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Summary of Contents for Shel lab BACTROX
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Page 1: Operation Manual
BACTROX HYPOXIC CHAMBERS OPERATION MANUAL MODEL: Bactrox Sheldon Manufacturing Inc. P.O. Box 627 Cornelius, Oregon 97113 EMAIL: tech@Shellab.com INTERNET: http://www.Shellab.com 1-800-322-4897 (503) 640-3000 FAX (503) 640-1366 Rev 9/11/12 4861671... -
Page 2: Table Of Contents
THERMOELECTRIC CONDENSATE CONTROLER ............ 9 INCUBATOR TEMPERATURE CONTROLLER/SET OVER TEMPERATURE .... 9 CO2 CONTROL CALIBRATION .................. 10 OXYGEN CONTROL CALIBRATION ................11 POWER ON THE BACTROX: ..................13 MAKING THE CHAMBER HYPOXIC ................14 PASS BOX OPERATION ..................... 14 ENTERING THE CHAMBER ..................15 EXITING THE CHAMBER .................... -
Page 3: Orientation
NOTE: This equipment must be used only for its intended application; any alterations or modifications will void your warranty. 1. ORIENTATION Control Panel Document/Chart holder Connections Incubator Oxygen Sensor & Pass Box Arm Port Door Arm Ports Storage With Sleeve Bactrox Operations Manual... - Page 4 Figure 2: Arm port Doors CAUTION: The Bactrox chambers have an efficient method of sealing the chamber by utilizing the Arm port doors as shown in Figure 3. The tightening knob on the Arm port doors should be turned just until the knob begins to “grab”.
- Page 5 The PASS BOX shelf allows for materials to be conveniently rolled from the PASS BOX into the chamber. Figure 6: Incubator Bottom Shelf Spacer Three (3) are provided with the Bactrox. Refer to Section 8 for installation and precautions. Bactrox Operations Manual...
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Page 6: Receiving And Inspection
A typical cleaning procedure is described below and will help reduce the likelihood of contamination and the necessity of decontamination. Clean the unit regularly. Depending on usage and protocol, this may be weekly, monthly or quarterly. Cleaning the unit does not qualify the unit as decontaminated. Bactrox Operations Manual... - Page 7 Special care should be taken when cleaning around sensing heads to prevent damage. The Bactrox unit was cleaned at the factory, however, a general cleaning is recommended. Use BENZALKONIUM CHLORIDE to clean your chamber. See list of chamber accessories products. Do not use chlorine-based bleaches, ammonium-based disinfectants or aerosol canisters or abrasive cleaners.
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Page 8: Control Panel Overview
The high pressure gauge (0-4000 PSI) will indicate the pressure within the tank. The low pressure gauge (0-60 PSI) will indicate the output pressure on the supply hose to the chamber. Single stage regulators do not provide as stable performance as dual stage. Bactrox Operations Manual... -
Page 9: Thermoelectric Condensate Controler
10 PSI to prevent overshoot. Hose ports for the gas are provided on the upper left side of the unit. The Bactrox chamber has five access ports on the left side of the unit. See Diagrams Section page 33 (INTERIOR PLUMBING) for more details. -
Page 10: Co2 Control Calibration
OFF. Adjusting CO Display: After the Bactrox has had several hours to stabilize at the desired CO set point and measure the actual CO % with a Fyrite gas analyzer. If there is any difference... -
Page 11: Oxygen Control Calibration
Offset calibration can be done through front panel display buttons. See the OFFSET Calibration section below. To complete a two point linear calibration the Bactrox needs to be hooked up to a computer that has RS-232 capability with windows terminal or internet access to download TERATERM off the web. - Page 12 O will have a negative impact. 9.5.5 Turn on the Bactrox and allow 5 minutes for the oxygen sensor to warm up. The oxygen sensor needs to warm up before it can start reading oxygen levels accurately.
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Page 13: Power On The Bactrox
9.6.10 Remove the calibration gas and calibration cover. It is important to note that a single point calibration may cause O readings outside of the calibration gas to be incorrect. POWER ON THE BACTROX: 10.1 Check power supply against unit plate and make sure they match. Be certain that the fuse is installed in the power inlet of the unit. -
Page 14: Making The Chamber Hypoxic
Put arm port doors in place, or the Arm Port Sleeve Plugs NOTE: The Bactrox chambers have an efficient method for sealing the chamber by utilizing the Arm port doors as shown in Figure 3. The tightening knob on the Arm port doors should be snug, DO NOT OVER TIGHTEN. -
Page 15: Entering The Chamber
Anatox-1000 grams (packaged in 250 gram packages) Instructions: Empty two (2) 250 gram packages into two (2) separate 500 ml Pyrex beakers. Day one – place one beaker in the chamber (new Anatox can be placed directly into the chamber). Bactrox Operations Manual... -
Page 16: Accessories
Tank Switch Chamber Stand Switches and monitors dual gas tank supply. Provides Provides work bench to support Bactrox chambers. Chamber Stand height 30”. Stands feature locking automatic switch-over to second gas tank alarm sounds when both tanks are empty. Tanks can be casters and storage space. -
Page 17: Troubleshooting
Call for service. III. MISCELLANEOUS Problem Possible Cause Solution Role of Condensate Controller: The Condensate Controller is an exclusive Bactrox feature of Sheldon Manufacturing, Inc. Our competitors use desiccants. Desiccants are a drying agent and often dry out samples. Bactrox Operations Manual... - Page 18 B2. Cover chamber with a blanket overnight as means of installation. C. Air conditioner unit directly C. Relocate chamber, redirect above chamber. the air conditioning duct. D. Thermoelectric condensate D. Empty thermoelectric controller not emptied condensate controller regularly. container. Bactrox Operations Manual...
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Page 19: Bactrox Operation Tips
The location that the cuffs seal on the user’s arm is determined by individual preference. The size of the cuffs that come standard with the Bactrox Chamber is medium, size 8 glove. Other sizes are available; large size 9, and small size 6 ½. -
Page 20: Unit Specifications
Temperature Range Temperature O2 Range CO2 Range (Control) Uniformity BACTROX, Ambient + 8 to 60C +/- 0.35C @ 37C 0-20% 0-20% BACTROX-2 (+/- 0.1C) Electrical Model Volts Amps Cycles BACTROX 1100 50/60 hz BACTROX-2 1100 50/60 hz Bactrox Operations Manual... -
Page 21: Diagrams
__________________________________________________________________________ DIAGRAMS WIRING DIAGRAM BACTROX (9851358) BLACK 14G EMI FILTER INLET 2800502 4200505 WHITE 14G BLACK 14G WHITE 14G GREEN LIGHTED POWER SWITCH 7850570 WHITE 14G BLACK 14G CHAMBER FAN 4880550 TB4#19 TB1#1 BLACK 14G WHITE 14G TB4#23 TB1#3 AIR PUMP... - Page 22 __________________________________________________________________________ WIRING DIAGRAM BACTROX-2 (9851363) BLACK 14G EMI FILTER INLET 2800502 4200505 WHITE 14G WHITE 14G BLACK 14G WHITE 14G GREEN LIGHTED POWER SWITCH 7850570 WHITE 14G BLACK 14G CHAMBER FAN 4880551 TB4#19 TB1#1 BLACK 14G WHITE 14G TB4#23 TB1#3...
- Page 23 __________________________________________________________________________ BACK PANEL BACTROX (9851359) TEMPERATURE TTB1 SENSOR BROWN 24G BLACK 24G 8000547 BLACK 24G WHITE 24G RED 24G BLACK 24G 4 LOOP CONTROL BOARD SL# 1750972 24VDC POWER 12VDC SUPPLY 6750516 POWER SUPPLY 6750514 8000542 TB5-1 TB5-2 TB5-3 TB5-4...
- Page 24 __________________________________________________________________________ BACK PANEL BACTROX-2 (9851364) TEMPERATURE TTB1 SENSOR BROWN 24G BLACK 24G 8000547 BLACK 24G WHITE 24G RED 24G BLACK 24G 4 LOOP CONTROL BOARD SL# 1750972 24VDC POWER 12VDC SUPPLY 6750516 POWER SUPPLY 6750514 8000542 TB5-1 TB5-2 TB5-3 TB5-4...
- Page 25 __________________________________________________________________________ CONTROL PANEL BACTROX (9851360) Bactrox Operations Manual...
- Page 26 __________________________________________________________________________ CONTROL PANEL BACTROX-2 (9851365) WHITE BLACK GREY GREY BLACK WHITE BLACK WHITE RED HT ORANGE BLACK 18G RED 18G Bactrox Operations Manual...
- Page 27 __________________________________________________________________________ INCUBATOR & FAN BACTROX (9851361) FAN PIGTAIL CORD 1800536 120V 65W 2350502 CHAMBER FAN 4880550 120V 65W 2350502 INCUBATOR Bactrox Operations Manual...
- Page 28 __________________________________________________________________________ INCUBATOR & FAN BACTROX-2 (9851366) FAN PIGTAIL CORD 1800536 120V 65W 2350502 CHAMBER FAN 4880551 120V 65W 2350502 INCUBATOR Bactrox Operations Manual...
- Page 29 __________________________________________________________________________ CHILLER & OUTLET BACTROX (9851362) ALL END STRIP .375 AND SOLIDER TIN Bactrox Operations Manual...
- Page 30 __________________________________________________________________________ CHILLER & OUTLET BACTROX-2 (9851367) ALL END STRIP .375 AND SOLIDER TIN Bactrox Operations Manual...
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Page 31: Plumbing Diagram
__________________________________________________________________________ PLUMBING DIAGRAM SET 10 PSI SET 10 PSI REGULATORS 7150510 Bactrox Operations Manual... -
Page 32: Bactrox Spare Parts List
__________________________________________________________________________ BACTROX SPARE PARTS LIST DESCRIPTION PART NUMBER PART NUMBER QUANTITY 115 VOLT 220 VOLT CHECK VALVE FILTER 8600573 8600573 CONTROL - OTL SAFETY 1750500 1750500 CONTROL - PLC 1750621 1750621 CONTROL - TEMPERATURE CONTROL PROBE - OTP SAFETY 8100500...
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