Appendix 1: Align The Instrument; Mechanical Alignment - Hitachi 3400-N Using Manual

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APPENDIX 1: Align the Instrument:

If you change the mechanical aperture, the accelerating voltage or the probe
current, specific alignments of the instrument must be performed.

Mechanical Alignment:

-At the desired
magnification, first turn the
X-Align knob
(Fig.A1-X)
side of the aperture control
until the beam reaches its
brightest.
-Hit "ABCC" to readjust
brightness.
-Repeat using Y-Align knob
can lose the beam entirely if you go too far. You must now perform the following
alignments:
Begin Beam Alignment:
-Begin with a magnification of 1000x –
5000x. Once you complete the alignment at that
zoom, repeat this whole process a second time at
twice your desired magnification.
-Apply the KV and probe current settings desired for
operation
-FIRST, perform normal focus and stigmation
operations without opening the align window.
The system must be relatively focused before
attempting to align it.
-Now open the alignment window.
-The functions of the "X and Y Dials"
your dial panel will change as you move down this
list of radio buttons to adjust various condensers and apertures.
at the
Figure A1. Mechanical Alignment
on the end of the column. BE CAREFUL! You
(Fig.A1-Y)
(Fig.A2)
(Fig.A3-X,Y)
-X
Figure A2. Beam Alignment
X
on
Figure A3. Alignment Controls
-Y
Y

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