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NanoEnTek JuLi BR User Manual

Cell history recorder
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Summary of Contents for NanoEnTek JuLi BR

  • Page 2: Table Of Contents

    Table of contents Product components Product overview Installation Description 7-12 Operation 13-51 Focusing 13-19 Adjust the focus 13-14 Auto focus ROI Auto focus Confluence 17-18 Capture Monitoring 20-23 Data 24-32 Open data 24-26 Screenshot Save to USB 28-29 Rename Delete 31-32 Counting 33-36...

  • Page 3: Product Components

    1 EA 1 SET USB drive User manual Quick manual 4 GB 1 EA 1 EA 1 EA · Model JULI-BRSC <Scope unit> is only used in conjunction with the <Station unit>. JuLI Br, Cell History Recorder ©2012 NanoEnTek Inc.

  • Page 4: Product Overview

    Product overview An increasing number of researchers are using live-cell imaging to study cellular functions. The JuLI™ Br, a smart Bright-cell movie analyzer, was designed for a variety of biological experiments. JuLI™ Br uses state-of-the-art optics to get live-cell images from various cell culture dishes.

  • Page 5: Installation

    UV light may degrade components, including plastic. Damage from UV exposure is not covered under the manufacturer’s warranty. Always wipe surfaces with ethanol-soaked paper towels, not directly spray ethanol anywhere on JuLI™ Br. JuLI Br, Cell History Recorder ©2012 NanoEnTek Inc.
  • Page 6 Installation 1. Place the instrument on a flat, dry and level surface after Installing unpacking the instrument. Allow at least 10 cm (4 inches) of JuLI™ Br free space at the back of JuLI™ Br for proper ventilation and prevention of overheating of electronic components. 2.

  • Page 7: Description

    : is used to adjust the image quality to obtain better bright cell images. Stage : place sample here. White LED : provides bright image viewer. White LED Neck Stage Focus knob < Scope unit > < Station unit > touch screen JuLI Br, Cell History Recorder ©2012 NanoEnTek Inc.
  • Page 8 Description <Station unit> Side view Power button : turn an instrument on and off by pressing the Power button. USB Port : insert a USB drive to save and transport images to PC. Display port : connect to external monitor.(resolution: 1366 X 768 pixels) : make sure to turn off an instrument before connecting.
  • Page 9 Connection port : port to connect scope unit with station unit. <Scope unit> : machinery cooling system. < Scope unit > < Station unit > Connection port On/off switch Power inlet JuLI Br, Cell History Recorder ©2012 NanoEnTek Inc.
  • Page 10 Description The user interface of JuLI™ Br, Live cell movie analyzer provides User new tools to expand cellular research through proprietary interface software. Insightful data can be acquired, such as proliferation Focusing menu assays. Focusing menu Scope unit channel Zoom in Navigation Zoom out Sacle bar...
  • Page 11 Zoom bar select button button button button User interface Sensitivity Data & Time Settings Scope & Background level information menu unit channel Settings menu Disk space Software Firmware version version version power JuLI Br, Cell History Recorder ©2012 NanoEnTek Inc.

  • Page 12: Description

    Description Counting menu is a JuLI™ Br Counting software which can be User used with various cell types giving the accurate and fast counting interface results in the bright mode. Counting menu 1. Bright counting : to check for the cell viability by trypan blue staining method.

  • Page 13: Operation

    (» The scale of enlargement can be checked by pressing Scale bar tab.) 6. If focus of viewing region should be moved, tap a point on navigation window or drag preview window. JuLI Br, Cell History Recorder ©2012 NanoEnTek Inc.
  • Page 14 Operation While viewing cells, use the Focus knob or Focus interface Focusing to further adjust the focus. Adjust the focus < coarse focus interface > < fine focus interface > · Type of focus interface would be switched by tapping circle point. ·...

  • Page 15: Auto Focus

    JuLI IMPORTANT! It is strongly recommended to use Auto focus function after adjusting a focus. If not, it might be the cause of incorrect counting results or viability JuLI Br, Cell History Recorder ©2012 NanoEnTek Inc.

  • Page 16: Roi Auto Focus

    Operation 1. Adjust the focus of JuLI™ Br. (Refer to page 12-13) Focusing 2. Touch the region of interest cell by hand on the screen ROI Auto focus · The ROI Auto focus is the auto-focusing function which automatically ™ Br senses the focus optimized for the analysis software of the JuLI IMPORTANT! It is strongly recommended to use ROI Auto focus function after adjusting a focus.

  • Page 17: Confluence

    · If confluence should be recalculated, press Confluence button after pressing Preview button first. 4. Press the Capture button to acquire image with confluence. 5. Type the name of image that should be saved. Then, press Save button. JuLI Br, Cell History Recorder ©2012 NanoEnTek Inc.
  • Page 18 Operation 6. Check confirmation message ‘Capture complete’ . Focusing Saved data can be opened in Data menu. (on page 20) Confluence The images below show the guideline for focus to obtain accurate cell confluence. Du-145 / Parameter A Good setting Exposure 4 Brightness 1 Confluence 35.11%...

  • Page 19: Capture

    3. Type the name of image that should be saved. Then press Save button. 4. Check confirmation message ‘Capture complete’ . Press OK button. 5. Saved data can be opened in Data menu. (on page 20) JuLI Br, Cell History Recorder ©2012 NanoEnTek Inc.

  • Page 20: Monitoring

    Operation 1. Each scope channel can be viewed by pressing Channel tab Monitoring if 2 scope unit is connected to station unit. 2. Adjust the focus of JuLI™ Br and select the region that should be monitored using Focusing menu. (on page 11-12) Press Setting button.
  • Page 21 Warm up scope unit for 1 hour in incubator before making a movie. If not, it might be the cause of uncertain data or unclear movies. IMPORTANT! Cell culture flask should be wetted by the culture media before making movie. JuLI Br, Cell History Recorder ©2012 NanoEnTek Inc.
  • Page 22 Operation · Time interval should be set up more than 1 minute for the best quality Monitoring result in case of growth rate and wound healing. but in case of dual monitoring, more than 5 minutes is recommended. · The number of total images will be displayed automatically when data value of “Total Time”...
  • Page 23 Recorded data before getting in problem is saved in the disk of OK button instrument temporarily. So press if the movie recording should be continued. 8. Recorded data can be opened in Data menu. (page 20) JuLI Br, Cell History Recorder ©2012 NanoEnTek Inc.

  • Page 24: Data

    Operation 1 All of saved data could be checked in Data menu. Data · Monitoring data including movie file and time lapse images were saved Open data in each of folder set up by user. And other data except monitoring are saved in a default folder, ‘Captured Images’...
  • Page 25 List category or tapping folder name once and pressing Result category. 4. Recorded movie or image file can be shown by tapping its file name. Movie file can be checked by pressing Play button. JuLI Br, Cell History Recorder ©2012 NanoEnTek Inc.
  • Page 26 Operation 5. Magnify movie screen or image screen widely for Data convenience. Open data...

  • Page 27: Screenshot

    5. After then double tap the selected region. 6. Type the name for saving it, then press Save button. 7. Check confirmation message ‘Screenshot is saved successfully!’ . JuLI Br, Cell History Recorder ©2012 NanoEnTek Inc.

  • Page 28: Save To Usb

    Operation 1. Insert USB drive to USB port. Data · If a USB drive is not inserted into USB port, you can not use this Save to USB function. 2. Select the folder or file by tapping it once. 3. Several folders or files can be selected at once using Multi select button.
  • Page 29 5. Confirmation message ‘Save complete’ is shown, saving Data process is completed. Save to USB · Experimental sample data (channel 2) is included in control sample data (channel 1) folder in case of dual monitoring JuLI Br, Cell History Recorder ©2012 NanoEnTek Inc.

  • Page 30: Rename

    Operation 1. If you want to change folder or file name, tap the file or folder. Data Rename · Rename function can be used for folders of monitored data and files in default folder ‘Captured Images’ . Not for each files in monitored data folder.

  • Page 31: Delete

    · Only main folder can be deleted, not sub folders individually in case of dual monitoring data. 3. Press OK button when confirmation message shows up. JuLI Br, Cell History Recorder ©2012 NanoEnTek Inc.

  • Page 32: Data

    Operation 4. Check confirmation message ‘Delete complete’ . Data Delete...

  • Page 33: Counting

    The two chambers of the slide and labeled as "A" preparation and "B" for easy tracking of your samples. Each chambers is counted seperately. 2. Put the counting slide with the slide holder on the scope unit. JuLI Br, Cell History Recorder ©2012 NanoEnTek Inc.
  • Page 34 Operation 1. Adjust the focus JuLI™ Br (Refer to page 12-13) Counting Auto focus 2. After adjusting focus, press the Auto focus button to get more accurate focus without individual variations. · The Auto focus is the auto-focusing function wich automatically senses the focus optimized for the analysis software of the JuLI™...
  • Page 35 (Refer to page 42). 3. The counting results including the total cell, live cell, dead cell, and average cell size show up. Graph Gating background Dead cell (red bar) Live cell (blue bar) Counting results JuLI Br, Cell History Recorder ©2012 NanoEnTek Inc.
  • Page 36 Operation 1. Connect USB drive to the station unit. Counting · If a USB drive is not connected to the station unit, you can not use this function. Save to USB 2. Press the Save to USB button. 3. Type the folder name in the blank. Then press the Save button. ·...

  • Page 37: Settings

    HepG2 GH3 Hep3B Sensitivity level 9 A549 MCF7 Background level 2 SH-SY5Y SCN2.2 NIH-3T3 F9 HeLa DU-145 LNCaP Sensitivity level 10 Background level 2 U-2 OS Sensitivity level 10 ADSC Background level 8 JuLI Br, Cell History Recorder ©2012 NanoEnTek Inc.

  • Page 38: Update  Date & Time

    If you have any questions about software updates, contact sales@nanoentek.com. 2. Visit JuLI™ Br website at www.nanoentek.com to download updated software which is supplied as a .zip file in your desktop. 3. Unzip the .zip file and save the software in a USB driver.

  • Page 39: Check The Disk Space

    6. After updating, Press OK button to restart JuLI™ Br automatically. Check the disk space of JuLI™ Br. Setting If no disk space left, saved data should be deleted in Data menu. Check the disk (on page 20) space JuLI Br, Cell History Recorder ©2012 NanoEnTek Inc.

  • Page 40: Pc Software

    Operation PC software is designed to manage data from JuLI™ Br. PC software Introduction · User can review saved image from JuLI™ Br in the PC. · User can recalculate confluence of all images and edited movies. To install PC software, follow the directions as below : PC software Check to ensure that the PC is connected to Internet and then Installation...

  • Page 41: Installation

    5. After choosing installation folder, click “Install” to proceed with the installation. 6. The computer activates the “Installation of the software” If the installation is successful, the PC software program icon will appear on your desktop. JuLI Br, Cell History Recorder ©2012 NanoEnTek Inc.

  • Page 42: Function Guide

    Operation When PC software program starts, the interface as below image PC software will be shown. Function guide Preview window Open Save as Create Export Home Confluence Movie Dual view dual movie Zoom bar Image information Preview window Graph Data list...

  • Page 43: Home

    1. Press Insert scale bar button to insert scale bar on viewing PC software image, if it is necessary. Home Click Save with scale bar button to save image with scale bar information. JuLI Br, Cell History Recorder ©2012 NanoEnTek Inc.

  • Page 44: Open Data

    Operation 1. Click the Open button. PC software Open data · Folders and files can be opened by dragging and dropping on List region directly. 2. Browse the folder or files and select it. 3. If data opened successfully, data list could be checked out as below image.
  • Page 45 · In case of dual monitoring data, control sample folder and experimental sample folder can be checked easily using Channel tab. 5. The image can be magnified by tapping zoom bar. 6. Select the viewing region by dragging preview window. JuLI Br, Cell History Recorder ©2012 NanoEnTek Inc.

  • Page 46: Confluence

    Operation 1. Click the confluence menu button. PC software Confluence 2. Select one image that needs recalculated confluence value from data list. 3. Adjust the image by changing parameters; recommended sensitivity level and background level corresponding to cell line. Parameter Cell line Recommendation A(Default)

  • Page 47: Edit Movie File

    Time ( Hour : Min ) 2. Check Apply box to edit the movie. 3. Click Save as button if edited movie should be saved. 4. Select the folder to save edited movie and press OK button. JuLI Br, Cell History Recorder ©2012 NanoEnTek Inc.

  • Page 48: Dual View

    Operation 1. Dual monitoring data can be checked at once using PC software Dual view menu button. Click Dual view menu button. Dual view 2. Summary of dual monitoring data will be shown. 3. Individual movie files from control sample and experimental sample can be made into one movie using Export dual movie button.
  • Page 49 Operation 4. Type file name for merged movie and click Save button. PC software Dual view 5. Merged movie file will be created after merging process is done. JuLI Br, Cell History Recorder ©2012 NanoEnTek Inc.

  • Page 50: Create Dual Movie

    Operation 1. Individual movie files from 2 different single monitoring data PC software can be made into one movie using Create dual movie button. Create dual movie 2. Select 2 different single monitoring data folder for making movie. · Selected movie files should have been monitored under the same interval and total time.

  • Page 51: Export Data

    PC software be exported. Export data 2. Select the folder where exported file should be saved. Type the file name to save exported file. 3. Exported file can be found in folder. JuLI Br, Cell History Recorder ©2012 NanoEnTek Inc.

  • Page 52: Cleaning & Maintenance

    Never disassemble or service JuLI™ Br by yourself. Unauthorized repairs may damage JuLI™ Br or alter its functionality, which will void your warranty. Contact sales@nanoentek.com or your local JuLI™ Br distributor to arrange for service. Always wipe surfaces with ethanol-soaked paper towels.

  • Page 53: Troubleshooting

    · Cell culture flask should be wetted by the culture media before making movie. · Eliminate dust on a culture dish. · Check for and remove any condensation on the lid of the culture dish. JuLI Br, Cell History Recorder ©2012 NanoEnTek Inc.
  • Page 54 JuLI Br PC software program. Setting 'JuLI Br Update' folder does · Download 'JuLI Br Update' folder on NanoEnTek not exist in the USB drive. website. (www.nanoentek.com) The update files do not exist · Check the files as below image. Then download in the USB drive.
  • Page 55 Fail to capture · Connect scope unit with station unit using connection cable again. Movie doesn’t play on · Install the codec provided by JuLI Br USB drive or windows media player NanoEnTek website. Can’t transmit the screen · Check the resolution of the outer monitor.

  • Page 56: Warranty

    The following defects, however, are specifically excluded: Defects caused by improper operation. 2. Repair or modification done by anyone other than NanoEnTek or an authorized agent. 3. Damage caused by substituting with alternative parts. 4. Use of fittings or spare parts supplied by anyone other than NanoEnTek.

  • Page 57: Safety Precaution

    In the documentation for the user, a statement shall be included drawing attention to the fact that there may be potential difficulties in ensuring electromagnetic compatibility in other environments, due to conducted as well as radiated disturbances. JuLI Br, Cell History Recorder ©2012 NanoEnTek Inc.

  • Page 58: Safety Precaution

    Safety precautions Review and follow the safety instructions below : Symbol Meaning Caution & Warning Protective earth (Ground) This instrument and consumables conforms to the Declaration of Conformity. This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules.

  • Page 59: Product Specifications

    Dimension & weight Scope unit  :  300 x 190 x 188 mm, 4kg Station unit : 282 x 285 x 160 mm, 3.2kg Storage 320 GB Hard drive 4 GB USB drive JuLI Br, Cell History Recorder ©2012 NanoEnTek Inc.

  • Page 60: Technical Support

    Technical support Visit the our Website at www.nanoentek.com for : Technical resources, including manuals, FAQs, etc. · Technical support contact information · Additional product information and special offers. · For more information or technical assistance, please call or email. NanoEnTek Inc. (HQ)
  • Page 61 All rights reserved. Published in Korea. Documentation : NESMU-JUB-001E Revision History : V.0.0 Date : Oct 2012 V.1.0 Date : Jan 2013 V.1.5 Date : May 2013 V.1.6 Date : Jan 2014 V.2.0 Date : Nov 2015 JuLI Br, Cell History Recorder ©2012 NanoEnTek Inc.
  • Page 62 NESMU-JUB-001E (V.2.0) NanoEnTek Inc. (HQ) 12F, 5, Digital-ro 26-gil, Guro-gu, Seoul, 08389, Korea Tel. +82-2-6220-7940 Fax. +82-2-6220-7721 NanoEnTek USA Inc. 5627 Stoneridge Drive Suite 304, Pleasanton, CA 94588, USA Tel : +1-925-225-0108, +1-888-988-0108(Toll free) Fax : +1-925-225-0109 Email sales@nanoentek.com Website...

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