Sakura Tissue-Tek Polar DM Operating Manual
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Sakura Tissue-Tek Polar DM Operating Manual

Frozen tissue section preparation instrument; cryostat microtome
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Operating Manual
Frozen Tissue Section Preparation Instrument
Cryostat Microtome

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Summary of Contents for Sakura Tissue-Tek Polar DM

  • Page 1 Operating Manual Frozen Tissue Section Preparation Instrument Cryostat Microtome...
  • Page 2 Introduction The frozen tissue section preparation ® instrument cryostat microtome Tissue-Tek ® Polar (herein after simply referred to as ® "the Polar instrument") is an instrument to section frozen tissues. Carefully read this Operating Manual ® and use the Polar instrument correctly so that its performance can be utilized fully.

  • Page 3: Table Of Contents

    Table of Contents Safety Precautions .................... f Chapter 1 Basic Knowledge of Instrument Basic Knowledge ..................1-1 Explanation of System ......................... 1-1 Installation Method ..................1-2 Environmental Factors ......................... 1-2 Name of Each Part (Exterior) ............... 1-3 Name of Each Part (Left/Right Panels, Display Area) ........ 1-5 Name of Each Part (Chamber) ..............
  • Page 4 Motorized Sectioning Tab Screen (DM only) ..........1-23 TRIM Mode Screen ..........................1-23 FINE Mode Screen..........................1-24 PAUSE Mode Screen ......................... 1-25 Tuning Tab Screen ..................1-26 Utilities Tab Screen ..................1-27 Other Items ........................... 1-28 History Tab Screen..................1-29 Various Settings ..................
  • Page 5 Software version ..........................1-52 Checking the Version........................1-52 Updating ............................1-52 Chapter 2 Operating Method Basic Operation Flow ................... 2-1 Logging On to the System ................2-2 Performing Pre-operation Checks ......................2-2 Turning On the Power .......................... 2-2 Logging On to the Instrument ....................... 2-3 Logging Off from the Instrument......................
  • Page 6 Preparing for Motorized Sectioning (DM only) ................... 2-26 Preparing for Sectioning ....................... 2-26 Selecting an Motorized Sectioning Mode ..................2-26 Moving the Specimen Head to the Sectioning Start Position ............2-27 Cutting the Specimen to Flat Surface (Rough Shaving) in the TRIM Mode ........2-27 Checking the Section Thickness....................
  • Page 7 Cleaning the Vacuum Suction Port ....................3-10 Cleaning the Anti-Roll Rake ....................... 3-11 Disassembling/Cleaning the Anti-Roll Rake ................. 3-11 Cleaning the Object Holder ........................ 3-11 Cleaning the Window Gasket ......................3-11 Replacing the Ozone Lamp ........................ 3-11 Cleaning the Air Intake Grille ......................3-12 Replacement of the Brine Solution (auxiliary cooling fluid) ..............

  • Page 8: Safety Precautions

    Safety Precautions Please appoint the "person responsible for handling the instrument." • The Polar® instrument requires special knowledge on the use and operation. Therefore, to use the instrument safely, please appoint the "person responsible for handling the instrument." • The "person responsible for handling the instrument" must directly be given handling instructions from the sales staff in charge at the time of instrument delivery.
  • Page 9 If foreign matters entered the instrument, stop the instrument as soon as possible and contact the nearest authorized Sakura distributor or representative. Also, the hole in the side is exclusively for only our service staff to operate. Please do not touch this hole.
  • Page 10 If the motor malfunctions or generates abnormal noise or the ozone lamp turns on erroneously, immediately turn off the power and contact the nearest authorized Sakura distributor or representative. Install the instrument in such a way that operation of the power switch will not be obstructed.
  • Page 11 WARNING • When disinfecting the instrument or chamber, do so in a well-ventilated place wearing protective gears. Also use the specified disinfectant/cleaning reagent. Using any other reagent may cause the instrument to corrode, fail, etc. For details, refer to Chapter 3, "Maintenance/Inspection."...
  • Page 12 • Inspect the system every 6 months. Contact the nearest authorized Sakura distributor or representative for the details of periodic inspection. • Be sure to lock the main body with the adjuster. When the handwheel is turned, the instrument may vibrate and cause unexpected accident. Lock the main body with the adjuster.
  • Page 13 OR if you notice other abnormalities, do not open the window but immediately turn the power OFF. There might be ozone still inside the chamber. Immediately contact the nearest authorized Sakura distributor or representative. [Refer to page (g) "Precaution When Performing Ozone Disinfection Cycle"] •...
  • Page 14 For this reason, when an infectious specimen has been handled, the source of the infection might not have been eliminated. Repeat ozone disinfection by manual operation. If an error recurs, contact the nearest authorized Sakura distributor or representative.

  • Page 15: Chapter 1 Basic Knowledge Of Instrument

    Chapter 1 Basic Knowledge of Instrument...

  • Page 16: Basic Knowledge

    Basic Knowledge Explanation of System The Tissue-Tek® Polar® is an instrument to freeze and section tissues and prepare sections. • Preparation of high-quality frozen specimens (D&DM type: Standard specification) Proper cooling of the specimen head (specimen holder) and area around the blade holder in the chamber allows for preparation of high-quality frozen tissue sections.

  • Page 17: Installation Method

    Installation Method This section provides information on determining a location for, and installing the Polar® instrument. Installation should be performed by qualified service staff working exclusively with the Polar® instrument. The Polar® instrument must be installed correctly to ensure proper operation and service. Read this Operating Manual carefully before operating the Polar®...

  • Page 18: Name Of Each Part (Exterior)

    Name of Each Part (Exterior) [1] Window and chamber The window is designed with an anti-fogging feature. To open the window, lift the handle and push it away from you. Refer to p. 1-6 for the details of the chamber. [2] Left panel This panel is used to operate the vacuum function, move the specimen head (specimen holder) forward and backward, and operate the trim function.
  • Page 19 [13] Draft generator (optional) This optional draft generator prevents contaminated air from diffusing outside the chamber. Refer to p. 2-34 for details. [14] Filtering power switch for draft generator This switch is used to supply power to the filtering mechanism of the optional draft generator. Refer to p. 2-34 for details.

  • Page 20: Name Of Each Part (Left/Right Panels, Display Area)

    Name of Each Part (Left/Right Panels, Display Area) [1] Vacuum key This switch is used to suction air form the vacuum inlet. Pressing this key once applies 100% suction force, while pressing it again adjusts the suction force to a desired level according to the setting. Refer to p.

  • Page 21: Name Of Each Part (Chamber)

    Name of Each Part (Chamber) [1] Cryobar and Cryobar removable plate Tissue samples can be tentatively stored here. Up to 15 (round) blocks of numbered Cryobar removable plates can be set. Refer to p. 1-12 for details. [2] Cryo+ This rapid freezing unit is used with the heat extractor to freeze specimens in the chamber. Refer to p. 2-5 for details.

  • Page 22: Handwheel

    Handwheel Overview of Handwheel Handle This handwheel is used to section tissues. Turn the handwheel clockwise from the front toward the back of the instrument. The specimen head will not back away, but still advance, even even when the handwheel is turned in the reverse direction. However, do not turn the handwheel in the reverse direction because doing so may result in an unexpected accident.

  • Page 23: Handwheel Lock Tab

    Handwheel Lock tab The handwheel has a safety lock mechanism. Manual sectioning or motorized sectioning is not possible when the handwheel is locked. The black lever at the rotating part of the handwheel is the handwheel lock tab. Unlocking the Handwheel Lock Unlock To unlock the handwheel, tilt the black lever toward you.

  • Page 24: Foot Switch (Dm Only)

    Foot Switch (DM only) Overview of Foot Switch By connecting the optional foot switch to the instrument, motorized Foot switch port sectioning can be started, stopped or paused not on the right panel, but using the foot switch. Note that the handwheel turns automatically according to the movement of the specimen head during motorized sectioning.

  • Page 25: How To Use/Lock The Foot Switch

    How to Operate the Foot Switch The foot switch is available in one of two types: TYPE 1 and TYPE 2. If you want to change the type, contact the nearest authorized Sakura distributor or representative. • TYPE 1 Step on the foot switch once to start motorized sectioning.

  • Page 26: How To Operate The Right Panel (Dm Only)

    How to Operate the Right Panel (DM only) How to Operate the Right Panel for Motorized Sectioning The right panel can be used to start, pause or stop motorized sectioning or adjust the motorized sectioning speed. Starting Motorized Sectioning Press the pause key ( ) and stop key () together. Motorized sectioning will be performed 30 times and then stop.

  • Page 27: Types Of Cryobars And Object Holders

    Types of Cryobars and Object Holders Types of Cryobar Removable Plates The Cryobar removable plate set on the Cryobar in the chamber is available in total four types. A number is marked at the hole in each removable plate. This is for easy identification because all you need is to use the Object Holder bearing the same number.

  • Page 28: Safety Functions

    2. Lock the handwheel to protect the tissue samples, and then turn off the power switch of the instrument and unplug the power cable from the power outlet. 3. Contact the nearest authorized Sakura distributor or representative. Resetting the Emergency Stop Turn Turn the emergency stop switch clockwise.

  • Page 29: Blade Guard

    Blade Guard The blade guard is used to prevent the tip of the disposable blade from being exposed. Be sure to use the blade guard when performing any task in the chamber that does not use the blade. 1. Tilt the Anti-Roll Plate and vacuum suction port toward you. 2.

  • Page 30: Recovery Processing

    Safety Functions Recovery Processing If power outage occurred or otherwise the power was cut off during processing and the processing stopped, recovery processing is performed once the system recovers. The following operations are performed: • Defrost • Manual ozone cycle •...

  • Page 31: Specifications

    Specifications General name Frozen Tissue Section Preparation Instrument Cryostat Microtome Brand name Tissue-Tek® Polar® Model POLAR-DM POLAR-D Instrument dimensions 785 mm (W) x 781 mm (D) x 1185 mm (H) (650 mm (W) without handwheel handle) Chamber dimensions 500 mm (W) x 360 mm (D) x 280 mm (H) (360 mm (D) excludes the evaporator) Instrument mass Approx.

  • Page 32: Standard Accessories/Options

    Standard Accessories/Options [15] 1-17...
  • Page 33 Standard Accessories Item name Product code Quantity Anti-Roll Rake F60-691-00 1 set Anti-Roll Plate F60-953-00 1 set Blade holder (D/DM type) F60-647-00 1 set Heat extractor (standard) F60-622-00 1 unit Parking plate (standard) O86-649-03 1 unit Tray (D/DM type) O86-695-00 1 unit Vacuum filter set (D/DM type only) F60-689-00...

  • Page 34: Explanation Of Screens

    Explanation of Screens Explanation of Screen Layout Screens consist of tabs, each offering a set of related functions. You can switch between various function screens by pressing the tabs on top of the current screen. Screens display information needed to perform operations. Depending on the situation, each tab screen shows error or emergency stop information, while the status display area at the bottom of the screen shows the icons for handwheel lock tab, foot switch, etc.

  • Page 35: Explanation Of Right-Of-Screen Button Area

    Explanation of Right-of-Screen Button Area The "Log On"/"Log Off" button, "Chamber Illumination" button, "Manual Ozone cycle" button and "Cryo+" button common to all tab screens are shown on the right side of the screen. • Log on/log off button Press the "Log On" button, and the screen will accept operation inputs.

  • Page 36: Section Tab Screen

    Section Tab Screen The [SECTION] Tab Screen is used for manual sectioning, where selecting the SECTION tab displays the current setting information and setting buttons needed to perform manual sectioning. The SECTION Tab covers three primary temperature setting modes. The "FREE Mode" screen lets you change the chamber temperature/specimen head temperature freely, while the "Temperature Setting 1 Mode/Temperature Setting 2 Mode"...

  • Page 37: Temperature Setting 1 Mode/Temperature Setting 2 Mode Screen

    Temperature Setting 1 Mode/Temperature Setting 2 Mode Screen The temperature setting 1 mode and temperature setting 2 mode start with the preset chamber temperature/specimen head temperature. Refer to p. 1-32 for information on how to change the settings. Only the section thickness can be changed in this mode. Current chamber Current specimen head temperature temperature...

  • Page 38: Motorized Sectioning Tab Screen (Dm Only)

    Motorized Sectioning Tab Screen (DM only) The [MOTORIZED] Tab Screen is used for motorized sectioning using the motor, where selecting the MOTORIZED tab displays the current setting information and setting buttons needed to perform motorized sectioning. The MOTORIZED tab covers three motorized sectioning modes. The "TRIM Mode"...

  • Page 39: Fine Mode Screen

    FINE Mode Screen In the FINE mode, final shaving is performed to the set section thickness. Motorized sectioning can be performed 30 times consecutively. To continue sectioning beyond that, press the start button again. The section thickness can be changed in this mode. The chamber temperature and specimen head temperature in the temperature setting mode selected on the SECTION Tab become effective.

  • Page 40: Pause Mode Screen

    Motorized Sectioning Tab Screen (DM only) PAUSE Mode Screen In the PAUSE mode, final shaving is performed to the set section thickness, just like in the FINE mode, but you can pause the sectioning every time the preset sectioning end position is reached, before the section is cut off.

  • Page 41: Tuning Tab Screen

    Tuning Tab Screen The [TUNING] Tab Screen lets you set the vacuum suction force, home position offset, and temperatures in temperature setting mode 1 or 2. Suction force setting button Home position offset Set chamber Set specimen head setting button temperature temperature Suction 100% setting button...

  • Page 42: Utilities Tab Screen

    Utilities Tab Screen The [UTILITIES] Tab Screen lets you start a manual defrost cycle, start/set manual shutdown, set schedule, set date/time, check the software, set the TRIM thickness, turn the draft generator on/off (only when the draft generator option has been set), and manipulate other items such as language selection, maintenance information viewing, cooling method setting, sound setting and account setting.

  • Page 43: Other Items

    Other Items Other items permit language selection, maintenance information viewing, cooling method setting, sound setting and account setting. Other items cannot be selected without permission of the manager or above. To set any of these items, log on as the manager or above. ●...

  • Page 44: History Tab Screen

    History Tab Screen The [HISTORY] Tab Screen lets you display and output the error log list and event log list. Event button * Toggle between the error log and event log. Error log list Page switching button (up/down) Output button Error button Toggle between the error long and event log Event log list...

  • Page 45: Various Settings

    Various Settings Setting the Section Thickness You can set the section thickness to section the specimen to. The section thickness can be set on the [SECTION] Tab Screen or [MOTORIZED] Tab Screen. Adjust the thickness according to the type and condition of the section. How to Change in the Manual Sectioning Mode 1.

  • Page 46: Setting The Trim Thickness

    Various Settings Setting the TRIM Thickness You can set the section thickness for specimen trimming (rough shaving) on the [UTILITIES] Tab Screen. The value set here applies to both manual sectioning and motorized sectioning. 1. Press the "TRIM Thickness Setting" button on the [UTILITIES] Tab Screen.

  • Page 47: Registering/Editing In The Temperature Setting Free Mode

    Registering/Editing in the Temperature Setting FREE Mode When the temperature setting FREE mode is selected on the [SECTION] Tab Screen, you can change the chamber temperature and specimen head temperature freely. Chamber temperature Specimen head temperature How to Set the Chamber Temperature setting button setting button 1.

  • Page 48: Registering/Editing In The Temperature Setting 1 / Temperature Setting 2 Mode

    Various Settings Registering/Editing in the Temperature Setting 1 / Temperature Setting 2 Mode The [TUNING] Tab Screen lets you register/edit the chamber temperature and specimen head temperature settings in the Temperature Setting 1 or Temperature Setting 2 temperature adjustment mode used on the [SECTION] Tab Screen.

  • Page 49: Setting The Home Position Offset

    Setting the Home Position Offset The Polar® instrument has the home position key on the left panel to let you move the specimen head to the preset sectioning start position with a touch of the key. Change the home position offset as desired or according to the height of the frozen block.

  • Page 50: Setting The Sectioning End Position

    Various Settings Setting the Sectioning End Position If motorized sectioning is performed by selecting the PAUSE mode on the [MOTORIZED] Tab Screen, sectioning will pause every time the preset sectioning end position is reached. Sectioning can be stopped at the adjusted position before the section is cut off. Note that this position can be changed regardless of whether the motorized sectioning mode is TRIM, FINE or PAUSE.

  • Page 51: Setting The Vacuum Suction Force

    Setting the Vacuum Suction Force Press the vacuum key on the left panel to suction debris. When a given suction force is currently set, pressing this button once starts suctioning at 100% and pressing it again switches the vacuuming to the desired suction force. Press the key one more time, and suctioning will end.

  • Page 52: Setting The Date/Time

    Various Settings Setting the Date/Time You can set the current date/time by pressing the "Time Setup" button on the [UTILITIES] Tab Screen. Time display format switching Month switching buttons button (24-hour/12-hour) Date selection area Exit button Set button Save button The currently set date/time is displayed.

  • Page 53: Setting The Date

    Setting the Date 1. Press the "Time Setup" button on the [UTILITIES] Tab Screen. 2. Touch the date to set in the date selection area. Use the "◄" button to switch to the previous month, or "►" button to switch to the next month.

  • Page 54: Setting A Schedule

    Various Settings Setting a Schedule You can switch between always-on operation and scheduled operation or set operation details. In always-on operation, the system performs the defrost cycle and ozone cycle (if set) at specified times without stopping the cooling. In scheduled operation, the instrument stops/runs and performs the defrost cycle and ozone cycle (if set) at set times.

  • Page 55: Setting The Cleaning Operation (Defrost Cycle/Ozone Cycle) Start Time

    Schedule button Setting the Cleaning Operation (Defrost Cycle/Ozone cycle) Start Time 1. Press the "Schedule" button on the [UTILITIES] Tab Screen. 2. Select the "Always-on operation" selector button. 3. Press the cleaning operation start time "Set" button. 4. The defrost cycle start time change screen appears. 5.

  • Page 56: Explanation Of Scheduled Operation Setting Screen

    Various Settings Explanation of Scheduled Operation Setting Screen Ready/shutdown setting Scheduled operation Always-on operation External save button button selector button selector button Operating day setting button Automatic ozone cycle Exit button Days off setting button box check button • Always-on operation/scheduled operation selector button The setting screen toggles between always-on operation and scheduled operation every time this button is pressed.

  • Page 57: Setting The Operation Ready/Shutdown Times

    Setting the Operation Ready/Shutdown Times Scheduled operation Ready/shutdown setting selector button button You can set the operation ready/shutdown times and days of the week when the system is shut down. 1. Press the "Schedule" button on the [UTILITIES] Tab Screen. 2.

  • Page 58: Setting Days Off

    Various Settings Scheduled operation selector button Setting Days Off A day off can be set for a single day. Refer to p. 1-42 if you want to set a specific day of the week as a day off every week. 1.

  • Page 59: Setting An Operating Day

    Setting an Operating Day Scheduled operation selector button Operating days can be set daily. Refer to p. 1-42 if you want to set a specific day of the week an operating day every week. 1. Press the "Schedule" button on the [UTILITIES] Tab Screen. 2.

  • Page 60: Using External Save

    Various Settings External save button Using External Save Pressing the "Schedule" button on the [UTILITIES] Tab Screen and then pressing the "External Save" button displays the external input/output screen (schedule). On this screen, you can "Backup," "Restore," "Export" and "Import" schedule information. If a USB memory device is not connected, the "Export"...

  • Page 61: Selecting The Language

    Selecting the Language 1. Press the "Miscellaneous" button on the [UTILITIES] Tab Screen. 2. On the other settings screen, press the "LANGUAGE" button. 3. Select a desired language from the language list, and the screen display will change accordingly. 4. Press the close button to close the screen. LANGUAGE setting button Close button Swap the display languages using the "◄"...

  • Page 62: Setting Sounds

    Various Settings Setting Sounds You can set the error sound, alarm sound, end sound and click sound. Changing the Sound Volume Sound setting button Close button 1. Press the "Miscellaneous" button on the [UTILITIES] Tab Error sound button Screen. Test button 2.

  • Page 63: Setting Accounts

    Setting Accounts You can set manager and user passwords and input/output password information from/to a USB memory device, etc. Up to four manager accounts and up to 20 user accounts can be set. Close button Account setting button Switching between the Manager List and User List Manager icon 1.

  • Page 64: Various Permission Table

    Various Settings Various Permission Table Available operation items are limited according to the permission granted to the logged-on user. This is explained by the table below. Operation item Manager User Everyone Reference page ○ ○ ○ Button on the Log on/log off 1-20, 2-2 ○...

  • Page 65: Using External Save

    External save button Using External Save Press the "Miscellaneous" button on the "UTILITIES Tab Screen" and then press the "Account" button on the other settings screen. Pressing the "External Save" button on the account setting screen displays the external input/output screen (password).

  • Page 66: Viewing And Outputting The History

    Various Settings Viewing and Outputting the History You can check and output the logs of errors and events experienced by the instrument. Log list switching button Checking and Outputting the Error Log Toggle between the error log and event log. 1.

  • Page 67: Software Version

    Checking the Version 1. Press the "Software" button on the [UTILITIES] Tab Screen. 2. Press the "Exit" button to return to the [UTILITIES] Tab Screen. Software button Updating Contact the nearest authorized Sakura distributor or representative. Exit button Update button 1-52...
  • Page 68 1-53...

  • Page 69: Chapter 2 Operating Method

    Chapter 2 Operating Method...

  • Page 70: Basic Operation Flow

    Basic Operation Flow The basic flow of preparing frozen sections using this system is shown below. For the details of related operation items, etc., refer to the applicable pages. ● Logging On to the System Details: p. 2-2 • Perform pre-operation checks. •...

  • Page 71: Performing Pre-Operation Checks

    Logging On to the System Performing Pre-operation Checks Perform the following checks before operating the Polar® instrument. • Checking the Waste Tank Drain the liquid waste, if collected, and clean the tank. Turning On the Power Turn on the power, if not yet turned on. The power switch is found on the right side face of the Polar®...

  • Page 72: Logging On To The Instrument

    (0 to 9) "Everyone," you can close the screen with the "Close" button. For the "AUTO LOGIN" setting, contact the nearest authorized Sakura distributor or representative. Log off button Logging Off from the Instrument To log off from the instrument, press the Log off button in the top right-hand corner of the screen.

  • Page 73: Preparing For Sectioning

    Preparing for Sectioning Checking the Temperature Settings Check if the chamber temperature and specimen head temperature are set correctly for sectioning. Change the temperature(s), if necessary. The temperatures set on the [SECTION] Tab Screen are also valid on the [MOTORIZED] Tab Screen. Current chamber Current specimen Checking the "FREE"...

  • Page 74: Preparing For Frozen Block Preparation

    Preparing for Sectioning Preparing for Frozen Block Preparation 1. Put the tissue sample (specimen) in the Cryomold [1]. 2. Put OCT compound by making sure it does not overflow [2]. Be careful not to let air bubbles enter. 3. Put the Object Holder on top [3]. Make sure the side bearing the number comes to the tab side of the Cryomold.

  • Page 75: Setting The Frozen Block On The Specimen Head

    Knob on the Object Holder Setting the Frozen Block on the Specimen Head Set the block you have just frozen on the specimen head. Tapered part When setting the block, put the blade guard on and be sure to lock the handwheel.

  • Page 76: Adjusting The Specimen Head

    Preparing for Sectioning Adjusting the Specimen Head Adjust the orientation (angle) of the specimen head so that the frozen block set on the specimen head will be sectioned correctly. This adjustment is also effective when you have difficulty cutting the specimen to flat surface (rough shaving).

  • Page 77: Changing The Disposable Blade

    Changing the Disposable Blade Change the disposable blade as necessary. For the disposable blade, you can use the High Profile type or Low Profile type by changing the adapter below the blade holder. Using the High Profile Type 1. If the Anti-Roll Plate is set, tilt the Anti-Roll Plate and vacuum suction port toward you.

  • Page 78: Adjusting The Lateral Movement Of The Blade Holder

    Preparing for Sectioning Adjusting the Lateral Movement of the Blade Holder You can slide the blade holder to the left and right. This way, the set disposable blade can be used fully. 1. Tilt toward you the blade holder left/right lever indicated in yellow and found on the left of the blade holder, to unlock the blade holder.

  • Page 79: Using The Low Profile Type

    Using the Low Profile Type 1. If the Anti-Roll Plate is set, tilt the Anti-Roll Plate and vacuum suction port toward you. 2. Tilt toward you the disposable blade mounting lever indicated in red, to unlock the disposable blade [1]. 3.

  • Page 80: Sectioning

    Sectioning Preparing for Manual Sectioning (Final Shaving) To perform manual sectioning, adjust the specimen head position and cut the specimen to flat surface first. Then, check the section thickness and perform sectioning (final shaving). Press the home position key to move the specimen head to the sectioning start position.

  • Page 81: Manually Sectioning The Specimen (Final Shaving)

    Manually Sectioning the Specimen (Final Shaving) When the specimen has been cut to flat surface, perform sectioning (final shaving). Release the TRIM key and turn the handwheel, and the specimen will be cut to the preset section thickness. Besides manual sectioning, you can use the Anti-Roll Rake or Anti-Roll Plate. For the details of manual sectioning, refer to the next item, "Sectioning Manually."...

  • Page 82: Sectioning With The Anti-Roll Rake

    Sectioning Sectioning with the Anti-Roll Rake Use of the Anti-Roll Rake eliminates the need to flatten the section with a brush, etc., so even a beginner can make sections relatively easily. 1. Cause the tip of the Anti-Roll Rake to lightly contact the bottom side (approx.

  • Page 83: Sectioning With The Anti-Roll Plate

    Sectioning with the Anti-Roll Plate Use of the Anti-Roll Plate eliminates the need to flatten the section with a brush, etc., so even a beginner can make sections relatively easily. 1. When the rough shaving is complete, tilt the Anti-Roll Plate toward the stage [1].

  • Page 84: After Processing

    After Processing Clean the Interior of the Chamber Using the Supplied Nozzle. If debris is collected in the chamber, operations may be affected and tissue samples may also be contaminated/infected. Clean the chamber using vacuum, etc., immediately after use. 1. Remove the vacuum head connection hose from the vacuum hose attachment port [1].

  • Page 85: Performing Cleaning Operation

    Performing Cleaning Operation Cleaning operation is the function to defrost automatically at the set time once a day. If the automatic ozone cycle is set, the ozone process is also performed. The defrost cycle is approx. 60 minutes, while the ozone cycle time is approx. 75 minutes. This operation is performed when always-on operation has been selected as part of the schedule on the [UTILITIES] Tab Screen.

  • Page 86: Cancelling Cleaning Operation

    After Processing Cancelling Cleaning Operation 1. To cancel cleaning operation, you must enter the password. 2. Pressing the "Stop" button displays the cleaning operation cancellation password confirmation screen. Once the correct password is entered, the cleaning operation cancellation confirmation screen appears. If cleaning operation ends while this screen is displayed, the screen closes automatically.

  • Page 87: Defrosting Manually

    Defrost button Defrosting Manually You can manually defrost by pressing the "Defrost" button on the [UTILITIES] Tab Screen. Select "Yes," and the defrost cycle will start and the defrost operation progress screen will appear. The screen shows the progress of operation. Once the processing is complete, the screen closes.

  • Page 88: Performing Ozone Cycle Manually

    After Processing Performing Ozone Cycle Manually You can perform the ozone disinfection cycle manually by pressing the "Manual Ozone Cycle" among the buttons on the right side of the screen. Select "Yes," and the ozone cycle operation progress screen will appear. If the window is open when "Yes"...

  • Page 89: Shutting Down The Instrument

    Shutting Down the Instrument Shutdown is the function to defrost at the set time and switch to the sleep screen. If automatic ozone cycle is set, the ozone process is performed in addition to defrost cycle during cleaning operation. The defrost cycle is approx. 60 minutes, while the ozone cycle time is approx. 75 minutes. Two shutdown methods are available, including "Manual Shutdown"...

  • Page 90: Manual Shutdown Under "Scheduled Operation

    After Processing 6. Pressing the "Set" button displays the setting time change screen. To change the hours, press the value of hours. Press the ""/" buttons among the "Value Change" buttons to change the value of hours. In the case of 24-hour display, hours is displayed in a range of 0 to 23 and minutes, as a multiple of 15 minutes.

  • Page 91: Cancelling Manual Shutdown

    Cancelling Manual Shutdown 1. To cancel manual shutdown, press the "Stop" button, and the display will change to the manual shutdown cancellation confirmation screen. 2. Select "Abort," and defrost cycle operation will be cancelled if the cleaning operation consists of the defrost cycle only, after which the screen will close.

  • Page 92: Shutting Down The Instrument Automatically

    After Processing Shutting Down the Instrument Automatically Automatic shutdown is effective when scheduled operation is set for the schedule on the [UTILITIES] Tab Screen. Refer to p. 1-39 for information on how to set this operation. In the case of always-on operation, defrost cycle is performed at the specified time without stopping freezing.

  • Page 93: Cancelling Automatic Shutdown

    Cancelling Automatic Shutdown 1. To cancel automatic shutdown, you must enter the password. 2. Pressing the "Stop" button displays the automatic shutdown cancellation password confirmation screen. Once the correct password is entered, the automatic shutdown cancellation confirmation screen appears. If automatic shutdown ends while this screen is displayed, the screen closes automatically.

  • Page 94: Other Operations

    As there might still be ozone in the chamber, the window stays locked and the error screen is displayed. The instrument cannot be used. Do not attempt to open the window, and immediately contact the nearest authorized Sakura distributor or representative. Ozone might still remain inside. 2-25...

  • Page 95: Preparing For Motorized Sectioning (Dm Only)

    Preparing for Motorized Sectioning (DM only) To perform motorized sectioning, select the motorized sectioning mode to be used on the [MOTORIZED] Tab Screen first. Next, adjust the position of the specimen head and cut the specimen to flat surface. Then, check the section thickness and perform sectioning (final shaving).

  • Page 96: Moving The Specimen Head To The Sectioning Start Position

    Other Operations Moving the Specimen Head to the Sectioning Start Position Move the specimen head to the position at which to start sectioning. The easiest way to move the specimen head is to press the home Home position key position key on the left panel to move the specimen head to the preset sectioning start position.

  • Page 97: Sectioning Automatically (Dm Only)

    Sectioning Automatically (DM only) When the specimen has been cut to flat surface, perform sectioning. Set the motorized sectioning mode to FINE or PAUSE. Unlock the handwheel and press the pause key ( ) and stop key () on the right panel together to perform sectioning.

  • Page 98: Adjusting The Anti-Roll Plate

    Other Operations Adjusting the Anti-Roll Plate You can adjust the angle (lateral inclination) and position of the glass of the Anti-Roll Plate. Adjusting the Forward/Backward Position of the Anti-Roll Plate Turning the forward/backward adjustment screw moves the Anti-Roll Plate forward/backward. Turn the screw counterclockwise to move the Anti-Roll Plate forward, or turn it clockwise to move the Anti-Roll Plate backward.

  • Page 99: Adjusting The Blade Holder

    Adjusting the Blade Holder You can perform a number of operations relating to the blade holder set in the chamber, such as changing the disposable blade, adjusting the blade angle (relief angle) and position, and setting the Anti-Roll Plate and vacuum suction port. Lever (red) (Disposable blade Blade guard...

  • Page 100: Adjusting The Blade Angle (Relief Angle)

    Other Operations Adjusting the Blade Angle (Relief Angle) You can adjust the blade angle by using an Allen wrench to Blade angle loosen counterclockwise (tilting the Allen wrench away from you) adjustment the blade angle adjustment bolt found on the left side face of the bolt (hex) blade holder.

  • Page 101: Removing The Blade Holder From The Chamber

    Removing the Blade Holder from the Chamber To adjust the blade angle (relief angle) of the blade holder or clean the blade holder, remove the blade holder from the chamber. Tilt toward you the blade holder mounting lever indicated in blue, to unlock the blade holder.

  • Page 102: Setting The Blade Holder In The Chamber (Cryo3 Adapter Is Used)

    Other Operations Setting the Blade Holder in the Chamber (Cryo3 Adapter Is Used) When the Cryo3 adapter is used, insert the blade holder mounting lever Locking hole indicated in blue into the dedicated Cryo3 locking hole to lock the blade (plugged with a holder.

  • Page 103: Turning On The Chamber Led Lights

    Turning on the Chamber LED Lights Press the "Chamber Illumination" button among the buttons on the right side of the screen to turn on the LED lights in the chamber. Press it again to turn off the lights. If not used for a specified period of time, the instrument enters the standby mode and the chamber LED lights turn off.

  • Page 104: Draft Generator

    Other Operations Draft Generator The draft generator provides the function to suction air in the top part of the chamber from both sides when the window is opened, in order to keep contaminated air from diffusing out of the chamber. Suctioned air is passed through the HEPA filter in the rear of the instrument and then exhausted to the outside.

  • Page 105: Setting The Parking Plate

    Cryobar Setting the Parking Plate The parking plate is used to cool the heat extractor and available in two sizes of standard and large (optional). For greater cooling efficiency, set the parking plate on the Cryo+ and press the "Cryo+" button to start cooling. Large (optional) Standard Cryobar partition plate...

  • Page 106: Plastic Object Holder

    Other Operations Plastic Object Holder The plastic Object Holder is made of material that does not conduct heat effectively. If the interior of the chamber cannot be cooled, using this Object Holder keeps the tissue sample frozen for a longer time because it is less affected by the temperature of the specimen head compared to the standard Object Holder.

  • Page 107: Adapter For Cryo3 Object Holder

    The block will freeze in approx. 45 seconds. 4. When finished, take out the frozen block. For inquiries on the PINO (frozen block preparation instrument), Cryo Holder and/or Hyfluid (cooling medium), contact the nearest authorized Sakura distributor or representative. 2-38...
  • Page 108 2-39...

  • Page 109: Chapter 3 Maintenance/Inspection

    Chapter 3 Maintenance/Inspection...

  • Page 110: Daily Care And Inspection

    Daily Care and Inspection Inspect and clean the instrument periodically to prevent malfunction and breakdown. Unless otherwise specified in this document, be sure to carry out all inspection and cleaning tasks with the blade guard put on and handwheel lock tab locked. Cleaning the Chamber If debris is collected in the chamber, operations may be affected and tissue samples may also be contaminated/infected.

  • Page 111: Setting The Waste Tank

    Setting the Waste Tank This tank is used to collect water that generates as a result of defrost cycle. Put the hose into the tank port and set it in the Polar® instrument storage space. Make sure the tank is empty.

  • Page 112: How To Install The Vacuum Filter Set

    Daily Care and Inspection How to Install the Vacuum Filter Set Connection port on Vacuum instrument side connection port The vacuum function is provided to suction debris, etc., to keep the interior of the chamber clean. The vacuum head connection hose is connected to the vacuum hose attachment port on the blade holder to suction debris on the stage while sectioning the specimen.

  • Page 113: Replacing The Vacuum Filter Set

    Replacing the Vacuum Filter Set When the vacuum filter is used for a long period of time or is used to filter a large amount of sections, its suction capacity may drop or infections may occur. Replace the vacuum filter every 3 months or after suctioning every 2,000 sections.

  • Page 114: How To Set The Draft Generator

    Blue (level 5, steady This is a software error. Contact light) the Sakura authorized distributor. Suction force change switch * If the particular situation you encountered is not listed above, contact the nearest authorized Sakura distributor or representative. LED lamp (green or red)

  • Page 115: Replacing The Draft Generator Filter

    Replacing the Draft Generator Filter The filter capacity drops after a long period of use. As a rough guide, replace the filter every 2 years. If a clogged filter is detected, both the green and red LED lamps on the draft generator turn to steady light.

  • Page 116: How To Disassemble/Set And Clean The Blade Holder

    Daily Care and Inspection How to Disassemble/Set and Clean the Blade Holder By disassembling and cleaning the blade holder, debris attached to the blade holder can be removed effectively. Clean the blade holder with the disposable blade removed. Refer to p. 3-9 for information on disassembling the Anti-Roll Plate. Be careful not to lose the wave washer when cleaning the blade holder.

  • Page 117: Disassembling/Cleaning The Stage

    Stage Disassembling/Cleaning the Stage The stage is a black part that presses down the disposable blade and carries the cut section. Disassemble and clean the stage as debris and trimmings collect in it. 1. Tilt toward you the disposable blade mounting lever indicated in red, to unlock and pull out the lever [1].

  • Page 118: How To Disassemble/Set And Clean The Anti-Roll Plate

    Daily Care and Inspection How to Disassemble/Set and Clean the Anti-Roll Plate Debris attached to the Anti-Roll Plate can be removed effectively by disassembling/cleaning the Anti-Roll Plate. How to Remove the Anti-Roll Plate and Vacuum Suction Port 1. The Anti-Roll Plate is locked to the blade holder with the vacuum suction port, vacuum hose attachment port and screws.

  • Page 119: Disassembling And Assembling The Anti-Roll Plate

    Disassembling and Assembling the Anti-Roll Plate The removed Anti-Roll Plate can be disassembled for easy care. 1. Turn the black knurled screw and pull it out [1]. 2. Remove the vacuum suction port [2]. 3. When the left mounting plate is removed [3], the disassembly is complete. 4.

  • Page 120: Cleaning The Anti-Roll Rake

    The ozone generation capacity of the lamp drops after a long period of use, and the ozone disinfection performance of the instrument drops as a result. Replace the ozone lamp after around 1,000 times of use or every year. If you want to replace the ozone lamp, contact the nearest authorized Sakura distributor or representative.

  • Page 121: Cleaning The Air Intake Grille

    When the preset replacement time comes close, the instrument displays the replacement notice automatically. Contact the nearest authorized Sakura distributor or representative for replacement of the brine solution, when needed. 3-12...
  • Page 122 3-13...

  • Page 123: Troubleshooting

    Chapter 4 Troubleshooting...

  • Page 124: Before Suspecting A Trouble ~ Instrument Conditions And Remedial Actions

    If the problem cannot be resolved or the applicable condition is not listed, contact the nearest authorized Sakura distributor or representative. * If an unexpected problem occurred and the condition cannot be rectified easily, turn off the power immediately. Contact the nearest authorized Sakura distributor or representative. Reference Symptom...
  • Page 125 Wait for the ozone disinfection cycle to complete. If the window still does not open after the remaining ozone disinfection time reaches "0", contact the nearest authorized Sakura distributor or representative. The home position Is the specimen head at the top position?
  • Page 126 Troubleshooting Reference Symptom Check Action Page The object holder Is debris accumulated on or around the Clean the specimen holder. cannot be secured in specimen head setting position and/or the object the specimen head of holder placement surface? the specimen holder. Has the object holder gotten smaller due to Replace the object holder with new one.

  • Page 127: When Error/Alarm/Other Message Screens Appear

    OK button The details of the maintenance are displayed. This button resets the maintenance screen. * If an unexpected problem occurred and the condition cannot be rectified easily, turn off the power immediately. Contact the nearest authorized Sakura distributor or representative.

  • Page 128: Error Codes Displayed During System Operation

    Utilize this information to identify the system condition and resolve the problem. If the problem cannot be resolved or the applicable condition is not listed, contact the nearest authorized Sakura distributor or representative. Each value in the table indicates the applicable error number.
  • Page 129 If the window is open, close the window. Clean chamber negative the gasket contacting the window, if dirty. If the pressure/negative pressure problem persists, contact the Sakura authorized cancellation. distributor. 320, 321, 331 PRESSURE SENSOR The pressure sensor status change Contact the Sakura authorized distributor.

  • Page 130: Glossary Of Terms

    Glossary of Terms Explanation of Terms Cleaning operation Periodic defrost cycle and ozone cycle. Cleaning operation is performed when always-on operation is set. Defrost Function to defrost by raising the temperature in the chamber. Draft generator Function to suction air in the top part of the chamber from both sides of the window when the window is opened, to keep contaminated air from diffusing out of the chamber.

  • Page 131: Decontamination Statement

    Decontamination Statement Before you return the instrument that has been used in your institute to the authorized Sakura distributor or representative for any reason, please kindly complete this document and place it visibly on the outside of or include in the transport container of the instrument to be returned.

  • Page 132: Warranty Period And Repair

    If the instrument malfunctions within the warranty period despite being used in a proper condition according to the information provided in this Operating Manual, contact the nearest authorized Sakura distributor or representative. We will repair/adjust the malfunctioning location or replace the faulty part free of charge.

This manual is also suitable for:

Tissue-tek polar d

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