Sakura Tissue-Tek Film 4740 Operating Manual
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Sakura Tissue-Tek Film 4740 Operating Manual

Automated coverslipper
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  • Page 2 © 2013 Sakura Finetek USA, Inc. All Rights Reserved Printed in U.S.A. Manufactured for: Sakura Finetek USA, Inc., Torrance, CA 90501 U.S.A. Sakura Finetek Japan Co., Ltd., Tokyo, 135-0007, Japan Sakura Finetek Europe B.V., 2408 AV Alphen aan den Rijn, NL 0002470-01 Rev. F...

  • Page 3: Table Of Contents

    TABLE OF CONTENTS Section Page INTRODUCTION Intended Use ..............................1.1 Safety Instructions ............................1.1 Safety Precautions ............................1.2 General Description ............................1.1 Physical Characteristics..........................1.3 Description of Control Panel ......................... 1.7 Specifications ............................... 1.8 INSTALLATION General Information ............................2.1 Environmental Factors ..........................2.1 Unpacking ..............................

  • Page 5: Introduction

    25 x 75 mm (1 x 3 inch) microscope slides (Figure 1-A). The use of a special, resin-coated, film and xylene eliminates the need for cover glass and mounting media. (Sakura can only recommend the use of Tissue-Tek Coverslipping Film because of its proven ®...

  • Page 6: Safety Instructions

    INTRODUCTION Safety Instructions Safety Precautions The Tissue-Tek Film Automated Coverslipper CAUTIONS and NOTES are provided throughout this ® ® operating manual includes important instructions and manual to indicate levels of potential hazards or helpful information related to the operating safety and information as defined below.

  • Page 7: Physical Characteristics

    Section 1 Physical Characteristics Loading Area Access to the loading area (Figure 1-B) is obtained by opening the door located at the front, lower portion of the instrument. The loading drawer can be accessed by gently sliding the drawer out. This drawer should be filled with xylene to prevent slides from drying out prior to coverslipping.
  • Page 8 INTRODUCTION Dispensing Mechanism Xylene is pumped from the xylene bottle by a pump and then dispensed onto the slide. The xylene bottle is a glass bottle (Figure 1-D) with a capacity of 500 ml. It is located on the bottom left of the instrument and can be accessed by opening the film door.
  • Page 9 Section 1 Fume Control Xylene fumes are absorbed by a carbon filtration system (Figure 1-G). The treated air is then force-vented through the exhaust outlet. The two activated carbon cartridges are located in the unloading area behind the 12- position unloading unit. An external port (OD: 38 mm or 75 mm), an optional accessory, is available so the instrument can be vented to an outside source.
  • Page 10 INTRODUCTION Rear of the Instrument The power cord can be installed on the rear of the instrument ( .. , Figure 1-I). The other end of the power cord attaches into an appropriate grounded AC electrical outlet. There are two fuses .. located to the left of the power cord inlet.

  • Page 11: Description Of Control Panel

    Section 1 Description of Control Panel The [Exit] key is pressed to return to the previous screen without saving any changed settings. Once the power is turned on, the instrument will Directly beneath these three keys are the left [<] and initialize and perform a series of system checks as it right [>] arrow keys.

  • Page 12: Specifications

    INTRODUCTION Specifications Processing Speed Models Maximum throughput: 1,080 slides per hour 60 slides/batch Model Name/Description Acceptable Slide Dimensions 4740 Tissue-Tek Film Coverslipper (115 VAC) (USA) ® ® 4741 Tissue-Tek ® Film ® Coverslipper (100 VAC) (Asia) Dimensions: 24.7 to 26.5 mm (W) x 74.7 to 76.5 mm (L) 4742 Tissue-Tek Film...

  • Page 13: Installation

    If visible signs of damage are evident, immediately file a complaint with the carrier Environmental Factors and notify Sakura Finetek USA Technical Support Department. In countries other than the USA, contact As with all sensitive electronic instruments, prolonged...
  • Page 14 INSTALLATION 3. Remove the lid of the box and remove the accessory 6. Carefully lift the coverslipper from the pallet and place box positioned under the lid (Figure 2-C). it on a sturdy, level surface capable of supporting 75 kg (165 lbs.). CAUTION: The instrument is very heavy and large;...
  • Page 15 Section 2 Removing the Metal Packing Piece Slide Baskets (10) Slide Basket Adapters (10) CAUTION: Make sure that the instrument is switched off and that the power cord is unplugged before Film (1 roll) beginning. Cutter Blades (5) 1. Open the film door (located on the left side of the Loading Drawer (1) instrument).
  • Page 16 INSTALLATION Installing the Activated Carbon Filters Installing the 12-Position Unloading Unit CAUTION: Make sure that the instrument is switched CAUTION: Make sure that the instrument is switched off and the power cord is unplugged before off and the power cord is unplugged before beginning.
  • Page 17 Section 2 Installing the Xylene Bottle Installing the Xylene Waste Bottle CAUTION: Make sure that the instrument is switched CAUTION: Make sure that the instrument is switched off and the power cord is unplugged before off and the power cord is unplugged before beginning.
  • Page 18 INSTALLATION Installing the Cutter Blade 6. Holding the blade against the safety handle, carefully insert the blade into the slit of the guide piece on the CAUTION: Make sure that the instrument is switched blade assembly (Figure 2-L). Push the blade off and the power cord is unplugged before completely in without dragging the sharp edge of the beginning.
  • Page 19 Section 2 Installing the Loading Station 6. Slide the loading drawer back into the instrument being certain that the loading station is in the proper CAUTION: Make sure that the instrument is switched position. off and the power cord is unplugged before 7.
  • Page 20 INSTALLATION Installing the Waste Tray 1. Open the film door located on the left side of the instrument. 2. Locate the guide rails for the waste tray underneath the Xylene bottle and the Xylene waste bottle 3. Position the waste tray in between the guide rails (Figure 2-O) and push the tray completely in.
  • Page 21 1. Connect one end of the data cable to the link inlet (Figure 2-Q) located on the rear of the instrument, and the other end to the bottom right side of the Prisma Slide Stainer. NOTE: Optional Link Station must be installed by an authorized Sakura Representative. Figure 2-Q Revised 08/08/2013...

  • Page 22: Preparation Of Slides Prior To Coverslipping

    INSTALLATION Preparation of Slides Prior to This section states the importance of water and alcohol removal from the slide. Dehydration in three (3) changes Coverslipping of 100% alcohol for a minimum of 1 minute in each alcohol is a recommended practice for proper dehydration. Only stains that can be coverslipped with a solvent- Additionally, it is essential that the last 100% alcohol is based mounting medium should be used to stain slides...

  • Page 23: Operating Instructions

    Section 3 OPERATING INSTRUCTIONS Storage of Unused Film The Tissue-Tek Film Coverslipper is capable of ® ® performing continuous, rapid coverslipping by simple Avoid storing Film in humid areas. Storage conditions operating steps. for Film prior to use: Loading of Slides Relative Humidity: 30 to 70% Temperature: 10º...

  • Page 24: Setup Procedures

    OPERATING INSTRUCTIONS 5. Confirm that the loading station is positioned correctly There are seven options available from the Setup (see loading station installation). Menu: 6. Execute the priming function. If xylene bottle has not 1. Prime Volume already been positioned, it should be filled and set in 2.
  • Page 25 Section 3 Description of the Setup Menu Options a. Press the [<] and [>] keys to select option, “2. Film Length”, and press the [ENTER] key. The 1. Prime Volume The prime volume refers to the amount of xylene dispensed on each slide during coverslipping. The priming <Film Length>...
  • Page 26 OPERATING INSTRUCTIONS NOTE: If stopping the prime is required, press the [STOP] 6. Alarm Volume key to abort the prime. If there are bubbles detected in the There are four available alarm levels to choose from. priming line, Extended Prime will need to be repeated. Level 4 is the default setting.

  • Page 27: Coverslipping

    Section 3 Coverslipping 5. A basket can be added if the [LOAD] LED on the control panel is lit. Repeat steps one through 4 to add 1. Confirm that the [LOAD] LED located on the right side an additional basket. of the control panel is lit.

  • Page 28: Operating Precautions

    OPERATING INSTRUCTIONS CAUTION: Open the cover only when it is required. It 4. Close all doors and covers. is important to minimize the amount of time that the 5. Press the power switch to the “I” position. cover is opened due to reagent fume exposure. 6.
  • Page 29 Section 3 3. If needed, remove any remaining baskets from the loading station. 4. After all needed slides and baskets have been removed from inside the instrument, operation can be resumed. The following message displays: <Hold> Abort Operation? [START] to restart [STOP] to abort The instrument will be in a “Hold”...
  • Page 30 OPERATING INSTRUCTIONS Revised 08/08/2013...

  • Page 31: Slide Handling

    Section 4 SLIDE HANDLING Handling of Coverslipped Slides Equipment and Reagents Needed Coplin jars Removal of Immersion Oil Acetone Absolute alcohol Immersion oil can be removed from a coverslipped Xylene-reagent grade slide by dipping the slide into xylene for a few seconds, Forceps or teasing needle draining, and wiping the slide dry with a soft, nonabrasive, low-lint cloth.
  • Page 32 1 and 2. Review staining procedures and address deviations from 4. Immediately place the slide in xylene for one minute Sakura recommendations as immediately as possible. and gently agitate. Technical support is also available at 800-725-8723, 5.

  • Page 33: Accessories

    Waste Bottle Cap 4749 4768 20-Slide Basket Duct Connection 6166 Adapter, 75mm dia 4770 Coverslipping Film Bar Code Reader (must be installed by 6508 Sakura Technical Support) 4772 Cutter Blades 20-Slide Basket 6136 Adapter Activated Carbon 6160 Filters Revised 08/08/2013...
  • Page 34 ACCESSORIES Revised 08/08/2013...

  • Page 35: Care Of The Instrument

    Section 6 CARE OF THE INSTRUMENT General Maintenance Film Waste Tray The film waste tray should be emptied and cleaned at Keep the exterior of the Tissue-Tek Film Automated ® ® least once each month. Coverslipper free of dust at all times. If needed, the 1.
  • Page 36 CARE OF THE INSTRUMENT Inspect the slide baskets for wear and evidence of stress weekly. The molded plastic material will wear with normal instrument operation and show signs of stress if heated to high temperatures during slide drying procedures. Discard and replace the stressed baskets immediately to prevent slide delivery and receiving malfunctions.

  • Page 37: Troubleshooting

    If additional assistance is required concerning an instrument problem, or if the problem cannot be isolated or is beyond the scope of this manual, please contact the Sakura Finetek USA, Technical Support Department. If outside the USA, call the nearest Sakura representative. Revised 08/08/2013...

  • Page 38: Messages

    If the situation cannot be resolved by adhering to the appropriate actions described below, it will be necessary Various messages may be displayed if the instrument to contact the Sakura Finetek USA, Technical Support has operated erroneously or if necessary operational Department.
  • Page 39 Coverslipping, paragraph 3. Fan failure A fault (stop) of fume control system fan is detected. The Press the [EXIT] key and the screen will disappear. If system continues operation. this Warning continues, contact Sakura Technical support. Revised 08/08/2013...
  • Page 40 Motor Error (Ejector) Press the STOP key. The robotic arm should return to the home position after the Motor Error (Arm) error recovery. If the error cannot be recovered, please contact Sakura Technical Motor Error (Loading X) Support Department for assistance.

  • Page 41: Service And Replacement Parts

    Bar Code Reader * Where to Call for Service If located within the United States, contact the Must be installed by Sakura Technical Support Technical Support Department of Sakura Finetek U.S.A., Inc. by calling toll free: (800) 725-8723 - Menu Option 2...
  • Page 42 SERVICE AND REPLACEMENT PARTS Revised 08/08/2013...

  • Page 43: Bar Code Reader

    Appendix A BAR CODE READER Introduction Physical Characteristics The Tissue-Tek Film Automated Film Coverslipper with Safety Precautions Barcode Reader 4743 has the USB output on the back of the unit. The other outputs are not for bar code data WARNING: DO NOT VIEW LED LIGHT DIRECTLY WITH delivery (operating manual page 2.9) See Picture below: OPTICAL INSTRUMENTS CLASS 1 LED PRODUCT LED Output: .564 mW.
  • Page 44 BAR CODE READER Weight: 2 oz. (57 g) Other Specs Types of Barcode Symbologies that can be read: Light Source: Linear (1D) barcodes Type: Code 39, Code128/EAN128, BC412, Interleaved 2 of High-output LEDs. Light Collection Options Progressive 5, Code93, Codabar, UPC/EAN, Postal Symbologies, scan, square pixel;...
  • Page 45 The bar code reader is installed and calibrated at Sakura Finetek USA, Inc. Labs that purchase the 4740 version may ad a bar code reader at a later time. This unit, part #6508, must be installed by a Sakura Service professional. Customization of Settings The Tissue-Tek Film Automated Film Coverslipper with Barcode Reader is pre-calibrated and pre-installed.

This manual is also suitable for:

Tissue-tek film 4741Tissue-tek film 4742Tissue-tek film 4743

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