaerobic monitoring strip packages. 2.
OPERATION (CONTINUED) ROUBLESHOOTING XYGEN IN THE HAMBER Carry out the following steps to troubleshoot oxygen in the workspace chamber. 1. Make sure the arm port doors and sleeve assemblies are correctly installed. 2. Verify proper sleeve donning, and arm port entry and exit procedures have been used. 3.
OPERATION (CONTINUED) XITING THE HAMBER Withdrawing your arms through the arm ports creates a negative pressure in the chamber. If done too quickly, the motion can draw in outside oxygenated atmosphere through the sleeve cuffs or manometer. Use the following steps to exit the chamber without pulling in aerobic atmosphere. 1.
OPERATION (CONTINUED) ET THE EMPERATURE OINT Perform the steps below to change the set point to the operational temperature you will be using during your incubation application. Incubators come from the factory with a set point of 37°C. Each incubator set point must be set independently. Note: The visual example below depicts adjusting the incubator set point from 35°C to a 37°C application temperature.
OPERATION (CONTINUED) EMPERATURE CCURACY ERIFICATION Note: Performing a temperature accuracy verification requires a temperature reference device. Please see the Reference Sensor Device entry on page 12 for the device requirements. Optional: Each BACTRON incubator was calibrated at the factory at 37ºC. A verification of the display accuracy may be carried out when preparing the incubator for use or when required by your laboratory or production protocol.
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OPERATION (CONTINUED) Verifying the Temperature Display Accuracy 1. Once the chamber temperature has stabilized, compare the reference Reference Device temperature device and incubator display readings. a. If the readings are the same, or if a difference between the two falls within the acceptable range of your protocol, the display is accurately showing the chamber air temperature.
OPERATION (CONTINUED) Note: Test the OTL system at least once per year for functionality. Note: Each incubator OTL must be set independently in the BACTRON900. ET THE EMPERATURE IMIT The incubator must be operating at your incubation application temperature, and must be stable for at least 1 hour prior to setting the OTL.
OPERATION (CONTINUED) YCLING THE The pass box operates as an airlock, so at least one door must be closed at all times when an anaerobic atmosphere is present in the workspace chamber. If the main control panel Aerobic light is lit, the outer pass box door has been opened since the last auto cycle, allowing oxygenated atmosphere into the pass box.
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OPERATION (CONTINUED) Manual Cycle Users may manually cycle the pass box. This option is provided as a backup for the auto cycle. 1. Both pass box doors must be closed and latched, with the Doors light illuminated on the main control panel, to safely cycle the pass box. a.
OPERATION (CONTINUED) AMPLES Sheldon Manufacturing recommends waiting 24 hours after establishing an anaerobic atmosphere before loading samples into the unit. Airtight containers can introduce significant amounts of oxygen into the anaerobic environment of the BACTRON. Whenever possible, closed containers placed in the pass box should be loose- capped or ventilated to allow the pass box cycles to draw oxygen from the containers.
OPERATION (CONTINUED) EACTIVATING THE ATALYST ARTRIDGES Note: A flow of AMG for testing and cleaning can be obtained by disconnecting the foot pedal GAS - VAC line from an arm port while the door is closed, and then depressing the GAS pedal. An active catalyst cartridge must be installed in the workspace chamber to achieve and maintain anaerobic conditions.
OPERATION (CONTINUED) OLATILE OMPOUND CRUBBER AND EJUVENATION YCLE Activated carbon scrubber media can be placed in the workspace chamber to absorb volatile fatty acids (VFAs) or volatile sulfur compounds (VSCs) produced by sample cultivation. This helps keep the workstation interior clean, reduces odors, and prolongs catalyst endurance during cultivation processes producing significant amounts of VFAs or VSCs.
OPERATION (CONTINUED) EIONIZED AND ISTILLED ATER Do not use deionized water for cleaning or humidifying the BACTRON! While commonly available in laboratory environments, deionized water is an aggressive solvent that attacks most metal surfaces. Use of deionized water in a BACTRON voids the manufacturing defect warranty and may damage the workstation.
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USER MAINTENANCE Chamber Quality Control Check Sheet Month: Catalyst Condensate Monitoring Record Incubator AMG Cylinder Temperature Changed Receptacle Drained Strip Changed Pressure Reading Date: You may copy this sheet for institutional use 58 | P a g e...
USER MAINTENANCE (CONTINUED) AILY AINTENANCE 1. Exchange the catalyst cartridge in the chamber with a reactivated one. 2. Remove and empty the condensate collection container as needed. 3. Change the anaerobic monitoring strip at least once per 24 hours, or as often as necessary so that the strip in the workspace chamber remains moist.
USER MAINTENANCE (CONTINUED) – U IAGNOSTIC NIT IN Perform this procedure to check for leaks in and around the workspace chamber when the BACTRON is loaded with samples and cannot be taken out of use. Leaks can result from damage, long-term wear on BACTRON components, or from user error.
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USER MAINTENANCE (CONTINUED) Check the Arm Port Doors Failure to correctly close and seal the arm ports can result in the chamber leaking anaerobic atmosphere and increasing the rate of gas injections while sitting undisturbed. 10. Check the door ring seals for signs of damage or excessive wear. Replace the rings if there are obvious signs of damage or wear.
USER MAINTENANCE (CONTINUED) – E HECK MPTY Use this comprehensive procedure to verify the atmospheric integrity of the workspace chamber when the BACTRON can be taken out of operation. All samples should be removed from the workstation prior to carrying out this procedure as aerobic atmosphere will be present in the chamber.
USER MAINTENANCE (CONTINUED) 13. The pass box doors light should illuminate, indicating that both doors are closed. a. If the light does not activate, check that both doors are sealed. b. If the light does not activate and both doors are sealed, there may be a problem with door sensors.
USER MAINTENANCE (CONTINUED) Figure 32: Reassembling the EPLACING LEEVE SSEMBLY Sleeve Assembly OMPONENTS Replace any sleeve assembly component that shows signs of brittleness, dryness, or developing cracks. Typically the cuffs have the fastest rate of wear. Carry out the following steps to disassemble the sleeve assembly 1.
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USER MAINTENANCE (CONTINUED) Warning : Prior to maintenance or service on this unit, disconnect the power feed from the power supply. Avertissement : Avant d'effectuer toute maintenance ou entretien de cet appareil, débrancher le cordon secteur de la source d'alimentation. LEANING AND ISINFECTING If a hazardous material or substance has spilled in the unit, immediately initiate your site...
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USER MAINTENANCE (CONTINUED) Disinfecting Disinfect the BACTRON on a regular basis. Perform the following steps to carry out a complete disinfection of the workstation: 1. Turn the unit off. Open all doors and carry out your laboratory, clinical, or production space disinfection protocol.
Refer to the operation manual supplied with your vacuum pump for recommended maintenance routines. LECTRICAL OMPONENTS Electrical components do not require maintenance. If the BACTRON electrical systems fail to operate as specified, please contact your Shel Lab dealer, distributor, or Technical Support for assistance. 68 | P a g e...
80% relative humidity? If so, lower the room’s humidity. Does the number of media containers in the BACTRON exceed its rating? The BACTRON300 can hold 300 plates; the BACTRON600 holds 600 plates; the BACTRON900 can hold 900 plates. Reduce the number of sample containers. ...
USER MAINTENANCE (CONTINUED) ALIBRATE THE EMPERATURE DISPLAY Note: Performing a temperature display calibration requires a temperature reference device. Please see the Reference Sensor Device entry on page 12 for device requirements. Temperature calibrations are performed to match an incubator temperature display to the actual air temperature inside the incubator.
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USER MAINTENANCE (CONTINUED) Temperature Calibration Reference Device 1. Once the incubator temperature has stabilized, compare the reference device and incubator temperature display readings. a. If the readings are the same, or the difference between the two (2) falls within the acceptable range of your protocol, the display is accurately showing the test space chamber air temperature.
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USER MAINTENANCE (CONTINUED) Temperature Calibration (Continued) Reference Device 4. Use the Up or Down arrows to adjust the current display temperature value until it matches the reference device temperature reading. 5. After matching the display to the reference device, wait 5 seconds. a.
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USER MAINTENANCE (CONTINUED) Temperature Calibration (Continued) Reference Device 8. If the two readings are not the same, and the difference still falls outside the acceptable range of your protocol, repeat steps 3 – 7 up to two more times. a. Three calibration attempts may be required to successfully calibrate units that are more than ±2°C out of calibration.
We reserve the right to alter technical specifications at all times. EIGHT Model Shipping Weight Unit Weight 610lbs / 276kg 328.5lbs / 149kg BACTRON300 760lbs / 344.7kg 412lbs / 187kg BACTRON600 760lbs / 344.7kg 436.5lbs / 198kg BACTRON900 ORKSTATION...
TAND IMENSIONS Model Inches W × D × H Centimeters W × D × H BACTRON300 61.5 x 30 x 30 inches 156 x 76.2 x 76.2 cm BACTRON600 88.5 x 30 x 29 inches 225 x 76.2 x 74 cm BACTRON900 88.5 x 30 x 29 inches...
UNIT SPECIFICATIONS (CONTINUED) OTAL LATE APACITY Model Plates BACTRON300 BACTRON600 BACTRON900 EMPERATURE Uniformity Uniformity Model Range Workspace Incubator Side Incubator BACTRON300 Ambient +5C to 70C ±-0.5C @ 37C ±1C @ 37C BACTRON600 Ambient +5C to 70C ±1C @ 37C BACTRON900 Ambient +5C to 70C...
PARTS LIST Description Parts Number Description Parts Number Anaerobic Monitoring Strips Shelf Spacer (BACTRON300 and 900 workspace Incubator) (Box of 100 packets) 9900706 5680502 Pass Box Door Gasket 12 x 12 Foot Pedal Controls Assembly (burgundy) 3450507 9990735 Arm Port Door Left Fuse, Power Cord Inlet, Type T 12.5Amp,...
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PARTS (CONTINUED) Description Parts Number Description Parts Number Sleeve Cuffs Latex, Size 7 Sleeve Assembly Size 6.5, Extra Small (2 cuffs, 2 cuff-rings, 4 0-rings, 2 (for sleeve assembly) sleeves, 2 self-gripping straps) 9990738XS Sleeve Cuffs Latex, Size 8 Sleeve Assembly Size 7, Small (2 cuffs, 2 cuff-rings, 4 0-rings, 2 sleeves, (for sleeve assembly) 2 self-gripping straps)
PARTS (CONTINUED) RDERING ARTS AND ONSUMABLES If you have the Part Number for an item, you may order it directly from Sheldon Manufacturing by calling 1-800-322-4897 extension 3. If you are uncertain that you have the correct Part Number, or if you need that specific item, please contact Sheldon Technical Support for help at 1-800-322-4897 extension 4 or (503) 640-3000.
ACCESSORIES Shel Lab offers the following BACTRON accessories for sale. Please see our website for prices. Activated Carbon Media (2 lbs / 0.9 kgs) For scrubbing hydrogen sulfides, fatty acids, and some toxic or corrosive compounds from the workstation atmosphere.
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Microscope. BACTRON300: Part Number 9990535 BACTRON600 and BACTRON900: Part Number 9990511 BACTRON300 Stand A rolling stand with cabinet for the BACTRON300. 29.3 inches high by 61.5 inches wide (74cm high by 156cm wide) Part Number 9000511 BACTRON600/900 Stand A rolling stand with two (2) cabinets for the BACTRON600 and 900.
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ACCESSORIES (CONTINUED) Leak Detector A handheld gas detector for locating leaks. Recommended for units that have been in service for 4 or more years. Part Number 4600501 Leica S6 Spotting Stereo Microscope and Assembly Requires the appropriate BACTRON microscope adaptor. Part Number 9990516 Lukas Fiber Optic Micro Lite Illumination System A fiber optic, adjustable brightness, halogen light box and guide.
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ACCESSORIES (CONTINUED) UV Viewing Lamp A handheld UV lamp for use with BACTRON workstations. Parts Number 9490507 Zephiran Benzalkonium Chloride Chamber Cleaner 1 Gallon, 0.133%. Part Number 1060501 84 | P a g e...
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