Table of contents Product components Product overview Installation Description 7-12 Operation 13-51 Focusing 13-19 Adjust the focus 13-14 Auto focus ROI Auto focus Confluence 17-18 Capture Monitoring 20-23 Data 24-32 Open data 24-26 Screenshot Save to USB 28-29 Rename Delete 31-32 Counting 33-36...
Product overview An increasing number of researchers are using live-cell imaging to study cellular functions. The JuLI™ Br, a smart Bright-cell movie analyzer, was designed for a variety of biological experiments. JuLI™ Br uses state-of-the-art optics to get live-cell images from various cell culture dishes.
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Installation 1. Place the instrument on a flat, dry and level surface after Installing unpacking the instrument. Allow at least 10 cm (4 inches) of JuLI™ Br free space at the back of JuLI™ Br for proper ventilation and prevention of overheating of electronic components. 2.
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Description <Station unit> Side view Power button : turn an instrument on and off by pressing the Power button. USB Port : insert a USB drive to save and transport images to PC. Display port : connect to external monitor.(resolution: 1366 X 768 pixels) : make sure to turn off an instrument before connecting.
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Description The user interface of JuLI™ Br, Live cell movie analyzer provides User new tools to expand cellular research through proprietary interface software. Insightful data can be acquired, such as proliferation Focusing menu assays. Focusing menu Scope unit channel Zoom in Navigation Zoom out Sacle bar...
Description Counting menu is a JuLI™ Br Counting software which can be User used with various cell types giving the accurate and fast counting interface results in the bright mode. Counting menu 1. Bright counting : to check for the cell viability by trypan blue staining method.
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Operation While viewing cells, use the Focus knob or Focus interface Focusing to further adjust the focus. Adjust the focus < coarse focus interface > < fine focus interface > · Type of focus interface would be switched by tapping circle point. ·...
Operation 1. Adjust the focus of JuLI™ Br. (Refer to page 12-13) Focusing 2. Touch the region of interest cell by hand on the screen ROI Auto focus · The ROI Auto focus is the auto-focusing function which automatically ™ Br senses the focus optimized for the analysis software of the JuLI IMPORTANT! It is strongly recommended to use ROI Auto focus function after adjusting a focus.
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Operation 6. Check confirmation message ‘Capture complete’ . Focusing Saved data can be opened in Data menu. (on page 20) Confluence The images below show the guideline for focus to obtain accurate cell confluence. Du-145 / Parameter A Good setting Exposure 4 Brightness 1 Confluence 35.11%...
Operation 1. Each scope channel can be viewed by pressing Channel tab Monitoring if 2 scope unit is connected to station unit. 2. Adjust the focus of JuLI™ Br and select the region that should be monitored using Focusing menu. (on page 11-12) Press Setting button.
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Operation · Time interval should be set up more than 1 minute for the best quality Monitoring result in case of growth rate and wound healing. but in case of dual monitoring, more than 5 minutes is recommended. · The number of total images will be displayed automatically when data value of “Total Time”...
Operation 1 All of saved data could be checked in Data menu. Data · Monitoring data including movie file and time lapse images were saved Open data in each of folder set up by user. And other data except monitoring are saved in a default folder, ‘Captured Images’...
Operation 1. Insert USB drive to USB port. Data · If a USB drive is not inserted into USB port, you can not use this Save to USB function. 2. Select the folder or file by tapping it once. 3. Several folders or files can be selected at once using Multi select button.
Operation 1. If you want to change folder or file name, tap the file or folder. Data Rename · Rename function can be used for folders of monitored data and files in default folder ‘Captured Images’ . Not for each files in monitored data folder.
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Operation 1. Adjust the focus JuLI™ Br (Refer to page 12-13) Counting Auto focus 2. After adjusting focus, press the Auto focus button to get more accurate focus without individual variations. · The Auto focus is the auto-focusing function wich automatically senses the focus optimized for the analysis software of the JuLI™...
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Operation 1. Connect USB drive to the station unit. Counting · If a USB drive is not connected to the station unit, you can not use this function. Save to USB 2. Press the Save to USB button. 3. Type the folder name in the blank. Then press the Save button. ·...
If you have any questions about software updates, contact sales@nanoentek.com. 2. Visit JuLI™ Br website at www.nanoentek.com to download updated software which is supplied as a .zip file in your desktop. 3. Unzip the .zip file and save the software in a USB driver.
Operation PC software is designed to manage data from JuLI™ Br. PC software Introduction · User can review saved image from JuLI™ Br in the PC. · User can recalculate confluence of all images and edited movies. To install PC software, follow the directions as below : PC software Check to ensure that the PC is connected to Internet and then Installation...
Operation When PC software program starts, the interface as below image PC software will be shown. Function guide Preview window Open Save as Create Export Home Confluence Movie Dual view dual movie Zoom bar Image information Preview window Graph Data list...
Operation 1. Click the Open button. PC software Open data · Folders and files can be opened by dragging and dropping on List region directly. 2. Browse the folder or files and select it. 3. If data opened successfully, data list could be checked out as below image.
Operation 1. Click the confluence menu button. PC software Confluence 2. Select one image that needs recalculated confluence value from data list. 3. Adjust the image by changing parameters; recommended sensitivity level and background level corresponding to cell line. Parameter Cell line Recommendation A(Default)
Operation 1. Dual monitoring data can be checked at once using PC software Dual view menu button. Click Dual view menu button. Dual view 2. Summary of dual monitoring data will be shown. 3. Individual movie files from control sample and experimental sample can be made into one movie using Export dual movie button.
Operation 1. Individual movie files from 2 different single monitoring data PC software can be made into one movie using Create dual movie button. Create dual movie 2. Select 2 different single monitoring data folder for making movie. · Selected movie files should have been monitored under the same interval and total time.
Never disassemble or service JuLI™ Br by yourself. Unauthorized repairs may damage JuLI™ Br or alter its functionality, which will void your warranty. Contact sales@nanoentek.com or your local JuLI™ Br distributor to arrange for service. Always wipe surfaces with ethanol-soaked paper towels.
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JuLI Br PC software program. Setting 'JuLI Br Update' folder does · Download 'JuLI Br Update' folder on NanoEnTek not exist in the USB drive. website. (www.nanoentek.com) The update files do not exist · Check the files as below image. Then download in the USB drive.
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Fail to capture · Connect scope unit with station unit using connection cable again. Movie doesn’t play on · Install the codec provided by JuLI Br USB drive or windows media player NanoEnTek website. Can’t transmit the screen · Check the resolution of the outer monitor.
The following defects, however, are specifically excluded: Defects caused by improper operation. 2. Repair or modification done by anyone other than NanoEnTek or an authorized agent. 3. Damage caused by substituting with alternative parts. 4. Use of fittings or spare parts supplied by anyone other than NanoEnTek.
Safety precautions Review and follow the safety instructions below : Symbol Meaning Caution & Warning Protective earth (Ground) This instrument and consumables conforms to the Declaration of Conformity. This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules.
Technical support Visit the our Website at www.nanoentek.com for : Technical resources, including manuals, FAQs, etc. · Technical support contact information · Additional product information and special offers. · For more information or technical assistance, please call or email. NanoEnTek Inc. (HQ)
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