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Takara Bio Thermal Cycler Dice Real Time System III Instrument Manual

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Thermal Cycler Dice
TM
Real Time System III
TP950/TP970/TP980/TP990
Model
LCD application software
V 1.00
Real Time System Software V 6.00
OS： Windows 7 / 8.1
Instrument Manual
TAKARA BIO INC.
TM
Attention: Thermal Cycler Dice
Real Time System Instrument Manual
should be stored for reference in a secure and accessible location.

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Summary of Contents for Takara Bio Thermal Cycler Dice Real Time System III

  • Page 1 LCD application software V 1.00 Real Time System Software V 6.00 OS： Windows 7 / 8.1 Instrument Manual TAKARA BIO INC. Attention: Thermal Cycler Dice Real Time System Instrument Manual should be stored for reference in a secure and accessible location.
  • Page 2 Thermal Cycler Dice Real Time System III (Cy5) with PC <Software of TP951 main body >  Thermal Cycler Dice Real Time System III LCD application software version 1.00  Thermal Cycler Dice Real Time System III Software version 6.00 for PC （Cat.# TP952）...

  • Page 3: Table Of Contents

    TP950 Instrument Manual ＝ Contents ＝ page Precautions for Using TaKaRa Scientific Instruments -- 2 Chapter 1: Outline -- 7 Chapter 2: Installation Requirements and Maintenance -- 10 1. Installation Conditions -- 10 2. Opening the Packaging -- 11 3. Installation -- 13 4.
  • Page 4 Precautions for Using TaKaRa Scientific Instruments ● Users are recommended to carefully read the content of this manual before operating the TaKaRa PCR Thermal Cycler Dice system. ● After familiarizing themselves with the information in this manual, users are recommended to store it in secure and convenient location from which it is always available for reference Introduction All Takara scientific instruments are sold strictly for use in research applications and should not be...
  • Page 5 （２）General Precautions Regarding Takara Scientific Instruments It is important to observe the various precautions described below to ensure optimum system performance and prevent accidents before they happen. Ensure safe and correct system operation by carefully reading the precautions. 【Precautions for Installation Location】 Observe the following precautions in order to prevent fires and electrical shocks.
  • Page 6 ● Be sure to compare system power supply requirements with the rated ！ WARNING power capacity of the power source to ensure system current requirements do not exceed power socket capacity. Confirm that the total power requirements of all connected equipment do not exceed the rated power supply capacity of the common power source.
  • Page 7 Please be sure to use 0.1mL-type for the reaction tube and the reaction plate used by this equipment. When 0.2mL type is inserted by mistake, it can't be taken any more out. Please use consumables of Takara bio recommendation. Caution Note...
  • Page 8 Precautions against Transferring or Copying Content of This Document The content of this document cannot be transferred or copied without the express permission of the copyright holder. Copyright holder: TAKARA BIO INC. Printed in Japan Contact TAKARA BIO INC. TAKARA BIO INC.
  • Page 9 TP950 Instrument Manual ● Chapter 1: Outline ™ Thank you for purchasing the Thermal Cycler Dice Real Time System III. This system consists of the following components: ™ <TP950> Thermal Cycler Dice Real Time System III ™ 1. Thermal Cycler Dice Real Time System III Main Unit (Code: TP951) ™...
  • Page 10 TP950 Instrument Manual System construction table System code TP950 TP970 TP980 TP990 Unit code System name Thermal Thermal Thermal Thermal Cycler Dice Cycler Dice Cycler Dice Cycler Dice Real Time Real Time Real Time Real Time System III System III System III System III with PC...
  • Page 11 TP950 Instrument Manual ™ The main features of the Thermal Cycler Dice Real Time System III are as follows: 【 TP950 】  The 96-well reaction block of aluminum with high power peltier units is use. Using the 96-well plate [0.1mL], 8-tube strip [0.1mL] or single tube [0.1mL]. ...

  • Page 12: Installation Conditions

    TP950 Instrument Manual ● Chapter 2: Installation Requirements and Maintenance 1. Installation Conditions To ensure optimum performance and long and stable product life, observe the following precautions when selecting an installation location. Note that installing the system in a location that does not meet the requirements listed below can result in malfunctions and/or system breakdown.

  • Page 13: Opening The Packaging

    TP950 Instrument Manual 2. Opening the Packaging This equipment comes packaged in carton, as shown below. The power cord is bundled at the front of the carton box. The gross weight with packaging is 21.5 kg, and the equipment itself weighs 16.1 kg. Take sufficient care when removing the equipment from the packaging. When lifting the main unit, please grip at the depressed parts at the bottom of the front panel and the back surface.
  • Page 14 ◆ Main Unit （Code: TP951） ◆ Power cord AC125V 15A 3m ◆ Software CD (Code: TP952) Thermal Cycler Dice Real Time System III Software & FEI Software ◇ Instrument manual and Quick manual ◇ Warranty card ◇ Consumable Accessory Pack 0.1ml 96 well plate for Real Time...

  • Page 15: Installation

    TP950 Instrument Manual 3. Installation Use the following procedures when installing system. Confirm that the system power switch is set to off. Connect the system side of the power cord to the power connector on the rear of the system. Plug the AC power plug of the cord into a power outlet.
  • Page 16 TP950 Instrument Manual Turn on the power of a control computer. An icon on the desktop of a control computer is double-clicked and software is started. When message of "Instrument: Connected" and "Camera: Connected" went out to the lower right of a screen, a connection of the body and a control computer normally connected.

  • Page 17: Moving The System

    TP950 Instrument Manual 4. Moving the System Follow the procedures described below when moving the system to a new location after it has been installed. ● New Location within the Same Site Follow the precautions listed below when moving the system to a new location within the same site.

  • Page 18: Part Names And Functions

    TP950 Instrument Manual ● Chapter 3: Part Names and Functions Exterior (Lid Closed) ①Lid Handle ⑤LCD Display ⑨Power Lamp ⑥Power Button ⑦Ventilation intake ⑩USB Port Rev. J...
  • Page 19 TP950 Instrument Manual Front (Lid Open) ⑧Lid Lock Catch ①Lid Handle ②Lid ③Sample Block ⑤LCD Display ⑨Power Lamp ⑥Power Button ⑦Ventilation intake ④Lid Lock ⑩USB Port Explanation ――――――――――――――――――――――――――――――――――― ① Lid Handle To open the Lid, lift the Lid Handle, slide the Lid towards the back and push it into the main unit.
  • Page 20 TP950 Instrument Manual ④ Lid Lock While the program is in operation, the Lid becomes locked so that itcan not be opend. ⑤ LCD Display Display and input for the Real Time System LCD software. 【Caution】 The liquid crystal panel is damaged for glassware when you add a strong shock.
  • Page 21 TP950 Instrument Manual Back ⑬LAN Port ⑪Main Power Switch ON（｜）、OFF（○） ⑦Ventilation Exhaust ⑫Power Cord Connector ⑭Circuit Protector Explanation ――――――――――――――――――――――――――――――――――― ⑪ Main Power Switch Switch for turning the main unit power to ON（｜） or OFF（○）. ⑫ Power Cord Connector Connector for Power cord. 【Warning】...
  • Page 22 TP950 Instrument Manual LCD Display （Home Screen） “Run” status Shut Down Button “Stand by” status Communication Selector File Name “Lid” status Lid Temperature Shut Down Button Shut down the Real Time System Software. and stand by state. “Stand by” status ⇔...

  • Page 23: Explanation Of Screens

    TP950 Instrument Manual ● Chapter 4: Explanation of Screens 1. System Startup and Shut Down (1) Startup ® ① Turn on the main power switch in the Thermal Cycler Dice Real Time System III body back. ® ② The power button on the front left side of Thermal Cycler Dice Real Time System is pressed.
  • Page 24 TP950 Instrument Manual Refer to "Software Operation Manual" for using method of software. （２）Shut down ① Click on the “Shut Down” button across the top of the home screen ® of Thermal Cycler Dice Real Time System. 【Caution】 The dialogue, asking about the advisability of the preservation is indicated about Experiment File of non-preservation.

  • Page 25: Home Screen

    TP950 Instrument Manual ２．Home Screen ® When having started the Thermal Cycler Dice Real Time System, it's indicated first. “Stand by” status Communication Shut Down Button Selector “Run” status ボタン “Lid” status File Name Lid Temperature リッド温度 ■Home Screen Experiment Create the new Experiment File.

  • Page 26: Self-Diagnostic Function

    TP950 Instrument Manual ● Chapter 5: Maintenance 1. Self-diagnostic Function If the PCR reaction does not proceed well, it possible to determine whether the temperature control for the main equipment is operating normally using the self-diagnostic function for Sample Block temperature efficiency. In addition, the status of the temperature control function can be tracked by performing periodic self-diagnostic tests.
  • Page 27 TP950 Instrument Manual The Self-Diagnosis Screen is displayed. Select the diagnostic items, and touch on Start button. The first step of the diagnostic program starts. When continue the next diagnostic, touch on Stop button once. Making sure appeared “Stand by”, touch on Start button.
  • Page 28 TP950 Instrument Manual ① Rate Test Program Execution Screen (Refer to the previous figure.) Program Contents: 25°C/30 seconds, 94°C/30 seconds, 40°C/30 seconds Specification:  Maximum heating rate greater than or equal to 4.5°C per second  Maximum cooling rate greater than or equal to 3.0°C per second ②...

  • Page 29: Daily Inspection

    TP950 Instrument Manual 2. Daily Inspection Perform the following inspections and cleaning periodically in order to maintain theoperational performance of the equipment. Every time  When a solvent, wax and cosmetics, etc. were stuck in the body surface, it's wiped up promptly. * A crack may occur to plastic resin.

  • Page 30: Sample Block Cleaning

    TP950 Instrument Manual 《Sample Block Cleaning》 Clean the Sample Block once-monthly, occasionally based on frequency of use or soiling conditions. If the Sample Block is duty, it may affect the strength of the fluorescence. In order to check whether the Sample Block is soiled/dirty, tighten the Lid without putting anything on theSample Block, and then photograph in FAM and ROX modes and verify that fluorescence is not visible.
  • Page 31 TP950 Instrument Manual ③ Click TakePicture button on the Optical CalibrationScreen. Set the fluorescent dye to FAM with Expo=400, Gain=4. Set “FAM” dye. Set Expo 「400」 Touch the area of “Expo「200」” ,then appear the And Gain 「4」. keyboard. Input 「400」 and touch button.
  • Page 32 TP950 Instrument Manual ⑥ If the Sample Block is soiled, soak a cotton slender swab ethanol and wipe off any accumulated dirt. 【 Note 】 When find any dirt, try to soak the cotton slender swab with tap water. Clean wells. ⑦...

  • Page 33: Other Settings

    TP950 Instrument Manual ● Chapter 6: Other Settings In other setting "Other Setting", (1)Ethernet Setup, (2)Brightness, (3)Beep, (4)Mode, (5)Date & Time. The respective functions are explained in the next. １．Enter “Other setting”. Select “Instrument” from Home screen and click “Other Setting”. Rev.

  • Page 34: Explanation Of Each Function

    TP950 Instrument Manual ２．Explanation of each function （１） Ethernet Setup IP Address： An IP address is designated. Factory setting 192.168.0.10 Subnet Mask： A subnet mask is designated. Factory setting 255.255.255.0 Operation： ・Local- The unit is controlled by itself. Selected “Local” mode can't be connected to the unit from the outside control PC.
  • Page 35 TP950 Instrument Manual （３） Beep Select “On” or “Off“ for the Beep sound when touch the LCD panel. Click OK button. Default setting is “On”. （４） Mode Setting the functions ( Language, File Type, Default Speed ) when starting the unit. Language：...
  • Page 36 TP950 Instrument Manual ・Fast- 【Default setting】 Fast speed mode has maximum heat rate and cool rate in PCR cycle. ・Normal- Normal speed mode has TP800 compatibility that heat rate and cool rate are controlled temperature in PCR cycle. （５） Date & Time Set the date (Year/Month/Day) and time (Hour: minute: second).
  • Page 37 TP950 Instrument Manual ● Chapter 7: Troubleshooting Symptom Cause Correspondence Main Unit (TP951) -1 Nothing happens when the Power is not being supplied. Verify the Power Cord connection on Power Switch is turned ON. the main unit side and the power outlet The Power Cord is not securely side plugged in to the power outlet or...
  • Page 38 TP950 Instrument Manual Symptom Cause Correspondence Main Unit (TP951) -2 The PCR program does not Warm-up for the Lid heater has Try again after allowing 103℃ over of start. not yet finished to warm up. Lid heater temperature. The heater or a safety fuse has A part needs to be replaced.
  • Page 39 TP950 Instrument Manual Symptom Cause Symptom Main Unit (TP951) -3 The fluorescence Filter unit isn't set right. Set filter unit right. measurement value (graph) Abnormality of the filter change A replace of the filter change unit or a is abnormal. unit.
  • Page 40 TP950 Instrument Manual Symptom Cause Correspondence Main unit (TP951) and Control PC (TP820) The Main unit and a A LAN cable isn't connected by Connect a LAN cable by the cross computer don't connect. the cross type. type of the accessory. It doesn't (LAN communication of the connect by the straight type.

  • Page 41: Computer Settings

    TP950 Instrument Manual Computer settings i) Clic on the right button in the Disk Top. ii) Select “Personalize” and clic. iii) Clic “Screen Saver”. vii) Confirm the following settings. iv) Select “(None)” on Screen Saver, and then clic “Change power settings”. v) Select “High performance”...

  • Page 42: Specifications

    TP950 Instrument Manual ● Chapter 8: Specifications Main Unit Basic Specifications ® Product Name Thermal Cycler Dice Real Time System III main unit Part Number TP951 96-Well Plate ( Sealing film type ) Objects Processed 8- trip tube ( Sealing cap type) Optical Detection CCD Camera ( 1280 x 960 pixel ) System...
  • Page 43 TP950 Instrument Manual Battery Lithium Cell Battery Type: CR2032, 3V Connection with PC Ethernet, LAN cable (Cross type) Supply Voltage AC 100V～240V, 50/60 Hz Maximum Current 7．5 A ( at AC100V ) Power Cord 125 V 15 A cable (3 m) including a 3-pin plug connection with a ground pin (commonly used in Japan and North America) * Use a proper power cord when supply line voltage is more than 125V.
  • Page 44 TP950 Instrument Manual ● Chapter 9: Consumption ■ 0.1mL 96well plate Code Product Quantity Comments NJ900 0.1ml 96 well plate for Real Time 10 plates NJ500 Sealing Film for Real Time 100 seats Pressure-sensitive adhesive NJ501 Sealing Film for Real Time 100 seats Adhesive film (Adhesive)
  • Page 45 TP950 Instrument Manual Appendix Code TP952 Thermal Cycler Dice Real Time System Software for TP950 Install / Uninstall  If the Thermal Cycler Dice Real Time System Software is being installed for the first time, perform the steps described in section 1 (Install). ...
  • Page 46 TP950 Instrument Manual ☆☆ Windows 7 （English） ☆☆ Software Install / Uninstall A． 1. Install ① Insert the Thermal Cycler Dice Real Time System Software CD in to the CD drive. ② Make sure the OS is Windows 7 English version. Double-click on the English folder in the CD drive.
  • Page 47 TP950 Instrument Manual ⑥ Click the “Next” button in the screen below. The installation process begins. ⑦ The Installation Execution screen is displayed. On the way, when “UAC” appear click “Yes”. ⑧ When installation is complete, the right screen is displayed. Click “Close”...
  • Page 48 TP950 Instrument Manual 2. Uninstall ① From the Start menu, go to Control Panel and double-click on the Programs icon. View by Category View by Small icons ② When the “Uninstall or Change a Program” window is displayed, select “Thermal Cycler Dice Real Time System Software”...
  • Page 49 TP950 Instrument Manual ⑤ When complete, a dialog box inquiring whether to restart now or later is displayed. If additional software is to be installed, select “No”. The following screen is displayed. After verifying that the Thermal Cycler Dice Real Time System program has been removed, click Close.
  • Page 50 TP950 Instrument Manual ☆☆ Windows 8.1 （English） ☆☆ Software Install / Uninstall B． 1. Install ① Insert the Thermal Cycler Dice Real Time System Software CD in to the CD drive. ② Make sure the OS is Windows 7 English version. Double-click on the English folder in the CD drive.
  • Page 51 TP950 Instrument Manual ⑥ Click the “Next” button in the screen below. installation process begins. ⑦ The Installation Execution screen is displayed. On the way, when “UAC” appear click “Yes”. ⑧ When installation is complete, the right screen is displayed. Click “Close”...
  • Page 52 TP950 Instrument Manual 2. Uninstall ① From the Start screen or the Desktop screen, click the right button on the start icon. The menu appears. Select the “Programs and Features” for uninstall. When the “Uninstall or Change a ② Program” window is displayed, select “Thermal Cycler Dice Real Time System Software ”...
  • Page 53 TP950 Instrument Manual ④ When un-installation is complete, a dialog box inquiring whether to restart now or later is displayed. Click “Yes” for reboot. Rev. J App(1) - 9...
  • Page 54 TAKARA BIO INC. NOJI HIGASHI 7-4-38, KUSATSU, SHIGA, 525-0058 URL: http://www.takara-bio.com 2015.11...

This manual is also suitable for:

Tp950Tp970Tp980Tp990

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