Roth ROTIPHORESE PROfessional runVIEW Instructions For Use Manual page 12

Problem
No bands seen in
blue light
No Display / lights
Operation stops
Error message
Message
Alarm message
Cause
No DNA there
Too much light present
Lid not used
Buffer suppresses bands
No AC power.
AC power cord is not
connected.
The fuse has blown.
Electrophoresis leads are
not connected to the
power supply or the
electrophoresis unit; or
the circuit is broken in the
electrophoresis system.
High resistance due to
tape left on a pre-cast
gel, incorrect buffer
concentration, or
insufficient buffer
volumes in the
electrophoresis system.
Over voltage (170 V
safety limit reached or
exceeded).
No load is detected.
Maximum power output
reached (30 W).
Solution
Check presence of DNA using a UV illuminator or
methylene blue staining
Darken the room or use a dark cloth or paper to shield
the unit from light.
Bands can only be seen through the orange runVIEW
lid.
Faint bands are not seen when using 1x TBE buffer.
Use 0.5x TBE buffer for electrophoresis.
Check if the power supply is unplugged, or if the AC
power source is a problem.
Check AC power cord connections at both ends. Use
the correct cords.
Replace the fuse
Check the connections to the power supply and within
electrophoresis system to make sure the connection
is intact; check the electrodes to make sure they are
intact. Close the circuit by reconnecting the cables.
Press START/STOP to restart the run.
Make sure that the tape is removed from the pre-cast
gel, that the buffers are prepared correctly, and the
recommended volume of buffer is added to the
electrophoresis unit and is covering the gel.
Press START/STOP button to clear the error
message. Contact our service department if the
problem persists.
(1) Check the connections.
(2) Check the buffer condition / buffer
Level.
Warning message for reference.
12