NanoEnTek JuLI Stage User Manual
  1. Manuals
  2. Brands
  3. NanoEnTek Manuals
  4. Voice Recorder
  5. JuLi Stage
  6. User manual

NanoEnTek JuLI Stage User Manual

Real-time cell history recorder
Hide thumbs Also See for JuLI Stage:
Table of Contents

Advertisement

Quick Links

Download this manual
Single-cell Observation System for C1 IFC Vessel
User Manual
Copyright
NanoEnTek Inc. All rights reserved.
TM
JuLI
Stage, Real-Time Cell History Recorder ©2017 NanoEnTek Inc.
1

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the JuLI Stage and is the answer not in the manual?

Questions and answers

Subscribe to Our Youtube Channel

Related Manuals for NanoEnTek JuLI Stage

Summary of Contents for NanoEnTek JuLI Stage

  • Page 1 Single-cell Observation System for C1 IFC Vessel User Manual Copyright NanoEnTek Inc. All rights reserved. JuLI Stage, Real-Time Cell History Recorder ©2017 NanoEnTek Inc.
  • Page 2 The information in this manual is described as accurately as possible. The changes involved in firmware and software may be applied without notification. Copyright ©2017 by NanoEnTek Inc. All rights reserved. Published in Korea. Documentation: NESMU-JSTSC-001E Revision history: V.0.0 Date: JAN 2017 V.0.1 Date: JUN 2017...

  • Page 3: Table Of Contents

    Table of Contents Safety Precautions 42-43 Product components Product specifications Product overview Ordering information 45-46 Features Technical support Description -Front view -Side view -Rear view -Control box Installation 10-13 - Unpacking the instrument - Remove the filter change lock module - Install the instrument - Place the instrument - Operating environment...
  • Page 4 Please check all components listed below when the instrument is delivered. PC will be shipped separately because it is optional. If any item is missed of damaged, contact your local distributor or e-mail sales@nanoentek.com. USB Cables (20 ~ 43 cm)
  • Page 5 Product overview C1 IFC vessel imaging using JuLI Stage JuLI Stage Single-cell Observation System (SOS) enable imaging and analysis of single cells in C1 integrated fl uidic circuits (IFC) vessel. C1 IFC vessel allow capture and stain single cells for further analysis i.e RNA, DNA extraction and gene sequencing like NGS. JuLI Stage Single-cell Observation System (SOS) is composed of software controls the automated X-Y axis stage and features imaging and analysis tools and compact, compatible system, JuLI...
  • Page 6 - Compact and compatible to CO incubator - 23.8” full HD monitor - Desktop computor - Simple steps for the system setup and operations - Compatibility with various brands of well plates by auto adjustment function JuLI Stage, Real-Time Cell History Recorder ©2017 NanoEnTek Inc.

  • Page 7: Description

    Description Cover Used to protect test samples from external light. When you use the Front JuLl™ Stage in an incubator, the cover can block out light from the View Front view of the JuLl™Stage. The main body is composed of LED, Main body Filters (Fluorescence mode) and optic parts.

  • Page 8: Rear View

    Serial port Control the camera in the JuLI™ Stage main instrument. Camera port Control the JuLI™ Stage main instrument operating. Serial connection LED Camera connection LED Serial port Camera port Connection port JuLI Stage, Real-Time Cell History Recorder ©2017 NanoEnTek Inc.

  • Page 9: Installation

    Installation Unpacking 1. Open the box and remove the material foam in the box. 2. Carefully lift the instrument out of the box, holding it by handholds in the instrument the bottom of the base. 3. Place the instrument on a fl at, level surface (table or incubator). IMPORTANT! Do not lift the JuLI™...

  • Page 10: Install The Instrument

    3. Plug the power cord of PC and instrument into the electrical outlet. ☞ Note: Be sure to use only the power cord supplied with your instrument. Powering the instrument with an unapproved power cord may damage the instrument. JuLI Stage, Real-Time Cell History Recorder ©2017 NanoEnTek Inc.

  • Page 11: Place The Instrument

    Installation Live-cell images from various cell culture dishes are directly captured in Place the a cell culture incubator. This design allows an instrument to be installed instrument in an incubator, and prevents the contamination by maintaining a sterile into the environment.

  • Page 12: Operating Environment

    • Place the JuLI™ Stage on a level surface away from vibrations from other pieces of equipment. • Operating temperature range: 5 - 40°C (41 - 104°F) • Relative humidity range: 20 - 95 %. JuLI Stage, Real-Time Cell History Recorder ©2017 NanoEnTek Inc.

  • Page 13: Basic Operaion

    Basic Operation Make sure the instrument, control box, and PC are plugged IMPORTANT! Start the in and connected. Single Cell If the system is not setup correctly, contact FAS. For contact information, see page Observation You must start the components of the Single-cell Operation System Software (Instrument, PC and Software) in the following order so as the software can connect to the instrument...

  • Page 14: Insert The C1 Ifc Vessel Into The Holder

    Notch (A1 position of C1 IFC vessel) C1 IFC vessel into the holder Be ensure that the notch (A1 position) is set to top-left corner of vessel and the barcode faces to the left side. JuLI Stage, Real-Time Cell History Recorder ©2017 NanoEnTek Inc.

  • Page 15: Software Activation

    Basic Operation Software should be licensed by activation key in setting menu Software • Popup window inform how to receive license key Activation ☞ NOTE: Preview and Option tab is deactivated until software activation Click Activation and input authorized 20 character license key to activate software Single-cell Observation S...

  • Page 16: Windows Update

    Please do not allow the windows update. Windows update ☞ NOTE: Do not connect the internet network. We recommend you to allowe the network connection only for remote access and monitoring. JuLI Stage, Real-Time Cell History Recorder ©2017 NanoEnTek Inc.

  • Page 17: Components Of The Software

    Components of the Software IFC display Image tab Scan tab Analysis tab Setting tab IFC location Live image pane 1. Live image pane – Displays an image of the selected IFC location 2. IFC location – Displays the current IFC location as <Site> I <Column> x <Row> 3.

  • Page 18: Before Start

    • To regulate focus, drag focus slide or click the focus buttons (Coarse and Fine) • Coarse focus interface – Larger step which moves up or down rapidly • Fine focus interface – Smaller step which moves short distances minutely JuLI Stage, Real-Time Cell History Recorder ©2017 NanoEnTek Inc.

  • Page 19: Adjustment Channel

    Before Start Adjustment Channel • To optimize the contrast, drag to adjust the Exposure, Brightness, LED Power and D-Phase in each channel - Exposure range: 5 ms ~ 500 ms - Brightness range: 0 ~ 40 - LED power range: 1 ~ 10 - D-Phase: 0 ~ 10 Drag Image •...

  • Page 20: Zoom-In & Zoom-Out

    Magnified navigation zoom in Magnified navigation zoom in Magnified navigation zoom in • Scroll out to zoom out from the view field in both real view and captured imags JuLI Stage, Real-Time Cell History Recorder ©2017 NanoEnTek Inc.

  • Page 21: Capture And Overlay

    Before Start Capture and Overlay • Capture and Overlay allows users to get both single channel and multiple channel images. • Edit the Brightness and Contrast of each channel • Press save button and choose image format and input name of image Time Stamp •...

  • Page 22: Operation

    Move to P01/P96 position Setup new P01/P06 location Complete of auto calibration Reset to default to setup new location Close auto calibration window to finish Regulate focus and movement of stage JuLI Stage, Real-Time Cell History Recorder ©2017 NanoEnTek Inc.
  • Page 23 Operation If P01 capture site is not locate to outside of image window you should reset P01 Auto Location and P96 Location. Calibration ☞ NOTE: There may variation in each C1 IFC vessel due to manufacture procedure and material of C1 IFC vessel 1.
  • Page 24 • The software automatically calibrate capture sites serially from P01, P96, P48, P24, P72 to P01 (Example: C1 IFC) • After the calibration is completed and click OK then move to Image screen JuLI Stage, Real-Time Cell History Recorder ©2017 NanoEnTek Inc.

  • Page 25: Manual Calibration

    Operation The software calibrate the spatial coordinates (X/Y-axis and Z-axis) of Manual specific capture sites after selection of specific sites Calibration 1. Start Calibration 2. Fix X/Y-axis 3. Fix Z-axis 4. Next Step 5. Completed • In the calibration screen, click Start Calibration, then stage will move to the first position (P01) Adjust X/Y-axis and Z-axis I Click Fix X/Y-axis then Z-axis...
  • Page 26 STEP 3: P48 STEP 4: P24 STEP 5: P72 STEP 6: P49 Complete of manual calibration • After finish of coordinates for all Steps display “OK I OK” , click Completed JuLI Stage, Real-Time Cell History Recorder ©2017 NanoEnTek Inc.

  • Page 27: Scan Ifc I

    Operation Scan tab allows to scan and capture images of all of the capture sites in Scan IFC I IFC with 4 channels (Bright-field, GFP, RFP. DAPI) 1. Enter Project Name 2. Select Channel 3. Select Data Pathway 4. Select Image Format 5.
  • Page 28 Operation Scan IFC I Adjust Camera Settings Drag to adjust the Exposure, Brightness, or LED Power settings Input fi le name Click project and input fi le name and click OK JuLI Stage, Real-Time Cell History Recorder ©2017 NanoEnTek Inc.
  • Page 29 Operation Stitching Scan IFC II Scan tab allows to make stitching images all of the capture sites in IFC with 4 channels (Bright-field, GFP, RFP. DAPI) 1. Turn on Stitching 2. Input number of image by Column X Row 3. Start Example of 2 x 1 stitched image...
  • Page 30 - # of images mean total number of images - Depth (µm) means each depth of image i.e) # of image – 5 Depth (µm) – 5 Total depth 10.0 µm ~ -10.0 µm JuLI Stage, Real-Time Cell History Recorder ©2017 NanoEnTek Inc.

  • Page 31: Analysis

    Analysis 1. Click Data file from data path How to 2. Select each cell to check Analysis 3. Export data Click data file Click data file from date path Select each Cell Click each cell to check or use Column x Row or Index to move...
  • Page 32 Selected images in each folder are exported to selected data pathways Check image II Software save all images including merged images by each channel to the All images folder. You can fi nd under TIMELAPSE folder JuLI Stage, Real-Time Cell History Recorder ©2017 NanoEnTek Inc.

  • Page 33: Settings

    Settings JuLI Stage checks the latest version of software or fi rmware and automatically display the information window Software and Firmware information is used to update through USB or network connection How to change objective lens is mentioned at page 14 Mouse control is used for changing basic function of mouse.

  • Page 34: Map Calibration

    3. Calibration distance 4. Adjust axis between adjacent images 5. Completed Horizontal axis mode If you click Horizontal axis mode, light green window is appeared right side of live image pane. JuLI Stage, Real-Time Cell History Recorder ©2017 NanoEnTek Inc.
  • Page 35 Settings Calibration • Calibration distance allow adjust images between image under light green window and adjacent image. ☞ NOTE: The right side adjacent image of light green window (which was outside of live image pane) is appeared to adjust image. Drag right side image to adjust horizontal axis Vertical axis mode •...
  • Page 36 ☞ NOTE: The bottom side adjacent image of light green window (which was outside of live image pane) is appeared to adjust image. Drag bottom side image to adjust vertical axis JuLI Stage, Real-Time Cell History Recorder ©2017 NanoEnTek Inc.

  • Page 37: Repackaging

    Repackaging How to install the locking module 1. Select the Setting tap. 2. Select the Locking Set button. When press this button, stage and fi lter changer will move to available position for locking module. 3. When fi nished stage moving, install the Locking module in the hole.

  • Page 38: Cleaning And Maintenance

    Never disassemble or service the JuLI™ Stage by yourself. Unauthorized repairs may damage the JuLI™ Stage or alter its functionality, which will void your warranty. Contact sales@nanoentek.com or your local the JuLI™ Stage distributor to arrange for service. IMPORTANT! The JuLI™ Stage is optimized into standard size of cell culture incubator.

  • Page 39: Troubleshooting

    Troubleshooting Intstallation JuLI Stage does not • Check on/off switch on back side of main device. power up • Check power source or contact your distributor. LCD screen is black • Move the mouse. • Check if power supply is connected and power switch is on.

  • Page 40: Warranty

    Warranty NanoEnTek provides 1-year warranty service for defects of material and workmanship. If any defects occur in the JuLI™ Stage, NanoEnTek provides free repair service for the defec- tive parts at its discretion. The following defects, however, are specifically excluded: 1.

  • Page 41: Product Components 5 Safety Precautions

    Safety precautions Review and follow the safety instructions below : • If water or other material enters the instrument, adaptor, or power inlet, disconnect the power cord and contact a service person. For operating environment, refer to Product Specifications. • Do not touch the main plug or power cord with wet hands. •...
  • Page 42 Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense. JuLI Stage, Real-Time Cell History Recorder ©2017 NanoEnTek Inc.

  • Page 43: Product Overview 6 Product Specifications

    Product specifications Items Specification Light source Blue, Green, UV LED (Intensity adjustable) Objective lens 4 X, 10 X & 20 X + Digital Zoom Fluorescence 3 fluorescence DAPI: Excitation 390/40, Emission 452/45 GFP: Excitation 466/40, Emission 525/50 RFP: Excitation 525/50, Emission 580LP High-sensitivity monochrome CCD (Sony sensor 2/3”) Camera 1,936 x 1,456 pixels (2.8 M), 53 FPS, 14 bit...

  • Page 44: Features 7 Ordering Information

    · Power cord x 4ea (USA, Europe, Australia, UK) SOS1000 Single-cell Observation System · USB drive 4GB · User manual (Optional) JOS1000 JuLI Stage Operation System · USB drive 4GB · User manual (Optional) JuLI Stage, Real-Time Cell History Recorder ©2017 NanoEnTek Inc.
  • Page 45 Ordering information Cat. No. Description Contents JO0004 JuLI™ Stage, Objective lens (4 X) Magnification: 4 X, NA: 0.16 (Optional) JO0010 JuLI™ Stage, Objective lens (10 X) Magnification: 10 X, NA: 0.3 (Optional) JO0020 JuLI™ Stage, Objective lens (20 X) Magnification: 20 X, NA: 0.45 (Optional) JVH001 JuLI™...
  • Page 46 851-14, Seohae-ro, Paltan-myeon, Hwaseong-si, Gyeonggi-do, 18531, Korea Tel : +82-2-6220-7940 Fax : +82-2-6220-7999 NanoEnTek Inc. (USA) 240 Bear Hill Road, Suite 101, Waltham, MA 02451, USA Tel : +1-781-472-2558 Fax : +1-781-790-5649 Email sales@nanoentek.com Website www.julistage.com www.nanoentek.com JuLI Stage, Real-Time Cell History Recorder ©2017 NanoEnTek Inc.
  • Page 47 Single-cell Observation System User Manual NESMU-JSTSC-001E(V.0.1) NanoEnTek Inc. 851-14, Seohae-ro, Paltan-myeon, Hwaseong-si, Gyeonggi-do, 18531, Korea Tel : +82-2-6220-7940 Fax: +82-2-6220-7999 NanoEnTek Inc. (USA) 240 Bear Hill Road, Suite 101, Waltham, MA 02451, USA Tel : +1-781-472-2558 Fax: +1-781-790-5649 Email sales@nanoentek.com Website www.julistage.com...

Table of Contents