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NanoEnTek JuLI FL User Manual

Fluorescence cell history recorder
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FL
FL
NESMU-JUF-001E (V.1.0)
Fluorescence Cell History Recorder
User
Manual
NanoEnTek Inc. (HQ)
12F, 5, Digital-ro 26-gil, Guro-gu, Seoul, 08389, Korea
Tel. +82-2-6220-7940
Fax. +82-2-6220-7721
NanoEnTek USA Inc.
5627 Stoneridge Drive Suite 304, Pleasanton, CA 94588, USA
Tel : +1-925-225-0108, +1-888-988-0108(Toll free)
Fax : +1-925-225-0109
Email
sales@nanoentek.com
Website
www.nanoentek.com

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Summary of Contents for NanoEnTek JuLI FL

  • Page 1 NESMU-JUF-001E (V.1.0) Fluorescence Cell History Recorder User Manual NanoEnTek Inc. (HQ) 12F, 5, Digital-ro 26-gil, Guro-gu, Seoul, 08389, Korea Tel. +82-2-6220-7940 Fax. +82-2-6220-7721 NanoEnTek USA Inc. 5627 Stoneridge Drive Suite 304, Pleasanton, CA 94588, USA Tel : +1-925-225-0108, +1-888-988-0108(Toll free)

  • Page 2: Table Of Contents

    42 - 46 Set up parameter Control LED power Setup date & time Set up dilution factor 43 - 44 Set up image format Update software & firmware Check the disk space JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.

  • Page 3: Product Components

    Product components JuLI™ FL is shipped with the following components. Please check all components listed below when an instrument was delivered. If any item is missing or damaged, contact your local distributor or e-mail sales@nanoentek.com. Scope unit Station unit Power cord...

  • Page 4: Product Overview

    • Intuitive graphic user interface & LCD touch screen • Compact size optimized for an cell culture incubator • Simple steps for the system setups and the operations • No calibration & maintenance required JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.

  • Page 5: Installation

    Installation Live-cell images from various cell culture dishes are directly Installing captured in a cell culture incubator. JuLI™ FL This compact design allows an instrument to be installed in an incubator, and prevents the contamination by maintaining a sterile environment. Also, the intuitive graphic user interface provides quick and convenient use in a cell culture incubator while capturing time-lapse imaging.
  • Page 6 5. When JuLI™ FL is ready to use, start JuLI™ FL by turning the ON/OFF switch on and pressing the Power button on the station unit. JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.

  • Page 7: Description

    Description <Scope unit> Front view Focus knob : is used to adjust the image quality to obtain better cell images. Stage : place sample here. : JULI-FLG04 : Green, Bright : JULI-FLR04 : Red, Bright <Station unit> LCD touch screen : Contains buttons for necessary functions and displays the images.
  • Page 8 : connect to external monitor. (resolution: 1366 X 768 pixels) : make sure to turn off an instrument before connecting. < Scope unit > Connection port < Station unit > Display port USB port Power button JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.
  • Page 9 Description <Scope unit> Rear view : is machinery cooling system. <Station unit> Power inlet ™ : connects JuLI FL to an electrical outlet using the supplied power cord and the appropriate plug (based on the electrical outlet configuration in your country). On/off switch : is main power switch.
  • Page 10 Brightness D-phase Confluence Save Merge Capture Scale bar Monitoring menu User interface Monitoring menu Graph Br/FL mode Scope unit channel Monitoring Monitoring data Monitoring Rec. button Stop button setting infomation movie JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.
  • Page 11 Description Data menu User interface Monitoring movie Data menu Data list Graph Rename button Multi select button Screen Delete Save to Monitoring data information Zoom bar shot button button Settings menu User interface Scope unit Sensitivity Settings menu channel & Background level power Dilution Image...
  • Page 12 Accurate and fast counting result: Provides counting result without focus variation and regardless of clumped cells in less than 10 seconds (Guaranteed the results and counting time using Auto focus function.). JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.

  • Page 13: Operation

    Operation 1. Press the Power button to start JuLI™ FL. Focusing The main screen will be displayed. Adjust the focus 2. Place the sample on the stage. 3. Check the channel by pressing the Channel tab if the 2 scope is connected. Select the Bright or the Fluorescence mode. 4.
  • Page 14 The images below show the reference images to adjust focus. Du-145 / Parameter A Exposure 4 Good setting Brightness 1 Exposure 6 Normal setting Brightness 3 Exposure 6 Bad setting Brightness 5 JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.

  • Page 15: Auto Focus

    Operation 1. Adjust the focus of JuLI™ FL (Refer to page 14 - 15) Focusing Auto focus 2. After adjusting focus, press the Auto focus button to get more accurate focus without individual variations · The Auto focus is the auto-focusing function which automatically senses the focus optimized for the analysis software of the JuLI™...

  • Page 16: Roi Auto Focus

    JuLI™ FL IMPORTANT It is strongly recommended to use ROI Auto focus function after adjusting a focus. If not, it might be the cause of, incorrect counting results or viability JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.

  • Page 17: Confluence

    Operation 1. Adjust the focus of JuLI™ FL (Refer to page 14-15). Focusing Confluence 2. Press the Confluence button (Bright mode only). (Bright mode only) 3. Value of confluence would be shown. x1 x1 · If the value of confluence should be more accurate, try again after adjusting parameters in the Setting menu (Refer to page 40).
  • Page 18 (Bright mode only) Du-145 / Parameter A Good setting Exposure 4 Brightness 1 Confluence 35.11% Normal setting Exposure 6 Brightness 3 Confluence 36.22% Bad setting Exposure 6 Brightness 5 Confluence 43.03% JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.

  • Page 19: Bright Image Capture

    Operation 1. Adjust the focus of JuLI™ FL (Refer to page 14-15). Focusing Bright image 2. Press the Br button and the Capture button to acquire an image. capture x1 x1 · Press the Preview button to recapture if necessary. 3.

  • Page 20: Fluorescence Image Capture

    Type the file name in the blank. 4. Check confirmation message ‘Capture complete’ . Press the OK button. 5. The saved data can be checked in Data menu (Refer to page 27). JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.

  • Page 21: Merged Image Capture

    Operation 1. Capture the bright image and the fluorescence image Focusing (Refer to page 20-21). Merged image capture 2. Press the Merge button to show the merged color image. x1 x1 3. If necessary, press the Save button to save the merged image.

  • Page 22: Monitoring

    2. Adjust the focus of JuLI™ FL and select the region that should be monitored (Refer to page 14-15). Press the Setting button. 3. Type the file name in the blank and the press the Save button. JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.
  • Page 23 Operation 4. Choose the monitoring mode among the Growth rate, the Monitoring Wound healing and the Movie only. · Movie only means just monitoring cells without calculating the cell confluence value. 5. After choosing channel 1or channel 2, set movie options (Choose both channel 1 and channel 2 in case of dual monitoring.).
  • Page 24 JuLI™ FL displays live cell images continuously during recording. · If necessary to adjust the focus during monitoring, press the Pause button, and change the focus, the exposure and the brightness in the Focusing menu. JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.
  • Page 25 Operation · Dual scope channel can be checked at once by pressing the Dual view button Monitoring when 2 scope unit is connected to the station unit. To close dual view mode, double click it. (or press the Close button.) ·...

  • Page 26: Data

    Merge) The control sample and experimental sample data can be found in the subfolders. < Data category> · If you double tap main folder of dual monitoring data, summary data view will show up. JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.
  • Page 27 Operation 3. Data can be opened or shown by double tapping folder name Data in Data list or pressing the Enter button. Open data 4. The captured image can be shown by tapping its file name. The images can be checked by pressing the Play button. 5.

  • Page 28: Screenshot

    5. After that, double tap to save the selected region. 6. Type the name in the blank, then press the Save button. 7. Check the confirmation message ‘Screenshot is saved successfully!’ . Press the OK button. JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.

  • Page 29: Save To Usb

    Operation 1. Connect the USB drive to the station unit. Data · The Save to USB button will be activated by connecting USB drive to the station unit. Save to USB 2. Select the folder or file by tapping it once. 3.

  • Page 30: Rename

    3. Type new name of folder or file in the blank. Then press the Save button. 4. Check the confirmation message ‘Rename complete’ and the press the OK button. JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.

  • Page 31: Delete

    Operation 1. Select folder or file that should be deleted from the instrument. Data Delete · To delete several folders or files at once, select all of them using the Multi select button. 2. Press the Delete button to delete selected folders or files.
  • Page 32 Operation 4. Check the confirmation message ‘Delete complete’ . Data Press the OK button. Delete JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.

  • Page 33: Counting

    Operation • Mix well 10 µL of sample and the 10 µL of 0.4% trypan blue stain Counting (Bright mode). Sample- • Place the Fluorescence sample on the scope unit preparation (Fluorscence mode). 1. Load 10 µL of sample mixture on the counting slide (side A) using a pipette.

  • Page 34: Auto Focus

    It is strongly recommended to use Auto focus function after adjusting a focus. If not, it might be the cause of, - taking more time for counting (more than 10 seconds) - incorrect counting results or viability JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.

  • Page 35: Bright Counting

    Operation 1. Adjust the focus of JuLI™ FL (Refer to page 14-15). Counting If necessary, press the Auto focus to get more accurate focus Bright counting without individual variations. (Stained by trypan blue) 2. Press the Br Counting button for the cell counting. ·...
  • Page 36 5. Cell counting result will be shown with cell size distribution graph. If you are not satisfied with the result, then try again after adjusting minimum and maximum cell size. JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.

  • Page 37: Fluorescence Counting

    Operation 1. Adjust the focus of JuLI™ FL (Refer to page 14-15). Counting If necessary, press the Auto focus to get more accurate focus Fluorescence without individual variation. counting 2. Press the FL Counting button for the cell counting. · The Br counting button and the FL counting button are activated in the Bright mode. ·...
  • Page 38 Fluorescence tap. Fluorescence counting result tap Graph Gating background Fluorescence cell (blue bar) Non- Fluorescence cell (red bar) Cell size gating bar Counting results JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.
  • Page 39 Operation 5. The counting results including the total cell, fluorescence Counting cell, non-fluorescence cell, average cell size show up on the Fluorescence Fluorescence Gating tap. counting Fluorescence gating tap Graph After Fluorescence gating (Non-Fluorescence cell) After Fluorescence gating (Fluorescence cell) Fluorescence intensity Fluorescence...

  • Page 40: Save To Usb

    < Bright counting result save > < Fluorescence counting result save > 4. If the confirmation message is shown, the saving process is completed. JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.

  • Page 41: Settings

    Operation Settings menu works for each scope unit if 2 unit is connected to Settings the station unit. Press the Channel tab to select each scope unit. Settings Set the recommended parameter values for corresponding to cell line. The default value is A (Bright mode only). Set up parameter Sensitivity : Refers to the contrast of the objects from the background.

  • Page 42: Control Led Power

    The Default value is 2 for the Set up dilution bright counting using the trypan blue staining method and 1 for factor the fluorescence counting. Fluorescence JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.

  • Page 43: Set Up Image Format

    Operation Set the dilution factor corresponding to the experiment condition Setting and the Apply button. Set up dilution factor The JuLI™ FL supports various image formats including JPEG, Setting BMP, TIFF, and PNG. Select one as mostly utilized format. The Set up image default format is JPEG.

  • Page 44: Update Software & Firmware

    Update button. If you have any questions about the software updates, contact sales@nanoentek.com. 2. Visit JuLI™ FL website at www.nanoentek.com to download updated software which will be supplied as a .zip file to your desktop. 3. Unzip the JuLI™ FL Update.zip file and save the software in a USB drive.

  • Page 45: Check The Disk Space

    Operation Check the disk space of JuLI™ FL. Setting If no disk space left, the saved data should be deleted in Data menu Check the disk (Refer to page 32). space...

  • Page 46: Pc Software

    Then open the file “JuLI™ FL PC Setup”. 2. The start-up dialogue of the software, as shown like left image, will appear. 3. Press the Next button to start installation. JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.
  • Page 47 Operation 4. If you want to change PC software installation folder, press the Installation Browse button and choose the location that you want. 5. After choosing the installation folder, press the Install button to proceed with the installation. 6. The PC activates the “Installation of the Software”...

  • Page 48: Function Guide

    Function guide Home Confluence Movie Counting Dual view Open Save as Export Dual project creation Data list Graph Preview Zoom bar Image Save with Insert window information scale bar scale bar JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.

  • Page 49: Home

    Operation PC software Home 1. Press the Open button. PC software Open data · Folders and files can be opened by dragging and dropping on data list window directly. 2. Browse the folder and select it.
  • Page 50 • In the case of dual monitoring data, control sample folder and experimental sample folder can be checked easily using the Channel tab. JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.
  • Page 51 Operation 5. The image can be magnified using the Zoom bar. PC software Open data 6. Select the viewing region by dragging the preview window. 7. Press the Insert scale bar button to activate the scale bar on the preview window, if necessary. •...

  • Page 52: Dual Project Creation

    3. Press the Make New Folder button and type the folder name of the dual project. 4. The dual project folder will be created after working process is done. JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.

  • Page 53: Export Data

    Operation 1. Press the Export button. Information of each image in folder PC software can be exported. Export data 2. Select the folder where exported file should be saved.Type the file name for saving exported file. ☞ The file will be saved as csv file, which is compatible with Excel and Notepad programs.

  • Page 54: Confluence

    5. Confluence graph would be redrawn. Time ( Hour : Min ) Time ( Hour : Min ) 6. Press the Save as button to save the images with the new confluence values. JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.

  • Page 55: Edit Movie File

    Operation 1. Press the Movie menu. PC software Edit movie file 2. Recorded movie can be shortened easily through the JuLI™ FL PC Software. Set the start point and end point by moving the bar. 3. Check the Apply box to edit the movie. 4.

  • Page 56: Recount Bright Data

    3. Select a File name of the bright image in the data list and press the Counting button to recalculate. 4. The counting results including the total cell, live cell, dead cell, and average cell size show up on the Bright tap. JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.
  • Page 57 Operation 5. Determine the value for the minimum cell size and the PC software maximum cell size touching the control bar ( ) to adjust Recount bright data minutely. · The set-value will be immediately reflected in the Counting results.

  • Page 58: Recount Fluorescence Data

    · The dilution factor is set as “1” in this sample data. 3. Press the Multi selection for FL counting button and select a pair of data including one bright image and one fluorescence image. 4. Press the Counting button to recalculate. JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.
  • Page 59 Operation 5. The counting results including the total cell, live cell, dead PC software cell, and average cell size show up on the Bright tap. Recount fluorescence data 6. The counting results including the total cell, fluorescence cell, non-fluorescence cell and average cell size show up on the Fluorescence tap.
  • Page 60 Recount Fluorescence tap. fluorescence data <Before fluorescence gating> <After fluorescence gating> · The set-value will be immediately reflected in the Counting results. JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.

  • Page 61: Dual View

    Operation 1. Dual monitoring data can be checked at once in the Dual PC software view menu. Press the Dual view menu. Dual view 2. Summary of the dual monitoring will be shown. 3. Select the modes (Br or FL) of each channel. ·...
  • Page 62 5. Type a file name of the merged movie and press the Save PC software button. Dual view 6. The merged movie file will be created after merging process is done. JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.

  • Page 63: Cleaning & Maintenance

    Never disassemble or service JuLI™ FL by yourself. Unauthorized repairs may damage JuLI™ FL or alter its functionality, which will void your warranty. Contact sales@nanoentek.com or your local JuLI™ FL distributor to arrange for service. Always wipe surfaces with ethanol-soaked paper towels.

  • Page 64: Troubleshooting

    Can’t set the interval · Check the minimum interval (Refer to page 24). Problem for saving movie file · Check available volume of the hard disk. JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.
  • Page 65 Fail to capture · Check if the scope unit and the station unit are connected properly. Movie does not play on · Install the codec provided JuLI FL USB drive or windows media player. NanoEnTek website. Can not transmit the screen ·...

  • Page 66: Warranty

    Warranty NanoEnTek provides 1-year warranty service for defects of material and workmanship. If any defects occur in JuLI™ FL, NanoEnTek provides free repair service for the defective parts at its discretion. The following defects, however, are specifically excluded: Defects caused by improper operation.

  • Page 67: Safety Precautions

    Safety precautions Review and follow the safety instructions below : If water or other material enters the instrument, adaptor, or power inlet, disconnect the · power cord and contact a service person. For operating environment, Check product Specifications (Refer to page 70). ·...
  • Page 68 4. For more detailed information about disposal of your old appliance, please contact your city office, waste disposal service or visit our web-site, www. nanoentek.com. JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.

  • Page 69: Product Specifications

    Product specifications Environmental conditions Electrical input AC 100 - 240Va.c., 1.9A, 100W Frequency 50 / 60 Hz Installation site Indoor use only Operating 5 - 40 °C temperature Maximum relative 20 - 95 % humidity Instrument Magnification 4 X and digital zoom ( ~ 450 X) Image resolution 1280 x 960 pixels (1.3 M) Exported formats...

  • Page 70: Ordering Information

    Scope unit of JuLI FL Accessories XY stage JULI-BRTB Disposable Cell counting slide 50 slides (100 counts) JULI-BRS50 JULI-BRS1000 Cell counting slide 1,000 slides (2,000 counts) JULI-BRS5000 Cell counting slide 5,000 slides (10,000 counts) JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.

  • Page 71: Technical Support

    Technical support Visit our Website at www.nanoentek.com for : Technical resources, including manuals, FAQs, etc. · Technical support contact information · Additional product information and special offers. · For more information or technical assistance, please call or email. NanoEnTek Inc. (HQ)
  • Page 72 Copyright ©2013 by NanoEnTek Inc. All rights reserved. Published in Korea. Documentation : NESMU-JUF-001E Revision History : V.0.0 Date : Oct 2013 V.0.5 Date : Mar 2014 V.1.0 Date : Nov 2015 JuLI FL, Fluorescence Cell History Recorder ©2013 NanoEnTek Inc.

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